Abstract:
:Hydrophobic charge induction chromatography using 4-mercapto-ethyl-pyridine as the ligand is an effective method for the separation of antibodies from a variety of feedstocks. Antibodies are adsorbed in physiological conditions without preliminary concentration. Desorption occurs when the pH is lowered, thus inducing an ionic charge of the same sign to the ligand and the antibody. Antibody capture conditions are compatible with crude samples in terms of pH, conductivity, binding capacity and expression level. The final purity of the antibody is feedstock dependent, but can reach levels of purity as high as 98%. Examples of antibody separation are given and ligand structure information discussed.
journal_name
Trends Biotechnoljournal_title
Trends in biotechnologyauthors
Boschetti Edoi
10.1016/s0167-7799(02)01980-7subject
Has Abstractpub_date
2002-08-01 00:00:00pages
333-7issue
8eissn
0167-7799issn
1879-3096pii
S0167-7799(02)01980-7journal_volume
20pub_type
杂志文章,评审abstract::Microbes are present at every crime scene and have been used as physical evidence for over a century. Advances in DNA sequencing and computational approaches have led to recent breakthroughs in the use of microbiome approaches for forensic science, particularly in the areas of estimating postmortem intervals (PMIs), l...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2017.03.006
更新日期:2017-09-01 00:00:00
abstract::Since its inception a decade ago, sol-gel encapsulation has opened up an intriguing new way to immobilize biological materials. An array of substances, including catalytic antibodies, DNA, RNA, antigens, live bacterial, fungal, plant and animal cells and whole protozoa, have been encapsulated in silica, metal-oxide, o...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0167-7799(00)01457-8
更新日期:2000-07-01 00:00:00
abstract::Biohacking is a do-it-yourself citizen science merging body modification with technology. The motivations of biohackers include cybernetic exploration, personal data acquisition, and advocating for privacy rights and open-source medicine. The emergence of a biohacking community has influenced discussions of cultural v...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2018.02.011
更新日期:2018-08-01 00:00:00
abstract::High-throughput genetic screens interfering with gene expression are invaluable tools to identify gene function and phenotype-to-genotype interactions. Implementing such screens in the laboratory is challenging, and the choice between currently available technologies based on RNAi and CRISPR/Cas9 (CRISPR-associated pr...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2018.08.002
更新日期:2019-01-01 00:00:00
abstract::The baculovirus expression system has been used extensively for the expression of recombinant proteins in insect cells. Recently, recombinant baculovirus vectors engineered to contain mammalian cell-active promoter elements, have been used successfully for transient and stable gene delivery in a broad spectrum of prim...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0167-7799(01)01911-4
更新日期:2002-04-01 00:00:00
abstract::Feedstocks that deserve serious consideration for fuels and chemicals are sugarcane, corn, trees and algae. Commercialization of biomass refining is imminent but the wild claims of those who think that bioenergy can replace much of our dependence on foreign oil are appalling. It is naive to view biomass as the panacea...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2003.12.002
更新日期:2004-02-01 00:00:00
abstract::Recent innovations in the materials used for bioprinting have enabled transformative gains in the resolution and architecture of 3D-printed engineered tissues. We focus here on one of these innovations, reported by Lee et al., which lowers the resolution limit for printing soft biomaterials. ...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2019.09.003
更新日期:2019-11-01 00:00:00
abstract::Bacteriorhodopsin (BR) is the photoactive proton pump found in the purple membrane of the salt marsh archaeon Halobacterium salinarum. Evolution has optimized this protein for high photochemical efficiency, thermal stability and cyclicity, as the organism must be able to function in a hot, stagnant and resource-limite...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0167-7799(02)02023-1
更新日期:2002-09-01 00:00:00
abstract::Advances in our capacity to design and use novel strategies for achieving inducible gene expression will improve our ability to define gene function. An extremely efficient system designed by nature -- that of the regulatable phytochrome system in plants -- has provided the basis for developing a novel inducible gene ...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/s0167-7799(02)00022-7
更新日期:2003-02-01 00:00:00
abstract::The BRICS countries (Brazil, Russia, India, China, South Africa) account for 25% of global biotechnology patents. To understand the current and future landscape of the domain, it is important to better understand the capacity of these contributors. Here, we consider the thematic priorities, strategies, and key players...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2017.11.008
更新日期:2018-07-01 00:00:00
abstract::The availability of the human genome sequence has enabled the exploration and exploitation of the human genome and proteome to begin. Research has now focussed on the annotation of the genome and in particular of the proteome. With expert annotation extracted from the literature by biologists as the foundation, it has...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0167-7799(01)01598-0
更新日期:2001-05-01 00:00:00
abstract::Reconstituted viral envelopes (RVEs) are formed by solubilizing intact virus in detergent and reassembling the envelope on removal of detergent. RVEs can be formed in the presence of agents that become encapsulated and can then be utilized in vitro and in vivo for drug delivery, cell destruction, transfer of membrane ...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/0167-7799(91)90184-J
更新日期:1991-02-01 00:00:00
abstract::In vitro antibody generation technologies have now been available for two decades. Research reagents prepared via phage display are becoming available and several recent studies have demonstrated that these technologies are now sufficiently advanced to facilitate generation of a comprehensive renewable resource of ant...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2010.05.001
更新日期:2010-07-01 00:00:00
abstract::Microfluidic devices offer automation and high-throughput screening, and operate at low volumes of consumables. Although microfluidics has the potential to reduce turnaround times and costs for analytical devices, particularly in medical, veterinary, and environmental sciences, this enabling technology has had limited...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2014.04.010
更新日期:2014-07-01 00:00:00
abstract::Peptides possess several attractive features when compared to small molecule and protein therapeutics, such as high structural compatibility with target proteins, the ability to disrupt protein-protein interfaces, and small size. Efficient design of high-affinity peptide ligands via rational methods has been a major o...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2011.01.004
更新日期:2011-05-01 00:00:00
abstract::The announcement that the European Union (EU) had reached an agreement allowing Member States (MS) to ban genetically modified (GM) crops confirms that the EU has chosen to ignore the food security challenge issued to the world by the Food and Agriculture Organization of the United Nations (FAO) in 2009. The FAO sugge...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2016.04.003
更新日期:2016-07-01 00:00:00
abstract::Direct delivery of purified Cas9 protein with guide RNA into plant cells, as opposed to plasmid-mediated delivery, displays high efficiency and reduced off-target effects. Following regeneration from edited cells, the ensuing plant is also likely to bypass genetically modified organism (GMO) legislation as the genome ...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2015.04.002
更新日期:2015-09-01 00:00:00
abstract::Accurate diagnosis and prognosis is essential for cancer management but is subject to sampling and inter-observer error. In a recent study, Baker et al. compared Fourier transform infrared (FTIR) microspectroscopy with histological pathology to evaluate prostate tissue for disease severity. The authors found that bioc...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2009.09.001
更新日期:2009-12-01 00:00:00
abstract::Metabolomics embraces several strategies that aim to quantify cell metabolites in order to increase our understanding of how metabolite levels and interactions influence phenotypes. Metabolic footprinting represents a niche within metabolomics, because it focuses on the analysis of extracellular metabolites. Although ...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2008.05.008
更新日期:2008-09-01 00:00:00
abstract::Short, self-assembling peptides form a variety of stable nanostructures used for the rational design of functional devices. Peptides serve as organic templates for conjugating biorecognition elements, and assembling ordered nanoparticle arrays and hybrid supramolecular structures. We are witnessing the emergence of a ...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2011.11.001
更新日期:2012-03-01 00:00:00
abstract::Nanomaterials have been developed for many biomedical applications, including medical imaging, drug delivery, and antimicrobial coatings. Intriguingly, nanoparticles can display 'enzyme-like' activity and have been explored as alternatives to natural enzymes in several industrial and energy-related applications. Recen...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2017.09.006
更新日期:2018-01-01 00:00:00
abstract::Europe has led many aspects of gene research and yet it has been unable to translate these discoveries into a globally dominant industrial sector. There are valid societal, political and financial reasons for its reluctance to deploy agricultural biotechnology but this reluctance might have unintended consequences. It...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0167-7799(00)01572-9
更新日期:2001-04-01 00:00:00
abstract::Carotenoids are micronutrients important for human health. The continuous improvements in clustered regularly interspaced short palindromic repeats (CRISPR)-based genome-editing techniques make rapid, DNA/transgene-free and targeted multiplex genetic modification a reality, thus promising to accelerate the breeding an...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2020.12.003
更新日期:2020-12-29 00:00:00
abstract::In vitro selection from molecular libraries has rapidly come of age as a protein-engineering tool. Dramatic increases in protein affinity can be engineered using phage-display libraries, and specific antibodies can be selected directly from a single 'naïve' library of their genes. Repertoires of small molecules are a ...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/0167-7799(94)90079-5
更新日期:1994-05-01 00:00:00
abstract::The endothelium lining blood vessels expresses molecules that are restricted in their expression to a particular tissue or organ. These molecules are attractive targets for therapy and diagnosis because they allow agents to be delivered specifically to the blood vessels supplying the desired tissue. However, it is dif...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/S0167-7799(03)00079-9
更新日期:2003-05-01 00:00:00
abstract::Pressure is a thermodynamic parameter whose unique effects on biological systems are increasingly being studied in a growing number of scientific fields. As such, the effects of high pressure are currently being investigated at different levels, ranging from proteins, enzymes and viruses to microorganisms, mammalian c...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2009.04.001
更新日期:2009-07-01 00:00:00
abstract::Cyanobacteria promise to be an important industrial platform for the production of a variety of biobased products such as fuels, plastics, and isoprenoids. Recent advances in synthetic biology have resulted in various cyanobacterial strain improvements. Nevertheless, these new strains are still hampered by product inh...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2018.11.006
更新日期:2019-05-01 00:00:00
abstract::Graphene is the basic building block of 0D fullerene, 1D carbon nanotubes, and 3D graphite. Graphene has a unique planar structure, as well as novel electronic properties, which have attracted great interests from scientists. This review selectively analyzes current advances in the field of graphene bioapplications. I...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2011.01.008
更新日期:2011-05-01 00:00:00
abstract::With the ultimate aim to construct a living cell, bottom-up synthetic biology strives to reconstitute cellular phenomena in vitro - disentangled from the complex environment of a cell. Recent work towards this ambitious goal has provided new insights into the mechanisms governing life. With the fast-growing library of...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2018.03.008
更新日期:2018-09-01 00:00:00
abstract::Cytokine research has yielded a range of products which have now reached the stage of clinical trials and a plethora of novel therapeutics may be expected. Recently, cytokine receptors have also become an area of intensive research. Preliminary results indicate that recombinant soluble receptors can interfere with the...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/0167-7799(90)90213-h
更新日期:1990-11-01 00:00:00