Abstract:
:Antisense technology has been widely used to regulate gene expression. A tetracycline (tet)-regulated antisense-RNA-expressing system has been developed and used to downregulate chromosomally derived genes expressed in Staphylococcus aureus. This downregulation subsequently provides an evaluation of the virulence factor and drug targets. The regulated antisense RNA library allows for genome-wide analyses of the functions of staphylococcal gene products for growth in culture and survival during infection. Moreover, this antisense RNA technology may provide a key tool to identify mechanisms of novel antibacterial compound action.
journal_name
Curr Opin Microbioljournal_title
Current opinion in microbiologyauthors
Yin D,Ji Ydoi
10.1016/s1369-5274(02)00315-6subject
Has Abstractpub_date
2002-06-01 00:00:00pages
330-3issue
3eissn
1369-5274issn
1879-0364pii
S1369527402003156journal_volume
5pub_type
杂志文章,评审abstract::Motile bacteria seek optimal living habitats by following gradients of attractant and repellent chemicals in their environment. The signaling machinery for these chemotactic behaviors, although assembled from just a few protein components, has extraordinary information-processing capabilities. Escherichia coli, the be...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
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abstract::High throughput, deep sequencing assays are powerful tools for gaining insights into virus-host interactions. Sequencing assays can discover novel viruses and describe the genomes of novel and known viruses. Genomic information can predict viral proteins that can be characterized, describe important genes in the host ...
journal_title:Current opinion in microbiology
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abstract::Pyrrolysine is an amino acid encoded by the amber codon in genes required for methylamine utilization by members of the Methanosarcinaceae. Pyrrolysine and selenocysteine share the distinction of being the only two non-canonical amino acids that have entered natural genetic codes. Recent experiments have shown that en...
journal_title:Current opinion in microbiology
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journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2014.02.003
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abstract::Pneumococcal conjugate vaccine is not only effective against invasive and non-invasive disease in all ages but also has a significant impact on antibiotic-resistant infections since a number of resistant serotypes are targeted by the vaccine. Surveillance studies in the post-licensure years have shown an increase in n...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2007.08.008
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abstract::Wild Escherichia coli are superbly adapted to survive in the intestines of their mammalian hosts and in the environment. E. coli K12 derivative (MG1655) encodes 4288 potential genes that provide the background genetic framework of this species. Particular E. coli clonal types encode additional chromosomal and extrachr...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/s1369-5274(00)00170-3
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abstract::An understanding of the molecular bases of pathogenicity in Blastomyces dermatitidis and related systemic dimorphic fungi has been limited until recent years. Yeast cells of B. dermatitidis display an adhesion promoting protein termed WI-1. Recent studies entailing homologous gene targeting and mutation of WI-1 have p...
journal_title:Current opinion in microbiology
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abstract::Major advances have been made in the past five years in the identification of cellular targets of toxins produced by anaerobic bacteria. These targets include the vesicular membrane docking and fusion apparatus, the actin cytoskeleton, the signal transduction machinery and the cell membrane. The recent discovery that ...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/s1369-5274(98)80144-6
更新日期:1998-02-01 00:00:00
abstract::The three dimensional ultrastructure of cells and tissues comes to light with tomography. There is an inherent tension between representing molecular detail at the highest possible resolution, on one hand, and visualizing spatial relations between large organelles or even neighboring cells in large volumes, on the oth...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
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abstract::While natural microbial communities are composed of a mix of microbes with often unknown functions, the construction of synthetic microbial communities allows for the generation of defined systems with reduced complexity. Used in a top-down approach, synthetic communities serve as model systems to ask questions about ...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2014.02.002
更新日期:2014-04-01 00:00:00
abstract::The enzymological and genetic aspects of microbial metabolism of hydrocarbons have been extensively revealed. Such molecular information is useful for understanding the bioremediation of oil spill environments and production of hydrocarbon-specific fine chemicals. ...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/s1369-5274(03)00053-5
更新日期:2003-06-01 00:00:00
abstract::Single microbial cells can now be sequenced using DNA amplified by the Multiple Displacement Amplification (MDA) reaction. The few femtograms of DNA in a bacterium are amplified into micrograms of high molecular weight DNA suitable for DNA library construction and Sanger sequencing. The MDA-generated DNA also performs...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2007.08.005
更新日期:2007-10-01 00:00:00
abstract::Staphylococcus aureus is a leading cause of hospital-acquired and often persistent infections. A key feature of pathogenic S. aureus is the expression of an array of extracellular matrix-binding proteins. In particular, the fibronectin-binding proteins FnBP-A and FnBP-B afford the pathogen the ability to connect to ce...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2005.12.002
更新日期:2006-02-01 00:00:00
abstract::Upon infection, both DNA and RNA viruses can be sensed by pattern recognition receptors (PRRs) in the cytoplasm or the nucleus to activate antiviral innate immunity. Sensing of viral products leads to the activation of a signaling cascade that ultimately results in transcriptional activation of type I and III interfer...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2016.05.015
更新日期:2016-08-01 00:00:00
abstract::Small RNAs can act to regulate both the synthesis of proteins, by affecting mRNA transcription, translation and stability, and the activity of specific proteins by binding to them. As a result of recent genome-wide screens, around 50 small RNAs have now been identified in Escherichia coli. These include many that requ...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/s1369-5274(03)00027-4
更新日期:2003-04-01 00:00:00
abstract::Some RNA virus genomes bear 5'-triphosphates, which can be recognized in the cytoplasm of infected cells by host proteins that mediate anti-viral immunity. Both the innate sensor RIG-I and the interferon-induced IFIT proteins bind to 5'-triphosphate viral RNAs. RIG-I signals for induction of interferons during RNA vir...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2013.04.009
更新日期:2013-08-01 00:00:00
abstract::Intracellular protozoans co-evolved with their mammalian host cells a range of strategies to cope with the composite and dynamic cell surface features they encounter during migration and infection. Therefore, these single-celled eukaryotic parasites represent a fascinating source of living probes for precisely capturi...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2019.06.007
更新日期:2019-12-01 00:00:00
abstract::Bacteria utilize a diverse set of nucleotide second messengers to regulate cellular responses by binding macromolecular receptors (RNAs and proteins). Recent studies on cyclic di-GMP (c-di-GMP) have shown that this signaling molecule binds multiple receptors to regulate different steps in the same biological process. ...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2016.08.010
更新日期:2016-12-01 00:00:00
abstract::Schizosaccharomyces pombe is a useful model system for understanding many aspects of eukaryotic cell growth. Studies of S. pombe have identified novel genes that function in the regulation of iron homeostasis. In response to high levels of iron, Fep1 represses the expression of several genes involved in the acquisitio...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2013.07.007
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abstract::Natural hypersaline waters are widely distributed around the globe, as both continental surface waters and sea floor lakes, the latter being maintained by the large density difference between the hypersaline and overlying marine water. Owing to the extreme salt concentrations, close to or at saturation (approximately ...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2007.05.017
更新日期:2007-08-01 00:00:00
abstract::Recently, a number of endophytic fungi have been discovered that produce volatile organic compounds (VOCs) whilst growing on agricultural waste substrates, whose chemistry is best defined as hydrocarbon and hydrocarbon-like. These compounds have potential use as both 'green chemicals' and fuels. This report discusses ...
journal_title:Current opinion in microbiology
pub_type: 杂志文章
doi:10.1016/j.mib.2014.06.003
更新日期:2014-06-01 00:00:00
abstract::The review highlights the dual role that the conserved master regulator FoxO has in aging by coordinating both stem cell proliferation and antimicrobial peptides, effector molecules of the innate immune system. As observed in non-senescent Hydra, continuously high activity of the transcription factor FoxO contributes ...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2017.06.009
更新日期:2017-08-01 00:00:00
abstract::Huge numbers of enzymes have evolved in nature to function in aqueous environments at moderate temperatures and neutral pH. This gives us, in principle, the unique opportunity to construct multistep reaction systems of considerable catalytic complexity in vitro. However, this opportunity is rarely exploited beyond res...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2007.05.009
更新日期:2007-06-01 00:00:00
abstract::One promise of synthetic biology is to provide solutions for biomedical and industrial problems by rational design of added functionality in living systems. Microbes are at the forefront of this biological engineering endeavor due to their general ease of handling and their relevance in many potential applications fro...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2018.02.009
更新日期:2018-10-01 00:00:00
abstract::Sequestered from the action of complement, antibody and lytic peptides, phagosomal pathogens pose a unique problem for the innate immune system both in terms of detection and disposal. An immunologically induced 47-kDa (p47) GTPase family recruited to nascent phagosomes (PGs) has provided new insights into how vertebr...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2004.12.012
更新日期:2005-02-01 00:00:00
abstract::Fluency in a common language allows individuals to convey information and carry out complex activities that otherwise would be difficult or even impossible without the benefit of shared communication. Cyclic (di)nucleotides have recently been recognized as such an accessible language understood by both microbe and the...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2015.12.005
更新日期:2016-04-01 00:00:00
abstract::The historical paradigm of the deep ocean as a biological 'desert' has shifted to one of a 'rainforest' owing to the isolation of many novel microbes and their associated bioactive compounds. Recently, there has been an explosion of information about novel bioactive compounds that have been isolated from marine microb...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2005.04.008
更新日期:2005-06-01 00:00:00
abstract::Coxiella burnetii is a widespread zoonotic bacterial pathogen that causes human Q fever. In vivo, Coxiella displays a tropism for mononuclear phagocytes where it participates in biogenesis of a lysosome-like replication compartment to conduct its obligate intracellular lifestyle. Coxiella actively regulates multiple e...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2008.11.005
更新日期:2009-02-01 00:00:00
abstract::The obligatory intracellular bacterial pathogens Anaplasma and Ehrlichia infect leukocytes by hijacking host-cell components and processes. The type IV secretion system is up-regulated during infection. Among type IV secretion candidate substrates, an ankyrin repeat protein of Anaplasma phagocytophilum, AnkA, is deliv...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2009.12.008
更新日期:2010-02-01 00:00:00
abstract::Microbial communities have considerable impacts on animal health. However, only in recent years have the host factors impacting microbiome composition been explored. An increasing wealth of microbiome data in combination with decades of research on behavior, physiology, and development have resulted in the European ho...
journal_title:Current opinion in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.mib.2019.08.001
更新日期:2019-08-01 00:00:00