Abstract:
:Peptides possess several attractive features when compared to small molecule and protein therapeutics, such as high structural compatibility with target proteins, the ability to disrupt protein-protein interfaces, and small size. Efficient design of high-affinity peptide ligands via rational methods has been a major obstacle to the development of this potential drug class. However, structural insights into the architecture of protein-peptide interfaces have recently culminated in several computational approaches for the rational design of peptides that target proteins. These methods provide a valuable alternative to experimental high-resolution structures of target protein-peptide complexes, bringing closer the dream of in silico designed peptides for therapeutic applications.
journal_name
Trends Biotechnoljournal_title
Trends in biotechnologyauthors
Vanhee P,van der Sloot AM,Verschueren E,Serrano L,Rousseau F,Schymkowitz Jdoi
10.1016/j.tibtech.2011.01.004subject
Has Abstractpub_date
2011-05-01 00:00:00pages
231-9issue
5eissn
0167-7799issn
1879-3096pii
S0167-7799(11)00014-Xjournal_volume
29pub_type
杂志文章,评审abstract::Direct delivery of purified Cas9 protein with guide RNA into plant cells, as opposed to plasmid-mediated delivery, displays high efficiency and reduced off-target effects. Following regeneration from edited cells, the ensuing plant is also likely to bypass genetically modified organism (GMO) legislation as the genome ...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2015.04.002
更新日期:2015-09-01 00:00:00
abstract::Dual-use research, which results in knowledge that can be used for both good and ill, has become increasingly accessible in the internet age to both scientists and the general public. Here, we outline some major milestones for dual-use policy and present three vignettes that highlight contemporary dual-use issues in b...
journal_title:Trends in biotechnology
pub_type: 历史文章,杂志文章
doi:10.1016/j.tibtech.2018.07.016
更新日期:2018-12-01 00:00:00
abstract::Rapid prototyping (RP) technologies are a set of manufacturing processes that can produce very complex structures directly from computer-aided design models without structure-specific tools or knowledge. These technologies might eventually enable the manufacture of human livers to create functional substitutes for tre...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2007.08.010
更新日期:2007-11-01 00:00:00
abstract::Success in synthetic biology and metabolic engineering is quickly becoming 'test' limited within the design-build-test cycle. Commonly used methods for high-throughput screening, including fluorescence-activated cell sorting (FACS) and microtiter plates, have intracellular product and throughput limitations. A growing...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2019.11.002
更新日期:2020-07-01 00:00:00
abstract::Since its invention at the beginning of the 1990s, antibody phage display has revolutionized the generation of monoclonal antibodies and their engineering. It is now possible to create antibodies binding to any chosen target antigen without the use of laboratory animals or hybridomas, in a system that completely by-pa...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/S0167-7799(97)84205-9
更新日期:1997-02-01 00:00:00
abstract::Adsorption in stable expanded beds enables proteins to be recovered directly from particulate-containing feedstocks, such as fermentation broths and preparations of disrupted cells, without the need for prior removal of the suspended solids, which would normally result in the blockage of packed beds. The adoption of t...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/0167-7799(94)90046-9
更新日期:1994-08-01 00:00:00
abstract::Benzylisoquinoline alkaloids (BIAs) are a family of ∼2500 alkaloids with both potential and realized pharmaceutical value, including most notably the opiates such as codeine and morphine. Only a few BIAs accumulate readily in plants, which limits the pharmaceutical potential of the family. Shifting BIA production to m...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2015.12.005
更新日期:2016-03-01 00:00:00
abstract::Graphene is the basic building block of 0D fullerene, 1D carbon nanotubes, and 3D graphite. Graphene has a unique planar structure, as well as novel electronic properties, which have attracted great interests from scientists. This review selectively analyzes current advances in the field of graphene bioapplications. I...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2011.01.008
更新日期:2011-05-01 00:00:00
abstract::The redundancy of natural product biosynthesis in microbes poses a practical challenge for discovering new antimicrobial compounds from bacteria. The recent application of clustered regularly interspaced short palindromic repeats (CRISPR) technology by Culp et al. to inactivate the production of abundant antibiotics g...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2019.11.003
更新日期:2020-01-01 00:00:00
abstract::One of the goals of synthetic biology is to build regulatory circuits that control cell behavior, for both basic research purposes and biomedical applications. The ability to build transcriptional regulatory devices depends on the availability of programmable, sequence-specific, and effective synthetic transcription f...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2015.12.014
更新日期:2016-07-01 00:00:00
abstract::The BRICS countries (Brazil, Russia, India, China, South Africa) account for 25% of global biotechnology patents. To understand the current and future landscape of the domain, it is important to better understand the capacity of these contributors. Here, we consider the thematic priorities, strategies, and key players...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2017.11.008
更新日期:2018-07-01 00:00:00
abstract::Peptide nucleic acids (PNAs) are novel DNA mimics in which the sugar-phosphate backbone has been replaced with a backbone based on amino acids. PNAs exhibit sequence-specific binding to DNA and RNA with higher affinities and specificities than unmodified DNA. They are resistant to nuclease and protease attack in serum...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/0167-7799(93)90097-S
更新日期:1993-09-01 00:00:00
abstract::The need for companion diagnostics, point-of-care testing (POCT) and high-throughput screening in clinical diagnostics and personalized medicine has pushed the need for more biological information from a single sample at extremely low concentrations and volumes. Optical biosensors based on semiconductor quantum dots (...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2012.04.005
更新日期:2012-07-01 00:00:00
abstract::Although there is still some skepticism in the biological community regarding the value and significance of quantitative computational modeling, important steps are continually being taken to enhance its accessibility and predictive power. We view these developments as essential components of an emerging 'respectful m...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2016.12.008
更新日期:2017-06-01 00:00:00
abstract::Carotenoids are one of the most diverse classes of natural compounds. Plant carotenoids are composed of a C40 isoprenoid skeleton with or without epoxy, hydroxy and keto groups. They have fundamental roles in human nutrition as antioxidants and vitamin A precursors and their consumption is increasingly associated with...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2007.12.003
更新日期:2008-03-01 00:00:00
abstract::Flow cytometry is a rapid method for the quantitative measurement of light scattering and fluorescent properties of cells. Although this technique has been widely applied to biomedical and environmental studies, its potential as a tool in ecotoxicological studies has not yet been fully exploited. This article describe...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0167-7799(01)01924-2
更新日期:2002-04-01 00:00:00
abstract::It is possible to improve the sensitivity of immunoassays by several orders of magnitude by exploiting nanoenvironmental effects. This approach can detect trace amounts of compounds and will better illuminate the presence of signal substances in biological systems. Here we describe a method for ultrasensitive immunoas...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2017.06.001
更新日期:2017-11-01 00:00:00
abstract::Anywhere water is in the liquid state, bacteria will exist as biofilms, which are complex communities of cells that are cemented together. Although frequently associated with disease and biofouling, biofilms are also important for engineering applications, such as bioremediation, biocatalysis and microbial fuel cells....
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2010.11.001
更新日期:2011-02-01 00:00:00
abstract::The drive for industrial sustainability has pushed biosurfactants to the top of the agenda of many companies. Biosurfactants offer the possibility of replacing chemical surfactants, produced from nonrenewable resources, with alternatives produced from cheap renewable feedstocks. Biosurfactants are also attractive beca...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2012.07.003
更新日期:2012-11-01 00:00:00
abstract::Since its inception a decade ago, sol-gel encapsulation has opened up an intriguing new way to immobilize biological materials. An array of substances, including catalytic antibodies, DNA, RNA, antigens, live bacterial, fungal, plant and animal cells and whole protozoa, have been encapsulated in silica, metal-oxide, o...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0167-7799(00)01457-8
更新日期:2000-07-01 00:00:00
abstract::Antibodies are able to both bind antigens and trigger the responses that eliminate them from circulation. All antibodies are glycosylated at conserved positions in their constant regions, and the presence of carbohydrate can be critical for antigen clearance functions such as complement activation. The structure of th...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/S0167-7799(96)10062-7
更新日期:1997-01-01 00:00:00
abstract::Nitrous oxide (N(2)O), the third most abundant greenhouse gas (GHG), is highly stable and plays a significant role in stratospheric ozone destruction. The primary anthropogenic source of N(2)O stems from use of nitrogen fertilizers in soil. The bacterial enzyme nitrous oxide reductase (N(2)OR), naturally found in some...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2012.04.002
更新日期:2012-08-01 00:00:00
abstract::The concept of ecological 'traps' is based in theory from ecology and conservation biology that has now found application to infectious diseases with a study from Paul Turner's group. This study is important because it offers a mathematical model of ecological traps, applies this model to viruses, and tests the model ...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2007.08.013
更新日期:2007-12-01 00:00:00
abstract::This article reports on the state-of-the-art technologies that sequence DNA using miniaturized devices. The article considers the miniaturization of existing technologies for sequencing DNA and the opportunities for cost reduction that 'on-chip' devices can deliver. The ability to construct nano-scale structures and p...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2007.07.001
更新日期:2007-09-01 00:00:00
abstract::Centromeres are a conundrum; although many proteins associated with centomeres are conserved from yeast to humans, the underlying DNA sequence is not. A proposed solution to this problem is that an epigenetic, largely heterochromatic, state be imposed by these proteins. Recent analysis of a human neocentromere and the...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2004.04.015
更新日期:2004-07-01 00:00:00
abstract::In vitro antibody generation technologies have now been available for two decades. Research reagents prepared via phage display are becoming available and several recent studies have demonstrated that these technologies are now sufficiently advanced to facilitate generation of a comprehensive renewable resource of ant...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/j.tibtech.2010.05.001
更新日期:2010-07-01 00:00:00
abstract::Porous microcarriers are new support materials with important advantages in both industrial cell-culture processes and the culture of cells of medical importance. Porous microcarriers are now commercially available with internal architecture and surface chemistry suitable for culture of both anchorage-dependent and an...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/0167-7799(90)90154-p
更新日期:1990-05-01 00:00:00
abstract::Luminescence has assumed an important role in analytical biochemistry and molecular biology as an extremely sensitive method for determining the concentration of specific ions and molecules. The luminescent system of the jellyfish Aequorea victoria consists of the photoprotein aequorin, which contains the molecule coe...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0167-7799(99)01379-7
更新日期:1999-12-01 00:00:00
abstract::The hand-held gene gun provides a rapid and efficient method of incorporating fluorescent dyes into cells, a technique that is becoming known as diolistics. Transporting fluorescent dyes into cells has, in the past, used predominantly injection or chemical methods. The use of the gene gun, combined with the new genera...
journal_title:Trends in biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.tibtech.2007.07.014
更新日期:2007-11-01 00:00:00
abstract::There are many aims associated with the optimization of fermentation processes. Optimization is expected to increase the yield of the final product but the process must be compliant with good manufacturing practices, the available equipment and the expected final scale of operation. Dealing with genetically modified m...
journal_title:Trends in biotechnology
pub_type: 杂志文章
doi:10.1016/s0167-7799(02)01913-3
更新日期:2002-03-01 00:00:00