Monitoring the efficacy of bioremediation.

abstract：:Intracellular delivery of therapeutic antibodies is highly desirable but remains a challenge for biomedical research and the pharmaceutical industry. Approximately two-thirds of disease-associated targets are found inside the cell. Difficulty blocking these targets with available drugs creates a need for technology to...

journal_title：Trends in biotechnology

authors： Gordon RE,Nemeth JF,Singh S,Lingham RB,Grewal IS

更新日期：2020-08-14 00:00:00

abstract：:In Europe the commercialization of food derived from genetically modified plants has been slow because of the complex regulatory process and the concerns of consumers. Risk assessment is focused on potential adverse effects on humans and the environment, which could result from unintended effects of genetic modificati...

journal_title：Trends in biotechnology

authors： Rischer H,Oksman-Caldentey KM

更新日期：2006-03-01 00:00:00

abstract：:Dual-use research, which results in knowledge that can be used for both good and ill, has become increasingly accessible in the internet age to both scientists and the general public. Here, we outline some major milestones for dual-use policy and present three vignettes that highlight contemporary dual-use issues in b...

journal_title：Trends in biotechnology

authors： Getz LJ,Dellaire G

更新日期：2018-12-01 00:00:00

abstract：:Starting with the Protein Data Bank (PDB) as a common ancestor, the evolution of structural databases has been driven by the rapprochement of the structural world and the practical applications. The result is an impressive number of secondary structural databases that is welcomed by structural biologists and bioinform...

journal_title：Trends in biotechnology

authors： Carugo O,Pongor S

更新日期：2002-12-01 00:00:00

abstract：:Psychrophilic anaerobic treatment is an attractive option for wastewaters that are discharged at moderate to low temperature. The expanded granular sludge bed (EGSB) reactor has been shown to be a feasible system for anaerobic treatment of mainly soluble and pre-acidified wastewater at temperatures of 5--10 degrees C....

journal_title：Trends in biotechnology

authors： Lettinga G,Rebac S,Zeeman G

更新日期：2001-09-01 00:00:00

abstract：:Nanomaterials have been developed for many biomedical applications, including medical imaging, drug delivery, and antimicrobial coatings. Intriguingly, nanoparticles can display 'enzyme-like' activity and have been explored as alternatives to natural enzymes in several industrial and energy-related applications. Recen...

journal_title：Trends in biotechnology

authors： Cormode DP,Gao L,Koo H

更新日期：2018-01-01 00:00:00

abstract：:Gastrointestinal cancers rank second in overall cancer-related deaths. Carotenoids, sulfated polysaccharides, and polyunsaturated fatty acids (PUFAs) from microalgae exhibit cancer chemopreventive features at different stages of carcinogenesis. For instance, sulfated polysaccharides bear a prophylactic potential via b...

journal_title：Trends in biotechnology

authors： Amaro HM,Barros R,Guedes AC,Sousa-Pinto I,Malcata FX

更新日期：2013-02-01 00:00:00

abstract：:This review summarizes synthetic chromosome construction in plants and speculates on how they might be applied to various uses in the future, as well as the possible impact for synthetic biology. The procedures to construct foundational platforms for synthetic chromosomes in plants are described, together with some ch...

journal_title：Trends in biotechnology

authors： Birchler JA

更新日期：2015-03-01 00:00:00

abstract：:Direct delivery of purified Cas9 protein with guide RNA into plant cells, as opposed to plasmid-mediated delivery, displays high efficiency and reduced off-target effects. Following regeneration from edited cells, the ensuing plant is also likely to bypass genetically modified organism (GMO) legislation as the genome ...

journal_title：Trends in biotechnology

authors： Kanchiswamy CN,Malnoy M,Velasco R,Kim JS,Viola R

更新日期：2015-09-01 00:00:00

abstract：:Graphene is the basic building block of 0D fullerene, 1D carbon nanotubes, and 3D graphite. Graphene has a unique planar structure, as well as novel electronic properties, which have attracted great interests from scientists. This review selectively analyzes current advances in the field of graphene bioapplications. I...

journal_title：Trends in biotechnology

authors： Wang Y,Li Z,Wang J,Li J,Lin Y

更新日期：2011-05-01 00:00:00

abstract：:One of the goals of synthetic biology is to build regulatory circuits that control cell behavior, for both basic research purposes and biomedical applications. The ability to build transcriptional regulatory devices depends on the availability of programmable, sequence-specific, and effective synthetic transcription f...

journal_title：Trends in biotechnology

authors： Jusiak B,Cleto S,Perez-Piñera P,Lu TK

更新日期：2016-07-01 00:00:00

abstract：:The mapping of the human genome was completed earlier this year and efforts are underway to understand the role of gene products (i.e. proteins) in biological pathways and human disease and to exploit their functional roles to derive protein therapeutics and protein-based drugs. A key component to the next revolution ...

journal_title：Trends in biotechnology

authors： Maggio ET,Ramnarayan K

更新日期：2001-07-01 00:00:00

abstract：:Low production cost is a key factor driving the development of plants and plant tissue cultures for the synthesis of therapeutic and other foreign proteins. Because product yield and concentration exert a major influence on process economics, improving foreign protein accumulation is crucial for enhancing the commerci...

journal_title：Trends in biotechnology

authors： Doran PM

更新日期：2006-09-01 00:00:00

abstract：:Microbial exopolysaccharides such as xanthan and dextran have been commercial products for many years; the search for new gelling agents has yielded gellan. Exopolysaccharides have many other novel properties to offer, and the discovery of immune modulation and tumouristasis by beta-D-glucans, and the use of bacterial...

journal_title：Trends in biotechnology

authors： Sutherland IW

更新日期：1998-01-01 00:00:00

abstract：:There is a need for techniques that ensure antibiotic removal in anaerobic digesters for robust methane production. In this article, we discuss recent strategies for enhanced antibiotic removal from swine wastewater and offer insights on anaerobic digestion (AD) process design for improved antibiotic removal. ...

journal_title：Trends in biotechnology

authors： Zhou Q,Li X,Wu S,Zhong Y,Yang C

更新日期：2021-01-01 00:00:00

abstract：:Two of the primary responses in a cell when perturbed are modulation of the dynamics of its constituent gene expression and protein abundance to restore steady-state homeostasis. To obtain a detailed model of the restoration of this balance, it is necessary to examine the kinetics of transcription and translation, thu...

journal_title：Trends in biotechnology

authors： Hughes C,Krijgsveld J

更新日期：2012-12-01 00:00:00

abstract：:The hand-held gene gun provides a rapid and efficient method of incorporating fluorescent dyes into cells, a technique that is becoming known as diolistics. Transporting fluorescent dyes into cells has, in the past, used predominantly injection or chemical methods. The use of the gene gun, combined with the new genera...

journal_title：Trends in biotechnology

authors： O'Brien JA,Lummis SC

更新日期：2007-11-01 00:00:00

abstract：:The bioeconomy concept is proliferating globally. However, the enabling roles of biotechnology may be getting sidelined in the strategies of some countries. A goal for engineering biology is alignment with the engineering design cycle to enable more rapid commercialization. This paper considers several policy options ...

journal_title：Trends in biotechnology

authors： Kitney R,Adeogun M,Fujishima Y,Goñi-Moreno Á,Johnson R,Maxon M,Steedman S,Ward S,Winickoff D,Philp J

更新日期：2019-09-01 00:00:00

abstract：:Reconstituted viral envelopes (RVEs) are formed by solubilizing intact virus in detergent and reassembling the envelope on removal of detergent. RVEs can be formed in the presence of agents that become encapsulated and can then be utilized in vitro and in vivo for drug delivery, cell destruction, transfer of membrane ...

journal_title：Trends in biotechnology

authors： Blumenthal R,Loyter A

更新日期：1991-02-01 00:00:00

abstract：:Antibodies are able to both bind antigens and trigger the responses that eliminate them from circulation. All antibodies are glycosylated at conserved positions in their constant regions, and the presence of carbohydrate can be critical for antigen clearance functions such as complement activation. The structure of th...

journal_title：Trends in biotechnology

authors： Wright A,Morrison SL

更新日期：1997-01-01 00:00:00

abstract：:Step by step, the components of an artificial form of cellular life are being assembled by researchers. Lipid vesicles the size of small bacteria can be prepared and under certain conditions are able to grow and divide, then grow again. Polymerase enzymes encapsulated in the vesicles can synthesize RNA from externally...

journal_title：Trends in biotechnology

authors： Deamer D

更新日期：2005-07-01 00:00:00

abstract：:Peptide nucleic acids (PNAs) are novel DNA mimics in which the sugar-phosphate backbone has been replaced with a backbone based on amino acids. PNAs exhibit sequence-specific binding to DNA and RNA with higher affinities and specificities than unmodified DNA. They are resistant to nuclease and protease attack in serum...

journal_title：Trends in biotechnology

authors： Buchardt O,Egholm M,Berg RH,Nielsen PE

更新日期：1993-09-01 00:00:00

abstract：:Patterned regular sieves and filters with comparable molecular dimensions hold great promise as an alternative to conventional polymeric gels and fibrous membranes to improve biomolecule separation. Recent developments of microfabricated nanofluidic sieves and filters have demonstrated superior performance for both an...

journal_title：Trends in biotechnology

authors： Fu J,Mao P,Han J

更新日期：2008-06-01 00:00:00

abstract：:Molecular systems in which fluorescence switches between 'on' and 'off' states when driven by chemical stimuli can be designed according to a few principles. The photochemical mechanisms examined are photoinduced electron transfer, internal charge transfer and excimer formation, with emphasis on the first category. Th...

journal_title：Trends in biotechnology

authors： de Silva AP,Fox DB,Moody TS,Weir SM

更新日期：2001-01-01 00:00:00

abstract：:In this article, the current status of membrane bioreactor (MBR) technology for wastewater treatment is reviewed. Fundamental facets of the MBR process and membrane and process configurations are outlined and the advantages and disadvantages over conventional suspended growth-based biotreatment are briefly identified....

journal_title：Trends in biotechnology

authors： Judd S

更新日期：2008-02-01 00:00:00

abstract：:Platelet-derived products (PDPs) have gained popularity, mainly due to their high concentrations of bioactive molecules such as growth factors and cytokines, which play important roles in tissue healing and regeneration. PDPs are obtained through minimally invasive procedures and their therapeutic effect has been wide...

journal_title：Trends in biotechnology

authors： Soares CS,Babo PS,Reis RL,Carvalho PP,Gomes ME

更新日期：2020-08-28 00:00:00

abstract：:Cultured mammalian cells are the main workhorses for producing biologics. The performance of these cell culture processes, in terms of both productivity and product quality attributes, is significantly influenced by cellular metabolism. Glucose is the major carbon source for cellular biosynthesis and energy generation...

journal_title：Trends in biotechnology

authors： Mulukutla BC,Yongky A,Le T,Mashek DG,Hu WS

更新日期：2016-08-01 00:00:00

abstract：:Study of mutations that improve enzyme properties reveals that in many, but not all, cases closer mutations are more effective than distant ones. For enantioselectivity, substrate selectivity and new catalytic activity (catalytic promiscuity) closer mutations improved enzymes more effectively than distant ones. Howeve...

journal_title：Trends in biotechnology

authors： Morley KL,Kazlauskas RJ

更新日期：2005-05-01 00:00:00

abstract：:Genome engineering is crucial for answering fundamental questions about, and exploring practical applications of, microorganisms. Various microbial genome-engineering tools, including CRISPR/Cas-enhanced homologous recombination (HR), have been developed, with ever-improving simplicity, efficiency, and applicability. ...

journal_title：Trends in biotechnology

authors： Wang Y,Liu Y,Zheng P,Sun J,Wang M

更新日期：2021-02-01 00:00:00

abstract：:Photodynamic therapy (PDT) involves the use of a class of molecules that are activated by light, and which accumulate in, and are retained by, certain abnormal tissues. When these molecules (known as photosensitizers) are activated by light, they cause the formation of active forms of oxygen, which result in the killi...

journal_title：Trends in biotechnology

authors： Levy JG

更新日期：1995-01-01 00:00:00