Emp is a component of the nuclear matrix of mammalian cells and undergoes dynamic rearrangements during cell division.

abstract：:DNA cleavage by N-(O,O-diisopropyl)phosphoryl histidine(DIPP-His) is investigated by a submarine agarose electrophoresis technique. It was found that DIPP-His could cleave supercoiled DNAs and lambda DNA without the participation of divalent metal ions, while the histidine itself and N-(O,O-diisopropyl)phosphoryl alan...

journal_title：Biochemical and biophysical research communications

authors： Li Y,Sha YW,Ma Y,Zhao Y

更新日期：1995-08-24 00:00:00

abstract：:The production of hydroxyl radicals by tetrachlorohydroquinone (TCHQ, a major metabolite of the widely used biocide pentachlorophenol) in the presence of H(2)O(2) was studied by salicylate hydroxylation method. HPLC with electrochemical detection was used to measure the levels of 2,3- and 2,5-dihydroxybenzoic acid (DH...

journal_title：Biochemical and biophysical research communications

authors： Zhu BZ,Kitrossky N,Chevion M

更新日期：2000-04-21 00:00:00

abstract：:Interleukin-6 (IL-6) signal is transduced through a membrane glycoprotein gp130, which associates with the IL-6 receptor alpha chain (IL-6R alpha) in the presence of IL-6. We prepared monoclonal antibodies (mAbs) specific to murine gp130 by immunizing rats and hamsters with a chimeric molecule consisting of the extrac...

journal_title：Biochemical and biophysical research communications

authors： Matsuda T,Hirano T

更新日期：1994-07-15 00:00:00

abstract：:The reactivity of human thioredoxin (HTR) was tested in several reactions. HTR was as efficient as E. coli or plant and algal thioredoxins when assayed with E. coli ribonucleotide reductase or for the reduction of insulin. On the other hand, HTR was poorly reduced by NADPH and the E. coli flavoenzyme NADPH thioredoxin...

journal_title：Biochemical and biophysical research communications

authors： Jacquot JP,de Lamotte F,Fontecave M,Schürmann P,Decottignies P,Miginiac-Maslow M,Wollman E

更新日期：1990-12-31 00:00:00

abstract：:Lysophosphatidylcholine (lyso-PC), a biologically active phospholipid, appears to modulate various endothelial cell functions through tyrosine kinase-dependent signaling pathways. In cultured bovine aortic endothelial cells (BAEC), we have found that a 130 kDa protein (p130) was rapidly tyrosine phosphorylated within ...

journal_title：Biochemical and biophysical research communications

authors： Ochi H,Kume N,Nishi E,Moriwaki H,Masuda M,Fujiwara K,Kita T

更新日期：1998-02-24 00:00:00

abstract：:Mental status changes are usually associated with septic shock. However, the molecular mechanisms underlying physiological changes of the central nervous system (CNS) remain speculative. We have investigated the expression of interleukin (IL)-12, one of the central cytokines in inflammatory and immune responses, in th...

journal_title：Biochemical and biophysical research communications

authors： Park JH,Shin SH

更新日期：1996-07-16 00:00:00

abstract：:Osterix (Osx) is a novel zinc finger-containing transcription factor that is essential for osteoblast differentiation and bone formation in bone homeostasis. The mitogen-activated protein (MAP) kinases are a group of evolutionarily conserved proline-directed protein serine/threonine kinases that are activated in respo...

journal_title：Biochemical and biophysical research communications

authors： Choi YH,Gu YM,Oh JW,Lee KY

更新日期：2011-11-25 00:00:00

abstract：:The refolding kinetics of cobrotoxin (CBTX), a small-molecular-weight ( approximately 7 kDa) all beta-sheet protein, has been monitored using a variety of biophysical techniques. The secondary structure formation and hydrophobic collapse occur as distinct events during the refolding of the protein. Complete secondary ...

journal_title：Biochemical and biophysical research communications

authors： Sivaraman T,Kumar TK,Tu YT,Wang W,Lin WY,Chen HM,Yu C

更新日期：1999-06-24 00:00:00

abstract：:The perchloric acid-soluble protein (PSP) is an endoribonuclease and on the basis of sequence similarity has been assigned to the YjgF/YER057c/UK114 family. These family members are ubiquitous and highly conserved in evolution, and participate in regulating basic cellular metabolism. Here we present the 2.1A crystal s...

journal_title：Biochemical and biophysical research communications

authors： Zhang HM,Gao Y,Li M,Chang WR

更新日期：2010-06-18 00:00:00

abstract：:Complementary DNA corresponding to total poly(A)+-RNA from the human A431 epidermoid carcinoma cell line was cloned in the phage expression vector lambda gt 11. An epidermal growth factor (EGF) receptor cDNA clone was obtained by screening of the expression library with a rabbit polyclonal antibody (IgG), raised to th...

journal_title：Biochemical and biophysical research communications

authors： Simmen FA,Gope ML,Schulz TZ,Wright DA,Carpenter G,O'Malley BW

更新日期：1984-10-15 00:00:00

abstract：:When rat hepatoma cells (R-Y121B) were incubated with insulin at 37 degrees C, concanavalin A increased insulin internalization into cells. When R-Y121B cells were first incubated with labeled insulin at 4 degrees C then with concanavalin A at various concentrations at 37 degrees C, the total cellular radioactivity wa...

journal_title：Biochemical and biophysical research communications

authors： Sorimachi K,Yasumura Y

更新日期：1984-07-18 00:00:00

abstract：:We established a rapid procedure for obtaining transgenic mice by directly injecting an enhanced green fluorescent protein (EGFP)-expressing plasmid (pIRES-EGFP) into the ovaries of fertile mice. The frequency of transgenic mouse production was determined by pair-mating, and by polymerase chain reaction (PCR) and sequ...

journal_title：Biochemical and biophysical research communications

authors： Yang SY,Wang JG,Cui HX,Sun SG,Li Q,Gu L,Hong Y,Liu PP,Liu WQ

更新日期：2007-06-22 00:00:00

abstract：:4-Hydroxyphenylacetic acid 3-hydroxylase is a key enzyme in the pathway for the microbial degradation of phenylalanine, tyrosine and many aromatic amines. This enzyme was purified to homogeneity from Pseudomonas putida by affinity chromatography. The protein had a molecular weight of 91,000 and was a dimer of identica...

journal_title：Biochemical and biophysical research communications

authors： Raju SG,Kamath AV,Vaidyanathan CS

更新日期：1988-07-29 00:00:00

abstract：:A novel protein kinase activity was characterized from the cytosolic fraction of isolated rabbit gastric glands. The kinase phosphorylated a major 33,000 Da endogenous protein (pp33) and was stimulated by Zn2+ and Mn2+ with Kact of 1.0 and 7.5 mM, respectively. Mg2+ and Ca2+ failed to stimulate any pp33 kinase activit...

journal_title：Biochemical and biophysical research communications

authors： Goldenring JR,Oddsdottir M,Modlin IM

更新日期：1987-01-30 00:00:00

abstract：:BRAF25 is an alternatively spliced protein of BRAF35 (see associated paper). We have mapped the BRAF25 gene to chromosome sub-band 19p13.3, a region where loss of chromosomal heterozygosity has been reported in about 50% of ovarian cancers. Because of the high incidence of genetic links of prostate cancer to breast an...

journal_title：Biochemical and biophysical research communications

authors： Wang C,McCarty IM,Balazs L,Li Y,Steiner MS

更新日期：2002-07-05 00:00:00

abstract：:The number of accessible SH-groups was determined in membrane vesicles prepared from Escherichia coli growing in fermentation conditions at slightly alkaline pH on glucose with or without added formate. Addition of ATP or formate to the vesicles caused a approximately 1.4-fold increase in the number of accessible SH-g...

journal_title：Biochemical and biophysical research communications

authors： Mnatsakanyan N,Poladian A,Bagramyan K,Trchounian A

更新日期：2003-08-29 00:00:00

abstract：:A synthetic geranylgeranoic acid (GGA) induced apoptotic cell death in a human hepatoma cell line, HuH-7, but not in mouse primary cultured hepatocytes. Prior to chromatin condensation, GGA induced a dramatic loss of the mitochondrial membrane potential in 1 hour and in a dose dependent manner in HuH-7 cells, but not ...

journal_title：Biochemical and biophysical research communications

authors： Shidoji Y,Nakamura N,Moriwaki H,Muto Y

更新日期：1997-01-03 00:00:00

abstract：:Hypothetical lambda protein ORF314 shows significant homology with the carboxyl end of phage T4 tail-fiber protein gp37. Homology can also be demonstrated between hypothetical lambda protein ORF194 and a fragment of bacteriophage T4 protein gp38. This sequence homology is also reflected in the genomic sequences of the...

journal_title：Biochemical and biophysical research communications

authors： George DG,Yeh LS,Barker WC

更新日期：1983-09-30 00:00:00

abstract：:Receptor content in cultured kidney (LLC-PK1) cells was found to be modulated following the introduction of a culture medium change, declining to 40% of control values at 18 h. Scatchard analysis indicated that the reduced 1,25(OH)2-[3H]D3 nuclear binding we detected was due to decreased abundance of receptors (3811 v...

journal_title：Biochemical and biophysical research communications

authors： Hirst M,Feldman D

更新日期：1983-10-14 00:00:00

abstract：:Erythroid band 4.1 enhances the binding of erythroid spectrin to phosphatidylserine vesicles under conditions of ionic strength and at protein concentrations similar to those in the red cell. The extent of enhancement depends on the concentration of band 4.1; at 2 microM 4.1, spectrin binding increases approximately 1...

journal_title：Biochemical and biophysical research communications

authors： Takeshita K,MacDonald RI,MacDonald RC

更新日期：1993-02-26 00:00:00

abstract：:In this research, hemagglutinating virus of Japan envelope (HVJ-E) was used to reprogram somatic cells by fusion with mouse embryonic stem (ES) cells. Neomycin-resistant mouse embryonic fibroblasts (MEFs) were used as somatic cells. Nanog-overexpressing puromycin-resistant EB3 cells were used as mouse ES cells. These ...

journal_title：Biochemical and biophysical research communications

authors： Yue XS,Fujishiro M,Toyoda M,Akaike T,Ito Y

更新日期：2010-04-16 00:00:00

abstract：:Liver fibrosis and cirrhosis result from uncontrolled secretion and accumulation of extracellular matrix (ECM) proteins by hepatic stellate cells (HSCs) that are activated by liver injury and inflammation. Despite the progress in understanding the biology liver fibrogenesis and the identification of potential targets ...

journal_title：Biochemical and biophysical research communications

authors： Karthikeyan S,Potter JJ,Geschwind JF,Sur S,Hamilton JP,Vogelstein B,Kinzler KW,Mezey E,Ganapathy-Kanniappan S

更新日期：2016-01-15 00:00:00

abstract：:IL-17 plays an important role in gut homeostasis. However, the role of IL-17F in intestinal tumorigenesis has not been addressed. Here we demonstrate that ablation of IL-17F significantly inhibits spontaneous intestinal tumorigenesis in the small intestine of Apc(Min/+) mice. IL-17F ablation decreased IL-1β and Cox-2 ...

journal_title：Biochemical and biophysical research communications

authors： Chae WJ,Bothwell AL

更新日期：2011-10-14 00:00:00

abstract：:The effects of compounds which bind in the DNA minor groove of A.T rich sequences, on bleomycin-catalyzed site-specific DNA cleavage were investigated by a DNA sequencing technique. Distamycin A enhanced bleomycin-catalyzed DNA cleavage in G.C rich sequences such as 5'-GGGGC-3' (under scoring; the cleaved nucleotide)....

journal_title：Biochemical and biophysical research communications

authors： Yamamoto K,Kawanishi S

更新日期：1992-02-28 00:00:00

abstract：:cDNA microarray analyses can be used to identify candidate genes that play important roles in human carcinogenesis. To gain insight into the molecular sketch of colorectal cancer, we have constructed cDNA microarrays specialized for colorectal cancer, which we named "Colonochip" by selecting genes that are expressed i...

journal_title：Biochemical and biophysical research communications

authors： Takemasa I,Higuchi H,Yamamoto H,Sekimoto M,Tomita N,Nakamori S,Matoba R,Monden M,Matsubara K

更新日期：2001-08-03 00:00:00

abstract：:Transforming growth factor beta 1 (TGF beta 1) has been reported to be secreted and to act within the somatic cells of the testis. We examined whether the TGF beta 1 expression is present at mRNA and protein levels in purified rat Sertoli cells (SC), purified pachytene spermatocytes (SPC), and early spermatids (SPT) c...

journal_title：Biochemical and biophysical research communications

authors： Avallet O,Gomez E,Vigier M,Jégou B,Saez JM

更新日期：1997-09-29 00:00:00

abstract：:Neddylation is an ubiquitin-like modification of proteins that affects the activity, stability and protein-protein interaction of its substrates. Apart from its role as a promoter for Cullin ring E3 ligase to positively regulate the ubiquitylation process, other functional studies about neddylation are still lacking. ...

journal_title：Biochemical and biophysical research communications

authors： Li S,Fang W,Cui Y,Shi H,Chen J,Li L,Zhang L,Zhang X

更新日期：2020-09-03 00:00:00

abstract：:Different groups including ours have shown that curcumin induces melanoma cell apoptosis, here we focused the role of mammalian Sterile 20-like kinase 1 (MST1) in it. We observed that curcumin activated MST1-dependent apoptosis in cultured melanoma cells. MST1 silencing by RNA interference (RNAi) suppressed curcumin-i...

journal_title：Biochemical and biophysical research communications

authors： Yu T,Ji J,Guo YL

更新日期：2013-11-08 00:00:00

abstract：:Intestinal epithelial cells (IECs) must be tolerant of the large number of commensal bacteria inhabiting the intestinal tract to avoid excessive inflammatory reactions. Toll-interacting protein (Tollip), a negative regulator of Toll-like receptor signaling, is known to be expressed at high levels in IECs, and to there...

journal_title：Biochemical and biophysical research communications

authors： Sugi Y,Takahashi K,Nakano K,Hosono A,Kaminogawa S

更新日期：2011-09-09 00:00:00

abstract：:Human artificial chromosome (HAC) has several advantages as a gene therapy vector, including stable episomal maintenance and the ability to carry large gene inserts. Induced pluripotent stem (iPS) cells also have a great potential for gene therapy, which can be generated from an individual's own tissues and contribute...

journal_title：Biochemical and biophysical research communications

authors： Yakura Y,Ishihara C,Kurosaki H,Kazuki Y,Komatsu N,Okada Y,Doi T,Takeya H,Oshimura M

更新日期：2013-02-08 00:00:00