Abstract：
:The glycosylation profile of a recombinant glycoprotein can not only significantly affect its therapeutic profile, but is also extremely sensitive to cell-culture and purification conditions. To define glycosylation patterns and to ensure consistency of recombinant glycoproteins among different preparations requires highly sensitive and reproducible analytical methods that can be used routinely. New strategies and instrumentation are being developed which should allow such analysis to be largely automated.
journal_name
Trends Biotechnoljournal_title
Trends in biotechnologyauthors
Parekh RB,Patel TPdoi
10.1016/0167-7799(92)90244-psubject
Has Abstractpub_date
1992-08-01 00:00:00pages
276-80issue
8eissn
0167-7799issn
1879-3096journal_volume
10pub_type
杂志文章,评审abstract：:In vitro selection from molecular libraries has rapidly come of age as a protein-engineering tool. Dramatic increases in protein affinity can be engineered using phage-display libraries, and specific antibodies can be selected directly from a single 'naïve' library of their genes. Repertoires of small molecules are a ...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0167-7799(94)90079-5
更新日期：1994-05-01 00:00:00
abstract：:Acidity is a biomarker of cancer that is not subject to the blunting clonal selection effects that reduce the efficacy of other biomarker technologies, such as antibody targeting. The pH (low) insertion peptides (pHLIP®s) provide new opportunities for targeting acidic tissues. Through the physical mechanism of membran...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2017.03.014
更新日期：2017-07-01 00:00:00
abstract：:In 2006, the group of Dr C.M. Lieber pioneered the field of nanowire sensors by fabricating devices for the ultra-sensitive label-free detection of biological macromolecules. Since then, nanowire sensors have demonstrated their ability to detect cancer-associated analytes in peripheral blood, tumor tissue, and the exh...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2018.07.014
更新日期：2019-01-01 00:00:00
abstract：:Bioremediation differs from other industrial biotechnologies in that, although bioremediation contractors must profit from the activity, the primary driver is regulatory compliance rather than manufacturing profit. It is an attractive technology in the context of a bioeconomy but currently has limitations at the field...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2013.01.015
更新日期：2013-06-01 00:00:00
abstract：:Phytoremediation holds promise for in situ treatment of heavy metal contaminated soils. Recently, the benefits of combining siderophore-producing bacteria (SPB) with plants for metal removal from contaminated soils have been demonstrated. Metal-resistant SPB play an important role in the successful survival and growth...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2009.12.002
更新日期：2010-03-01 00:00:00
abstract：:Type 1 Diabetes (T1D) is an autoimmune disease resulting from the destruction of pancreatic insulin-producing beta cells that most frequently occurs in genetically predisposed children. Recent observations illustrating the regenerative capability of the endocrine pancreas in addition to advances in stem cell and gene ...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2006.08.007
更新日期：2006-11-01 00:00:00
abstract：:The unicellular alga Dunaliella salina is the most interesting cell factory for the commercial production of beta-carotene because this species accumulates carotenoids to high concentrations. Nevertheless, little is known about the underlying mechanisms of carotenoid accumulation. Here, we review the regulatory mechan...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2008.07.002
更新日期：2008-11-01 00:00:00
abstract：:The mapping of the human genome was completed earlier this year and efforts are underway to understand the role of gene products (i.e. proteins) in biological pathways and human disease and to exploit their functional roles to derive protein therapeutics and protein-based drugs. A key component to the next revolution ...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0167-7799(01)01666-3
更新日期：2001-07-01 00:00:00
abstract：:Peptides possess several attractive features when compared to small molecule and protein therapeutics, such as high structural compatibility with target proteins, the ability to disrupt protein-protein interfaces, and small size. Efficient design of high-affinity peptide ligands via rational methods has been a major o...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2011.01.004
更新日期：2011-05-01 00:00:00
abstract：:Peptide nucleic acids (PNAs) are novel DNA mimics in which the sugar-phosphate backbone has been replaced with a backbone based on amino acids. PNAs exhibit sequence-specific binding to DNA and RNA with higher affinities and specificities than unmodified DNA. They are resistant to nuclease and protease attack in serum...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/0167-7799(93)90097-S
更新日期：1993-09-01 00:00:00
abstract：:A recent publication by Michael Longaker and colleagues represents a landmark for the use of adipose tissue as a source of cells for tissue regeneration. The authors investigated the ability of adipose tissue-derived cells (ADCs) to regenerate critical size calvarial (superior portion of the skull) defects in mice by ...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2004.12.003
更新日期：2005-02-01 00:00:00
abstract：:Wheat is one of the major staple food crops grown worldwide; however, productivity in cereal crops has not kept pace with the world population growth. A significant increase in wheat production (>40% by 2020) is needed simply to keep up with the growing demand. This increase is unlikely to be achieved by conventional ...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2006.04.008
更新日期：2006-07-01 00:00:00
abstract：:Atmospheric pressure gas plasmas are emerging as a promising treatment in cancer that can supplement the existing set of treatment modalities and, when combined with other therapies, enhance their selectivity and efficacy against resistant cancers. With further optimisation in production and administration of plasma t...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2018.06.010
更新日期：2018-11-01 00:00:00
abstract：:Precise control of transgene expression is pivotal to the engineering of plants with increased disease resistance. Many early attempts to boost disease resistance used constitutive overexpression of defence components but frequently this resulted in poor quality plants. It is now clear that the extensive cellular repr...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2005.04.009
更新日期：2005-06-01 00:00:00
abstract：:Cultured mammalian cells are the main workhorses for producing biologics. The performance of these cell culture processes, in terms of both productivity and product quality attributes, is significantly influenced by cellular metabolism. Glucose is the major carbon source for cellular biosynthesis and energy generation...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2016.04.012
更新日期：2016-08-01 00:00:00
abstract：:There are many aims associated with the optimization of fermentation processes. Optimization is expected to increase the yield of the final product but the process must be compliant with good manufacturing practices, the available equipment and the expected final scale of operation. Dealing with genetically modified m...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/s0167-7799(02)01913-3
更新日期：2002-03-01 00:00:00
abstract：:Flow cytometry is a rapid method for the quantitative measurement of light scattering and fluorescent properties of cells. Although this technique has been widely applied to biomedical and environmental studies, its potential as a tool in ecotoxicological studies has not yet been fully exploited. This article describe...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0167-7799(01)01924-2
更新日期：2002-04-01 00:00:00
abstract：:Wearable sensors have garnered considerable recent interest owing to their tremendous promise for a plethora of applications. Yet the absence of reliable non-invasive chemical sensors has greatly hindered progress in the area of on-body sensing. Electrochemical sensors offer considerable promise as wearable chemical s...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2014.04.005
更新日期：2014-07-01 00:00:00
abstract：:Metabolomics embraces several strategies that aim to quantify cell metabolites in order to increase our understanding of how metabolite levels and interactions influence phenotypes. Metabolic footprinting represents a niche within metabolomics, because it focuses on the analysis of extracellular metabolites. Although ...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2008.05.008
更新日期：2008-09-01 00:00:00
abstract：:Higher fungi with greater than 70000 species are regarded as a rich source of various natural compounds including terpenoids, the production of which represents a wide range of interest in pharmaceutical and healthcare industries. This review summarizes the current knowledge of terpenoids synthesized by higher fungi, ...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2015.12.007
更新日期：2016-03-01 00:00:00
abstract：:In the wake of public distrust regarding biotechnology, it has been suggested that the debate should be moved "upstream", whereby the public help to set research priorities. Although many scientists see this as an illogical reaction to a loss of faith in science, we argue that the boundaries between science and its te...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2005.12.007
更新日期：2006-02-01 00:00:00
abstract：:Regenerative medicine approaches based on decellularized extracellular matrix (ECM) scaffolds and tissues are rapidly expanding. The rationale for using ECM as a natural biomaterial is the presence of bioactive molecules that drive tissue homeostasis and regeneration. Moreover, appropriately prepared ECM is biodegrada...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2012.12.004
更新日期：2013-03-01 00:00:00
abstract：:Techniques such as positron-emission tomography, single-photon-emission computed tomography, functional magnetic-resonance imaging and magnetoencephalography permit the observation of biological processes in the brain in a noninvasive manner. They have yielded new insights into the biological interrelations of sensory...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/S0167-7799(97)01163-3
更新日期：1998-03-01 00:00:00
abstract：:Cell-surface display allows peptides and proteins to be displayed on the surface of microbial cells by fusing them with the anchoring motifs. The protein to be displayed - the passenger protein - can be fused to an anchoring motif - the carrier protein - by N-terminal fusion, C-terminal fusion or sandwich fusion. The ...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0167-7799(02)00006-9
更新日期：2003-01-01 00:00:00
abstract：:The general acceptance of bioremediation technology as an environmentally sound and economic treatment for hazardous waste requires the demonstration of its efficacy, reliability and predictability, as well as its advantages over conventional treatments. An effective monitoring design includes protocols for treatment-...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/0167-7799(93)90156-4
更新日期：1993-08-01 00:00:00
abstract：:The discovery of targets that are sufficiently robust to yield marketable therapeutics is an enormous challenge. Through the years, several approaches have been used with varying degrees of success. These include target-independent screening of tumor-derived cell lines (disease-dependent), reductionist approaches to i...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s1471-1931(02)00199-4
更新日期：2002-12-01 00:00:00
abstract：:Small molecules are defined as low molecular weight organic compounds (typically <1000 Da), which could be either natural or artificial. Because established imaging methods are not able to selectively detect the positions, concentrations and structures of small molecules in biological samples, new methods have been de...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2010.05.005
更新日期：2010-08-01 00:00:00
abstract：:Cyclodextrins, macrocyclic carbohydrates with apolar internal cavities, can form complexes with, and solubilize many normally water-insoluble compounds. Ferrocene and its derivatives, tetrathiafulvalene and tetramethylbenzidine, can function as redox mediators, but are insoluble in water; when they are complexed with ...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/S0167-7799(00)89002-2
更新日期：1995-11-01 00:00:00
abstract：:Starting with the Protein Data Bank (PDB) as a common ancestor, the evolution of structural databases has been driven by the rapprochement of the structural world and the practical applications. The result is an impressive number of secondary structural databases that is welcomed by structural biologists and bioinform...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0167-7799(02)02082-6
更新日期：2002-12-01 00:00:00
abstract：:It is possible to improve the sensitivity of immunoassays by several orders of magnitude by exploiting nanoenvironmental effects. This approach can detect trace amounts of compounds and will better illuminate the presence of signal substances in biological systems. Here we describe a method for ultrasensitive immunoas...
journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2017.06.001
更新日期：2017-11-01 00:00:00
