Repair of potentially lethal damage by introduction of T4 DNA ligase in eucaryotic cells.


:The bacterial enzyme PvuII, which generates blunt-ended DNA double-strand breaks, and T4 DNA ligase, which seals adjacent DNA fragments in coupling to ATP cleavage, were introduced in mouse C3H10T1/2 fibroblasts using osmolytic shock of pinocytic vesicles. Cells were then assayed for their clonogenic ability. In agreement with previous studies by others, we find that the PvuII restriction endonuclease simulates ionizing radiation effects by causing a dose-dependent loss of reproductive capacity. Here we show that the concomitant treatment with DNA ligase considerably increases cell survival. Survival curves were shown to be dependent on the ligase enzyme dose and on ATP concentration in the hypertonic medium. We conclude that T4 DNA ligase is able to repair some of the potentially lethal damage produced by restriction endonucleases in eucaryotic cells.


Int J Radiat Biol


Durante M,Grossi GF,Napolitano M,Gialanella G




Has Abstract


1991-04-01 00:00:00














  • Effects of in vitro alpha-particle irradiation on osteogenic bone marrow cultures.

    abstract::Murine bone marrow contains osteogenic precursor cells that undergo differentiation during in vitro cultivation. In vitro these cells are potential target cells for alpha-irradiation-induced bone tumour formation. Under defined tissue culture conditions these differentiating cells were directly exposed to alpha-partic...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Schoeters GE,Vander Plaetse F,van den Heuvel RL

    更新日期:1994-08-01 00:00:00

  • A comparison of the chemical repair rates of free radical precursors of DNA damage and cell killing in Chinese hamster V79 cells.

    abstract::One of the important temporal stages of radiation action in cellular systems is the chemical phase, where oxygen fixation reactions compete with chemical repair reactions involving reducing agents such as GSH. Using the gas explosion technique it is possible to follow the kinetics of these fast (greater than 1 ms) rea...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Prise KM,Davies S,Michael BD

    更新日期:1992-06-01 00:00:00

  • Modification of tumour response by calcium antagonists in the SCVII/St tumour implanted at two different sites.

    abstract::Dose responses for the effects of four calcium antagonists on SCCVII/St back and leg tumour sensitivity to 20 Gy X-rays, and on tumour perfusion indicated by relative uptake of 86rubidium, were determined. The effects of these agents on 86rubidium uptake in some normal tissues were also investigated. Flunarizine sensi...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Wood PJ,Hirst DG

    更新日期:1989-09-01 00:00:00

  • Evaluation of the cytogenotoxic damage in immature and mature rats exposed to 900 MHz radiofrequency electromagnetic fields.

    abstract:PURPOSE:One of the most important issues regarding radiofrequency electromagnetic fields (RF-EMF) is their effect on genetic material. Therefore, we investigated the cytogenotoxic effects of 900 MHz radiofrequency electromagnetic fields (RF-EMF) and the effect of a recovery period after exposure to RF-EMF on bone marro...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Atlı Şekeroğlu Z,Akar A,Şekeroğlu V

    更新日期:2013-11-01 00:00:00

  • Effects of 36.6 GHz and static magnetic field on degree of endoreduplication in Drosophila melanogaster polytene chromosomes.

    abstract::Purpose To study the effect of microwave (MW) irradiation and consistent action of microwaves and static magnetic field (MF) on the giant chromosomes endoreduplication in Drosophila melanogaster Meig. Materials and methods Experiments were carried out on inbred wild type Canton-S strain. Exposure to microwaves (freque...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Dyka LD,Shakina LA,Strashnyuk VY,Shckorbatov YG

    更新日期:2016-01-01 00:00:00

  • X-ray-induced DNA double-strand breaks as lethal lesions in diploid human fibroblasts compared to Chinese hamster ovary cells.

    abstract::Calibration of the non-denaturing (pH 9.6) filter elution technique by 125I decays has been used to examine the relationship between X-ray-induced DNA double-strand breaks (dsb) and cellular survival of CHO cells and human fibroblasts. DNA dsb induction varies markedly between the two cell lines, reflecting the differ...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Baumstark-Khan C

    更新日期:1993-03-01 00:00:00

  • Modelling of radiation damage by 125I on a nucleosome.

    abstract::Studies of early physical interactions of ionizing radiation in biological medium have evolved from water cylinders or spheres to structured volumes representing nucleosomal DNA, based on spatial co-ordinates for each individual atom. Regarding the physico-chemical and chemical stages, the models of DNA have evolved f...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Terrissol M

    更新日期:1994-11-01 00:00:00

  • Minisatellite and Hprt mutations in V79 cells irradiated with helium ions and gamma rays.

    abstract:PURPOSE:To evaluate and compare cytotoxic and mutational effects of graded doses of gamma-rays and 4He++ ions at different LET values (nominally 80 and 123 keV/microm) in V79 cells. MATERIALS AND METHODS:4He++ ion beams at 80 and 123 keV/microm were supplied by the 7 MV Van de Graaff CN accelerator of the INFN-LNL in ...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Cherubinit R,Canova S,Favaretto S,Bruna V,Battivelli P,Celotti L

    更新日期:2002-09-01 00:00:00

  • Topoisomerase activities and levels in irradiated Chinese hamster AA8 cells and in its radiosensitive mutant EM9.

    abstract:PURPOSE:To investigate possible variations in topoisomerase (topo) I and II activities and levels after X-ray treatment in the radiation repair proficient AA8 Chinese hamster cell line for comparison with the radiation sensitive mutant EM9. MATERIALS AND METHODS:AA8 and EM9 cells were irradiated with 5 Gy of X-rays an...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Pastor N,Piñero J,Ortiz T,Mateos JC,De Miguel M,Cortés F

    更新日期:1999-08-01 00:00:00

  • Gold nanoparticles as dose-enhancement agent for kilovoltage X-ray therapy of melanoma.

    abstract:PURPOSE:Melanoma is mainly treated by surgery and rarely with radiation because of the high radioresistance of this tumor. Nevertheless, radiotherapy is the preferred treatment modality for unresectable lesions and avoiding cosmetic disfigurement caused by surgical excision. This study investigated the therapeutic adva...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Kim SR,Kim EH

    更新日期:2017-05-01 00:00:00

  • Pain-relieving effects of pulsed magnetic fields in a rat model of carrageenan-induced hindpaw inflammation.

    abstract:PURPOSE:Many strategies have been investigated to exclude the several side-effects of pharmacological or invasive treatments. Non-invasive pulsed magnetic field (PMF) treatment with no toxicity or side-effects can be an alternative to pharmacologic treatments. The purpose of this study was, therefore, to investigate th...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Mert T,Ocal I,Cinar E,Yalcin MS,Gunay I

    更新日期:2014-01-01 00:00:00

  • Repair of DNA strand breaks at hyperthermic temperatures in Chinese hamster ovary cells.

    abstract::The repair of DNA double-strand breaks was measured by pH 7.2 filter elution in cells incubated at 25-45 degrees C either before or after X-irradiation. Exposure to 45 degrees C for 15 minutes immediately prior to X-irradiation significantly increased both the half-time for DNA double-strand break closure and the numb...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Warters RL,Axtell J

    更新日期:1992-01-01 00:00:00

  • Detection and sequencing of ionizing radiation-induced DNA rearrangements using the inverse polymerase chain reaction.

    abstract:PURPOSE:To develop a procedure, using the inverse polymerase chain reaction, to detect and sequence ionizing radiation-induced DNA rearrangements without prior phenotypic selection of mutant cells. METHOD:Normal human fibroblast cells (IMR-90) were given 30Gy of gamma-irradiation and then incubated at 37 degrees C for...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Forrester HB,Radford IR

    更新日期:1998-07-01 00:00:00

  • Increased γ-H2AX by exposure to a 60-Hz magnetic fields combined with ionizing radiation, but not hydrogen peroxide, in non-tumorigenic human cell lines.

    abstract:PURPOSE:Genotoxic effects have been considered the gold standard to determine if an environmental factor is a carcinogen, but the currently available data for extremely low frequency time-varying magnetic fields (ELF-MF) remain controversial. As an environmental stimulus, the effect of ELF-MF on cellular DNA may be sub...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Yoon HE,Lee JS,Myung SH,Lee YS

    更新日期:2014-04-01 00:00:00

  • Retrospective analysis of double-strand break rejoining data collected using warm-lysis PFGE protocols.

    abstract::Sample preparation procedures for the pulsed-field gel electrophoresis (PFGE) assay usually involve a lysis step at temperatures as high as 50 degrees C. During this warm-lysis procedure, multiply damaged sites containing heat-labile sites (HLS) can be converted into double-strand breaks (DSB). Once formed, these DSB ...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Ratnayake RK,Semenenko VA,Stewart RD

    更新日期:2005-06-01 00:00:00

  • Increased radiation tolerance of mouse tongue epithelium after local conditioning.

    abstract::The effect of local stimulation on mitotic activity and radiation tolerance was studied in mouse tongue mucosa. Silver nitrate solution (0.5-20%) was used for local conditioning. The most effective protocol comprised three daily treatments (days 0-2), yielding a delayed increase in 24 h mitotic counts by about 30% on ...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Dörr W,Kummermehr J

    更新日期:1992-03-01 00:00:00

  • Nuclear structure and the microdistribution of radiation damage in DNA.

    abstract::Evidence for the roles of proteins and metal ions in the microheterogeneity of DNA damage is reviewed. Decondensation of chromatin in hypotonic buffers markedly sensitizes the DNA to radiation, while treatment of nuclei with hypertonic buffers strips the DNA of histones and other nuclear proteins and enhances the radi...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章,评审


    authors: Oleinick NL,Balasubramaniam U,Xue L,Chiu S

    更新日期:1994-11-01 00:00:00

  • Liposome-based delivery of a boron-containing cholesteryl ester for high-LET particle-induced damage of prostate cancer cells: a boron neutron capture therapy study.

    abstract:PURPOSE:The efficacy of a boron-containing cholesteryl ester compound (BCH) as a boron neutron capture therapy (BNCT) agent for the targeted irradiation of PC-3 human prostate cancer cells was examined. MATERIALS AND METHODS:Liposome-based delivery of BCH was quantified with inductively coupled plasma-mass spectrometr...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Gifford I,Vreeland W,Grdanovska S,Burgett E,Kalinich J,Vergara V,Wang CK,Maimon E,Poster D,Al-Sheikhly M

    更新日期:2014-06-01 00:00:00

  • The New Zealand white rabbit animal model of acute radiation syndrome: hematopoietic and coagulation-based parameters by radiation dose following supportive care.

    abstract:PURPOSE:Animal models that accurately reflect human responses to radiation injury are needed for advanced mechanistic investigation and development of effective therapeutics. The rabbit is an established animal model accepted by the FDA for studies of cardiovascular disease, lipid metabolism, the development of anticoa...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Paredes A,Lindeblad M,Patil R,Neal MD,Hong Y,Smith B,Nanda JP,Mousafeiris V,Moulder J,Bosland MC,Lyubimov A,Bartholomew A

    更新日期:2020-10-26 00:00:00

  • Substructure in the radiation survival response at low dose: asynchronous and partially synchronized V79-WNRE cells.

    abstract::The radiation survival response of asynchronously-dividing populations of the cell line V79-WNRE indicates substructure in the low dose region that can be better characterized by separately fitting the data within the low and high dose regions to the linear-quadratic (LQ) equation. Flow cytometry and cell sorting tech...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Skwarchuk MW,Wouters BG,Skarsgard LD

    更新日期:1993-11-01 00:00:00

  • TPD52 and NFKB1 gene expression levels correlate with G2 chromosomal radiosensitivity in lymphocytes of women with and at risk of hereditary breast cancer.

    abstract:PURPOSE:To evaluate a transcriptomic approach to identify healthy women at increased risk of breast cancer due to G2-radiosensitivity and look at transcripts that are differentially expressed between individuals. MATERIALS AND METHODS:We perform the first study to assess the association of G2 radiosensitivity with bas...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Sims AH,Finnon P,Miller CJ,Bouffler SD,Howell A,Scott D,Clarke RB

    更新日期:2007-06-01 00:00:00

  • Alterations in water and electrolyte absorption in the rat colon following neutron irradiation: influence of neutron component and irradiation dose.

    abstract:PURPOSE:To study the absorptive function of rat colon following whole-body exposure to neutron irradiation, either to the same total dose with varying proportion of neutrons or to the same neutron proportion with an increasing irradiation dose. MATERIALS AND METHODS:Different proportions of neutron irradiation were pr...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Dublineau I,Ksas B,Joubert C,Aigueperse J,Gourmelon P,Griffiths NM

    更新日期:2002-12-01 00:00:00

  • Chromosomal aberration types in cells at the second division after irradiation in G1 or G2.

    abstract::Cultures of JU-56 cells were irradiated in either G1 or G2 and examined either in their first post-irradiation metaphase (in diploids) or in their second post-irradiation metaphase (in colcemid-induced tetraploids). The timing of fixation, together with tritiated thymidine pulse labelling, allowed selection for scorin...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Moore RC,Bender MA

    更新日期:1993-06-01 00:00:00

  • Comet assay studies of radiation-induced DNA damage and repair in various tumour cell lines.

    abstract::We used the 'comet assay' to compare the amount of radiation-induced DNA damage in three tumour cell lines (MeWo, PECA 4451 and PECA 4197) and the extent of DNA repair in two of these lines (MeWo and PECA 4197). Tumour cells were irradiated with X-rays (0.1-10 Gy), embedded in agarose on slides, lysed with sodium dode...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Müller WU,Bauch T,Streffer C,Niedereichholz F,Böcker W

    更新日期:1994-03-01 00:00:00

  • Infrared spectroscopic demonstration of magnetic orientation in SH-SY5Y neuronal-like cells induced by static or 50 Hz magnetic fields.

    abstract::Purpose: To study the response of neuronal-like cells to an applied static or low-frequency magnetic field. Materials and methods: Fourier Transform Infrared (FTIR) Spectroscopy was used to investigate the overall behavior of SH-SY5Y neuronal-like cells exposed to a static or 50 Hz magnetic fields at intensities up to...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Calabrò E,Magazù S

    更新日期:2019-06-01 00:00:00

  • Absence of delayed chromosomal instability in a normal human fibroblast cell line after 125I iododeoxyuridine.

    abstract:PURPOSE:To seek the delayed appearance of chromosomal abnormalities in human fibroblasts exposed to the Auger electron emitter 125I. MATERIALS AND METHODS:Normal untransformed human fibroblasts, HF19, were exposed to a concentration of [I125]IUdR, which allowed the survival of 37% of clonogens. Chromosomal analysis us...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Griffin CS,Neshasateh-Riz A,Mairs RJ,Wright EG,Wheldon TE

    更新日期:2000-07-01 00:00:00

  • Role of a high throughput biodosimetry test in treatment prioritization after a nuclear incident.

    abstract:PURPOSE:We introduce and evaluate a high throughput biodosimetry test system (REDI-Dx) suitable for testing of thousands of potential radiation victims following a mass scale nuclear event caused by detonation of a nuclear device or a nuclear accident, as part of an overall strategy for effective medical management of ...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Jacobs AR,Guyon T,Headley V,Nair M,Ricketts W,Gray G,Wong JYC,Chao N,Terbrueggen R

    更新日期:2020-01-01 00:00:00

  • Clinical and epidemiological observations on individual radiation sensitivity and susceptibility.

    abstract::Purpose: To summarize existing knowledge and to understand individual response to radiation exposure, the MELODI Association together with CONCERT European Joint Programme has organized a workshop in March 2018 on radiation sensitivity and susceptibility.Methods: The workshop reviewed the current evidence on this matt...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章,评审


    authors: Seibold P,Auvinen A,Averbeck D,Bourguignon M,Hartikainen JM,Hoeschen C,Laurent O,Noël G,Sabatier L,Salomaa S,Blettner M

    更新日期:2020-03-01 00:00:00

  • Chronic exposure to bryostatin-1 increases the radiosensitivity of U937 leukaemia cells ectopically expressing Bcl-2 through a non-apoptotic mechanism.

    abstract:PURPOSE:Ionizing radiation (IR) produced a dose-dependent increase in apoptosis in U937/pCEP4 cells which was attenuated by the stable over expression of Bcl-2 (U937/Bcl-2). A dose of 2 Gy IR was selected for further analyses to determine if subsequent exposure to 10nM bryostatin- would overcome the resistance to IR-in...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Cartee L,Davis C,Lin PS,Grant S

    更新日期:2000-10-01 00:00:00

  • The long-term effects of γ-radiation on the growth of Allium cepa plants.

    abstract:PURPOSE:The purpose of this study was to assess the long-term effects of gamma-radiation, including low-dose radiation, on growth parameters of onion (Allium cepa) seedling roots 6-10 days after irradiation. MATERIALS AND METHODS:Onion seedlings were exposed to a 137Cs gamma source at doses ranging from 0.1 to 10 gray...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Bolsunovsky A,Trofimova E,Dementyev D,Petrichenkov M

    更新日期:2020-11-16 00:00:00