Abstract:
PURPOSE:To investigate the possible role of cell cycle arrest in the radiosensitization of mouse spermatogonial stem cells due to small conditioning X-ray exposures. MATERIALS AND METHODS:A 24 h fractionation interval between conditioning (1 Gy) and challenging (8 or 9 Gy) exposures was used. Two approaches were followed: the first in the Swiss random-bred wild-type mouse of the radiation-induced cell cycle arrest-evading agents 3-aminobenzamide (3-AB) and caffeine; and, second, using the C57BL/6 mouse of different p53 status. As biological parameter stem cell survival was analysed by the repopulation index (RI) method and chromosomal translocations were recorded using spermatocyte analysis at appropriate posttreatment periods. RESULTS:In the Swiss wild-type mouse, the application of 3-AB or caffeine significantly suppressed the sensitization of stem cells towards killing or translocation induction. In the C57BL/6 mouse, somewhat more variability in response was observed but no significant differences in sensitization between the p53 +/+, +/- or -/- mouse were recorded, suggesting no involvement of p53 in this process. CONCLUSIONS:The results indicate that p53-independent cell cycle regulation plays an important role in the radiosensitization of mouse spermatogonial stem cells.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Van Buul PP,Van Duyn-Goedhart A,Beumer T,Bootsma ALdoi
10.1080/09553000010009035subject
Has Abstractpub_date
2001-03-01 00:00:00pages
357-63issue
3eissn
0955-3002issn
1362-3095journal_volume
77pub_type
杂志文章abstract:PURPOSE:Chironomus ramosus is one of the recently reported radiotolerant insects. Salivary gland cells of fourth instar larvae respond to ionizing radiations with increases in the levels of antioxidant enzymes and chaperone proteins. Here we made an attempt to study the state of nuclear DNA after exposure of larvae to ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2015.1062572
更新日期:2015-01-01 00:00:00
abstract::Terminally differentiated cells usually do not divide and are, thus, reproductively dead. To elucidate the significance of radiation-enhanced differentiation to reproductive cell death, murine erythroid progenitor cells were gamma-irradiated in plasma clot cultures and the development of haemoglobinized clones was stu...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096145869
更新日期:1996-03-01 00:00:00
abstract:PURPOSE:This study evaluates early changes in human mesenchymal stem cells (MSC) isolated from dental pulp and periodontal ligament after γ-irradiation and the effect of ataxia-telangiectasia mutated (ATM) inhibition. METHODS:MSC were irradiated with 2 and 20 Gy by (60)Co. For ATM inhibition, specific inhibitor KU5593...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2013.775530
更新日期:2013-07-01 00:00:00
abstract:PURPOSE:Ionizing radiation induces DNA damage, some of which are present in clusters, defined as two or more lesions within one to two helical turns of DNA by passage of a single radiation track. These clusters are thought to contribute to the detrimental effects of radiation, in part due to the compromised repair of c...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2014.899449
更新日期:2014-06-01 00:00:00
abstract:BACKGROUND:High dose ionizing radiation exposure is associated with myelo-depression leading to pancytopenia and the expected clinical manifestations of acute radiation syndrome (ARS). Herein, we evaluated the efficacy of sargramostim (Leukine®, yeast-derived rhu GM-CSF), with regimens delivered at 48, 72, 96, or 120 h...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2019.1673499
更新日期:2020-09-22 00:00:00
abstract::PACE (programmable, autonomously controlled electrodes) pulsed field gel electrophoresis technology was used to develop a DNA double-strand break assay that simultaneously combined (1) resolution across a broad range of DNA sizes, (2) high sensitivity (in terms of detecting dsb at low doses) and (3) speed. A 48 h PACE...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314550021
更新日期:1993-01-01 00:00:00
abstract::Radiologic accidents or terrorist acts involving radioactive material, as well as radiation exposure in medical or industrial procedures are potential sources of risk for human health. All these risks share a common element, exposure to ionizing radiation. The extent of ionizing radiation injury will depend on a numbe...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2019.1562254
更新日期:2019-05-01 00:00:00
abstract:PURPOSE:To investigate whether mobile phone radiation might affect snail nociception, employing radiofrequency (RF) electromagnetic fields (EMF) which, to our knowledge, have hitherto not been studied in a snail model. Exposure to extremely low frequency (ELF) magnetic fields has however been shown to significantly aff...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2012.644257
更新日期:2012-03-01 00:00:00
abstract:PURPOSE:A model that identifies radiation-induced genetic instability as the earliest cellular event in the multi-step sequence leading to radiation-induced cancer was previously proposed. In this paper ongoing experiments are discussed which are designed to test this model and its predictions in mouse mammary epitheli...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/095530098141023
更新日期:1998-12-01 00:00:00
abstract::The yields of strand break formation (Gssb) in single-stranded DNA (ssDNA) initiated by radiation-generated OH radicals have been determined using the method of low-angle laser light scattering (LALLS). The irradiations were carried out in aqueous, N2O-saturated solutions in the absence and presence of oxygen and at d...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009014551951
更新日期:1990-10-01 00:00:00
abstract:PURPOSE:The actinides are chemical poisons and radiological hazards. One challenge to better appraise their toxicity and develop countermeasures in case of exposure of living organisms is to better assess pathways of contamination. Because of the high chemical affinity of those actinide elements for phosphate groups an...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2014.886795
更新日期:2014-11-01 00:00:00
abstract::Purpose: To evaluate the antioxidant and radio-protective effects of Nigella sativa oil (NSO) and thymoquinone (TQ) on radiation-induced oxidative stress in brain tissue.Materials and methods: Fifty-four Sprague-Dawley rats were divided into six groups to test the radio-protective effectiveness of Nigella sativa oil a...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2020.1683636
更新日期:2020-02-01 00:00:00
abstract::Dose responses for the effects of four calcium antagonists on SCCVII/St back and leg tumour sensitivity to 20 Gy X-rays, and on tumour perfusion indicated by relative uptake of 86rubidium, were determined. The effects of these agents on 86rubidium uptake in some normal tissues were also investigated. Flunarizine sensi...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008914551511
更新日期:1989-09-01 00:00:00
abstract:PURPOSE:We already reported that levels of interferon (IFN)-γ have been shown to be markedly reduced in mice seven weeks after irradiation, resulting in a T helper (Th) 1/Th2 imbalance. To investigate whether the single or fractionated γ-irradiation induced an immune imbalance, we analysed the Th1-related immune respon...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2011.542540
更新日期:2011-05-01 00:00:00
abstract::CHEF-electrophoresis was used as a technique to detect radiation-induced DNA breakage with special emphasis to biological relevant X-ray doses (0-10 Gy). Fluorescence detection of DNA-fragments using a sensitive image analysis system was directly compared with conventional scintillation counting of 3H-thymidine prelab...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314551361
更新日期:1993-08-01 00:00:00
abstract::Photoirradiation of aqueous solutions of DNA in the presence of Al- or Zn-tetrasulphonated phthalocyanines (AIPcS4 and ZnPcS4) causes formation of strand breaks and liberation of nucleobases. The effect of added D2O, which enhances singlet oxygen (1O2) lifetime, radical scavengers including alcohols and the spin-trap ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:
更新日期:1994-12-01 00:00:00
abstract::A study has been made of the intrinsic radiosensitivity of peripheral blood lymphocytes from normal individuals. Cell survival following in vitro high (1.55 Gy min-1) and low (0.0098 Gy min-1) dose-rate irradiation was obtained for single lymphocyte samples from three individuals in six experiments. Despite wide inter...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314551571
更新日期:1993-10-01 00:00:00
abstract:UNLABELLED:Abstract Purpose: Sensitizing cancer cells to irradiation is a major challenge in clinical oncology. We aimed to define the signal transduction pathways involved in poly(ADP-ribose) polymerase (PARP) inhibitor-induced radiosensitization in various mammalian cancer lines. MATERIALS AND METHODS:Clonogenic sur...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2014.934927
更新日期:2014-12-01 00:00:00
abstract:PURPOSE:The LET position of the RBE maximum and its dependence on the cellular repair capacity was determined for carbon ions. Hamster cell lines of differing repair capacity were irradiated with monoenergetic carbon ions. RBE values for cell inactivation at different survival levels were determined and the differences...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099139232
更新日期:1999-11-01 00:00:00
abstract::The mechanisms of radiation damage production are used to examine the following premises: (1) the number of DNA double-strand breaks per unit dose increases with dose; (2) cell type to cell type variations in yield of DNA dsb per dose occur. Two stages of damage production are identified as possible sources of damage ...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553009014551251
更新日期:1990-06-01 00:00:00
abstract::Ruthenium-106 in citrate solution was administered intravenously to rat at different stages of pregnancy and to guinea-pig either before conception or in late pregnancy. The results for rat showed that retention in the embryo/foetus measured at 3-5 days after administration increased from about 0.0002% of injected act...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:
更新日期:1994-12-01 00:00:00
abstract::To characterize further the radiosensitivity of severe combined immunodeficiency (scid) mice, the induction of micronuclei (MN) in polychromatic erythrocytes as well as cell killing and translocation induction in stem cell spermatogonia was studied. Scid mice turned out to be clearly hypersensitive for X-ray-induced c...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009514550661
更新日期:1995-05-01 00:00:00
abstract:PURPOSE:To determine the contribution of intra-alveolar cells as opposed to cells fixed in the interstitium in the development of radiation-induced lung injury. MATERIALS AND METHODS:C3H/HeN mice were irradiated to the thorax with various doses of radiation. The cellular composition and cytokine production were assess...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/0955300031000076894
更新日期:2003-03-01 00:00:00
abstract::Tikvah Alper's interest in science was broad, from scrapie to mammalian cells and cancer. Much of her own work focused on cell lethality, like that of many other radiobiologists, but this was natural because of the simplicity of the endpoint cell survival and its relevance to cancer therapy. Tikvah had broader interes...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530097143662
更新日期:1997-06-01 00:00:00
abstract:PURPOSE:The molecular basis of gene regulation in cells exposed to ionising radiation is not fully understood. Gene regulation occurs at the transcriptional and post-transcriptional levels. Recent studies have suggested that micro-RNA (miRNA) plays a significant role at the post-transcriptional gene regulation. miRNA a...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553001003734568
更新日期:2010-07-01 00:00:00
abstract::Purpose: Ionizing radiation is a risk factor to the whole organism, including the heart. Cardiac damage is considered to be a late effect of radiation exposure. While the acute cardiotoxicity of high doses is well characterized, the knowledge about nature and magnitude of the cardiac risk following lower doses exposur...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2020.1704299
更新日期:2020-03-01 00:00:00
abstract::In this report, in situ hybridization with whole chromosome painting probes was used to paint radiation-induced micronuclei (MN) in three lymphoblastoid cells lines to investigate the frequency of radiation-induced MN. The results obtained for four different chromosomes showed that there was a significant deviation of...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096144860
更新日期:1996-10-01 00:00:00
abstract::Purpose: To study the response of neuronal-like cells to an applied static or low-frequency magnetic field. Materials and methods: Fourier Transform Infrared (FTIR) Spectroscopy was used to investigate the overall behavior of SH-SY5Y neuronal-like cells exposed to a static or 50 Hz magnetic fields at intensities up to...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2019.1571256
更新日期:2019-06-01 00:00:00
abstract:PURPOSE:To seek the delayed appearance of chromosomal abnormalities in human fibroblasts exposed to the Auger electron emitter 125I. MATERIALS AND METHODS:Normal untransformed human fibroblasts, HF19, were exposed to a concentration of [I125]IUdR, which allowed the survival of 37% of clonogens. Chromosomal analysis us...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000050050972
更新日期:2000-07-01 00:00:00
abstract::An iodinated DNA ligand, iodo Hoechst 33258, which binds in the minor groove of DNA, enhances DNA strand breakage and cell killing by UV-A irradiation. The sites of UV-induced strand breaks reflect the known sequence specificity of the ligand. ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009014551061
更新日期:1990-05-01 00:00:00