Abstract:
:With the ultimate aim to construct a living cell, bottom-up synthetic biology strives to reconstitute cellular phenomena in vitro - disentangled from the complex environment of a cell. Recent work towards this ambitious goal has provided new insights into the mechanisms governing life. With the fast-growing library of functional modules for synthetic cells, their classification and integration become increasingly important. We discuss strategies to reverse-engineer and recombine functional parts for synthetic eukaryotes, mimicking the characteristics of nature's own prototype. Particularly, we focus on large outer compartments, complex endomembrane systems with organelles, and versatile cytoskeletons as hallmarks of eukaryotic life. Moreover, we identify microfluidics and DNA nanotechnology as two technologies that can integrate these functional modules into sophisticated multifunctional synthetic cells.
journal_name
Trends Biotechnoljournal_title
Trends in biotechnologyauthors
Göpfrich K,Platzman I,Spatz JPdoi
10.1016/j.tibtech.2018.03.008subject
Has Abstractpub_date
2018-09-01 00:00:00pages
938-951issue
9eissn
0167-7799issn
1879-3096pii
S0167-7799(18)30092-1journal_volume
36pub_type
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journal_title:Trends in biotechnology
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abstract::Direct delivery of purified Cas9 protein with guide RNA into plant cells, as opposed to plasmid-mediated delivery, displays high efficiency and reduced off-target effects. Following regeneration from edited cells, the ensuing plant is also likely to bypass genetically modified organism (GMO) legislation as the genome ...
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journal_title:Trends in biotechnology
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journal_title:Trends in biotechnology
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journal_title:Trends in biotechnology
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journal_title:Trends in biotechnology
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