Abstract：
PURPOSE:Ionizing radiation induces DNA damage, some of which are present in clusters, defined as two or more lesions within one to two helical turns of DNA by passage of a single radiation track. These clusters are thought to contribute to the detrimental effects of radiation, in part due to the compromised repair of clustered DNA damaged sites. MATERIALS AND METHODS:The repair of three-lesion cluster present in oligonucleotides were determined in vitro using the hamster cell line CHO-K1 nuclear extract or purified proteins involved in base excision repair. The mutagenic potential of these clusters present in a plasmid was determined using an Escherichia coli reporter assay. RESULTS:We have shown that the repair of an abasic (AP) site within a three-lesion cluster, comprised of an AP site and bi-stranded 8-oxo-7,8-dihydroguanine (8-oxoG) lesions, is retarded compared to that of an isolated AP site in an in vitro base excision repair (BER) assay. Further, the mutation frequency of the clustered damaged site is up to three times greater than that of an isolated 8-oxoG lesion. CONCLUSIONS:As a consequence of enhanced mutagenic potential of clusters, non-double-strand break (DSB) DNA damage may contribute to the detrimental effects of radiation, in addition to the effects of DSB.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Cunniffe S,Walker A,Stabler R,O'Neill P,Lomax MEdoi
10.3109/09553002.2014.899449subject
Has Abstractpub_date
2014-06-01 00:00:00pages
468-79issue
6eissn
0955-3002issn
1362-3095journal_volume
90pub_type
杂志文章abstract：PURPOSE:Ifosfamide as a chemotherapeutic drug is used for the treatment of different cancer types. The purpose of this study is the preparation of 99mTc-ifosfamide complex to be evaluated as a potential candidate for tumor imaging. MATERIALS AND METHODS:The radiolabeling of ifosfamide with technetium-99m was carried o...
journal_title：International journal of radiation biology
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journal_title：International journal of radiation biology
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journal_title：International journal of radiation biology
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abstract：PURPOSE:Melanoma is mainly treated by surgery and rarely with radiation because of the high radioresistance of this tumor. Nevertheless, radiotherapy is the preferred treatment modality for unresectable lesions and avoiding cosmetic disfigurement caused by surgical excision. This study investigated the therapeutic adva...
journal_title：International journal of radiation biology
pub_type： 杂志文章
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更新日期：2017-05-01 00:00:00
abstract：:Studies of protein phosphorylation in the second cell cycle of mouse embryos were assessed by [32P]-incorporation and one- and two-dimensional electrophoresis. Unirradiated and X-irradiated embryos were compared. X-irradiation in the G2 phase led to a block in this cell cycle phase. The phosphorylation of proteins wit...
journal_title：International journal of radiation biology
pub_type： 杂志文章
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journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530098142617
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journal_title：International journal of radiation biology
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journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000110094805
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journal_title：International journal of radiation biology
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journal_title：International journal of radiation biology
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journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000412331283515
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journal_title：International journal of radiation biology
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doi：10.1080/09553009414550891
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pub_type： 杂志文章,收录出版
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更新日期：2019-10-01 00:00:00
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journal_title：International journal of radiation biology
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journal_title：International journal of radiation biology
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