Microbial Base Editing: A Powerful Emerging Technology for Microbial Genome Engineering.

abstract：:Overall, the meeting was timely and worthwhile. It is evident that there is a need for comparative studies to establish a firm foundation for future work. This is particularly important since it appears that the responses observed are influenced by the particular strain used for attenuation, the means of attenuation, ...

journal_title：Trends in biotechnology

authors： Curtiss R 3rd

更新日期：1990-09-01 00:00:00

abstract：:The ability to detect single-base changes is of fundamental importance in molecular genetics. This is particularly true in human genetics, where interest in linking mutations of identified genes to particular disease phenotypes is most intense, and where a demand exists for clinical diagnosis of defined mutations. In ...

journal_title：Trends in biotechnology

authors： Prosser J

更新日期：1993-06-01 00:00:00

abstract：:Innovative fermentation processes are necessary for the cost-effective production of bulk chemicals from renewable resources. Current microbial processes are either anaerobic processes, with high yield and productivity, or less-efficient aerobic processes. Oxygen utilization plays an important role in energy generatio...

journal_title：Trends in biotechnology

authors： Weusthuis RA,Lamot I,van der Oost J,Sanders JP

更新日期：2011-04-01 00:00:00

abstract：:Bacteriophages encode many distinct proteins for the successful infection of a bacterial host. Each protein plays a specific role in the phage replication cycle, from host recognition, through takeover of the host machinery, and up to cell lysis for progeny release. As the roles of these proteins are being revealed, m...

journal_title：Trends in biotechnology

authors： Santos SB,Costa AR,Carvalho C,Nóbrega FL,Azeredo J

更新日期：2018-09-01 00:00:00

abstract：:The development of yeast artificial chromosome (YAC) cloning vectors capable of carrying several hundred kilobase-pairs of DNA insert has greatly facilitated the study of complex genomes, and the cloning of large genes as single fragments. In addition, the ability to manipulate YAC sequences by homologous recombinatio...

journal_title：Trends in biotechnology

authors： Anand R

更新日期：1992-01-01 00:00:00

abstract：:The hand-held gene gun provides a rapid and efficient method of incorporating fluorescent dyes into cells, a technique that is becoming known as diolistics. Transporting fluorescent dyes into cells has, in the past, used predominantly injection or chemical methods. The use of the gene gun, combined with the new genera...

journal_title：Trends in biotechnology

authors： O'Brien JA,Lummis SC

更新日期：2007-11-01 00:00:00

abstract：:There is a need for techniques that ensure antibiotic removal in anaerobic digesters for robust methane production. In this article, we discuss recent strategies for enhanced antibiotic removal from swine wastewater and offer insights on anaerobic digestion (AD) process design for improved antibiotic removal. ...

journal_title：Trends in biotechnology

authors： Zhou Q,Li X,Wu S,Zhong Y,Yang C

更新日期：2021-01-01 00:00:00

abstract：:The best long-term prospects for the reduction in the number of deaths due to cancer lie with screening and prevention rather than with therapy of the advanced disease. Screening efficiency will be improved by the identification of individuals at inherited high risk of cancer. Understanding the mechanism of predisposi...

journal_title：Trends in biotechnology

authors： Ponder BA

更新日期：1990-04-01 00:00:00

abstract：:We have isolated several microorganisms that are adapted to living in the extremes of the deep-sea environment. They include barophilic bacteria, which are able to grow at high hydrostatic pressure, but that are unable to grow at atmospheric pressure, and organic-solvent-tolerant bacteria, which are able to grow in th...

journal_title：Trends in biotechnology

authors： Kato C,Inoue A,Horikoshi K

更新日期：1996-01-01 00:00:00

abstract：:Building robust manufacturing processes from biological components is a task that is highly complex and requires sophisticated tools to describe processes, inputs, and measurements and administrate management of knowledge, data, and materials. We argue that for bioengineering to fully access biological potential, it w...

journal_title：Trends in biotechnology

authors： Sadowski MI,Grant C,Fell TS

更新日期：2016-03-01 00:00:00

abstract：:Many bacteria and fungi produce natural products that are toxic to other microorganisms and have a variety of physiological effects in animals. Recent studies have revealed that, in several cases, the targets of these agents are components of conserved signal-transduction cascades. This article looks at the mechanisms...

journal_title：Trends in biotechnology

authors： Cardenas ME,Sanfridson A,Cutler NS,Heitman J

更新日期：1998-10-01 00:00:00

abstract：:Peptide nucleic acids (PNAs) are novel DNA mimics in which the sugar-phosphate backbone has been replaced with a backbone based on amino acids. PNAs exhibit sequence-specific binding to DNA and RNA with higher affinities and specificities than unmodified DNA. They are resistant to nuclease and protease attack in serum...

journal_title：Trends in biotechnology

authors： Buchardt O,Egholm M,Berg RH,Nielsen PE

更新日期：1993-09-01 00:00:00

abstract：:A switch from a petroleum-based to a biobased economy requires the capacity to produce both high-value low-volume and low-value high-volume products. Recent evidence supports the development of microalgae-based microbial cell factories with the objective of establishing environmentally sustainable manufacturing soluti...

journal_title：Trends in biotechnology

authors： Butler T,Kapoore RV,Vaidyanathan S

更新日期：2020-06-01 00:00:00

abstract：:Molecular imaging permits non-invasive visualization and measurement of molecular and cell biology in living subjects, thereby complementing conventional anatomical imaging. Herein, we review the emerging application of molecular imaging for the study of musculoskeletal biology. Utilizing mainly bioluminescence and fl...

journal_title：Trends in biotechnology

authors： Reumann MK,Weiser MC,Mayer-Kuckuk P

更新日期：2010-02-01 00:00:00

abstract：:The use of antibodies or antibody fragments for targeting tumors (either for tumor imaging or as carriers for drugs or toxins), has encountered problems of clearance, and non-specific or inefficient binding in clinical trials. A novel approach, linking two antibody variable fragments (Fvs), with a short peptide to gen...

journal_title：Trends in biotechnology

authors： Bird RE,Walker BW

更新日期：1991-04-01 00:00:00

abstract：:Nature's most potent molecules are produced by enzyme-catalysed reactions, coupled with the natural selection of those products that possess optimal biological activity. Combinatorial biocatalysis harnesses the natural diversity of enzymatic reactions for the iterative synthesis of organic libraries. Iterative reactio...

journal_title：Trends in biotechnology

authors： Michels PC,Khmelnitsky YL,Dordick JS,Clark DS

更新日期：1998-05-01 00:00:00

abstract：:Recently, several groups have published data on the shear sensitivity of suspended animal cells and the protective effect of certain polymers. These findings did not, at the time, seem to have great practical application because shear sensitivity did not cause great problems for large-scale applications in sparged and...

journal_title：Trends in biotechnology

authors： van der Pol L,Tramper J

更新日期：1998-08-01 00:00:00

abstract：:Europe has led many aspects of gene research and yet it has been unable to translate these discoveries into a globally dominant industrial sector. There are valid societal, political and financial reasons for its reluctance to deploy agricultural biotechnology but this reluctance might have unintended consequences. It...

journal_title：Trends in biotechnology

authors： Enriquez J

更新日期：2001-04-01 00:00:00

abstract：:Many organisms have the ability to form spores, a remarkable phase in their life cycles. Compared with vegetative cells, spores have several advantages (e.g. resistance to toxic compounds, temperature, desiccation and radiation) making them well suited to various applications. The applications of spores that first spr...

journal_title：Trends in biotechnology

authors： Wolken WA,Tramper J,van der Werf MJ

更新日期：2003-08-01 00:00:00

abstract：:A major impact of genome projects on human health will be their contribution to the understanding of protein function. Proteins are the engines of biological systems, nearly all pharmaceuticals act on proteins and increasingly proteins themselves are used therapeutically. As biology enters the post-genomic era, resear...

journal_title：Trends in biotechnology

authors： Braun P,LaBaer J

更新日期：2003-09-01 00:00:00

abstract：:The process of studying dynamic three-dimensional samples has a long history in biological research. Recent advances in hardware and software have made it easier to visualise and record interior detail from multiple focal planes of three-dimensional samples as they change over time (four-dimensional imaging). Once cap...

journal_title：Trends in biotechnology

authors： Thomas CF,White JG

更新日期：1998-04-01 00:00:00

abstract：:Genetic engineering methods based on the use of transgenes have been successfully adopted to improve crops. A novel all-native DNA gene technology consists of the creation of intragenic constructs by isolating genetic elements from a crop, rearranging them in vitro, and inserting them back into the plant. The ever-inc...

journal_title：Trends in biotechnology

authors： Molesini B,Pii Y,Pandolfini T

更新日期：2012-02-01 00:00:00

abstract：:The free-radical gas nitric oxide (NO) plays an important role in a wide and diverse range of physiological processes. As progress is made in understanding the biological function of NO, there is growing interest in the possibility that inhibitors of NO synthase (NOS) may be of clinical use in the therapy of certain d...

journal_title：Trends in biotechnology

authors： Ogden JE,Moore PK

更新日期：1995-02-01 00:00:00

abstract：:The lantibiotics are a unique class of bacterium-derived peptide antibiotics, all of which contain the rare amino acid lanthionine, as well as a number of other nonprotein amino acids. Unlike most other peptide antibiotics, lantibiotics are produced on the ribosome as a prepeptide, and a series of post-translational m...

journal_title：Trends in biotechnology

authors： Jack RW,Sahl HG

更新日期：1995-07-01 00:00:00

abstract：:Marine natural products (NP) are unanimously acknowledged as the 'blue gold' in the urgent quest for new pharmaceuticals. Although corals are among the marine organisms with the greatest diversity of secondary metabolites, growing evidence suggest that their symbiotic bacteria produce most of these bioactive metabolit...

journal_title：Trends in biotechnology

authors： Leal MC,Calado R,Sheridan C,Alimonti A,Osinga R

更新日期：2013-10-01 00:00:00

abstract：:DNA and RNA molecules can be detected as they are driven through a nanopore by an applied electric field at rates ranging from several hundred microseconds to a few milliseconds per molecule. The nanopore can rapidly discriminate between pyrimidine and purine segments along a single-stranded nucleic acid molecule. Nan...

journal_title：Trends in biotechnology

authors： Deamer DW,Akeson M

更新日期：2000-04-01 00:00:00

abstract：:Breath-based metabolomics (breathomics) is an exciting developing area of biotechnology that centers on the capture, identification, and quantification of volatile organic compound (VOC) patterns in human breath and their utilization as tools in the diagnosis of a broad spectrum of medical problems. With the age of pe...

journal_title：Trends in biotechnology

authors： Rattray NJ,Hamrang Z,Trivedi DK,Goodacre R,Fowler SJ

更新日期：2014-10-01 00:00:00

abstract：:High-throughput genetic screens interfering with gene expression are invaluable tools to identify gene function and phenotype-to-genotype interactions. Implementing such screens in the laboratory is challenging, and the choice between currently available technologies based on RNAi and CRISPR/Cas9 (CRISPR-associated pr...

journal_title：Trends in biotechnology

authors： Schuster A,Erasimus H,Fritah S,Nazarov PV,van Dyck E,Niclou SP,Golebiewska A

更新日期：2019-01-01 00:00:00

abstract：:Techniques such as positron-emission tomography, single-photon-emission computed tomography, functional magnetic-resonance imaging and magnetoencephalography permit the observation of biological processes in the brain in a noninvasive manner. They have yielded new insights into the biological interrelations of sensory...

journal_title：Trends in biotechnology

authors： Müller-Gärtner HW

更新日期：1998-03-01 00:00:00

abstract：:Cyanobacteria promise to be an important industrial platform for the production of a variety of biobased products such as fuels, plastics, and isoprenoids. Recent advances in synthetic biology have resulted in various cyanobacterial strain improvements. Nevertheless, these new strains are still hampered by product inh...

journal_title：Trends in biotechnology

authors： van den Berg C,Eppink MHM,Wijffels RH

更新日期：2019-05-01 00:00:00