Abstract：
:Clustered regularly interspaced short palindromic repeats (CRISPR)-mediated manipulation of genomic information is becoming more versatile by combining nuclease-deficient CRISPR systems with a wide variety of effectors including base-editing deaminases, transcriptional regulators, and epigenetic modifiers. The programmable binding ability of CRISPR systems is essential when the systems are employed as targeting domains to recruit the effectors to specific genomic loci. The discovery of a variety of Cas9 orthologs and engineered variants enables high-fidelity genome editing and a wider selection of genomic targets, and CRISPR-mediated deaminases enable more precise and predictable genome editing compared with CRISPR nuclease-based editing. Finally, combining transcriptional regulators with CRISPR systems can control expression of specific genes in a genome. Some applications and future challenges of CRISPR-derived tools are also discussed.
journal_name
Trends Biotechnoljournal_title
Trends in biotechnologyauthors
Mitsunobu H,Teramoto J,Nishida K,Kondo Adoi
10.1016/j.tibtech.2017.06.004subject
Has Abstractpub_date
2017-10-01 00:00:00pages
983-996issue
10eissn
0167-7799issn
1879-3096pii
S0167-7799(17)30132-4journal_volume
35pub_type
杂志文章,评审abstract：:Along with the development of metabolic engineering and synthetic biology tools, various microbes are being used to produce aromatic chemicals. In microbes, aromatics are mainly produced via a common important precursor, chorismate, in the shikimate pathway. Natural or non-natural aromatics have been produced by engin...
journal_title：Trends in biotechnology
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abstract：:Antimicrobial resistance (AMR) has developed into a huge threat to global health, and reducing it is an urgent priority for public health authorities. The importance of a healthy and balanced gut microbiome has been identified as a key protective factor against AMR development, but this can be significantly affected b...
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journal_title：Trends in biotechnology
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journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
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journal_title：Trends in biotechnology
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journal_title：Trends in biotechnology
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journal_title：Trends in biotechnology
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journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
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journal_title：Trends in biotechnology
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journal_title：Trends in biotechnology
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journal_title：Trends in biotechnology
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journal_title：Trends in biotechnology
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更新日期：2013-05-01 00:00:00
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journal_title：Trends in biotechnology
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journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
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journal_title：Trends in biotechnology
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journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
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更新日期：2020-01-01 00:00:00
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更新日期：2001-07-01 00:00:00
