Abstract：
:Peptide nucleic acids (PNAs) are novel DNA mimics in which the sugar-phosphate backbone has been replaced with a backbone based on amino acids. PNAs exhibit sequence-specific binding to DNA and RNA with higher affinities and specificities than unmodified DNA. They are resistant to nuclease and protease attack in serum and cellular extracts and, thus, appear very promising as diagnostic and biomolecular probes, and possibly as antisense and antigene drugs.
journal_name
Trends Biotechnoljournal_title
Trends in biotechnologyauthors
Buchardt O,Egholm M,Berg RH,Nielsen PEdoi
10.1016/0167-7799(93)90097-Ssubject
Has Abstractpub_date
1993-09-01 00:00:00pages
384-6issue
9eissn
0167-7799issn
1879-3096pii
0167-7799(93)90097-Sjournal_volume
11pub_type
杂志文章abstract：:Genome engineering is crucial for answering fundamental questions about, and exploring practical applications of, microorganisms. Various microbial genome-engineering tools, including CRISPR/Cas-enhanced homologous recombination (HR), have been developed, with ever-improving simplicity, efficiency, and applicability. ...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2020.06.010
更新日期：2021-02-01 00:00:00
abstract：:Mutasynthesis couples the power of chemical synthesis with molecular biology to generate derivatives of medicinally valuable, natural products. Recently, this technique has been exploited by Cambridge-based biotech company Biotica Technology Ltd, and their collaborators, to generate promising new variants of the polyk...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2007.02.004
更新日期：2007-04-01 00:00:00
abstract：:In vitro selection from molecular libraries has rapidly come of age as a protein-engineering tool. Dramatic increases in protein affinity can be engineered using phage-display libraries, and specific antibodies can be selected directly from a single 'naïve' library of their genes. Repertoires of small molecules are a ...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0167-7799(94)90079-5
更新日期：1994-05-01 00:00:00
abstract：:Graphene is the basic building block of 0D fullerene, 1D carbon nanotubes, and 3D graphite. Graphene has a unique planar structure, as well as novel electronic properties, which have attracted great interests from scientists. This review selectively analyzes current advances in the field of graphene bioapplications. I...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2011.01.008
更新日期：2011-05-01 00:00:00
abstract：:Centromeres are a conundrum; although many proteins associated with centomeres are conserved from yeast to humans, the underlying DNA sequence is not. A proposed solution to this problem is that an epigenetic, largely heterochromatic, state be imposed by these proteins. Recent analysis of a human neocentromere and the...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2004.04.015
更新日期：2004-07-01 00:00:00
abstract：:Cultured mammalian cells are the main workhorses for producing biologics. The performance of these cell culture processes, in terms of both productivity and product quality attributes, is significantly influenced by cellular metabolism. Glucose is the major carbon source for cellular biosynthesis and energy generation...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2016.04.012
更新日期：2016-08-01 00:00:00
abstract：:A recent publication has reported the fabrication of a new microbial culture device containing an array of unprecedented density - a million growth chambers. This micro-Petri chip should impact on various fields, including biotechnology, ecology, food microbiology and drug development, by enabling high-throughput scre...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2008.03.007
更新日期：2008-07-01 00:00:00
abstract：:Proteins found in nature have traditionally been the most frequently used biocatalysts to produce numerous natural products ranging from commodity chemicals to pharmaceuticals. Protein engineering has emerged as a powerful biotechnological toolbox in the development of metabolic engineering, particularly for the biosy...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2019.12.008
更新日期：2020-07-01 00:00:00
abstract：:There are many aims associated with the optimization of fermentation processes. Optimization is expected to increase the yield of the final product but the process must be compliant with good manufacturing practices, the available equipment and the expected final scale of operation. Dealing with genetically modified m...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/s0167-7799(02)01913-3
更新日期：2002-03-01 00:00:00
abstract：:Many of the demands in the production of microalgae at a technical scale cannot presently be met by state-of-the-art cultivation technologies based on suspensions. Immobilized cultivation using porous substrate bioreactors (PSBRs) is characterized by a reduction of liquid reaction volumes by several orders of magnitud...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2016.06.004
更新日期：2017-02-01 00:00:00
abstract：:Direct delivery of purified Cas9 protein with guide RNA into plant cells, as opposed to plasmid-mediated delivery, displays high efficiency and reduced off-target effects. Following regeneration from edited cells, the ensuing plant is also likely to bypass genetically modified organism (GMO) legislation as the genome ...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2015.04.002
更新日期：2015-09-01 00:00:00
abstract：:Bacterial genomics has provided a plethora of potential targets for antibacterial drug discovery, however, success in the hunt for new antibiotics will hinge on selecting targets with the highest potential. A recent paper by Liu and coworkers describes a new approach to target selection that uncovers strategies used b...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2004.07.004
更新日期：2004-09-01 00:00:00
abstract：:Molecular imaging permits non-invasive visualization and measurement of molecular and cell biology in living subjects, thereby complementing conventional anatomical imaging. Herein, we review the emerging application of molecular imaging for the study of musculoskeletal biology. Utilizing mainly bioluminescence and fl...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2009.11.004
更新日期：2010-02-01 00:00:00
abstract：:Novel therapeutic strategies that promote wound healing seek to mimic the response of the body to wounding, to regenerate rather than repair injured tissues. Many synthetic or natural biomaterials have been developed for this purpose and are used to deliver wound therapeutics in a controlled manner that prevents unwan...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2006.05.001
更新日期：2006-07-01 00:00:00
abstract：:Photodynamic therapy (PDT) involves the use of a class of molecules that are activated by light, and which accumulate in, and are retained by, certain abnormal tissues. When these molecules (known as photosensitizers) are activated by light, they cause the formation of active forms of oxygen, which result in the killi...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/S0167-7799(00)88895-2
更新日期：1995-01-01 00:00:00
abstract：:Nature's most potent molecules are produced by enzyme-catalysed reactions, coupled with the natural selection of those products that possess optimal biological activity. Combinatorial biocatalysis harnesses the natural diversity of enzymatic reactions for the iterative synthesis of organic libraries. Iterative reactio...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0167-7799(98)01190-1
更新日期：1998-05-01 00:00:00
abstract：:Despite considerable progress in the development of cell culture techniques, including the development of the serum- and protein-free media that now routinely support hybridoma and mammalian cell growth, fetal bovine serum (FBS) supplemented media are still commonly used: a practice that raises ethical, scientific and...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2006.01.001
更新日期：2006-03-01 00:00:00
abstract：:With the ultimate aim to construct a living cell, bottom-up synthetic biology strives to reconstitute cellular phenomena in vitro - disentangled from the complex environment of a cell. Recent work towards this ambitious goal has provided new insights into the mechanisms governing life. With the fast-growing library of...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2018.03.008
更新日期：2018-09-01 00:00:00
abstract：:The discovery of targets that are sufficiently robust to yield marketable therapeutics is an enormous challenge. Through the years, several approaches have been used with varying degrees of success. These include target-independent screening of tumor-derived cell lines (disease-dependent), reductionist approaches to i...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s1471-1931(02)00199-4
更新日期：2002-12-01 00:00:00
abstract：:It is possible to improve the sensitivity of immunoassays by several orders of magnitude by exploiting nanoenvironmental effects. This approach can detect trace amounts of compounds and will better illuminate the presence of signal substances in biological systems. Here we describe a method for ultrasensitive immunoas...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2017.06.001
更新日期：2017-11-01 00:00:00
abstract：:Porous microcarriers are new support materials with important advantages in both industrial cell-culture processes and the culture of cells of medical importance. Porous microcarriers are now commercially available with internal architecture and surface chemistry suitable for culture of both anchorage-dependent and an...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0167-7799(90)90154-p
更新日期：1990-05-01 00:00:00
abstract：:Molecularly imprinted polymers (MIPs) are prepared by creating a three-dimensional polymeric matrix around a template molecule. After the matrix is removed, complementary cavities with respect to shape and functional groups remain. MIPs have been produced for applications in in vitro diagnostics, therapeutics and sepa...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2010.08.006
更新日期：2010-12-01 00:00:00
abstract：:Recently, several groups have published data on the shear sensitivity of suspended animal cells and the protective effect of certain polymers. These findings did not, at the time, seem to have great practical application because shear sensitivity did not cause great problems for large-scale applications in sparged and...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0167-7799(98)01209-8
更新日期：1998-08-01 00:00:00
abstract：:One of the central problems in bioinformatics is data retrieval and integration. The existing biological databases are geographically distributed across the Internet, complex and heterogeneous in data types and data structures, and constantly changing. With the current rapid growth of biomedical data, the challenge is...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0167-7799(99)01342-6
更新日期：1999-09-01 00:00:00
abstract：:Most infections are caused by bacteria, many of which are ever-evolving and resistant to nearly all available antibiotics. β-Lactams and glycopeptides are used to combat these infections by inhibiting bacterial cell-wall synthesis. This mechanism remains an interesting target in the search for new antibiotics in light...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2010.09.004
更新日期：2010-12-01 00:00:00
abstract：:Predicting the future use of biocatalysts for the transformation of organic compounds--both natural substrates and other compounds--needs to take many factors into account. To date, relatively few biotransformations have been developed to the industrial scale, primarily because there has been little economic incentive...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0167-7799(93)90079-O
更新日期：1993-11-01 00:00:00
abstract：:The tricarboxylic acid (TCA) cycle has been used for decades in the microbial production of chemicals such as citrate, L-glutamate, and succinate. Maximizing yield is key for cost-competitive production. However, for most TCA cycle products, the maximum pathway yield is lower than the theoretical maximum yield (Y(E))....
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2015.11.002
更新日期：2016-03-01 00:00:00
abstract：:Phytoremediation holds promise for in situ treatment of heavy metal contaminated soils. Recently, the benefits of combining siderophore-producing bacteria (SPB) with plants for metal removal from contaminated soils have been demonstrated. Metal-resistant SPB play an important role in the successful survival and growth...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2009.12.002
更新日期：2010-03-01 00:00:00
abstract：:Along with the great strides that have been made towards understanding cancer, has come a realization of the complexity of molecular events that lead to malignancy. Proteomics-based approaches, which enable the quantitative investigation of both cellular protein expression levels and protein-protein interactions invol...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/S0167-7799(01)01801-7
更新日期：2001-10-01 00:00:00
abstract：:We review published data on cell/gamete lyophilization. Most studies have utilized the same established protocols for cryopreservation (storage in liquid nitrogen) as for cell lyophilization (dehydration of frozen samples by water sublimation). Surveying natural lyoprotectants, we suggest trehalose and late embryogene...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2013.09.004
更新日期：2013-12-01 00:00:00
