Quality assurance mechanisms for the unregulated research environment.

abstract：:Microbes are present at every crime scene and have been used as physical evidence for over a century. Advances in DNA sequencing and computational approaches have led to recent breakthroughs in the use of microbiome approaches for forensic science, particularly in the areas of estimating postmortem intervals (PMIs), l...

journal_title：Trends in biotechnology

authors： Metcalf JL,Xu ZZ,Bouslimani A,Dorrestein P,Carter DO,Knight R

更新日期：2017-09-01 00:00:00

abstract：:Protein engineers traditionally rely on amino acid substitutions to alter the functional properties of biomacromolecules, yet have largely overlooked the potential benefits of reorganizing the polypeptide chain of a protein by circular permutation (CP). By connecting the native protein termini via a covalent linker an...

journal_title：Trends in biotechnology

authors： Yu Y,Lutz S

更新日期：2011-01-01 00:00:00

abstract：:Adsorption in stable expanded beds enables proteins to be recovered directly from particulate-containing feedstocks, such as fermentation broths and preparations of disrupted cells, without the need for prior removal of the suspended solids, which would normally result in the blockage of packed beds. The adoption of t...

journal_title：Trends in biotechnology

authors： Chase HA

更新日期：1994-08-01 00:00:00

abstract：:Starting with the Protein Data Bank (PDB) as a common ancestor, the evolution of structural databases has been driven by the rapprochement of the structural world and the practical applications. The result is an impressive number of secondary structural databases that is welcomed by structural biologists and bioinform...

journal_title：Trends in biotechnology

authors： Carugo O,Pongor S

更新日期：2002-12-01 00:00:00

abstract：:One of the central problems in bioinformatics is data retrieval and integration. The existing biological databases are geographically distributed across the Internet, complex and heterogeneous in data types and data structures, and constantly changing. With the current rapid growth of biomedical data, the challenge is...

journal_title：Trends in biotechnology

authors： Chung SY,Wong L

更新日期：1999-09-01 00:00:00

abstract：:Peptide nucleic acids (PNAs) are novel DNA mimics in which the sugar-phosphate backbone has been replaced with a backbone based on amino acids. PNAs exhibit sequence-specific binding to DNA and RNA with higher affinities and specificities than unmodified DNA. They are resistant to nuclease and protease attack in serum...

journal_title：Trends in biotechnology

authors： Buchardt O,Egholm M,Berg RH,Nielsen PE

更新日期：1993-09-01 00:00:00

abstract：:Hydrogels are 3D hydrophilic polymer networks that absorb and hold huge amounts of water. Although hydrogels have traditionally been synthesized using chemical and physical methods, rapid developments in enzyme technology that, like chemical-based methods, enable the formation of stable covalent bonds are fast emergin...

journal_title：Trends in biotechnology

authors： Guebitz GM,Nyanhongo GS

更新日期：2018-10-01 00:00:00

abstract：:Nature's most potent molecules are produced by enzyme-catalysed reactions, coupled with the natural selection of those products that possess optimal biological activity. Combinatorial biocatalysis harnesses the natural diversity of enzymatic reactions for the iterative synthesis of organic libraries. Iterative reactio...

journal_title：Trends in biotechnology

authors： Michels PC,Khmelnitsky YL,Dordick JS,Clark DS

更新日期：1998-05-01 00:00:00

abstract：:Plant-based environmental remediation, or phytoremediation, has been widely pursued in recent years as a favorable clean-up technology and is an area of intensive scientific investigation. For the vast majority of field applications, vegetative 'phyto-crops' are selected specifically for their capacity for site decont...

journal_title：Trends in biotechnology

authors： Rugh CL

更新日期：2004-10-01 00:00:00

abstract：:Neurodegenerative diseases are of major socioeconomic importance and represent an enormous challenge for the scientific and medical communities. Advances in molecular genetics during the past decade have begun to provide approaches for the establishment of animal models for these disorders using transgenic technology....

journal_title：Trends in biotechnology

authors： Theuring F,Thunecke M,Kosciessa U,Turner JD

更新日期：1997-08-01 00:00:00

abstract：:There are many aims associated with the optimization of fermentation processes. Optimization is expected to increase the yield of the final product but the process must be compliant with good manufacturing practices, the available equipment and the expected final scale of operation. Dealing with genetically modified m...

journal_title：Trends in biotechnology

authors： Thiry M,Cingolani D

更新日期：2002-03-01 00:00:00

abstract：:Patterned regular sieves and filters with comparable molecular dimensions hold great promise as an alternative to conventional polymeric gels and fibrous membranes to improve biomolecule separation. Recent developments of microfabricated nanofluidic sieves and filters have demonstrated superior performance for both an...

journal_title：Trends in biotechnology

authors： Fu J,Mao P,Han J

更新日期：2008-06-01 00:00:00

abstract：:Microalgal production technologies are seen as increasingly attractive for bioenergy production to improve fuel security and reduce CO(2) emissions. Photosynthetically derived fuels are a renewable, potentially carbon-neutral and scalable alternative reserve. Microalgae have particular promise because they can be prod...

journal_title：Trends in biotechnology

authors： Larkum AW,Ross IL,Kruse O,Hankamer B

更新日期：2012-04-01 00:00:00

abstract：:Acidity is a biomarker of cancer that is not subject to the blunting clonal selection effects that reduce the efficacy of other biomarker technologies, such as antibody targeting. The pH (low) insertion peptides (pHLIP®s) provide new opportunities for targeting acidic tissues. Through the physical mechanism of membran...

journal_title：Trends in biotechnology

authors： Wyatt LC,Lewis JS,Andreev OA,Reshetnyak YK,Engelman DM

更新日期：2017-07-01 00:00:00

abstract：:Along with the development of metabolic engineering and synthetic biology tools, various microbes are being used to produce aromatic chemicals. In microbes, aromatics are mainly produced via a common important precursor, chorismate, in the shikimate pathway. Natural or non-natural aromatics have been produced by engin...

journal_title：Trends in biotechnology

authors： Noda S,Kondo A

更新日期：2017-08-01 00:00:00

abstract：:The existence of a medicinal 'biotech revolution' has been widely accepted and promoted by academics, consultants, industry and government. This has generated expectations about significant improvements in the drug discovery process, healthcare and economic development that influence a considerable amount of policy-ma...

journal_title：Trends in biotechnology

authors： Nightingale P,Martin P

更新日期：2004-11-01 00:00:00

abstract：:This article reports on the state-of-the-art technologies that sequence DNA using miniaturized devices. The article considers the miniaturization of existing technologies for sequencing DNA and the opportunities for cost reduction that 'on-chip' devices can deliver. The ability to construct nano-scale structures and p...

journal_title：Trends in biotechnology

authors： Ryan D,Rahimi M,Lund J,Mehta R,Parviz BA

更新日期：2007-09-01 00:00:00

abstract：:Room temperature ionic liquids are non-volatile, thermally stable and highly polar; they are also moderately hydrophilic solvents. Here, we discuss their use as reaction media for biocatalysis. Enzymes of widely diverging types are catalytically active in ionic liquids or aqueous biphasic ionic liquid systems. Lipases...

journal_title：Trends in biotechnology

authors： van Rantwijk F,Madeira Lau R,Sheldon RA

更新日期：2003-03-01 00:00:00

abstract：:In the wake of public distrust regarding biotechnology, it has been suggested that the debate should be moved "upstream", whereby the public help to set research priorities. Although many scientists see this as an illogical reaction to a loss of faith in science, we argue that the boundaries between science and its te...

journal_title：Trends in biotechnology

authors： Davies KG,Wolf-Phillips J

更新日期：2006-02-01 00:00:00

abstract：:Bacteriorhodopsin (BR) is the photoactive proton pump found in the purple membrane of the salt marsh archaeon Halobacterium salinarum. Evolution has optimized this protein for high photochemical efficiency, thermal stability and cyclicity, as the organism must be able to function in a hot, stagnant and resource-limite...

journal_title：Trends in biotechnology

authors： Wise KJ,Gillespie NB,Stuart JA,Krebs MP,Birge RR

更新日期：2002-09-01 00:00:00

abstract：:The generation of genetically engineered (GE) foods has been raising several concerns and controversies that divide not only the general public but also the scientific community. The fear and importance of the new technology, as well as commercial interests, have supported many of the ongoing discussions. The recent i...

journal_title：Trends in biotechnology

authors： Batista R,Oliveira MM

更新日期：2009-05-01 00:00:00

abstract：:Tremendous advances in our understanding of the human immunodeficiency viruses (HIVs) have been made through the use of animal models. However, there are limitations inherent in many of the current models that use either nonhuman primates, or viruses other than HIV-1. Researchers continue to search for improved models...

journal_title：Trends in biotechnology

authors： Lewis AD,Johnson PR

更新日期：1995-04-01 00:00:00

abstract：:The diffusion of genetically modified (GM) animals has generated a demand for accurate and unique identification to assure compliance with relevant national and international legislation. Individual identification of GM animals is essential to improve safety and traceability, as well as to fulfill the present and futu...

journal_title：Trends in biotechnology

authors： Forabosco F,Sundström FL,Rydhmer L

更新日期：2013-05-01 00:00:00

abstract：:Nanomaterials have been developed for many biomedical applications, including medical imaging, drug delivery, and antimicrobial coatings. Intriguingly, nanoparticles can display 'enzyme-like' activity and have been explored as alternatives to natural enzymes in several industrial and energy-related applications. Recen...

journal_title：Trends in biotechnology

authors： Cormode DP,Gao L,Koo H

更新日期：2018-01-01 00:00:00

abstract：:DNA and RNA molecules can be detected as they are driven through a nanopore by an applied electric field at rates ranging from several hundred microseconds to a few milliseconds per molecule. The nanopore can rapidly discriminate between pyrimidine and purine segments along a single-stranded nucleic acid molecule. Nan...

journal_title：Trends in biotechnology

authors： Deamer DW,Akeson M

更新日期：2000-04-01 00:00:00

abstract：:Enantiomerically pure cyanohydrins are important synthetic intermediates for pharmaceuticals and agrochemicals. They are produced by enzyme-catalysed synthesis using oxynitrilases. Sufficient quantities of enzyme are available via cheap natural sources and there have been recent advances in overexpression production o...

journal_title：Trends in biotechnology

authors： Griengl H,Schwab H,Fechter M

更新日期：2000-06-01 00:00:00

abstract：:Selective delivery of lethal levels of drugs to tumors, without concomitant damage to normal tissues, is a major challenge in cancer chemotherapy. Prodrugs used in conjunction with enzyme-monoclonal antibody conjugates that can target tumors and convert prodrugs to their active drug forms in situ, offer exceptional pr...

journal_title：Trends in biotechnology

authors： Huennekens FM

更新日期：1994-06-01 00:00:00

abstract：:The free-radical gas nitric oxide (NO) plays an important role in a wide and diverse range of physiological processes. As progress is made in understanding the biological function of NO, there is growing interest in the possibility that inhibitors of NO synthase (NOS) may be of clinical use in the therapy of certain d...

journal_title：Trends in biotechnology

authors： Ogden JE,Moore PK

更新日期：1995-02-01 00:00:00

abstract：:Life science in the 21st century is developing rapidly through the structural analysis of biomolecules, the completion of the human genome sequence and the analysis of transcriptomes. The mouse transcriptome has been comprehensively analyzed using a gene discovery approach to collect full-length cDNA (FL-cDNA) clones....

journal_title：Trends in biotechnology

authors： Hayashizaki Y,Kanamori M

更新日期：2004-04-01 00:00:00

abstract：:Carotenoids are one of the most diverse classes of natural compounds. Plant carotenoids are composed of a C40 isoprenoid skeleton with or without epoxy, hydroxy and keto groups. They have fundamental roles in human nutrition as antioxidants and vitamin A precursors and their consumption is increasingly associated with...

journal_title：Trends in biotechnology

authors： Giuliano G,Tavazza R,Diretto G,Beyer P,Taylor MA

更新日期：2008-03-01 00:00:00