Single-cell genomic sequencing using Multiple Displacement Amplification.

abstract：:Microbial growth can be characterized by a limited set of macroscopic parameters such as growth rate, biomass yield and substrate affinity. Different culturing protocols for laboratory evolution have been developed to select mutant strains that have one specific macroscopic growth parameter improved. Some of those mut...

journal_title：Current opinion in microbiology

authors： Bachmann H,Bruggeman FJ,Molenaar D,Branco Dos Santos F,Teusink B

更新日期：2016-06-01 00:00:00

abstract：:The post-translational attachment of ubiquitin or ubiquitin-like modifiers (ULMs) to proteins regulates many cellular processes including the generation of innate and adaptive immune responses to pathogens. Vice versa, pathogens counteract immune defense by inhibiting or redirecting the ubiquitination machinery of the...

journal_title：Current opinion in microbiology

authors： Viswanathan K,Früh K,DeFilippis V

更新日期：2010-08-01 00:00:00

abstract：:Since the past decades, oligosaccharides are considered for their potential biological activities. To exploit them, it was essential to obtain pure molecules in large amounts. Several strategies were developed to produce specific sugar sequences with specific substitution patterns from land plants and algae polysaccha...

journal_title：Current opinion in microbiology

authors： Courtois J

更新日期：2009-06-01 00:00:00

abstract：:Some of the most successful drugs in the antibiotic pharmacopeia are those that inhibit bacterial cell wall biosynthesis. However, the worldwide spread of bacterial antibiotic resistance has eroded the clinical efficacy of these drugs and the antibiotic pipeline continues to be lean as drug discovery programs struggle...

journal_title：Current opinion in microbiology

authors： Gale RT,Brown ED

更新日期：2015-10-01 00:00:00

abstract：:An understanding of the molecular bases of pathogenicity in Blastomyces dermatitidis and related systemic dimorphic fungi has been limited until recent years. Yeast cells of B. dermatitidis display an adhesion promoting protein termed WI-1. Recent studies entailing homologous gene targeting and mutation of WI-1 have p...

journal_title：Current opinion in microbiology

authors： Klein BS

更新日期：2000-08-01 00:00:00

abstract：:The inexorable rise in cases of tuberculosis worldwide, fuelled by the HIV epidemic, highlights the need for new drugs and particularly those that can shorten the duration of treatment. Clinical trials of existing broad-spectrum agents such as the fluoroquinolone moxifloxacin are proceeding, on the basis of efficacy i...

journal_title：Current opinion in microbiology

authors： Duncan K,Barry CE 3rd

更新日期：2004-10-01 00:00:00

abstract：:Recent studies based on metagenomics and other molecular techniques have permitted a detailed knowledge of the microbial diversity and metabolic activities of microorganisms in hypersaline environments. The current accepted model of community structure in hypersaline environments is that the square archaeon Haloquadra...

journal_title：Current opinion in microbiology

authors： Ventosa A,de la Haba RR,Sánchez-Porro C,Papke RT

更新日期：2015-06-01 00:00:00

abstract：:One defining feature of apicomplexan parasites is their special ability to actively invade host cells. Although rapid, invasion is a complicated process that requires coordinated activities of host cell attachment, protein secretion, and motility by the parasite. Central to this process is the establishment of a struc...

journal_title：Current opinion in microbiology

authors： Shen B,Sibley LD

更新日期：2012-08-01 00:00:00

abstract：:Asexual sporulation (conidiation) in the ascomycetous filamentous fungi involves the formation of conidia, formed on specialized structures called conidiophores. Conidiation in filamentous fungi involves many common themes including spatial and temporal regulation of gene expression, specialized cellular differentiati...

journal_title：Current opinion in microbiology

authors： Park HS,Yu JH

更新日期：2012-12-01 00:00:00

abstract：:The eukaryotic unicellular pathogen Plasmodium falciparum tightly regulates gene expression, both during development and in adaptation to dynamic host environments. This regulation is evident in the mutually exclusive expression of members of clonally variant virulence multigene families. While epigenetic regulators h...

journal_title：Current opinion in microbiology

authors： Vembar SS,Scherf A,Siegel TN

更新日期：2014-08-01 00:00:00

abstract：:RNA sequencing has emerged as the premier approach to study bacterial transcriptomes. While the earliest published studies analyzed the data qualitatively, the data are readily digitized and lend themselves to quantitative analysis. High-resolution RNA sequence (RNA-seq) data allows transcriptional features (promoters...

journal_title：Current opinion in microbiology

authors： Creecy JP,Conway T

更新日期：2015-02-01 00:00:00

abstract：:Trypanosomes and Leishmania contain an abundance of stage-regulated cysteine proteinases encoded by several gene families. Analysis of parasites rendered defective in cysteine proteinase function, either through genetic manipulation or through the use of specific inhibitors, has revealed roles for the enzymes in paras...

journal_title：Current opinion in microbiology

authors： Mottram JC,Brooks DR,Coombs GH

更新日期：1998-08-01 00:00:00

abstract：:Despite the widespread availability of genome sequences and the systematic assignment of individual open reading frames, many of the activities of metabolic enzymes are still veiled. It is difficult to assign biochemical functions to enzymes and uncover the in vivo regulation of their enzymatic activities, but this is...

journal_title：Current opinion in microbiology

authors： Saito N,Ohashi Y,Soga T,Tomita M

更新日期：2010-06-01 00:00:00

abstract：:Mosquitoes are the major arthropod vectors of human diseases such as malaria and viral encephalitis. However, each mosquito species does not transmit every pathogen, owing to reasons that include specific evolutionary histories, mosquito immune system structure, and ecology. Even a competent vector species for a patho...

journal_title：Current opinion in microbiology

authors： Mitri C,Vernick KD

更新日期：2012-06-01 00:00:00

abstract：:Arginine is an integral part of host defense when invading pathogens are encountered. The arginine metabolite nitric oxide (NO) confers antimicrobial properties, whereas the metabolite ornithine is utilized for polyamine synthesis. Polyamines are crucial to tissue repair and anti-inflammatory responses. iNOS/arginase ...

journal_title：Current opinion in microbiology

authors： Gogoi M,Datey A,Wilson KT,Chakravortty D

更新日期：2016-02-01 00:00:00

abstract：:Bacterial shape has always been hypothesized to play an important role in the biology of a species and in the ability of certain bacteria to influence human health. The recent discovery of peptidoglycan hydrolases that modulate shape has now allowed this hypothesis to be addressed directly. Genetic, biochemical, and p...

journal_title：Current opinion in microbiology

authors： Frirdich E,Gaynor EC

更新日期：2013-12-01 00:00:00

abstract：:Candida albicans is a regular member of the intestinal microbiota in the majority of the human population. This underscores C. albicans' adaptation to life in the intestine without inducing competitive interactions with other microbes, or immune responses detrimental to its survival. However, specific conditions such ...

journal_title：Current opinion in microbiology

authors： Kumamoto CA,Gresnigt MS,Hube B

更新日期：2020-08-01 00:00:00

abstract：:Viruses attach to specific receptors, but identified receptors are often absent from the epithelial surfaces where infections begin. Viruses can bind to alternative receptor molecules present on epithelial surfaces and they can enter hosts in ways that bypass mucosal barriers to infection. To understand how viruses cr...

journal_title：Current opinion in microbiology

authors： Bergelson JM

更新日期：2003-08-01 00:00:00

abstract：:Complete and long-lasting protective immunity against malaria can be achieved through vaccination with invasive live attenuated Plasmodium sporozoites, the motile stage inoculated in the host skin during a mosquito bite. Protective immunity relies primarily on effector CD8+ T cells targeting the parasite in the liver....

journal_title：Current opinion in microbiology

authors： Silvie O,Amino R,Hafalla JC

更新日期：2017-12-01 00:00:00

abstract：:There is accumulating evidence that motile bacteria are chemotactically attracted to environmental pollutants that they can degrade. Chemotaxis, the ability of motile bacteria to detect and respond to specific chemicals in the environment, can increase an organism's chances of locating useful sources of carbon, nitrog...

journal_title：Current opinion in microbiology

authors： Parales RE,Harwood CS

更新日期：2002-06-01 00:00:00

abstract：:Environmental pathogenic fungi present a paradox in that they are virulent in animals without requiring animal hosts for replication or survival, a phenomenon we call 'ready-made' virulence. In the human pathogenic fungus Cryptococcus neoformans, the capacity for virulence in animals may originate from environmental s...

journal_title：Current opinion in microbiology

authors： Casadevall A,Steenbergen JN,Nosanchuk JD

更新日期：2003-08-01 00:00:00

abstract：:One promise of synthetic biology is to provide solutions for biomedical and industrial problems by rational design of added functionality in living systems. Microbes are at the forefront of this biological engineering endeavor due to their general ease of handling and their relevance in many potential applications fro...

journal_title：Current opinion in microbiology

authors： Bittihn P,Din MO,Tsimring LS,Hasty J

更新日期：2018-10-01 00:00:00

abstract：:Extracytoplasmic function σ factors (ECFs) represent a fundamental and widely distributed principle of bacterial signal transduction that connects the perception of a stimulus (input) with the induction of an appropriate set of genes (output). In recent years, comparative genomics analyses have not only allowed a syst...

journal_title：Current opinion in microbiology

authors： Mascher T

更新日期：2013-04-01 00:00:00

abstract：:Many filamentous nitrogen-fixing cyanobacteria protect nitrogenase from oxygen in differentiated cells called heterocysts. Heterocyst development is controlled by the availability of nitrogen compounds in the environment and by intrinsic factors that regulate the frequency and pattern of heterocysts along vegetative c...

journal_title：Current opinion in microbiology

authors： Golden JW,Yoon HS

更新日期：2003-12-01 00:00:00

abstract：:Inflammasomes - molecular platforms for caspase-1 activation - have emerged as common hubs for a number of pathways that detect and respond to bacterial pathogens. Caspase-1 activation results in the secretion of bioactive IL-1β and IL-18 and pyroptosis, and thus launches a systemic immune and inflammatory response. I...

journal_title：Current opinion in microbiology

authors： Eldridge MJ,Shenoy AR

更新日期：2015-02-01 00:00:00

abstract：:The historical paradigm of the deep ocean as a biological 'desert' has shifted to one of a 'rainforest' owing to the isolation of many novel microbes and their associated bioactive compounds. Recently, there has been an explosion of information about novel bioactive compounds that have been isolated from marine microb...

journal_title：Current opinion in microbiology

authors： Zhang L,An R,Wang J,Sun N,Zhang S,Hu J,Kuai J

更新日期：2005-06-01 00:00:00

abstract：:Atomic force microscopy imaging and force spectroscopy have recently opened a range of novel applications in microbiology. During the past two years, rapid advances have been made using atomic force microscopy to visualize the surface structure of two-dimensional bacterial protein crystals, biofilms and individual cel...

journal_title：Current opinion in microbiology

authors： Dufrêne YF

更新日期：2003-06-01 00:00:00

abstract：:One current challenge in metabolic systems biology is to map out the regulation networks that control metabolism. From progress in this area, we conclude that non-transcriptional mechanisms (e.g. metabolite-protein interactions and protein phosphorylation) are highly relevant in actually controlling metabolic function...

journal_title：Current opinion in microbiology

authors： Heinemann M,Sauer U

更新日期：2010-06-01 00:00:00

abstract：:All organisms must secure essential trace nutrients, including iron, zinc, manganese and copper for survival and proliferation. However, these very nutrients are also highly toxic if present at elevated levels. Mammalian immunity has harnessed both the essentiality and toxicity of micronutrients to defend against micr...

journal_title：Current opinion in microbiology

authors： Ballou ER,Wilson D

更新日期：2016-08-01 00:00:00

abstract：:Macrophages protect their host by engulfing foreign bodies within phagosomes that rapidly develop into microbicidal organelles. Numerous pathogens, such as species of Toxoplasma, Leishmania, Mycobacterium, Salmonella and Legionella, thrive in human macrophages, sometimes with disastrous effects. Defining the survival ...

journal_title：Current opinion in microbiology

authors： Amer AO,Swanson MS

更新日期：2002-02-01 00:00:00