Abstract:
:In vitro antibody generation technologies have now been available for two decades. Research reagents prepared via phage display are becoming available and several recent studies have demonstrated that these technologies are now sufficiently advanced to facilitate generation of a comprehensive renewable resource of antibodies for any protein encoded by the approximately 22,500 human protein-coding genes. Antibody selection in vitro offers properties not available in animal-based antibody generation methods. By adjusting the biochemical milieu during selection, it is possible to control the antigen conformation recognized, the antibody affinity or unwanted cross-reactivity. For larger-scale antibody generation projects, the handling, transport and storage logistics and bacterial production offer cost benefits. Because the DNA sequence encoding the antibody is available, modifications, such as site-specific in vivo biotinylation and multimerization, are only a cloning step away. This opinion article summarizes opportunities for the generation of antibodies for proteome research using in vitro technologies.
journal_name
Trends Biotechnoljournal_title
Trends in biotechnologyauthors
Dübel S,Stoevesandt O,Taussig MJ,Hust Mdoi
10.1016/j.tibtech.2010.05.001subject
Has Abstractpub_date
2010-07-01 00:00:00pages
333-9issue
7eissn
0167-7799issn
1879-3096pii
S0167-7799(10)00082-Xjournal_volume
28pub_type
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