Abstract:
:In recent years, entrapment of cells within spheres of Ca2+ alginate has become the most widely used technique for immobilizing living cells. This versatile method includes applications ranging from immobilization of living or dead cells in bioreactors, immobilization of plant protoplasts for micropropagation and immobilization of hybridoma cells for production of monoclonal antibodies, to entrapment of animal cells for implantation of artificial organs. This review evaluates the potential of this method on the basis of the current knowledge of structural and functional relationships in alginate gels.
journal_name
Trends Biotechnoljournal_title
Trends in biotechnologyauthors
Smidsrød O,Skjåk-Braek Gdoi
10.1016/0167-7799(90)90139-osubject
Has Abstractpub_date
1990-03-01 00:00:00pages
71-8issue
3eissn
0167-7799issn
1879-3096journal_volume
8pub_type
杂志文章,评审abstract::The discovery of targets that are sufficiently robust to yield marketable therapeutics is an enormous challenge. Through the years, several approaches have been used with varying degrees of success. These include target-independent screening of tumor-derived cell lines (disease-dependent), reductionist approaches to i...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s1471-1931(02)00199-4
更新日期:2002-12-01 00:00:00
abstract::This review summarizes synthetic chromosome construction in plants and speculates on how they might be applied to various uses in the future, as well as the possible impact for synthetic biology. The procedures to construct foundational platforms for synthetic chromosomes in plants are described, together with some ch...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2014.12.010
更新日期:2015-03-01 00:00:00
abstract::Step by step, the components of an artificial form of cellular life are being assembled by researchers. Lipid vesicles the size of small bacteria can be prepared and under certain conditions are able to grow and divide, then grow again. Polymerase enzymes encapsulated in the vesicles can synthesize RNA from externally...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2005.05.008
更新日期:2005-07-01 00:00:00
abstract::Feedstocks that deserve serious consideration for fuels and chemicals are sugarcane, corn, trees and algae. Commercialization of biomass refining is imminent but the wild claims of those who think that bioenergy can replace much of our dependence on foreign oil are appalling. It is naive to view biomass as the panacea...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2003.12.002
更新日期:2004-02-01 00:00:00
abstract::Selective delivery of lethal levels of drugs to tumors, without concomitant damage to normal tissues, is a major challenge in cancer chemotherapy. Prodrugs used in conjunction with enzyme-monoclonal antibody conjugates that can target tumors and convert prodrugs to their active drug forms in situ, offer exceptional pr...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/0167-7799(94)90122-8
更新日期:1994-06-01 00:00:00
abstract::Anywhere water is in the liquid state, bacteria will exist as biofilms, which are complex communities of cells that are cemented together. Although frequently associated with disease and biofouling, biofilms are also important for engineering applications, such as bioremediation, biocatalysis and microbial fuel cells....
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2010.11.001
更新日期:2011-02-01 00:00:00
abstract::The realization of the important biomedical roles of polyunsaturated fatty acids has led to the development of methods for obtaining and manipulating polyunsaturated lipids. Enzyme-mediated reactions have demonstrated unique advantages over chemical approaches and commercial lipase- and phospholipase-catalysed process...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/S0167-7799(97)01077-9
更新日期:1997-11-01 00:00:00
abstract::Many of the demands in the production of microalgae at a technical scale cannot presently be met by state-of-the-art cultivation technologies based on suspensions. Immobilized cultivation using porous substrate bioreactors (PSBRs) is characterized by a reduction of liquid reaction volumes by several orders of magnitud...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2016.06.004
更新日期:2017-02-01 00:00:00
abstract::Cyanobacteria promise to be an important industrial platform for the production of a variety of biobased products such as fuels, plastics, and isoprenoids. Recent advances in synthetic biology have resulted in various cyanobacterial strain improvements. Nevertheless, these new strains are still hampered by product inh...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2018.11.006
更新日期:2019-05-01 00:00:00
abstract::Europe has led many aspects of gene research and yet it has been unable to translate these discoveries into a globally dominant industrial sector. There are valid societal, political and financial reasons for its reluctance to deploy agricultural biotechnology but this reluctance might have unintended consequences. It...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0167-7799(00)01572-9
更新日期:2001-04-01 00:00:00
abstract::Reconstituted viral envelopes (RVEs) are formed by solubilizing intact virus in detergent and reassembling the envelope on removal of detergent. RVEs can be formed in the presence of agents that become encapsulated and can then be utilized in vitro and in vivo for drug delivery, cell destruction, transfer of membrane ...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/0167-7799(91)90184-J
更新日期:1991-02-01 00:00:00
abstract::Gastrointestinal cancers rank second in overall cancer-related deaths. Carotenoids, sulfated polysaccharides, and polyunsaturated fatty acids (PUFAs) from microalgae exhibit cancer chemopreventive features at different stages of carcinogenesis. For instance, sulfated polysaccharides bear a prophylactic potential via b...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2012.11.004
更新日期:2013-02-01 00:00:00
abstract::In Europe the commercialization of food derived from genetically modified plants has been slow because of the complex regulatory process and the concerns of consumers. Risk assessment is focused on potential adverse effects on humans and the environment, which could result from unintended effects of genetic modificati...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2006.01.009
更新日期:2006-03-01 00:00:00
abstract::Technology is a social practice that embodies the capacity of societies to transform themselves by creating and manipulating not only physical objects, but also symbols and cultural forms. It is an illusion that scientific and socioeconomic drivers are the sole elements determining the destiny of a technology. Althoug...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2007.08.012
更新日期:2007-12-01 00:00:00
abstract::Continued progress in cell-phone devices has made them powerful mobile computers, equipped with sophisticated, permanent physical sensors embedded as the default configuration. By contrast, the incorporation of permanent biosensors in cell-phone units has been prevented by the multivocal nature of the stimuli and the ...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2014.03.007
更新日期:2014-07-01 00:00:00
abstract::Success in synthetic biology and metabolic engineering is quickly becoming 'test' limited within the design-build-test cycle. Commonly used methods for high-throughput screening, including fluorescence-activated cell sorting (FACS) and microtiter plates, have intracellular product and throughput limitations. A growing...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2019.11.002
更新日期:2020-07-01 00:00:00
abstract::Soft biofunctional and biocompatible interfaces on solids designed by the deposition of ultrathin soft polymer films or supported membranes have numerous scientific and practical applications. These include the immobilization of glycolipids, membrane receptors and proteins to generate models of cell and tissue surface...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0167-7799(99)01412-2
更新日期:2000-02-01 00:00:00
abstract::Chimeric antigen receptor (CAR) technology and its application to regulatory T cells (Tregs) has garnered interest among researchers in the field of cell and gene therapy. Merging the benefits of CAR technology with Tregs offers a novel and promising therapeutic option for durable reshaping of undesired immune respons...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2019.12.009
更新日期:2020-10-01 00:00:00
abstract::Peptides possess several attractive features when compared to small molecule and protein therapeutics, such as high structural compatibility with target proteins, the ability to disrupt protein-protein interfaces, and small size. Efficient design of high-affinity peptide ligands via rational methods has been a major o...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2011.01.004
更新日期:2011-05-01 00:00:00
abstract::Toxic organic compounds (xenobiotics) pose serious environmental and health risks worldwide. Biological treatment of these materials is severely constrained by their toxic and inhibitory nature and great care is required with respect to the rate at which they are provided to cells. The use of a second, distinct, organ...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0167-7799(01)01789-9
更新日期:2001-11-01 00:00:00
abstract::The prevalence of antimicrobial-resistant pathogens has highlighted the urgent need for new drugs. Actinomycetes have been the most prominent sources of natural products for drug discovery and development. Advances in genomics have inspired several emerging strategies to reinvigorate the field of natural product disco...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2017.10.009
更新日期:2018-03-01 00:00:00
abstract::The analysis of biomolecules using microarrays and other biosensors has a significant role in molecular biotechnology, and will become even more important in the future as a versatile tool for research and diagnostics. For many applications, the synthetic DNA mimic peptide nucleic acid (PNA) could be advantageous as a...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2004.10.003
更新日期:2004-12-01 00:00:00
abstract::Taxol® (generic name paclitaxel) represents one of the most clinically valuable natural products known to mankind in the recent past. More than two decades have elapsed since the notable discovery of the first Taxol®-producing endophytic fungus, which was followed by a plethora of reports on other endophytes possessin...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2014.03.011
更新日期:2014-06-01 00:00:00
abstract::Higher fungi with greater than 70000 species are regarded as a rich source of various natural compounds including terpenoids, the production of which represents a wide range of interest in pharmaceutical and healthcare industries. This review summarizes the current knowledge of terpenoids synthesized by higher fungi, ...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2015.12.007
更新日期:2016-03-01 00:00:00
abstract::The best long-term prospects for the reduction in the number of deaths due to cancer lie with screening and prevention rather than with therapy of the advanced disease. Screening efficiency will be improved by the identification of individuals at inherited high risk of cancer. Understanding the mechanism of predisposi...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/0167-7799(90)90147-p
更新日期:1990-04-01 00:00:00
abstract::Novel therapeutic strategies that promote wound healing seek to mimic the response of the body to wounding, to regenerate rather than repair injured tissues. Many synthetic or natural biomaterials have been developed for this purpose and are used to deliver wound therapeutics in a controlled manner that prevents unwan...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2006.05.001
更新日期:2006-07-01 00:00:00
abstract::The drive for industrial sustainability has pushed biosurfactants to the top of the agenda of many companies. Biosurfactants offer the possibility of replacing chemical surfactants, produced from nonrenewable resources, with alternatives produced from cheap renewable feedstocks. Biosurfactants are also attractive beca...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2012.07.003
更新日期:2012-11-01 00:00:00
abstract::Short, self-assembling peptides form a variety of stable nanostructures used for the rational design of functional devices. Peptides serve as organic templates for conjugating biorecognition elements, and assembling ordered nanoparticle arrays and hybrid supramolecular structures. We are witnessing the emergence of a ...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2011.11.001
更新日期:2012-03-01 00:00:00
abstract::Although many microorganisms have been used for the bioindustrial generation of valuable metabolites, the productive potential of cyanobacterial species has remained largely unexplored. Cyanobacteria possess several advantages as organisms for bioindustrial processes, including simple input requirements, tolerance of ...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2010.12.003
更新日期:2011-02-01 00:00:00
abstract::Despite the wealth of research on stem-cell biology and therapy, there have been few advances in the ex vivo expansion of adult stem cells. Fetal bovine serum, which can elicit an immune reaction in hosts receiving cellular products, remains essential to the culture methods. Recent work by Prockop et al. suggests that...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2004.05.006
更新日期:2004-08-01 00:00:00