The growing importance of fat in regenerative medicine.

abstract：:Attempts through somatic cell nuclear transfer to expand wild populations that have shrunk to critical numbers is a logical extension of the successful cloning of mammals. However, although the first mammal was cloned 10 years ago, nuclear reprogramming remains phenomenological, with abnormal gene expression and epige...

journal_title：Trends in biotechnology

authors： Loi P,Galli C,Ptak G

更新日期：2007-05-01 00:00:00

abstract：:Molecular systems in which fluorescence switches between 'on' and 'off' states when driven by chemical stimuli can be designed according to a few principles. The photochemical mechanisms examined are photoinduced electron transfer, internal charge transfer and excimer formation, with emphasis on the first category. Th...

journal_title：Trends in biotechnology

authors： de Silva AP,Fox DB,Moody TS,Weir SM

更新日期：2001-01-01 00:00:00

abstract：:The redundancy of natural product biosynthesis in microbes poses a practical challenge for discovering new antimicrobial compounds from bacteria. The recent application of clustered regularly interspaced short palindromic repeats (CRISPR) technology by Culp et al. to inactivate the production of abundant antibiotics g...

journal_title：Trends in biotechnology

authors： Timmermans ML,Ross AC

更新日期：2020-01-01 00:00:00

abstract：:Techniques such as positron-emission tomography, single-photon-emission computed tomography, functional magnetic-resonance imaging and magnetoencephalography permit the observation of biological processes in the brain in a noninvasive manner. They have yielded new insights into the biological interrelations of sensory...

journal_title：Trends in biotechnology

authors： Müller-Gärtner HW

更新日期：1998-03-01 00:00:00

abstract：:Adsorption in stable expanded beds enables proteins to be recovered directly from particulate-containing feedstocks, such as fermentation broths and preparations of disrupted cells, without the need for prior removal of the suspended solids, which would normally result in the blockage of packed beds. The adoption of t...

journal_title：Trends in biotechnology

authors： Chase HA

更新日期：1994-08-01 00:00:00

abstract：:Advances in high-throughput DNA-sequencing techniques have given us the unprecedented ability to rapidly determine the nucleotide sequences of entire bacterial genomes. The application of these methods to the genomes of microbial pathogens, combined with efficient analytical tools and genome-scale approaches for study...

journal_title：Trends in biotechnology

authors： Smith DR

更新日期：1996-08-01 00:00:00

abstract：:The extensive use of chemical insecticides for insect pest management has resulted in insecticide resistance now being recorded in >500 species of insects and mites. Although gut-active toxins such as those derived from Bacillus thuringiensis (Bt) have been successfully used for insect pest management, a diverse range...

journal_title：Trends in biotechnology

authors： Bonning BC,Chougule NP

更新日期：2014-02-01 00:00:00

abstract：:Europe has led many aspects of gene research and yet it has been unable to translate these discoveries into a globally dominant industrial sector. There are valid societal, political and financial reasons for its reluctance to deploy agricultural biotechnology but this reluctance might have unintended consequences. It...

journal_title：Trends in biotechnology

authors： Enriquez J

更新日期：2001-04-01 00:00:00

abstract：:The glycosylation profile of a recombinant glycoprotein can not only significantly affect its therapeutic profile, but is also extremely sensitive to cell-culture and purification conditions. To define glycosylation patterns and to ensure consistency of recombinant glycoproteins among different preparations requires h...

journal_title：Trends in biotechnology

authors： Parekh RB,Patel TP

更新日期：1992-08-01 00:00:00

abstract：:Brain-machine interfaces (BMIs) enable humans to interact with devices by modulating their brain signals. Despite impressive technological advancements, several obstacles remain. The most commonly used BMI control signals are derived from the brain areas involved in primary sensory- or motor-related processing. Howeve...

journal_title：Trends in biotechnology

authors： Min BK,Chavarriaga R,Millán JDR

更新日期：2017-07-01 00:00:00

abstract：:Process analytical technology (PAT) for the manufacture of monoclonal antibodies (mAbs) is defined by an integrated set of advanced and automated methods that analyze the compositions and biophysical properties of cell culture fluids, cell-free product streams, and biotherapeutic molecules that are ultimately formulat...

journal_title：Trends in biotechnology

authors： Maruthamuthu MK,Rudge SR,Ardekani AM,Ladisch MR,Verma MS

更新日期：2020-10-01 00:00:00

abstract：:The global movement of people and goods has increased the risk of biosecurity threats and their potential to incur large economic, social, and environmental costs. Conventional manual biosecurity surveillance methods are limited by their scalability in space and time. This article focuses on autonomous surveillance sy...

journal_title：Trends in biotechnology

authors： Jurdak R,Elfes A,Kusy B,Tews A,Hu W,Hernandez E,Kottege N,Sikka P

更新日期：2015-04-01 00:00:00

abstract：:Carotenoids are micronutrients important for human health. The continuous improvements in clustered regularly interspaced short palindromic repeats (CRISPR)-based genome-editing techniques make rapid, DNA/transgene-free and targeted multiplex genetic modification a reality, thus promising to accelerate the breeding an...

journal_title：Trends in biotechnology

authors： Zheng X,Kuijer HNJ,Al-Babili S

更新日期：2020-12-29 00:00:00

abstract：:Innovative fermentation processes are necessary for the cost-effective production of bulk chemicals from renewable resources. Current microbial processes are either anaerobic processes, with high yield and productivity, or less-efficient aerobic processes. Oxygen utilization plays an important role in energy generatio...

journal_title：Trends in biotechnology

authors： Weusthuis RA,Lamot I,van der Oost J,Sanders JP

更新日期：2011-04-01 00:00:00

abstract：:Nanomaterials have been developed for many biomedical applications, including medical imaging, drug delivery, and antimicrobial coatings. Intriguingly, nanoparticles can display 'enzyme-like' activity and have been explored as alternatives to natural enzymes in several industrial and energy-related applications. Recen...

journal_title：Trends in biotechnology

authors： Cormode DP,Gao L,Koo H

更新日期：2018-01-01 00:00:00

abstract：:Transgenic plants that express foreign proteins with industrial or pharmaceutical value represent an economical alternative to fermentation-based production systems. Specific vaccines have been produced in plants as a result of the transient or stable expression of foreign genes. It has recently been shown that genes ...

journal_title：Trends in biotechnology

authors： Mason HS,Arntzen CJ

更新日期：1995-09-01 00:00:00

abstract：:The use of antibodies or antibody fragments for targeting tumors (either for tumor imaging or as carriers for drugs or toxins), has encountered problems of clearance, and non-specific or inefficient binding in clinical trials. A novel approach, linking two antibody variable fragments (Fvs), with a short peptide to gen...

journal_title：Trends in biotechnology

authors： Bird RE,Walker BW

更新日期：1991-04-01 00:00:00

abstract：:In vitro antibody generation technologies have now been available for two decades. Research reagents prepared via phage display are becoming available and several recent studies have demonstrated that these technologies are now sufficiently advanced to facilitate generation of a comprehensive renewable resource of ant...

journal_title：Trends in biotechnology

authors： Dübel S,Stoevesandt O,Taussig MJ,Hust M

更新日期：2010-07-01 00:00:00

abstract：:The ascomycete Trichoderma reesei is one of the most well studied cellulolytic microorganisms. This fungus is widely used in the biotechnology industry, mainly in the production of biofuels. Due to its importance, its genome was sequenced in 2008, opening new avenues to study this microorganism. In this 'post-genomic'...

journal_title：Trends in biotechnology

authors： Gupta VK,Steindorff AS,de Paula RG,Silva-Rocha R,Mach-Aigner AR,Mach RL,Silva RN

更新日期：2016-12-01 00:00:00

abstract：:Tremendous advances in our understanding of the human immunodeficiency viruses (HIVs) have been made through the use of animal models. However, there are limitations inherent in many of the current models that use either nonhuman primates, or viruses other than HIV-1. Researchers continue to search for improved models...

journal_title：Trends in biotechnology

authors： Lewis AD,Johnson PR

更新日期：1995-04-01 00:00:00

abstract：:Feedstocks that deserve serious consideration for fuels and chemicals are sugarcane, corn, trees and algae. Commercialization of biomass refining is imminent but the wild claims of those who think that bioenergy can replace much of our dependence on foreign oil are appalling. It is naive to view biomass as the panacea...

journal_title：Trends in biotechnology

authors： Bungay HR

更新日期：2004-02-01 00:00:00

abstract：:Bovine mastitis, the most significant disease of dairy herds, has huge effects on farm economics due to reduction in milk production and treatment costs. Traditionally, methods of detection have included estimation of somatic cell counts, an indication of inflammation, measurement of biomarkers associated with the ons...

journal_title：Trends in biotechnology

authors： Viguier C,Arora S,Gilmartin N,Welbeck K,O'Kennedy R

更新日期：2009-08-01 00:00:00

abstract：:Recent advances in the extraction and purification of decellularized extracellular matrix (dECM) obtained from healthy or malignant tissues open new avenues for engineering physiomimetic 3D in vitro tumor models, which closely recapitulate key biomolecular hallmarks and the dynamic cancer cell-ECM interactions in the ...

journal_title：Trends in biotechnology

authors： Ferreira LP,Gaspar VM,Mano JF

更新日期：2020-12-01 00:00:00

abstract：:The baculovirus expression system has been used extensively for the expression of recombinant proteins in insect cells. Recently, recombinant baculovirus vectors engineered to contain mammalian cell-active promoter elements, have been used successfully for transient and stable gene delivery in a broad spectrum of prim...

journal_title：Trends in biotechnology

authors： Kost TA,Condreay JP

更新日期：2002-04-01 00:00:00

abstract：:Many pathogens have the ability to vary the antigenic composition of surface-associated antigens. Often, this variation is mediated by the regulation of gene expression. By varying its antigenicity, the pathogen is able to avoid host immune responses more efficiently; however, this makes the design of vaccines against...

journal_title：Trends in biotechnology

authors： Maskell D,Frankel G,Dougan G

更新日期：1993-12-01 00:00:00

abstract：:Gene expression noise is a significant source of phenotypic heterogeneity in otherwise identical populations of cells. Phenotypic heterogeneity can cause reversible drug resistance in diseased cells, and thus a better understanding of its origins might improve treatment strategies. In eukaryotes, data strongly suggest...

journal_title：Trends in biotechnology

authors： Miller-Jensen K,Dey SS,Schaffer DV,Arkin AP

更新日期：2011-10-01 00:00:00

abstract：:The availability of the human genome sequence has enabled the exploration and exploitation of the human genome and proteome to begin. Research has now focussed on the annotation of the genome and in particular of the proteome. With expert annotation extracted from the literature by biologists as the foundation, it has...

journal_title：Trends in biotechnology

authors： O'Donovan C,Apweiler R,Bairoch A

更新日期：2001-05-01 00:00:00

abstract：:We have isolated several microorganisms that are adapted to living in the extremes of the deep-sea environment. They include barophilic bacteria, which are able to grow at high hydrostatic pressure, but that are unable to grow at atmospheric pressure, and organic-solvent-tolerant bacteria, which are able to grow in th...

journal_title：Trends in biotechnology

authors： Kato C,Inoue A,Horikoshi K

更新日期：1996-01-01 00:00:00

abstract：:One of the goals of synthetic biology is to build regulatory circuits that control cell behavior, for both basic research purposes and biomedical applications. The ability to build transcriptional regulatory devices depends on the availability of programmable, sequence-specific, and effective synthetic transcription f...

journal_title：Trends in biotechnology

authors： Jusiak B,Cleto S,Perez-Piñera P,Lu TK

更新日期：2016-07-01 00:00:00

abstract：:Mutasynthesis couples the power of chemical synthesis with molecular biology to generate derivatives of medicinally valuable, natural products. Recently, this technique has been exploited by Cambridge-based biotech company Biotica Technology Ltd, and their collaborators, to generate promising new variants of the polyk...

journal_title：Trends in biotechnology

authors： Weissman KJ

更新日期：2007-04-01 00:00:00