Genomic analysis using conditional phenotypes generated by antisense RNA.

Abstract:

:Antisense technology has been widely used to regulate gene expression. A tetracycline (tet)-regulated antisense-RNA-expressing system has been developed and used to downregulate chromosomally derived genes expressed in Staphylococcus aureus. This downregulation subsequently provides an evaluation of the virulence factor and drug targets. The regulated antisense RNA library allows for genome-wide analyses of the functions of staphylococcal gene products for growth in culture and survival during infection. Moreover, this antisense RNA technology may provide a key tool to identify mechanisms of novel antibacterial compound action.

journal_name

Curr Opin Microbiol

authors

Yin D,Ji Y

doi

10.1016/s1369-5274(02)00315-6

subject

Has Abstract

pub_date

2002-06-01 00:00:00

pages

330-3

issue

3

eissn

1369-5274

issn

1879-0364

pii

S1369527402003156

journal_volume

5

pub_type

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