Abstract：
:High-throughput genetic screens interfering with gene expression are invaluable tools to identify gene function and phenotype-to-genotype interactions. Implementing such screens in the laboratory is challenging, and the choice between currently available technologies based on RNAi and CRISPR/Cas9 (CRISPR-associated protein 9) is not trivial. Identifying reliable candidate hits requires a streamlined experimental setup adjusted to the specific biological question. Here, we provide a critical assessment of the various RNAi/CRISPR approaches to pooled screens and discuss their advantages and pitfalls. We specify a set of best practices for key parameters enabling a reproducible screen and provide a detailed overview of analysis methods and repositories for identifying the best candidate gene hits.
journal_name
Trends Biotechnoljournal_title
Trends in biotechnologyauthors
Schuster A,Erasimus H,Fritah S,Nazarov PV,van Dyck E,Niclou SP,Golebiewska Adoi
10.1016/j.tibtech.2018.08.002subject
Has Abstractpub_date
2019-01-01 00:00:00pages
38-55issue
1eissn
0167-7799issn
1879-3096pii
S0167-7799(18)30230-0journal_volume
37pub_type
杂志文章,评审abstract：:Nature's most potent molecules are produced by enzyme-catalysed reactions, coupled with the natural selection of those products that possess optimal biological activity. Combinatorial biocatalysis harnesses the natural diversity of enzymatic reactions for the iterative synthesis of organic libraries. Iterative reactio...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0167-7799(98)01190-1
更新日期：1998-05-01 00:00:00
abstract：:The prevalence of antimicrobial-resistant pathogens has highlighted the urgent need for new drugs. Actinomycetes have been the most prominent sources of natural products for drug discovery and development. Advances in genomics have inspired several emerging strategies to reinvigorate the field of natural product disco...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2017.10.009
更新日期：2018-03-01 00:00:00
abstract：:Adsorption in stable expanded beds enables proteins to be recovered directly from particulate-containing feedstocks, such as fermentation broths and preparations of disrupted cells, without the need for prior removal of the suspended solids, which would normally result in the blockage of packed beds. The adoption of t...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0167-7799(94)90046-9
更新日期：1994-08-01 00:00:00
abstract：:The best long-term prospects for the reduction in the number of deaths due to cancer lie with screening and prevention rather than with therapy of the advanced disease. Screening efficiency will be improved by the identification of individuals at inherited high risk of cancer. Understanding the mechanism of predisposi...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0167-7799(90)90147-p
更新日期：1990-04-01 00:00:00
abstract：:Opinions on what synthetic biology actually is range from a natural extension of genetic engineering to a new manufacturing paradigm. It offers, for the first time in the life sciences, rational design and engineering standardisation. It could address problems across a broad spectrum of human concerns, including energ...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2013.01.011
更新日期：2013-05-01 00:00:00
abstract：:The application of recombinant-DNA methods for the production of therapeutic proteins has, over the past decade, driven the development of new technology for the analysis and characterization of biological molecules. High performance capillary electrophoresis (HPCE) has generated enormous interest among biochemists, a...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0167-7799(91)90078-v
更新日期：1991-07-01 00:00:00
abstract：:One of the goals of synthetic biology is to build regulatory circuits that control cell behavior, for both basic research purposes and biomedical applications. The ability to build transcriptional regulatory devices depends on the availability of programmable, sequence-specific, and effective synthetic transcription f...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2015.12.014
更新日期：2016-07-01 00:00:00
abstract：:The bioeconomy concept is proliferating globally. However, the enabling roles of biotechnology may be getting sidelined in the strategies of some countries. A goal for engineering biology is alignment with the engineering design cycle to enable more rapid commercialization. This paper considers several policy options ...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2019.03.017
更新日期：2019-09-01 00:00:00
abstract：:Molecular imaging permits non-invasive visualization and measurement of molecular and cell biology in living subjects, thereby complementing conventional anatomical imaging. Herein, we review the emerging application of molecular imaging for the study of musculoskeletal biology. Utilizing mainly bioluminescence and fl...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2009.11.004
更新日期：2010-02-01 00:00:00
abstract：:Plant-based environmental remediation, or phytoremediation, has been widely pursued in recent years as a favorable clean-up technology and is an area of intensive scientific investigation. For the vast majority of field applications, vegetative 'phyto-crops' are selected specifically for their capacity for site decont...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2004.08.003
更新日期：2004-10-01 00:00:00
abstract：:Despite the recognized potential of viral vectors for gene therapy, growing biological concerns are prompting the exploration of safer, non-viral vectors to deliver therapeutic nucleic acids. In this context, recombinant proteins can be bioproduced on a large scale, without the need for further in vitro modifications,...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2004.05.004
更新日期：2004-07-01 00:00:00
abstract：:Challenges in demonstrating interchangeability and safety, as well as the ongoing evolution of regulations governing biosimilars, have meant that the development of the biosimilars industry has not been, and will not be, a carbon copy of the generics industry. Complexity in the development process reduces the cost adv...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2015.10.009
更新日期：2016-01-01 00:00:00
abstract：:The diffusion of genetically modified (GM) animals has generated a demand for accurate and unique identification to assure compliance with relevant national and international legislation. Individual identification of GM animals is essential to improve safety and traceability, as well as to fulfill the present and futu...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2013.02.001
更新日期：2013-05-01 00:00:00
abstract：:In 2006, the group of Dr C.M. Lieber pioneered the field of nanowire sensors by fabricating devices for the ultra-sensitive label-free detection of biological macromolecules. Since then, nanowire sensors have demonstrated their ability to detect cancer-associated analytes in peripheral blood, tumor tissue, and the exh...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2018.07.014
更新日期：2019-01-01 00:00:00
abstract：:Animals exploit soft structures to move effectively in complex natural environments. These capabilities have inspired robotic engineers to incorporate soft technologies into their designs. The goal is to endow robots with new, bioinspired capabilities that permit adaptive, flexible interactions with unpredictable envi...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2013.03.002
更新日期：2013-05-01 00:00:00
abstract：:A major impact of genome projects on human health will be their contribution to the understanding of protein function. Proteins are the engines of biological systems, nearly all pharmaceuticals act on proteins and increasingly proteins themselves are used therapeutically. As biology enters the post-genomic era, resear...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/S0167-7799(03)00189-6
更新日期：2003-09-01 00:00:00
abstract：:Small interfering RNAs (siRNAs) have been shown to effectively downregulate gene expression in human cells, giving them potential to eradicate disease. Prospects for clinical applications are discussed in this review, along with an overview of recent history and our current understanding of siRNAs used for therapeutic...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2010.07.009
更新日期：2010-11-01 00:00:00
abstract：:The complex environment in the bladder weakens the efficacy of intravesical therapy. Hydrogel-based drug delivery systems are poised to revolutionize the delivery of therapeutic agents to bladder lesion sites. This forum article highlights the prospective applications of hydrogels as drug reservoirs in treating chroni...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2019.12.012
更新日期：2020-06-01 00:00:00
abstract：:Nanosilver (NS), comprising silver nanoparticles, is attracting interest for a range of biomedical applications owing to its potent antibacterial activity. It has recently been demonstrated that NS has useful anti-inflammatory effects and improves wound healing, which could be exploited in developing better dressings ...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2010.07.006
更新日期：2010-11-01 00:00:00
abstract：:In this article, the current status of membrane bioreactor (MBR) technology for wastewater treatment is reviewed. Fundamental facets of the MBR process and membrane and process configurations are outlined and the advantages and disadvantages over conventional suspended growth-based biotreatment are briefly identified....
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2007.11.005
更新日期：2008-02-01 00:00:00
abstract：:The development of yeast artificial chromosome (YAC) cloning vectors capable of carrying several hundred kilobase-pairs of DNA insert has greatly facilitated the study of complex genomes, and the cloning of large genes as single fragments. In addition, the ability to manipulate YAC sequences by homologous recombinatio...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0167-7799(92)90165-r
更新日期：1992-01-01 00:00:00
abstract：:In the wake of public distrust regarding biotechnology, it has been suggested that the debate should be moved "upstream", whereby the public help to set research priorities. Although many scientists see this as an illogical reaction to a loss of faith in science, we argue that the boundaries between science and its te...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2005.12.007
更新日期：2006-02-01 00:00:00
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journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2013.06.004
更新日期：2013-10-01 00:00:00
abstract：:The generation of genetically engineered (GE) foods has been raising several concerns and controversies that divide not only the general public but also the scientific community. The fear and importance of the new technology, as well as commercial interests, have supported many of the ongoing discussions. The recent i...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2009.01.005
更新日期：2009-05-01 00:00:00
abstract：:The genome-sequencing projects are providing a detailed 'parts list' of life. A key to comprehending this list is understanding the function of each gene and each protein at various levels. Sequence-based methods for function prediction are inadequate because of the multifunctional nature of proteins. However, just kn...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0167-7799(99)01398-0
更新日期：2000-01-01 00:00:00
abstract：:Room temperature ionic liquids are non-volatile, thermally stable and highly polar; they are also moderately hydrophilic solvents. Here, we discuss their use as reaction media for biocatalysis. Enzymes of widely diverging types are catalytically active in ionic liquids or aqueous biphasic ionic liquid systems. Lipases...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/S0167-7799(03)00008-8
更新日期：2003-03-01 00:00:00
abstract：:The well-studied antigen-combining sites of antibody molecules hold considerable promise as a model system for the design of bioactive peptides. These small, immunoglobulin-derived peptides can be used in the development of alternative treatments for disease and in diagnostic strategies. The general principles derived...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0167-7799(94)90038-8
更新日期：1994-09-01 00:00:00
abstract：:Geological storage of CO2 is a fast-developing technology that can mitigate rising carbon emissions. However, there are environmental concerns with the long-term storage and implications of a leak from a carbon capture storage (CCS) site. Traditional monitoring lacks clear protocols and relies heavily on physical meth...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2016.06.011
更新日期：2017-01-01 00:00:00
abstract：:The tricarboxylic acid (TCA) cycle has been used for decades in the microbial production of chemicals such as citrate, L-glutamate, and succinate. Maximizing yield is key for cost-competitive production. However, for most TCA cycle products, the maximum pathway yield is lower than the theoretical maximum yield (Y(E))....
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2015.11.002
更新日期：2016-03-01 00:00:00
abstract：:Diatoms are unicellular, eukaryotic, photosynthetic algae that are found in aquatic environments. Diatoms have enormous ecological importance on this planet and display a diversity of patterns and structures at the nano- to millimetre scale. Diatom nanotechnology, a new interdisciplinary area, has spawned collaboratio...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2008.11.003
更新日期：2009-02-01 00:00:00
