Abstract:
:Proteins found in nature have traditionally been the most frequently used biocatalysts to produce numerous natural products ranging from commodity chemicals to pharmaceuticals. Protein engineering has emerged as a powerful biotechnological toolbox in the development of metabolic engineering, particularly for the biosynthesis of natural products. Recently, protein engineering has become a favored method to improve enzymatic activity, increase enzyme stability, and expand product spectra in natural product biosynthesis. This review summarizes recent advances and typical strategies in protein engineering, highlighting the paramount role of protein engineering in improving and diversifying the biosynthesis of natural products. Future prospects and research directions are also discussed.
journal_name
Trends Biotechnoljournal_title
Trends in biotechnologyauthors
Li C,Zhang R,Wang J,Wilson LM,Yan Ydoi
10.1016/j.tibtech.2019.12.008subject
Has Abstractpub_date
2020-07-01 00:00:00pages
729-744issue
7eissn
0167-7799issn
1879-3096pii
S0167-7799(19)30303-8journal_volume
38pub_type
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