Abstract:
:Life science in the 21st century is developing rapidly through the structural analysis of biomolecules, the completion of the human genome sequence and the analysis of transcriptomes. The mouse transcriptome has been comprehensively analyzed using a gene discovery approach to collect full-length cDNA (FL-cDNA) clones. The framework of the transcriptome was then mapped out by an international Functional ANnoTation Of Mouse cDNA (FANTOM) effort, and a significant new population of noncoding transcripts was discovered. The geographical analogy of a second "RNA continent," separate from the "continent" of expressed proteins, aids the visualization of this concept. An unexpected number of variations was discovered in the mouse transcriptome. The animal transcriptome has evolved to produce several transcripts and proteins from a single "transcriptional unit". Transcriptome analysis has given rise to the FL-cDNA database and to the 60 770 FANTOM FL-cDNA clone set, and the DNABook was developed as an easier way to distribute these clones. In conjunction with genome sequence databases, transcriptome databases and clone banks will be platforms for developing advanced databases of gene function (e.g. the Genome Function Database). This will enable life science to make rapid progress towards understanding life as a system of molecules.
journal_name
Trends Biotechnoljournal_title
Trends in biotechnologyauthors
Hayashizaki Y,Kanamori Mdoi
10.1016/j.tibtech.2004.02.003subject
Has Abstractpub_date
2004-04-01 00:00:00pages
161-7issue
4eissn
0167-7799issn
1879-3096pii
S0167-7799(04)00049-6journal_volume
22pub_type
杂志文章,评审abstract::There is a need for techniques that ensure antibiotic removal in anaerobic digesters for robust methane production. In this article, we discuss recent strategies for enhanced antibiotic removal from swine wastewater and offer insights on anaerobic digestion (AD) process design for improved antibiotic removal. ...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2020.07.002
更新日期:2021-01-01 00:00:00
abstract::Opinions on what synthetic biology actually is range from a natural extension of genetic engineering to a new manufacturing paradigm. It offers, for the first time in the life sciences, rational design and engineering standardisation. It could address problems across a broad spectrum of human concerns, including energ...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2013.01.011
更新日期:2013-05-01 00:00:00
abstract::In the future, biomass will continue to emerge as a viable source of chemicals. The development of new industries that utilize bio-renewables provides opportunities for innovation. For example, bio- and chemo-catalysts can be combined in 'one pot' to prepare chemicals of commercial value. This has been demonstrated us...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2011.01.005
更新日期:2011-05-01 00:00:00
abstract::Molecularly imprinted polymers (MIPs) are prepared by creating a three-dimensional polymeric matrix around a template molecule. After the matrix is removed, complementary cavities with respect to shape and functional groups remain. MIPs have been produced for applications in in vitro diagnostics, therapeutics and sepa...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2010.08.006
更新日期:2010-12-01 00:00:00
abstract::Peptide nucleic acids (PNAs) are novel DNA mimics in which the sugar-phosphate backbone has been replaced with a backbone based on amino acids. PNAs exhibit sequence-specific binding to DNA and RNA with higher affinities and specificities than unmodified DNA. They are resistant to nuclease and protease attack in serum...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/0167-7799(93)90097-S
更新日期:1993-09-01 00:00:00
abstract::A recent publication by Michael Longaker and colleagues represents a landmark for the use of adipose tissue as a source of cells for tissue regeneration. The authors investigated the ability of adipose tissue-derived cells (ADCs) to regenerate critical size calvarial (superior portion of the skull) defects in mice by ...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2004.12.003
更新日期:2005-02-01 00:00:00
abstract::Precise control of transgene expression is pivotal to the engineering of plants with increased disease resistance. Many early attempts to boost disease resistance used constitutive overexpression of defence components but frequently this resulted in poor quality plants. It is now clear that the extensive cellular repr...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2005.04.009
更新日期:2005-06-01 00:00:00
abstract::The best long-term prospects for the reduction in the number of deaths due to cancer lie with screening and prevention rather than with therapy of the advanced disease. Screening efficiency will be improved by the identification of individuals at inherited high risk of cancer. Understanding the mechanism of predisposi...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/0167-7799(90)90147-p
更新日期:1990-04-01 00:00:00
abstract::Microfluidic devices offer automation and high-throughput screening, and operate at low volumes of consumables. Although microfluidics has the potential to reduce turnaround times and costs for analytical devices, particularly in medical, veterinary, and environmental sciences, this enabling technology has had limited...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2014.04.010
更新日期:2014-07-01 00:00:00
abstract::Lyases catalyse the cleavage of C-C, C-N, C-O and other bonds by elimination to produce double bonds or, conversely, catalyse the addition of groups to double bonds. These enzymes do not require cofactor recycling, show an absolute stereospecificity and can give a theoretical yield of 100%, compared with only 50% for ...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/0167-7799(94)90112-0
更新日期:1994-03-01 00:00:00
abstract::There are many paths toward effective microbial inoculants for agriculture. Considering what is practical for the present day technological and farming landscape should not limit our creativity in developing innovative technologies. However, factors including production costs, practicality of implementation, and techn...
journal_title:Trends in biotechnology
pub_type: 评论,信件
doi:10.1016/j.tibtech.2019.02.009
更新日期:2019-06-01 00:00:00
abstract::Bacteriophages encode many distinct proteins for the successful infection of a bacterial host. Each protein plays a specific role in the phage replication cycle, from host recognition, through takeover of the host machinery, and up to cell lysis for progeny release. As the roles of these proteins are being revealed, m...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2018.04.006
更新日期:2018-09-01 00:00:00
abstract::Intravital imaging enables to study dynamic tumour-stroma interactions within primary and metastatic sites, including the lung. The combination of optical windows with specific molecular probes targeting the tumour microenvironment will provide new insights into prometastatic stromal interactions and lead to novel the...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2016.05.001
更新日期:2017-01-01 00:00:00
abstract::Technology is a social practice that embodies the capacity of societies to transform themselves by creating and manipulating not only physical objects, but also symbols and cultural forms. It is an illusion that scientific and socioeconomic drivers are the sole elements determining the destiny of a technology. Althoug...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2007.08.012
更新日期:2007-12-01 00:00:00
abstract::Bovine mastitis, the most significant disease of dairy herds, has huge effects on farm economics due to reduction in milk production and treatment costs. Traditionally, methods of detection have included estimation of somatic cell counts, an indication of inflammation, measurement of biomarkers associated with the ons...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2009.05.004
更新日期:2009-08-01 00:00:00
abstract::Molecular systems in which fluorescence switches between 'on' and 'off' states when driven by chemical stimuli can be designed according to a few principles. The photochemical mechanisms examined are photoinduced electron transfer, internal charge transfer and excimer formation, with emphasis on the first category. Th...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0167-7799(00)01513-4
更新日期:2001-01-01 00:00:00
abstract::A major goal of material science is to produce hierarchical materials that are ordered on all length scales, from the molecular (1-100 A) via the nano (10-100 nm) to the meso (1-100 microm). In these materials, the larger-scale properties can be controlled by choosing molecular characteristics. Methods developed to pr...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/s0167-7799(00)01482-7
更新日期:2000-09-01 00:00:00
abstract::The ascomycete Trichoderma reesei is one of the most well studied cellulolytic microorganisms. This fungus is widely used in the biotechnology industry, mainly in the production of biofuels. Due to its importance, its genome was sequenced in 2008, opening new avenues to study this microorganism. In this 'post-genomic'...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2016.06.003
更新日期:2016-12-01 00:00:00
abstract::Despite considerable progress in the development of cell culture techniques, including the development of the serum- and protein-free media that now routinely support hybridoma and mammalian cell growth, fetal bovine serum (FBS) supplemented media are still commonly used: a practice that raises ethical, scientific and...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2006.01.001
更新日期:2006-03-01 00:00:00
abstract::Modern biocatalysis is developing new and precise tools to improve a wide range of production processes, which reduce energy and raw material consumption and generate less waste and toxic side-products. Biocatalysis is also achieving new advances in environmental fields, from enzymatic bioremediation to the synthesis ...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2006.04.002
更新日期:2006-06-01 00:00:00
abstract::Spheroids provide a 3D environment with intensive cell-cell contacts. As a result of their excellent regenerative properties and rapid progress in their high-throughput production, spheroids are increasingly suggested as building blocks for tissue engineering. In this review, we focus on innovative biotechnological ap...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2016.08.004
更新日期:2017-02-01 00:00:00
abstract::Atmospheric pressure gas plasmas are emerging as a promising treatment in cancer that can supplement the existing set of treatment modalities and, when combined with other therapies, enhance their selectivity and efficacy against resistant cancers. With further optimisation in production and administration of plasma t...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2018.06.010
更新日期:2018-11-01 00:00:00
abstract::Europe has led many aspects of gene research and yet it has been unable to translate these discoveries into a globally dominant industrial sector. There are valid societal, political and financial reasons for its reluctance to deploy agricultural biotechnology but this reluctance might have unintended consequences. It...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0167-7799(00)01572-9
更新日期:2001-04-01 00:00:00
abstract::Discussions on research quality and reproducibility are appearing in the pages of scientific journals with heightened significance and gaining media attention. Many institutions have developed guidelines to address the topic of quality in basic research, but questions remain about how best to implement and monitor com...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2013.06.007
更新日期:2013-10-01 00:00:00
abstract::Allergenicity prediction is one of the most challenging aspects in the safety assessment of foods derived from either biotechnology or novel food proteins. Here we present a bottom-up strategy that defines a priori the specific risk assessment (RA) needs based on a database appropriately built for such purposes. ...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2020.05.011
更新日期:2021-01-01 00:00:00
abstract::Hydrophobic charge induction chromatography using 4-mercapto-ethyl-pyridine as the ligand is an effective method for the separation of antibodies from a variety of feedstocks. Antibodies are adsorbed in physiological conditions without preliminary concentration. Desorption occurs when the pH is lowered, thus inducing ...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0167-7799(02)01980-7
更新日期:2002-08-01 00:00:00
abstract::Type 1 Diabetes (T1D) is an autoimmune disease resulting from the destruction of pancreatic insulin-producing beta cells that most frequently occurs in genetically predisposed children. Recent observations illustrating the regenerative capability of the endocrine pancreas in addition to advances in stem cell and gene ...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2006.08.007
更新日期:2006-11-01 00:00:00
abstract::Advances in our capacity to design and use novel strategies for achieving inducible gene expression will improve our ability to define gene function. An extremely efficient system designed by nature -- that of the regulatable phytochrome system in plants -- has provided the basis for developing a novel inducible gene ...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/s0167-7799(02)00022-7
更新日期:2003-02-01 00:00:00
abstract::Building robust manufacturing processes from biological components is a task that is highly complex and requires sophisticated tools to describe processes, inputs, and measurements and administrate management of knowledge, data, and materials. We argue that for bioengineering to fully access biological potential, it w...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2015.11.006
更新日期:2016-03-01 00:00:00
abstract::Adsorption in stable expanded beds enables proteins to be recovered directly from particulate-containing feedstocks, such as fermentation broths and preparations of disrupted cells, without the need for prior removal of the suspended solids, which would normally result in the blockage of packed beds. The adoption of t...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/0167-7799(94)90046-9
更新日期:1994-08-01 00:00:00