Abstract：
:The hand-held gene gun provides a rapid and efficient method of incorporating fluorescent dyes into cells, a technique that is becoming known as diolistics. Transporting fluorescent dyes into cells has, in the past, used predominantly injection or chemical methods. The use of the gene gun, combined with the new generation of fluorescent dyes, circumvents some of the problems of using these methods and also enables the study of cells that have proved difficult traditionally to transfect (e.g. those deep in tissues and/or terminally differentiated); in addition, the use of ion- or metabolite-sensitive dyes provides a route to study cellular mechanisms. Diolistics is also ideal for loading cells with optical nanosensors--nanometre-sized sensors linked to fluorescent probes. Here, we discuss the theoretical considerations of using diolistics, the advantages compared with other methods of inserting dyes into cells and the current uses of the technique, with particular consideration of nanosensors.
journal_name
Trends Biotechnoljournal_title
Trends in biotechnologyauthors
O'Brien JA,Lummis SCdoi
10.1016/j.tibtech.2007.07.014subject
Has Abstractpub_date
2007-11-01 00:00:00pages
530-4issue
11eissn
0167-7799issn
1879-3096pii
S0167-7799(07)00240-5journal_volume
25pub_type
杂志文章,评审abstract：:Diatoms are unicellular, eukaryotic, photosynthetic algae that are found in aquatic environments. Diatoms have enormous ecological importance on this planet and display a diversity of patterns and structures at the nano- to millimetre scale. Diatom nanotechnology, a new interdisciplinary area, has spawned collaboratio...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2008.11.003
更新日期：2009-02-01 00:00:00
abstract：:Despite considerable progress in the development of cell culture techniques, including the development of the serum- and protein-free media that now routinely support hybridoma and mammalian cell growth, fetal bovine serum (FBS) supplemented media are still commonly used: a practice that raises ethical, scientific and...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2006.01.001
更新日期：2006-03-01 00:00:00
abstract：:Integrated biorefineries have the potential to contribute towards sustainable production of transportation fuels, energy, and chemicals. However, because there are currently no commercial biorefining plants in operation, it is not clear how sustainable they really are. This paper sets out to examine key issues associa...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2013.10.009
更新日期：2014-01-01 00:00:00
abstract：:Although many microorganisms have been used for the bioindustrial generation of valuable metabolites, the productive potential of cyanobacterial species has remained largely unexplored. Cyanobacteria possess several advantages as organisms for bioindustrial processes, including simple input requirements, tolerance of ...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2010.12.003
更新日期：2011-02-01 00:00:00
abstract：:The best long-term prospects for the reduction in the number of deaths due to cancer lie with screening and prevention rather than with therapy of the advanced disease. Screening efficiency will be improved by the identification of individuals at inherited high risk of cancer. Understanding the mechanism of predisposi...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0167-7799(90)90147-p
更新日期：1990-04-01 00:00:00
abstract：:Discussions on research quality and reproducibility are appearing in the pages of scientific journals with heightened significance and gaining media attention. Many institutions have developed guidelines to address the topic of quality in basic research, but questions remain about how best to implement and monitor com...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2013.06.007
更新日期：2013-10-01 00:00:00
abstract：:There are many paths toward effective microbial inoculants for agriculture. Considering what is practical for the present day technological and farming landscape should not limit our creativity in developing innovative technologies. However, factors including production costs, practicality of implementation, and techn...
journal_title：Trends in biotechnology
pub_type： 评论,信件
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更新日期：2019-06-01 00:00:00
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journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2020.07.003
更新日期：2020-08-14 00:00:00
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journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2019.12.023
更新日期：2020-06-01 00:00:00
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journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2020.06.010
更新日期：2021-02-01 00:00:00
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journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2017.11.008
更新日期：2018-07-01 00:00:00
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journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2005.06.006
更新日期：2005-09-01 00:00:00
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journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/S0167-7799(00)88925-8
更新日期：1995-04-01 00:00:00
abstract：:Although microorganisms have long been known as producers of edible oils, opportunities for their biotechnological exploitation are limited to the highest-value commodities. A recent attempt to develop a yeast oil cocoa-butter equivalent has not succeeded, not because of the inability to produce the correct formulatio...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/0167-7799(93)90015-2
更新日期：1993-07-01 00:00:00
abstract：:Antimicrobial resistance (AMR) has developed into a huge threat to global health, and reducing it is an urgent priority for public health authorities. The importance of a healthy and balanced gut microbiome has been identified as a key protective factor against AMR development, but this can be significantly affected b...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2019.10.008
更新日期：2020-04-01 00:00:00
abstract：:In Europe, in light of ethical, political and commercial pressure, every effort should be made to replace animals with alternatives (e.g. in vitro models), to reduce the number of animals used in experiments to a minimum and to refine current testing strategies in a way that ensures animals undergo minimum pain and di...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2006.06.003
更新日期：2006-08-01 00:00:00
abstract：:Psychrophilic anaerobic treatment is an attractive option for wastewaters that are discharged at moderate to low temperature. The expanded granular sludge bed (EGSB) reactor has been shown to be a feasible system for anaerobic treatment of mainly soluble and pre-acidified wastewater at temperatures of 5--10 degrees C....
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0167-7799(01)01701-2
更新日期：2001-09-01 00:00:00
abstract：:Continued progress in cell-phone devices has made them powerful mobile computers, equipped with sophisticated, permanent physical sensors embedded as the default configuration. By contrast, the incorporation of permanent biosensors in cell-phone units has been prevented by the multivocal nature of the stimuli and the ...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2014.03.007
更新日期：2014-07-01 00:00:00
abstract：:Antibodies are able to both bind antigens and trigger the responses that eliminate them from circulation. All antibodies are glycosylated at conserved positions in their constant regions, and the presence of carbohydrate can be critical for antigen clearance functions such as complement activation. The structure of th...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/S0167-7799(96)10062-7
更新日期：1997-01-01 00:00:00
abstract：:A key aspect of the genomics revolution is the transformation of large amounts of biological information into an electronic format, leading to an information-based approach to biomedical problems. Large-scale RNA assays and gene-expression-microarray studies, in particular, represent the second wave of the genomics re...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
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更新日期：1999-11-01 00:00:00
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journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2015.01.003
更新日期：2015-04-01 00:00:00
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journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0167-7799(01)01743-7
更新日期：2001-10-01 00:00:00
abstract：:Phytoremediation holds promise for in situ treatment of heavy metal contaminated soils. Recently, the benefits of combining siderophore-producing bacteria (SPB) with plants for metal removal from contaminated soils have been demonstrated. Metal-resistant SPB play an important role in the successful survival and growth...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2009.12.002
更新日期：2010-03-01 00:00:00
abstract：:Bioremediation differs from other industrial biotechnologies in that, although bioremediation contractors must profit from the activity, the primary driver is regulatory compliance rather than manufacturing profit. It is an attractive technology in the context of a bioeconomy but currently has limitations at the field...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2013.01.015
更新日期：2013-06-01 00:00:00
abstract：:Two of the primary responses in a cell when perturbed are modulation of the dynamics of its constituent gene expression and protein abundance to restore steady-state homeostasis. To obtain a detailed model of the restoration of this balance, it is necessary to examine the kinetics of transcription and translation, thu...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2012.09.007
更新日期：2012-12-01 00:00:00
abstract：:Intravital imaging enables to study dynamic tumour-stroma interactions within primary and metastatic sites, including the lung. The combination of optical windows with specific molecular probes targeting the tumour microenvironment will provide new insights into prometastatic stromal interactions and lead to novel the...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2016.05.001
更新日期：2017-01-01 00:00:00
abstract：:Starting with the Protein Data Bank (PDB) as a common ancestor, the evolution of structural databases has been driven by the rapprochement of the structural world and the practical applications. The result is an impressive number of secondary structural databases that is welcomed by structural biologists and bioinform...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0167-7799(02)02082-6
更新日期：2002-12-01 00:00:00
abstract：:Despite the wealth of research on stem-cell biology and therapy, there have been few advances in the ex vivo expansion of adult stem cells. Fetal bovine serum, which can elicit an immune reaction in hosts receiving cellular products, remains essential to the culture methods. Recent work by Prockop et al. suggests that...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2004.05.006
更新日期：2004-08-01 00:00:00
abstract：:Graphene is the basic building block of 0D fullerene, 1D carbon nanotubes, and 3D graphite. Graphene has a unique planar structure, as well as novel electronic properties, which have attracted great interests from scientists. This review selectively analyzes current advances in the field of graphene bioapplications. I...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2011.01.008
更新日期：2011-05-01 00:00:00
abstract：:A major goal of material science is to produce hierarchical materials that are ordered on all length scales, from the molecular (1-100 A) via the nano (10-100 nm) to the meso (1-100 microm). In these materials, the larger-scale properties can be controlled by choosing molecular characteristics. Methods developed to pr...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/s0167-7799(00)01482-7
更新日期：2000-09-01 00:00:00
