Abstract：
:Toll-like receptors recognize specific patterns of microbial components and regulate the activation of both innate and adaptive immunity. TLR4 recognizes lipopolysaccharide (LPS) in monocytes/macrophages with the help of other molecules like CD14 and MD-2, which indicates that the functional LPS receptor forms a large complex. The functional relationship between the components has been the subject of debate, as have the modifications induced by the ligand in the expression of some of these components. Moreover, as for other members of this family of receptors, the possible direct interaction of receptors and their ligands is a matter of discussion. In this paper we address the question of whether the expression of some of the components influences the expression of the rest. Human monocytes in which CD14 has been downregulated through interference in the turnover of the molecule at the Golgi level, show normal membrane TLR4 expression, when compared with control cells. On the other hand, LPS alters membrane TLR4 expression by monocytes devoid of membrane CD14 only in the presence of human serum. The effect of serum is blocked by anti-CD14 monoclonal antibodies, which strongly suggests a functional role for soluble CD14/LPS complexes in the interaction with TLR4. Our data add information on the relationship between the components of the LPS receptor and the characteristics of the interaction of LPS and TLR4 in cells devoid of membrane CD14.
journal_name
Microbes Infectjournal_title
Microbes and infectionauthors
Moreno C,Merino J,Ramírez N,Echeverría A,Pastor F,Sánchez-Ibarrola Adoi
10.1016/j.micinf.2004.05.010subject
Has Abstractpub_date
2004-09-01 00:00:00pages
990-5issue
11eissn
1286-4579issn
1769-714Xpii
S1286-4579(04)00188-1journal_volume
6pub_type
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journal_title：Microbes and infection
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pub_type： 杂志文章
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journal_title：Microbes and infection
pub_type： 杂志文章
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journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/j.micinf.2004.08.008
更新日期：2004-11-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/j.micinf.2007.03.004
更新日期：2007-06-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章
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journal_title：Microbes and infection
pub_type： 杂志文章,评审
doi：10.1016/j.micinf.2005.12.001
更新日期：2006-04-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/s1286-4579(02)00079-5
更新日期：2003-02-01 00:00:00
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pub_type： 杂志文章
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journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/j.micinf.2003.05.001
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journal_title：Microbes and infection
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journal_title：Microbes and infection
pub_type： 杂志文章,评审
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journal_title：Microbes and infection
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journal_title：Microbes and infection
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journal_title：Microbes and infection
pub_type： 杂志文章,评审
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journal_title：Microbes and infection
pub_type： 杂志文章,评审
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更新日期：2003-04-01 00:00:00
