Abstract:
:Atomic force microscopy imaging and force spectroscopy have recently opened a range of novel applications in microbiology. During the past two years, rapid advances have been made using atomic force microscopy to visualize the surface structure of two-dimensional bacterial protein crystals, biofilms and individual cells in physiological conditions. There has also been remarkable progress in using force spectroscopy to measure biomolecular interactions and physical properties of microbial surfaces. Specific highlights include the imaging and manipulation of membrane proteins at the subnanometer level, the observation of the surface of living cells at high resolution, the mapping of local properties such as surface charges, the measurement of elastic properties of cell-surface constituents and the probing of cellular interactions using functionalized probes.
journal_name
Curr Opin Microbioljournal_title
Current opinion in microbiologyauthors
Dufrêne YFdoi
10.1016/s1369-5274(03)00058-4subject
Has Abstractpub_date
2003-06-01 00:00:00pages
317-23issue
3eissn
1369-5274issn
1879-0364pii
S1369527403000584journal_volume
6pub_type
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