Towards a 'universal' nucleant for protein crystallization.

Abstract:

:The expression 'crystal clear' stems from the science of crystallography, which determines the detailed atomic structures of materials by exposing crystals to X-rays. Protein structures are pivotal to the success of rational drug design and other biotechnology applications; however, obtaining high quality crystals poses a major problem to progress. Nucleation is the first step that determines the entire crystallization process, thus control of crystal nucleation would tackle the problem at its conception. A search for a 'universal' nucleant, a substance that can induce nucleation of any protein, is therefore on-going. We report the advances that have been made in this area, highlighting the success of especially engineered as well as naturally structured surfaces.

journal_name

Trends Biotechnol

journal_title

Trends in biotechnology

authors

Saridakis E,Chayen NE

doi

10.1016/j.tibtech.2008.10.008

subject

Has Abstract

pub_date

2009-02-01 00:00:00

pages

99-106

issue

2

eissn

0167-7799

issn

1879-3096

pii

S0167-7799(08)00288-6

journal_volume

27

pub_type

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