Repair of potentially lethal damage by introduction of T4 DNA ligase in eucaryotic cells.


:The bacterial enzyme PvuII, which generates blunt-ended DNA double-strand breaks, and T4 DNA ligase, which seals adjacent DNA fragments in coupling to ATP cleavage, were introduced in mouse C3H10T1/2 fibroblasts using osmolytic shock of pinocytic vesicles. Cells were then assayed for their clonogenic ability. In agreement with previous studies by others, we find that the PvuII restriction endonuclease simulates ionizing radiation effects by causing a dose-dependent loss of reproductive capacity. Here we show that the concomitant treatment with DNA ligase considerably increases cell survival. Survival curves were shown to be dependent on the ligase enzyme dose and on ATP concentration in the hypertonic medium. We conclude that T4 DNA ligase is able to repair some of the potentially lethal damage produced by restriction endonucleases in eucaryotic cells.


Int J Radiat Biol


Durante M,Grossi GF,Napolitano M,Gialanella G




Has Abstract


1991-04-01 00:00:00














  • Late effects of radiation on the human immune system: an overview of immune response among the atomic-bomb survivors.

    abstract::The studies of the late effects of atomic-bomb (A-bomb) radiation on the immune system were started about 20 years after the bombings in 1945. The most remarkable late effects of radiation are the functional and quantitative abnormalities of T and B cells in survivors exposed to high doses (> or = 1.0 Gy). Abnormaliti...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章,评审


    authors: Akiyama M

    更新日期:1995-11-01 00:00:00

  • Expression of transforming growth factor-beta 1 in mouse skin during the acute phase of radiation damage.

    abstract::Transforming growth factor-beta (TGF beta 1) plays a central role in wound healing, so its perturbation by radiation may contribute to the acute and late effects seen in irradiated skin. TGF beta 1 mRNA expression was measured by PCR, in the skin of the CD1 and CBA mouse, exposed to Sr-90 beta from an 11-mm diameter s...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Randall K,Coggle JE

    更新日期:1995-09-01 00:00:00

  • Effect of local exposure to inhomogeneous static magnetic field on stomatological pain sensation - a double-blind, randomized, placebo-controlled study.

    abstract:PURPOSE:Static magnetic field (SMF) exposure was shown experimentally to beneficially affect the healing process. The aim of this randomized, controlled trial was to evaluate the pain-inhibitory effect of local exposure to SMF on temporomandibular disorders, on alveolitis and on aphta in the oral mucosa. MATERIALS AND...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章,随机对照试验


    authors: László JF,Farkas P,Reiczigel J,Vágó P

    更新日期:2012-05-01 00:00:00

  • The different doses of radiation therapy-induced damage to the ovarian environment in rats.

    abstract:PURPOSE:The sequelae of premature loss of ovarian function can undoubtedly have undesirable effects for a woman although radiotherapy is one of the most relevant treatment modalities for various types of malignancies. The aim of this study was to determine the effect of different doses of radiation on ovarian folliculo...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Onder GO,Balcioglu E,Baran M,Ceyhan A,Cengiz O,Suna PA,Yıldız OG,Yay A

    更新日期:2021-01-06 00:00:00

  • Alpha particle-induced bystander effect is mediated by ROS via a p53-dependent SCO2 pathway in hepatoma cells.

    abstract:PURPOSE:The radiation-induced bystander effect (RIBE) has important implications for the efficiency of radiotherapy but the underlying role of cellular metabolism is widely unknown. The roles of synthesis of cytochrome c oxidase 2 (SCO2), a key effector for respiratory chain, and related signaling factors in α-particle...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Li J,He M,Shen B,Yuan D,Shao C

    更新日期:2013-12-01 00:00:00

  • WAM to SeeSaw model for cancer therapy - overcoming LQM difficulties.

    abstract:PURPOSE:The assessment of biological effects caused by radiation exposure has been currently carried out with the linear-quadratic (LQ) model as an extension of the linear non-threshold (LNT) model. In this study, we suggest a new mathematical model named as SeaSaw (SS) model, which describes proliferation and cell dea...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Bando M,Tsunoyama Y,Suzuki K,Toki H

    更新日期:2020-12-15 00:00:00

  • Clinical interest in determinations of cellular radiation sensitivity.

    abstract::Determinations of cell sensitivity in terms of survival fraction after doses employed in clinical radiation therapy, say 1-3 Gy, are of increasing interest to clinicians as they provide direct experimental data which can be employed without reference to models of cell inactivation. SF2 values are expected ultimately t...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章,评审


    authors: Suit HD,Baumann M,Skates S,Convery K

    更新日期:1989-11-01 00:00:00

  • Efficacy of delayed administration of sargramostim up to 120 hours post exposure in a nonhuman primate total body radiation model.

    abstract:BACKGROUND:High dose ionizing radiation exposure is associated with myelo-depression leading to pancytopenia and the expected clinical manifestations of acute radiation syndrome (ARS). Herein, we evaluated the efficacy of sargramostim (Leukine®, yeast-derived rhu GM-CSF), with regimens delivered at 48, 72, 96, or 120 h...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Zhong Y,Pouliot M,Downey AM,Mockbee C,Roychowdhury D,Wierzbicki W,Authier S

    更新日期:2020-09-22 00:00:00

  • No evidence of telomere length attrition in newborns from high level natural background radiation areas in Kerala coast, south west India.

    abstract:PURPOSE:The tandemly repeated hexamers (TTAGGG)n present at the telomeric ends protect the human genome from a variety of environmental exposures including ionizing radiation. In order to find out the effect of chronic low dose radiation exposure, we have determined telomere length among newborns from high level natura...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Das B,Saini D,Seshadri M

    更新日期:2012-09-01 00:00:00

  • Nuclear structure and the microdistribution of radiation damage in DNA.

    abstract::Evidence for the roles of proteins and metal ions in the microheterogeneity of DNA damage is reviewed. Decondensation of chromatin in hypotonic buffers markedly sensitizes the DNA to radiation, while treatment of nuclei with hypertonic buffers strips the DNA of histones and other nuclear proteins and enhances the radi...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章,评审


    authors: Oleinick NL,Balasubramaniam U,Xue L,Chiu S

    更新日期:1994-11-01 00:00:00

  • The damage to phospholipids caused by free radical attack on glycerol and sphingosine backbone.

    abstract::The effect of gamma-radiation on aqueous solutions of saturated phospholipids, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol (DPPG), 1-palmitoyl-2-lyso-sn-glycero-3-phosphocholine (lysoPC), and bovine brain sphingomyelin (SM) has been investigated. It is shown that th...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Edimecheva IP,Kisel MA,Shadyro OI,Vlasov AP,Yurkova IL

    更新日期:1997-05-01 00:00:00

  • Substructure in the radiation survival response at low dose: asynchronous and partially synchronized V79-WNRE cells.

    abstract::The radiation survival response of asynchronously-dividing populations of the cell line V79-WNRE indicates substructure in the low dose region that can be better characterized by separately fitting the data within the low and high dose regions to the linear-quadratic (LQ) equation. Flow cytometry and cell sorting tech...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Skwarchuk MW,Wouters BG,Skarsgard LD

    更新日期:1993-11-01 00:00:00

  • Comparison of DNA damage and repair following radiation challenge in buccal cells and lymphocytes using single-cell gel electrophoresis.

    abstract:PURPOSE:To develop a reproducible single-cell gel electrophoresis assay for DNA damage and repair in buccal mucosa and sublingual exfoliated cells. MATERIALS AND METHODS:Buccal mucosa and sublingual cells and lymphocytes from six individuals (three males, three females, aged 34-45 years) were challenged with increasin...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Dhillon VS,Thomas P,Fenech M

    更新日期:2004-07-01 00:00:00

  • Agarose overlay selectively improves macrocolony formation and radiosensitivity assessment in primary fibroblasts.

    abstract:PURPOSE:Primary fibroblasts are not suitable for in vitro macrocolony assay due to their inability to form distinct colonies. Here we present a modification of agarose overlay that yielded extensive improvement in their colony formation and assessment of radiosensitivity. MATERIALS AND METHODS:Macrocolony formation wa...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Chandna S,Dagur RS,Mathur A,Natarajan AT,Harms-Ringdahl M,Haghdoost S

    更新日期:2014-05-01 00:00:00

  • Adoptive transfer of cytotoxic T-cells for treatment of residual disease after irradiation.

    abstract:PURPOSE:To evaluate whether immunotherapy based on adoptively transferred cytotoxic T-cells (CTL) can improve the antitumour efficacy of irradiation. MATERIAL AND METHODS:The experiments were performed using the human squamous cell carcinoma line UT-SCC-15, which expresses human leukocyte antigen (HLA)-A2. The UT-SCC-...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Krause M,Schmitz M,Noessner E,Skrablin PS,Wehner R,Rieber EP,Baumann M

    更新日期:2007-11-01 00:00:00

  • Detection and sequencing of ionizing radiation-induced DNA rearrangements using the inverse polymerase chain reaction.

    abstract:PURPOSE:To develop a procedure, using the inverse polymerase chain reaction, to detect and sequence ionizing radiation-induced DNA rearrangements without prior phenotypic selection of mutant cells. METHOD:Normal human fibroblast cells (IMR-90) were given 30Gy of gamma-irradiation and then incubated at 37 degrees C for...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Forrester HB,Radford IR

    更新日期:1998-07-01 00:00:00

  • Radiation retards muscle differentiation but does not affect osteoblastic differentiation induced by bone morphogenetic protein-2 in C2C12 myoblasts.

    abstract:PURPOSE:Effects of radiation on the differentiation of C2C12 myoblasts were studied using cell cultures in which the cells differentiated into either muscle cells or osteoblasts. MATERIALS AND METHODS:A mouse skeletal myoblast cell line, C2C12, was cultured and irradiated at confluence. Muscle differentiation was esti...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Ikeda S,Hachisu R,Yamaguchi A,Gao YH,Okano T

    更新日期:2000-03-01 00:00:00

  • Cytotoxicity of cigarette smoke condensate is not due to DNA double strand breaks: Comparative studies using radiosensitive mutant and wild-type CHO cells.

    abstract:PURPOSE:To determine whether cigarette smoke condensate (CSC) without metabolic activation induces direct DNA double strand breaks (DSB) in the G1 phase of various radiosensitive mutants of CHO cells and whether these breaks display collateral hypersensitivity to CSC with respect to cell killing. MATERIALS & METHODS:W...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Kato T,Nagasawa H,Warner C,Okayasu R,Bedford JS

    更新日期:2007-09-01 00:00:00

  • Spin transfer from protein to DNA in X-irradiated 'dry' and hydrated chromatin: an electron spin resonance investigation of spectral components between 77 K and room temperature.

    abstract:PURPOSE:To investigate the potential total and relative free-radical transfer from histones to DNA in X-irradiated chromatin, by analysing the relative contributions of individual radicals from protein and from DNA. MATERIALS AND METHODS:Chromatin was isolated from calf thymus, freeze-dried and either used as such or ...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Weiland B,Hüttermann J

    更新日期:2000-08-01 00:00:00

  • Chromosomal radiosensitivity in BRCA1 and BRCA2 mutation carriers.

    abstract:PURPOSE:The chromosomal radiosensitivity of a selected group of familial breast cancer patients carrying a mutation in BRCA1 (n=11) or BRCA2 (n=9) and a group of healthy mutation carriers (n=12) was investigated and compared to a reference group of breast cancer patients without a BRCA1/2 mutation (n=78) and a group of...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Baeyens A,Thierens H,Claes K,Poppe B,de Ridder L,Vral A

    更新日期:2004-10-01 00:00:00

  • Bronchoalveolar lavage and interstitial cells have different roles in radiation-induced lung injury.

    abstract:PURPOSE:To determine the contribution of intra-alveolar cells as opposed to cells fixed in the interstitium in the development of radiation-induced lung injury. MATERIALS AND METHODS:C3H/HeN mice were irradiated to the thorax with various doses of radiation. The cellular composition and cytokine production were assess...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Hong JH,Jung SM,Tsao TC,Wu CJ,Lee CY,Chen FH,Hsu CH,McBride WH,Chiang CS

    更新日期:2003-03-01 00:00:00

  • 1-Methylxanthine enhances the radiosensitivity of tumor cells.

    abstract:PURPOSE:To determine the efficacy of a caffeine derivative 1-methylxanthine (1-MTX) in increasing radiosensitivity of cancer cells and elucidate the underlying mechanisms in vitro. MATERIALS AND METHODS:RKO human colorectal cancer cells carrying wild type protein 53 kDa (p53) were incubated with 3 mM 1-MTX for 30 min,...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Youn H,Hee Kook Y,Oh ET,Jeong SY,Kim C,Kyung Choi E,Uk Lim B,Park HJ

    更新日期:2009-02-01 00:00:00

  • Synergestic effect of aqueous purslane (Portulaca oleracea L.) extract and fish oil on radiation-induced damage in rats.

    abstract:PURPOSE:To evaluate the impact of oral administration of purslane (Portulaca oleracea) extract or fish oil and their co-treatments in the modulation of radiation-induced damage. MATERIAL AND METHODS:Purslane (P) (400 mg/kg body weight) or fish oil (Fo) (60 mg/kg body weight) was administrated to male albino rats via g...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Abd El-Azime AS,Hussein EM,Ashry OM

    更新日期:2014-12-01 00:00:00

  • The effects of gamma rays on the regeneration of hair follicles are carried over to later hair cycles.

    abstract:PURPOSE:The purpose of this study was to determine whether the effects of gamma rays on the regeneration of hair follicles are carried over to later hair cycles. MATERIALS AND METHODS:The whole bodies of C57BL/10JHir mice in the 1st telogen phase were irradiated with (60)Co γ-rays. Mice were examined for the effects o...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Sugaya K,Ishihara Y,Inoue S,Hirobe T

    更新日期:2015-01-01 00:00:00

  • Role of protein kinase-C in thymocyte apoptosis induced by irradiation.

    abstract::The role of protein kinase C in radiation-induced death of thymocytes was studied. For this purpose murine thymocytes were irradiated and incubated for 6 h at 37 degrees C and afterwards the fraction of fragmented DNA was measured. Results indicate that radiation-induced DNA fragmentation can be prevented by adding th...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Ojeda F,Guarda MI,Maldonado C,Folch H,Diehl H

    更新日期:1992-05-01 00:00:00

  • DNA lesion complexity and induction of apoptosis by ionizing radiation.

    abstract:PURPOSE:To determine whether murine lymphoid cell lines can discriminate between high- and low-LET (linear energy transfer) radiation-induced DNA lesions. MATERIALS AND METHODS:Sensitivity to killing by DNA-incorporated 3H and 125I decays, accumulated during storage in the gas phase of a liquid nitrogen tank, was dete...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Radford IR

    更新日期:2002-06-01 00:00:00

  • Solar radiation induced skin damage: review of protective and preventive options.

    abstract:PURPOSE:Solar energy has a number of short- and long-term detrimental effects on skin that can result in several skin disorders. The aim of this review is to summarise current knowledge on endogenous systems within the skin for protection from solar radiation and present research findings to date, on the exogenous opti...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章,评审


    authors: Svobodová A,Vostálová J

    更新日期:2010-12-01 00:00:00

  • Time course for the hazard of radiation-induced pneumonitis death in mice.

    abstract::The form of the hazard function for radiation-induced pneumonitis death in mice was investigated. 'Hazard' refers to the instantaneous failure rate at a specified time, conditional upon non-failure to that time. Thus, the hazard function describes the time profile for the risk of pneumonitis death among still-survivin...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Tucker SL,Travis EL

    更新日期:1992-11-01 00:00:00

  • Radioprotective influence of Mentha piperita (Linn) against gamma irradiation in mice: Antioxidant and radical scavenging activity.

    abstract:PURPOSE:To evaluate the radiomodulatory influence of a leaf extract of Mentha piperita (Linn) on hepatic antioxidant status and lipid peroxidation in Swiss albino mice. MATERIALS AND METHODS:Animals were given either double distilled water or leaf extract of M. piperita orally (1 g/kg bwt/day) once a day for three con...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章,收录出版


    authors: Samarth RM,Panwar M,Kumar M,Kumar A

    更新日期:2006-05-01 00:00:00

  • The relevance of tumour and surrounding normal tissue vascular density in clinical hyperthermia of locally advanced breast carcinoma.

    abstract::It follows from the present work that the vascularization of the normal tissue present in the treatment volume limits the temperatures achieved during heat treatment of invasive ductal breast carcinoma. The temperatures can often be increased by giving fractionated heat treatment because heat treatment may reduce the ...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Lyng H,Monge OR,Bøhler PJ,Rofstad EK

    更新日期:1991-07-01 00:00:00