Transgenic plants as vaccine production systems.

abstract：:Type 1 Diabetes (T1D) is an autoimmune disease resulting from the destruction of pancreatic insulin-producing beta cells that most frequently occurs in genetically predisposed children. Recent observations illustrating the regenerative capability of the endocrine pancreas in addition to advances in stem cell and gene ...

journal_title：Trends in biotechnology

authors： Pasquali L,Fan Y,Trucco M,Ringquist S

更新日期：2006-11-01 00:00:00

abstract：:The analysis of biomolecules using microarrays and other biosensors has a significant role in molecular biotechnology, and will become even more important in the future as a versatile tool for research and diagnostics. For many applications, the synthetic DNA mimic peptide nucleic acid (PNA) could be advantageous as a...

journal_title：Trends in biotechnology

authors： Brandt O,Hoheisel JD

更新日期：2004-12-01 00:00:00

abstract：:Proteins found in nature have traditionally been the most frequently used biocatalysts to produce numerous natural products ranging from commodity chemicals to pharmaceuticals. Protein engineering has emerged as a powerful biotechnological toolbox in the development of metabolic engineering, particularly for the biosy...

journal_title：Trends in biotechnology

authors： Li C,Zhang R,Wang J,Wilson LM,Yan Y

更新日期：2020-07-01 00:00:00

abstract：:Genetic engineering methods based on the use of transgenes have been successfully adopted to improve crops. A novel all-native DNA gene technology consists of the creation of intragenic constructs by isolating genetic elements from a crop, rearranging them in vitro, and inserting them back into the plant. The ever-inc...

journal_title：Trends in biotechnology

authors： Molesini B,Pii Y,Pandolfini T

更新日期：2012-02-01 00:00:00

abstract：:Antibodies are able to both bind antigens and trigger the responses that eliminate them from circulation. All antibodies are glycosylated at conserved positions in their constant regions, and the presence of carbohydrate can be critical for antigen clearance functions such as complement activation. The structure of th...

journal_title：Trends in biotechnology

authors： Wright A,Morrison SL

更新日期：1997-01-01 00:00:00

abstract：:Current biopharmaceutical manufacturing strongly relies on using purification platform processes, offering harmonization of practices and speed-to-market. However, the ability of such processes to respond quickly to anticipated higher quality and capacity demands is under question. Here, we describe novel approaches f...

journal_title：Trends in biotechnology

authors： Nfor BK,Verhaert PD,van der Wielen LA,Hubbuch J,Ottens M

更新日期：2009-12-01 00:00:00

abstract：:Spheroids provide a 3D environment with intensive cell-cell contacts. As a result of their excellent regenerative properties and rapid progress in their high-throughput production, spheroids are increasingly suggested as building blocks for tissue engineering. In this review, we focus on innovative biotechnological ap...

journal_title：Trends in biotechnology

authors： Laschke MW,Menger MD

更新日期：2017-02-01 00:00:00

abstract：:Recently, several groups have published data on the shear sensitivity of suspended animal cells and the protective effect of certain polymers. These findings did not, at the time, seem to have great practical application because shear sensitivity did not cause great problems for large-scale applications in sparged and...

journal_title：Trends in biotechnology

authors： van der Pol L,Tramper J

更新日期：1998-08-01 00:00:00

abstract：:In 2006, the group of Dr C.M. Lieber pioneered the field of nanowire sensors by fabricating devices for the ultra-sensitive label-free detection of biological macromolecules. Since then, nanowire sensors have demonstrated their ability to detect cancer-associated analytes in peripheral blood, tumor tissue, and the exh...

journal_title：Trends in biotechnology

authors： Doucey MA,Carrara S

更新日期：2019-01-01 00:00:00

abstract：:The use of antibodies or antibody fragments for targeting tumors (either for tumor imaging or as carriers for drugs or toxins), has encountered problems of clearance, and non-specific or inefficient binding in clinical trials. A novel approach, linking two antibody variable fragments (Fvs), with a short peptide to gen...

journal_title：Trends in biotechnology

authors： Bird RE,Walker BW

更新日期：1991-04-01 00:00:00

abstract：:Clustered regularly interspaced short palindromic repeats (CRISPR)-mediated manipulation of genomic information is becoming more versatile by combining nuclease-deficient CRISPR systems with a wide variety of effectors including base-editing deaminases, transcriptional regulators, and epigenetic modifiers. The program...

journal_title：Trends in biotechnology

authors： Mitsunobu H,Teramoto J,Nishida K,Kondo A

更新日期：2017-10-01 00:00:00

abstract：:A major goal of material science is to produce hierarchical materials that are ordered on all length scales, from the molecular (1-100 A) via the nano (10-100 nm) to the meso (1-100 microm). In these materials, the larger-scale properties can be controlled by choosing molecular characteristics. Methods developed to pr...

journal_title：Trends in biotechnology

authors： Dan N

更新日期：2000-09-01 00:00:00

abstract：:Building robust manufacturing processes from biological components is a task that is highly complex and requires sophisticated tools to describe processes, inputs, and measurements and administrate management of knowledge, data, and materials. We argue that for bioengineering to fully access biological potential, it w...

journal_title：Trends in biotechnology

authors： Sadowski MI,Grant C,Fell TS

更新日期：2016-03-01 00:00:00

abstract：:It is possible to improve the sensitivity of immunoassays by several orders of magnitude by exploiting nanoenvironmental effects. This approach can detect trace amounts of compounds and will better illuminate the presence of signal substances in biological systems. Here we describe a method for ultrasensitive immunoas...

journal_title：Trends in biotechnology

authors： Mattiasson B,Teeparuksapun K,Lebogang L,Hedström M

更新日期：2017-11-01 00:00:00

abstract：:Techniques such as positron-emission tomography, single-photon-emission computed tomography, functional magnetic-resonance imaging and magnetoencephalography permit the observation of biological processes in the brain in a noninvasive manner. They have yielded new insights into the biological interrelations of sensory...

journal_title：Trends in biotechnology

authors： Müller-Gärtner HW

更新日期：1998-03-01 00:00:00

abstract：:Hydrogels are 3D hydrophilic polymer networks that absorb and hold huge amounts of water. Although hydrogels have traditionally been synthesized using chemical and physical methods, rapid developments in enzyme technology that, like chemical-based methods, enable the formation of stable covalent bonds are fast emergin...

journal_title：Trends in biotechnology

authors： Guebitz GM,Nyanhongo GS

更新日期：2018-10-01 00:00:00

abstract：:Along with the development of metabolic engineering and synthetic biology tools, various microbes are being used to produce aromatic chemicals. In microbes, aromatics are mainly produced via a common important precursor, chorismate, in the shikimate pathway. Natural or non-natural aromatics have been produced by engin...

journal_title：Trends in biotechnology

authors： Noda S,Kondo A

更新日期：2017-08-01 00:00:00

abstract：:Antimicrobial resistance (AMR) has developed into a huge threat to global health, and reducing it is an urgent priority for public health authorities. The importance of a healthy and balanced gut microbiome has been identified as a key protective factor against AMR development, but this can be significantly affected b...

journal_title：Trends in biotechnology

authors： Kelly SA,Rodgers AM,O'Brien SC,Donnelly RF,Gilmore BF

更新日期：2020-04-01 00:00:00

abstract：:Atmospheric pressure gas plasmas are emerging as a promising treatment in cancer that can supplement the existing set of treatment modalities and, when combined with other therapies, enhance their selectivity and efficacy against resistant cancers. With further optimisation in production and administration of plasma t...

journal_title：Trends in biotechnology

authors： Dai X,Bazaka K,Richard DJ,Thompson ERW,Ostrikov KK

更新日期：2018-11-01 00:00:00

abstract：:Infrared spectroscopy and microscopy have heralded a period of rapid advances in tissue and cellular characterization during the past decade. However, vibrational spectroscopy is still an analytical tool that is neither familiar nor understood in the medical environment. For many years this field has been mainly drive...

journal_title：Trends in biotechnology

authors： Dumas P,Sockalingum GD,Sulé-Suso J

更新日期：2007-01-01 00:00:00

abstract：:Many of the demands in the production of microalgae at a technical scale cannot presently be met by state-of-the-art cultivation technologies based on suspensions. Immobilized cultivation using porous substrate bioreactors (PSBRs) is characterized by a reduction of liquid reaction volumes by several orders of magnitud...

journal_title：Trends in biotechnology

authors： Podola B,Li T,Melkonian M

更新日期：2017-02-01 00:00:00

abstract：:Carotenoids are micronutrients important for human health. The continuous improvements in clustered regularly interspaced short palindromic repeats (CRISPR)-based genome-editing techniques make rapid, DNA/transgene-free and targeted multiplex genetic modification a reality, thus promising to accelerate the breeding an...

journal_title：Trends in biotechnology

authors： Zheng X,Kuijer HNJ,Al-Babili S

更新日期：2020-12-29 00:00:00

abstract：:Soft biofunctional and biocompatible interfaces on solids designed by the deposition of ultrathin soft polymer films or supported membranes have numerous scientific and practical applications. These include the immobilization of glycolipids, membrane receptors and proteins to generate models of cell and tissue surface...

journal_title：Trends in biotechnology

authors： Sackmann E,Tanaka M

更新日期：2000-02-01 00:00:00

abstract：:Anywhere water is in the liquid state, bacteria will exist as biofilms, which are complex communities of cells that are cemented together. Although frequently associated with disease and biofouling, biofilms are also important for engineering applications, such as bioremediation, biocatalysis and microbial fuel cells....

journal_title：Trends in biotechnology

authors： Wood TK,Hong SH,Ma Q

更新日期：2011-02-01 00:00:00

abstract：:Plant-based environmental remediation, or phytoremediation, has been widely pursued in recent years as a favorable clean-up technology and is an area of intensive scientific investigation. For the vast majority of field applications, vegetative 'phyto-crops' are selected specifically for their capacity for site decont...

journal_title：Trends in biotechnology

authors： Rugh CL

更新日期：2004-10-01 00:00:00

abstract：:Microbial exopolysaccharides such as xanthan and dextran have been commercial products for many years; the search for new gelling agents has yielded gellan. Exopolysaccharides have many other novel properties to offer, and the discovery of immune modulation and tumouristasis by beta-D-glucans, and the use of bacterial...

journal_title：Trends in biotechnology

authors： Sutherland IW

更新日期：1998-01-01 00:00:00

abstract：:Rapid prototyping (RP) technologies are a set of manufacturing processes that can produce very complex structures directly from computer-aided design models without structure-specific tools or knowledge. These technologies might eventually enable the manufacture of human livers to create functional substitutes for tre...

journal_title：Trends in biotechnology

authors： Wang X,Yan Y,Zhang R

更新日期：2007-11-01 00:00:00

abstract：:Life science in the 21st century is developing rapidly through the structural analysis of biomolecules, the completion of the human genome sequence and the analysis of transcriptomes. The mouse transcriptome has been comprehensively analyzed using a gene discovery approach to collect full-length cDNA (FL-cDNA) clones....

journal_title：Trends in biotechnology

authors： Hayashizaki Y,Kanamori M

更新日期：2004-04-01 00:00:00

abstract：:The extensive use of chemical insecticides for insect pest management has resulted in insecticide resistance now being recorded in >500 species of insects and mites. Although gut-active toxins such as those derived from Bacillus thuringiensis (Bt) have been successfully used for insect pest management, a diverse range...

journal_title：Trends in biotechnology

authors： Bonning BC,Chougule NP

更新日期：2014-02-01 00:00:00

abstract：:A key aspect of the genomics revolution is the transformation of large amounts of biological information into an electronic format, leading to an information-based approach to biomedical problems. Large-scale RNA assays and gene-expression-microarray studies, in particular, represent the second wave of the genomics re...

journal_title：Trends in biotechnology

authors： Zweiger G

更新日期：1999-11-01 00:00:00