Abstract：
:GEC1 protein shares high identity with GABARAP (GABA(A) Receptor-Associated Protein), interacts with tubulin and GABA(A) receptors and is potentially involved in intracellular transport processes. Recently, using quantitative real time PCR, we have reported the gec1 mRNA expression in different rat brain areas. In the present study, we investigated the cell types expressing gec1 in rat brain. Sense and anti-sense gec1 RNA probes, corresponding to the 3'-untranslated region, were generated. In northern blotting experiments, the anti-sense probe revealed only the 1.75 kb gec1 mRNAs. On the other hand, in immunohistochemistry experiments, GEC1 polyclonal antibodies did not discriminate between GEC1 and GABARAP proteins. Therefore, we used digoxigenin-labeled RNA probes for in situ hybridization (ISH) experiments to map the gec1 expression. Using the anti-sense probe, we detected the gec1 mRNAs specifically in neurons throughout the rostrocaudal extent of the brain as well as in the spinal cord. Although a majority of neurons expressed the gec1 mRNAs, different intensities of labeling were observed depending on the areas: the strongest labeling was observed in the isocortex, hippocampus, basal telencephalon, some thalamic and most of hypothalamic nuclei, cerebellum, and numerous brainstem nuclei. Furthermore, the gec1 mRNAs were intensely expressed in neurons involved in somatomotor and neuroendocrine functions and weakly expressed in sensory and reticular structures. These results corroborate the putative role of the GEC1 protein in the trafficking of receptor GABA(A).
journal_name
Brain Resjournal_title
Brain researchauthors
Tolle F,Risold PY,Mansuy-Schlick V,Rossi E,Boyer-Guittaut M,Fraichard A,Jouvenot Mdoi
10.1016/j.brainres.2008.02.077subject
Has Abstractpub_date
2008-05-19 00:00:00pages
103-15eissn
0006-8993issn
1872-6240pii
S0006-8993(08)00564-7journal_volume
1210pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:Earlier studies in this laboratory have shown that partial serotonergic (5-HT) deafferentation from the hippocampus induced a homotypic sprouting. The 5-HT-denervated hippocampus was further found to be a favorable environment for the growth of grafted 5-HT, but not for norepinephrinergic neurons. In the current study...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)90286-k
更新日期：1990-01-22 00:00:00
abstract：:Morphological alterations occurring in Schwann cells of unmyelinated fibers (unmyelinated Schwann cells) were investigated in the sciatic nerve of the twicher mouse, a murine model of human globoid cell leukodystrophy. After postnatal day 10, the number of Schwann cell-axon units gradually increased and the number of ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)91719-8
更新日期：1989-11-27 00:00:00
abstract：:Focal axonal swellings are characteristic of a wide range of neuropathies. Three neuron-specific monoclonal antibodies have been used to identify focal axonal swellings in the normal developing rat cerebellar cortex. Between 7 and 15 days postnatal, swellings are a common feature of the granular layer and white matter...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)91018-8
更新日期：1986-01-22 00:00:00
abstract：:Microtubule organization in type I hair cells has been investigated during the synaptogenesis of vestibular receptors in mammals. The different steps in the maturation of the synapse between the hair cell and the nerve chalice were: a slight symmetrical membrane densification; the appearance of synaptic bodies alongsi...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(86)90161-6
更新日期：1986-02-01 00:00:00
abstract：:Using the teased fiber recording method, we have compared pathophysiological properties of afferent axons injured in the infraorbital nerve (ION) vs the sciatic nerve in rats. Both myelinated and unmyelinated axons ending in ION neuromas produced much less ongoing discharge than those ending in sciatic nerve neuromas....
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)90753-v
更新日期：1992-05-01 00:00:00
abstract：:Regeneration of severed hypothalamic peptidergic neurosecretory axons into the ventral pia-arachnoid was observed in rats at the light microscopic and fine-structural levels. A temporal increase occurred in the number of neurophysin-positive axons regenerating into the leptomeninges for distances up to 3.3 mm by 40 da...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)91557-0
更新日期：1988-05-31 00:00:00
abstract：:Hybridization histochemical and immunohistochemical methods were used to characterize the distribution of corticotropin-releasing factor (CRF) messenger RNA (mRNA) and peptide, respectively, in the central auditory system of the rat. Cell bodies expressing CRF mRNA and/or immunoreactivity (IR) were detected at each le...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)90571-c
更新日期：1991-04-26 00:00:00
abstract：:Astrogliogenesis in the human fetal cerebellum was examined in 46 cerebella obtained from hysterotomy specimens ranging between 9 and 20 weeks of ovulation age. By correlating the results obtained by rapid Golgi and Golgi-Cox methods, the indirect immunofluorescence technique for glial fibrillary acidic protein, and e...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(80)90280-2
更新日期：1980-05-26 00:00:00
abstract：:To verify whether microtubules are involved in the mechanism of axoplasmic transport in vivo, [3H]leucine was injected into ventral horns of rats, and 3 h later Ca2+ or other drugs injected into sciatic nerves. The injection of 50-200 mM Ca2+, raising intra-axoplasmic Ca2+ levels, blocked transport above the intraneur...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)90514-7
更新日期：1986-03-26 00:00:00
abstract：:The glutamate terminals in rat rostral neostriatum were removed on one side by ipsilateral frontal cortical ablation. Intraperitoneal administration of L-methioninesulfoximine (MSO), an inhibitor of glutamine synthetase, induced different changes in amino acid concentration in the rostral neostriatum on the intact and...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(85)90261-6
更新日期：1985-07-08 00:00:00
abstract：:The effects of pituitary adenylate cyclase activating polypeptides (PACAPs: PACAP27, PACAP38) on glutamate-induced neurotoxicity were examined using cultured retinal neurons obtained from 3- to 5-day old Wistar rats. Cell viability was evaluated by double staining with fluorescein diacetate and propidium iodide. Effec...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(99)01690-x
更新日期：1999-08-21 00:00:00
abstract：:The neurotrophic effect of bFGF on dopamine (DA) neurons in vitro depends upon its mitogenic activity on glial cells. We examined whether glial secretion of the dopaminergic growth factor GDNF is responsible for the trophic activity of bFGF on mesencephalic DA neurons. When added with lower concentrations of bFGF, GDN...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(96)00311-3
更新日期：1996-06-10 00:00:00
abstract：:In the present study, the light responses of ganglion cells to chromatic stimulus were recorded from isolated retina of neonatal chick. It was found that for some non-color-opponent ganglion cells, the spatiotemporal patterns of the cells' light responses were related to the chromatic information that they received. W...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2005.07.077
更新日期：2005-10-12 00:00:00
abstract：:We have examined the effects of isotonic hypovolemia on the expression of the neuronal nitric oxide synthase (nNOS) gene in the paraventricular (PVN) and supraoptic nuclei (SON) of the rat, using in situ hybridization histochemistry with a 35S-labelled oligodeoxynucleotide probe complementary to nNOS mRNA. Intraperito...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)01531-x
更新日期：1998-04-20 00:00:00
abstract：:Blockade of gamma-aminobutyric acid (GABA) function in the posterior hypothalamus of rats elicits a pattern of physiological and behavioral arousal consisting of increases in heart rate, respiration and blood pressure as well as intense locomotor stimulation and a selective enhancement of avoidance responding. The pre...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)91173-e
更新日期：1990-03-26 00:00:00
abstract：:We compared the clinical and pharmacodynamic effects of N-terminal methionine brain-derived neurotrophic factor (met-BDNF) and endogenous met-free BDNF in wobbler mouse motor neuron disease (MND). Met- or met-free BDNF at 5 or 20 mg/kg was subcutaneously injected daily, six times/week for 4 weeks. At 20 mg/kg, grip st...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)02881-0
更新日期：2002-07-19 00:00:00
abstract：:The distinction between neural mechanisms of explicit and implicit expressions of memory has been well studied at the retrieval stage, but less at encoding. In addition, dissociations obtained in many studies are complicated by methodological difficulties in obtaining process-pure measures of different types of memory...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2014.08.024
更新日期：2014-10-17 00:00:00
abstract：:To test the hypothesis that stimulation of glial peroxidase activity by estrogens may play a role in the pathogenesis of the previously reported degenerative changes in the hypothalamic arcuate nucleus that occur in female rats treated with a long-acting estrogen preparation, the cellular localization and number of pe...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)90273-e
更新日期：1990-01-22 00:00:00
abstract：:Pineal glands of male Syrian hamsters stimulated in vivo with isoproterenol (ISO) for 4 h before the onset of darkness showed a 4-h advance in the timing of the nighttime increases in both N-acetyltransferase activity and melatonin levels. When ISO (1 mg/kg) was administered every 2 h to animals kept in light during t...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)90633-6
更新日期：1988-12-20 00:00:00
abstract：:Arg-vasopressin (AVP) containing neurons are one of the output paths from the suprachiasmatic nucleus (SCN), the center of the biological clock. AVP mRNA transcription is controlled by a negative feedback loop of clock genes. Circadian rhythm of melatonin release from the pineal gland is regulated by the SCN via the p...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2004.04.052
更新日期：2004-07-09 00:00:00
abstract：:Tumor necrosis factor-alpha (TNFalpha) affects several CNS functions such as regulation of sleep, body temperature, and feeding during pathology. There is also evidence for TNFalpha involvement in physiological sleep regulation, e.g., TNFalpha induces sleep and brain levels of TNFalpha increase during prolonged wakefu...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2005.06.044
更新日期：2005-08-16 00:00:00
abstract：:We have examined, at the light microscope level, putative direct synaptic interconnections, via motor axon collaterals, between type-identified triceps surae alpha-motoneurons labeled by intracellular injection of HRP. The results indicate that monosynaptic recurrent contacts can occur between synergist motoneurons (i...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)90937-5
更新日期：1984-08-06 00:00:00
abstract：:The density of protein gene product 9.5- and tyrosine hydroxylase-immunoreactive nerve fibers innervating circumvallate papillae of the tongue was substantially increased in transgenic mice that overexpressed nerve growth factor (NGF) when compared with age-matched controls. The fiber density was age-dependent. Only t...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)01336-9
更新日期：1996-01-29 00:00:00
abstract：:Structures of the medial temporal lobes are recognized to play a central role in memory processing and to be the primary sites of deterioration in Alzheimer disease (AD). Mild cognitive impairment (MCI) represents potentially an intermediate state between normal aging and AD. Proton magnetic resonance spectroscopy (MR...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2003.11.074
更新日期：2004-04-02 00:00:00
abstract：:While much has been elucidated about the hypothalamic controls of energy balance, the epidemic of obesity continues to escalate. Recent work has suggested that extra-hypothalamic central nervous system structures may play a previously un-appreciated role in the control of ingestive behavior and body weight regulation....
journal_title：Brain research
pub_type： 杂志文章,评审
doi：10.1016/j.brainres.2010.06.009
更新日期：2010-09-02 00:00:00
abstract：:'Feedback' signals from mammalian extrastriate visual cortices are reported to exert primarily an excitatory influence on the classical receptive field (CRF) of neurons in the primary visual cortex (V1). However, given the much larger CRFs of neurons in extrastriate visual cortices it is not yet understood how feedbac...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.02.069
更新日期：2010-04-30 00:00:00
abstract：:To determine the involvement of the hippocampal regions in a operant-type delayed alternation task of short delay or long delay, microinjections of muscimol into the hippocampus were used for temporal inactivation during the behavioral test in each rat. Dorsal hippocampal inactivation impaired the correct ratio of lon...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(01)02084-4
更新日期：2001-03-23 00:00:00
abstract：:Glucose inhibits ∼60% of lateral hypothalamic (LH) orexin neurons. Fasting increases the activation of LH orexin glucose-inhibited (GI) neurons in low glucose. Increases in spontaneous glutamate excitatory postsynaptic currents (sEPSCs) onto putative VTA DA neurons in low glucose are orexin dependent (Sheng et al., 20...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2018.05.025
更新日期：2020-03-15 00:00:00
abstract：:We have investigated the receptor subtype(s) mediating the noradrenergic inhibition of cerebellar Purkinje cell spontaneous firing rate using local application of specific agonists and antagonists, in situ, via pressure microejection. Extracellular action potentials were recorded from Purkinje neurons in anesthetized ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)90552-5
更新日期：1988-10-18 00:00:00
abstract：:Our previous study reported that cathepsin L may contribute to the death of dopaminergic neurons in rodent model of Parkinson's disease (PD). In this study we detected the changes in the expression of lysosomal cathepsin L in cellular models of PD. In human neuroblastoma SH-SY5Y cells, treatment with 6-hydroxydopamine...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2011.02.092
更新日期：2011-04-28 00:00:00
