Abstract:
:Microbes are present at every crime scene and have been used as physical evidence for over a century. Advances in DNA sequencing and computational approaches have led to recent breakthroughs in the use of microbiome approaches for forensic science, particularly in the areas of estimating postmortem intervals (PMIs), locating clandestine graves, and obtaining soil and skin trace evidence. Low-cost, high-throughput technologies allow us to accumulate molecular data quickly and to apply sophisticated machine-learning algorithms, building generalizable predictive models that will be useful in the criminal justice system. In particular, integrating microbiome and metabolomic data has excellent potential to advance microbial forensics.
journal_name
Trends Biotechnoljournal_title
Trends in biotechnologyauthors
Metcalf JL,Xu ZZ,Bouslimani A,Dorrestein P,Carter DO,Knight Rdoi
10.1016/j.tibtech.2017.03.006subject
Has Abstractpub_date
2017-09-01 00:00:00pages
814-823issue
9eissn
0167-7799issn
1879-3096pii
S0167-7799(17)30055-0journal_volume
35pub_type
杂志文章,评审abstract::Attempts through somatic cell nuclear transfer to expand wild populations that have shrunk to critical numbers is a logical extension of the successful cloning of mammals. However, although the first mammal was cloned 10 years ago, nuclear reprogramming remains phenomenological, with abnormal gene expression and epige...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2007.03.007
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abstract::The realization of the important biomedical roles of polyunsaturated fatty acids has led to the development of methods for obtaining and manipulating polyunsaturated lipids. Enzyme-mediated reactions have demonstrated unique advantages over chemical approaches and commercial lipase- and phospholipase-catalysed process...
journal_title:Trends in biotechnology
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abstract::Vascularisation is key to developing large transplantable tissue constructs capable of providing therapeutic benefits. The vascularised tissue engineering chamber originates from surgical concepts in tissue prefabrication and microsurgery. It serves as an in vivo bioreactor in the form of a closed, protected space sur...
journal_title:Trends in biotechnology
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abstract::Rapid prototyping (RP) technologies are a set of manufacturing processes that can produce very complex structures directly from computer-aided design models without structure-specific tools or knowledge. These technologies might eventually enable the manufacture of human livers to create functional substitutes for tre...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2007.08.010
更新日期:2007-11-01 00:00:00
abstract::Phytoremediation holds promise for in situ treatment of heavy metal contaminated soils. Recently, the benefits of combining siderophore-producing bacteria (SPB) with plants for metal removal from contaminated soils have been demonstrated. Metal-resistant SPB play an important role in the successful survival and growth...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2009.12.002
更新日期:2010-03-01 00:00:00
abstract::The unicellular alga Dunaliella salina is the most interesting cell factory for the commercial production of beta-carotene because this species accumulates carotenoids to high concentrations. Nevertheless, little is known about the underlying mechanisms of carotenoid accumulation. Here, we review the regulatory mechan...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2008.07.002
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abstract::Building robust manufacturing processes from biological components is a task that is highly complex and requires sophisticated tools to describe processes, inputs, and measurements and administrate management of knowledge, data, and materials. We argue that for bioengineering to fully access biological potential, it w...
journal_title:Trends in biotechnology
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abstract::Predicting the future use of biocatalysts for the transformation of organic compounds--both natural substrates and other compounds--needs to take many factors into account. To date, relatively few biotransformations have been developed to the industrial scale, primarily because there has been little economic incentive...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/0167-7799(93)90079-O
更新日期:1993-11-01 00:00:00
abstract::We review published data on cell/gamete lyophilization. Most studies have utilized the same established protocols for cryopreservation (storage in liquid nitrogen) as for cell lyophilization (dehydration of frozen samples by water sublimation). Surveying natural lyoprotectants, we suggest trehalose and late embryogene...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2013.09.004
更新日期:2013-12-01 00:00:00
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journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2012.04.005
更新日期:2012-07-01 00:00:00
abstract::In vitro antibody generation technologies have now been available for two decades. Research reagents prepared via phage display are becoming available and several recent studies have demonstrated that these technologies are now sufficiently advanced to facilitate generation of a comprehensive renewable resource of ant...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2010.05.001
更新日期:2010-07-01 00:00:00
abstract::Life science in the 21st century is developing rapidly through the structural analysis of biomolecules, the completion of the human genome sequence and the analysis of transcriptomes. The mouse transcriptome has been comprehensively analyzed using a gene discovery approach to collect full-length cDNA (FL-cDNA) clones....
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2004.02.003
更新日期:2004-04-01 00:00:00
abstract::Room temperature ionic liquids are non-volatile, thermally stable and highly polar; they are also moderately hydrophilic solvents. Here, we discuss their use as reaction media for biocatalysis. Enzymes of widely diverging types are catalytically active in ionic liquids or aqueous biphasic ionic liquid systems. Lipases...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/S0167-7799(03)00008-8
更新日期:2003-03-01 00:00:00
abstract::The prevalence of antimicrobial-resistant pathogens has highlighted the urgent need for new drugs. Actinomycetes have been the most prominent sources of natural products for drug discovery and development. Advances in genomics have inspired several emerging strategies to reinvigorate the field of natural product disco...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2017.10.009
更新日期:2018-03-01 00:00:00
abstract::Biosensor technology can offer the livestock industry new types of monitoring and measuring devices of which the specificity, sensitivity, reproducibility, speed and ease of use exceed the current technology. Biosensors can be applied to the detection and identification of infectious diseases in livestock, contaminant...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/s0167-7799(01)01841-8
更新日期:2001-11-01 00:00:00
abstract::Luminescence has assumed an important role in analytical biochemistry and molecular biology as an extremely sensitive method for determining the concentration of specific ions and molecules. The luminescent system of the jellyfish Aequorea victoria consists of the photoprotein aequorin, which contains the molecule coe...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0167-7799(99)01379-7
更新日期:1999-12-01 00:00:00
abstract::Nanosilver (NS), comprising silver nanoparticles, is attracting interest for a range of biomedical applications owing to its potent antibacterial activity. It has recently been demonstrated that NS has useful anti-inflammatory effects and improves wound healing, which could be exploited in developing better dressings ...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2010.07.006
更新日期:2010-11-01 00:00:00
abstract::Many organisms have the ability to form spores, a remarkable phase in their life cycles. Compared with vegetative cells, spores have several advantages (e.g. resistance to toxic compounds, temperature, desiccation and radiation) making them well suited to various applications. The applications of spores that first spr...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/S0167-7799(03)00170-7
更新日期:2003-08-01 00:00:00
abstract::The field of regenerative medicine has blossomed in recent decades. However, the ultimate goal of tissue regeneration - replacing damaged or aged cells with healthy functioning cells - still faces a number of challenges. In particular, better understanding of the role of the cellular niche in shaping stem cell phenoty...
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abstract::The endothelium lining blood vessels expresses molecules that are restricted in their expression to a particular tissue or organ. These molecules are attractive targets for therapy and diagnosis because they allow agents to be delivered specifically to the blood vessels supplying the desired tissue. However, it is dif...
journal_title:Trends in biotechnology
pub_type: 杂志文章
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abstract::Pressure is a thermodynamic parameter whose unique effects on biological systems are increasingly being studied in a growing number of scientific fields. As such, the effects of high pressure are currently being investigated at different levels, ranging from proteins, enzymes and viruses to microorganisms, mammalian c...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2009.04.001
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abstract::Molecular systems in which fluorescence switches between 'on' and 'off' states when driven by chemical stimuli can be designed according to a few principles. The photochemical mechanisms examined are photoinduced electron transfer, internal charge transfer and excimer formation, with emphasis on the first category. Th...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
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更新日期:2001-01-01 00:00:00
abstract::Process analytical technology (PAT) for the manufacture of monoclonal antibodies (mAbs) is defined by an integrated set of advanced and automated methods that analyze the compositions and biophysical properties of cell culture fluids, cell-free product streams, and biotherapeutic molecules that are ultimately formulat...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
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更新日期:2020-10-01 00:00:00
abstract::Protein phosphorylation is a complex and highly dynamic process involved in numerous biological events. Abnormal phosphorylation is one of the underlying mechanisms for the development of cancer and metabolic disorders. The identification and absolute quantification of specific phospho-signatures can help elucidate pr...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2011.07.004
更新日期:2012-02-01 00:00:00
abstract::A switch from a petroleum-based to a biobased economy requires the capacity to produce both high-value low-volume and low-value high-volume products. Recent evidence supports the development of microalgae-based microbial cell factories with the objective of establishing environmentally sustainable manufacturing soluti...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
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更新日期:2020-06-01 00:00:00
abstract::Bacteriophages encode many distinct proteins for the successful infection of a bacterial host. Each protein plays a specific role in the phage replication cycle, from host recognition, through takeover of the host machinery, and up to cell lysis for progeny release. As the roles of these proteins are being revealed, m...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2018.04.006
更新日期:2018-09-01 00:00:00
abstract::Dual-use research, which results in knowledge that can be used for both good and ill, has become increasingly accessible in the internet age to both scientists and the general public. Here, we outline some major milestones for dual-use policy and present three vignettes that highlight contemporary dual-use issues in b...
journal_title:Trends in biotechnology
pub_type: 历史文章,杂志文章
doi:10.1016/j.tibtech.2018.07.016
更新日期:2018-12-01 00:00:00
abstract::The development of yeast artificial chromosome (YAC) cloning vectors capable of carrying several hundred kilobase-pairs of DNA insert has greatly facilitated the study of complex genomes, and the cloning of large genes as single fragments. In addition, the ability to manipulate YAC sequences by homologous recombinatio...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
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更新日期:1992-01-01 00:00:00
abstract::Step by step, the components of an artificial form of cellular life are being assembled by researchers. Lipid vesicles the size of small bacteria can be prepared and under certain conditions are able to grow and divide, then grow again. Polymerase enzymes encapsulated in the vesicles can synthesize RNA from externally...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2005.05.008
更新日期:2005-07-01 00:00:00
abstract::This article reports on the state-of-the-art technologies that sequence DNA using miniaturized devices. The article considers the miniaturization of existing technologies for sequencing DNA and the opportunities for cost reduction that 'on-chip' devices can deliver. The ability to construct nano-scale structures and p...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2007.07.001
更新日期:2007-09-01 00:00:00