Structural determinants for the action of grayanotoxin in D1 S4-S5 and D4 S4-S5 intracellular linkers of sodium channel alpha-subunits.

abstract：:Intracellular B(12) metabolism involves a B(12) trafficking chaperone CblC that is well conserved in mammals including human. The protein CblC is known to bind cyanocobalamin (CNCbl, vitamin B(12)) inducing the base-off transition and convert it into an intermediate that can be used in enzyme cofactor synthesis. The b...

journal_title：Biochemical and biophysical research communications

authors： Jeong J,Kim J

更新日期：2011-08-26 00:00:00

abstract：:We examined the mechanism of arachidonate release induced by somatostatin-14 (SS14) in CHO-K1 cells overexpressing rat hippocampal somatostatin receptor SSTR4. SSTR4 couples to pertussis toxin (PTX)-sensitive G-protein in CHO cells and does not lead to phosphoinositides breakdown or intracellular calcium ([Ca2+]i) mob...

journal_title：Biochemical and biophysical research communications

authors： Sakanaka C,Ferby I,Waga I,Bito H,Shimizu T

更新日期：1994-11-30 00:00:00

abstract：:Helical hairpins, two closely spaced helical membrane spanning segments separated by a short surface turn, are thought to be common in integral membrane proteins. Here, we study the membrane integration of a naturally occurring helical hairpin from the secretory Na(+)-K(+)-2Cl(-) cotransporter NKCC1. This sequence is ...

journal_title：Biochemical and biophysical research communications

authors： Nagy A,Turner RJ

更新日期：2007-05-04 00:00:00

abstract：:Two polypeptides are involved in interleukin 2 binding: a low-affinity receptor of 55 kD (IL2-R alpha) and an intermediate affinity component of 75 kD (IL2-R beta). We describe the cloning by the Polymerase Chain Reaction of the coding region of IL2-R alpha from a human T-cell lymphoma cell line. One clone presented a...

journal_title：Biochemical and biophysical research communications

authors： Mercken L,Moras V,Hemon L,Lionne B,Bousseau A,Dautry-Varsat A,Collins M,Mayaux JF

更新日期：1991-11-14 00:00:00

abstract：:The replication of human mitochondrial DNA (mtDNA) is initiated from a pair of displaced origins, one priming continuous synthesis of daughter-strand DNA from the heavy strand (OH) and the other priming continuous synthesis from the light strand (OL). In patients with sporadic large-scale rearrangements of mitochondri...

journal_title：Biochemical and biophysical research communications

authors： Umeda S,Tang Y,Okamoto M,Hamasaki N,Schon EA,Kang D

更新日期：2001-08-31 00:00:00

abstract：:Somatostatin exerts diverse effects in various tissues upon binding its specific membrane receptors. Recently, we have cloned three different somatostatin receptor subtypes. Here we report the sequence and functional expression of a fourth and a fifth human somatostatin receptor subtype, termed hSSTR4 and hSSTR5, resp...

journal_title：Biochemical and biophysical research communications

authors： Yamada Y,Kagimoto S,Kubota A,Yasuda K,Masuda K,Someya Y,Ihara Y,Li Q,Imura H,Seino S

更新日期：1993-09-15 00:00:00

abstract：:Exposure of [3H]-lysine labeled elastin to either purified myeloperoxidase plus H2O2 and halides or human neutrophils plus phorbol myristate acetate resulted in oxidation of lysine side chains quantitated as 3H2O release. In both the enzyme and cell system oxidation was blocked by azide, cyanide or catalase, but not b...

journal_title：Biochemical and biophysical research communications

authors： Clark RA,Szot S,Williams MA,Kagan HM

更新日期：1986-03-13 00:00:00

abstract：:The gene encoding a red-shifted green fluorescent protein variant (EGFP) was introduced into a human immunodeficiency virus type 1 (HIV-1) molecular clone by replacing the nef gene. The EGFP-expressing HIV-1 replicated efficiently in established human T cells. The expression of EGFP in the virus-infected cells was con...

journal_title：Biochemical and biophysical research communications

authors： Lee AH,Han JM,Sung YC

更新日期：1997-04-07 00:00:00

abstract：:Upon centrifugation of gently lysed T. cruzi cells through a sucrose gradient, a free DNA fraction was shown to contain catenated dimers and knotted DNA structures. Southern hybridization and electron microscopic studies indicated that both of these structures derived from minicircle DNA, the major component of T. cru...

journal_title：Biochemical and biophysical research communications

authors： Valenzuela MS,Bardhan S,Krishnamani MR,Siddiqui KA

更新日期：1991-01-31 00:00:00

abstract：:The microvesicles (MVs) in bovine posterior pituitaries contain the reserpine-sensitive vesicular monoamine transporter (Moriyama et al. (1995) J. Biol. Chem. 270, 11424-11429). An antibody against the N-terminal region of the monoamine transporter from bovine chromaffin granules recognized a polypeptide in the MVs wi...

journal_title：Biochemical and biophysical research communications

authors： Moriyama Y,Yamada H

更新日期：1996-04-25 00:00:00

abstract：:The present study demonstrates that human platelets possess a specific L-arginine transport system able to provide adequate amounts of L-arginine for endogenous nitric oxide production. L-arginine uptake takes place through a saturable high affinity carrier-mediated Na(+)-independent process which is significantly inh...

journal_title：Biochemical and biophysical research communications

authors： Vasta V,Meacci E,Farnararo M,Bruni P

更新日期：1995-01-26 00:00:00

abstract：:Self-association of amyloid beta-peptide (Abeta) is considered to be an initial step in the development of Alzheimer's disease and is known to be promoted by negatively charged lipid membranes. We have examined the possibility of non-electrostatic Abeta-membrane interaction by using neutral phosphatidylcholine lipids....

journal_title：Biochemical and biophysical research communications

authors： Yoda M,Miura T,Takeuchi H

更新日期：2008-11-07 00:00:00

abstract：:In this study, the full-length cDNA of the UDP-glucose pyrophosphorylase gene was isolated from jute by homologous cloning (primers were designed according to the sequence of UGPase gene of other plants) and modified RACE techniques; the cloned gene was designated CcUGPase. Using bioinformatic analysis, the gene was i...

journal_title：Biochemical and biophysical research communications

authors： Zhang G,Qi J,Xu J,Niu X,Zhang Y,Tao A,Zhang L,Fang P,Lin L

更新日期：2013-12-13 00:00:00

abstract：:An in organello footprinting approach has been used to probe a protein-DNA interaction of a nuclear coded 25 kDa protein, previously isolated in our laboratory, that binds "in vitro" a region within the ND2 gene, located upstream of the Ori-L. Footprinting studies with the purine-modifying reagent dimethyl sulfate and...

journal_title：Biochemical and biophysical research communications

authors： Cingolani G,Capaccio L,D'Elia D,Gadaleta G

更新日期：1997-02-24 00:00:00

abstract：:Apigenin, a plant flavonoid, induced the reversion of transformed phenotypes of v-H-ras-transformed NIH 3T3 cells at a quite low concentration of 12.5 microM. In the present study, we have examined the components of this Ras-mediated signaling transduction to determine whether they were involved in the apigenin-induce...

journal_title：Biochemical and biophysical research communications

authors： Kuo ML,Yang NC

更新日期：1995-07-26 00:00:00

abstract：:A melanin (melanosome) concentrating hormone, MCH, was synthesized and the methodology for its synthesis is detailed. This heptadecapeptide, H-Asp-Thr-Met-Arg-Cys-Met-Val-Gly-Arg-Val-Tyr-Arg-Pro-Cys-Trp-Glu-Val-OH , stimulated melanosome concentration (centripetal aggregation) within melanophores of all species of tel...

journal_title：Biochemical and biophysical research communications

authors： Wilkes BC,Hruby VJ,Sherbrooke WC,Castrucci AM,Hadley ME

更新日期：1984-07-31 00:00:00

abstract：:WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. The cloning and characterization of a novel human WW domain-containing gene, hWW45, which encodes a protein of app...

journal_title：Biochemical and biophysical research communications

authors： Valverde P

更新日期：2000-10-05 00:00:00

abstract：:Plexin-B1 regulates various cellular processes interacting directly with several Rho proteins. Molecular details of these interactions are, however, not well understood. In this study, we examined in vitro and in silico the interaction of the Rho binding domain (B1RBD) of human Plexin-B1 with 11 different Rho proteins...

journal_title：Biochemical and biophysical research communications

authors： Fansa EK,Dvorsky R,Zhang SC,Fiegen D,Ahmadian MR

更新日期：2013-05-17 00:00:00

abstract：:As a step towards the elucidation of the role played by nuclear polyphosphoinositides, we have investigated the effect of exogenous calcium free inositol (1,4,5)-trisphosphate on the in vitro phosphorylation of proteins in nuclei prepared from Swiss 3T3 cells treated with bombesin and insulin-like growth factor I. Whe...

journal_title：Biochemical and biophysical research communications

authors： Martelli AM,Gilmour RS,Manzoli FA,Cocco L

更新日期：1990-11-30 00:00:00

abstract：:A synthetic peptide, selected in the predicted N-terminal amino-acid sequence of the purine cytosine permease (gene FCY2), linked to albumins proved a remarkably good immunogen in rabbits. In ELISA, sera reacted with the synthetic peptide and with specific proteins of plasma-membrane-enriched fractions of mutant Sacch...

journal_title：Biochemical and biophysical research communications

authors： Grandier-Vazeille X,Neaud V,Geoffre S

更新日期：1993-12-15 00:00:00

abstract：:Oxygen free radicals have been implicated in the pathogenesis of ischemic cell injuries. These free radicals are normally scavenged by antioxidant enzymes. Adenosine is normally released during ischemia and protects against ischemic injuries by interacting with adenosine receptors (ARs). The mechanism underlying its c...

journal_title：Biochemical and biophysical research communications

authors： Maggirwar SB,Dhanraj DN,Somani SM,Ramkumar V

更新日期：1994-06-15 00:00:00

abstract：:AIDS patients (2 groups) had a blood deficiency (p less than 0.001) of coenzyme Q10 vs. 2 control groups. AIDS patients had a greater deficiency (p less than 0.01) than ARC patients. ARC patients had a deficiency (p less than 0.05) vs. control. HIV-infected patients had a deficiency (p less than 0.05) vs. control. The...

journal_title：Biochemical and biophysical research communications

authors： Folkers K,Langsjoen P,Nara Y,Muratsu K,Komorowski J,Richardson PC,Smith TH

更新日期：1988-06-16 00:00:00

abstract：:We have obtained two new mAbs to the carboxy-terminal region of fibronectin, namely P3D4 and P1F11, and have studied their binding sites and their ability to block lymphocyte adhesion to fibronectin. ELISA and Western blot analyses showed that P3D4 reacts with both fibronectin chains and both Hep II-containing fragmen...

journal_title：Biochemical and biophysical research communications

authors： Garcia-Pardo A,Sánchez-Aparicio P,Wayner EA

更新日期：1992-07-15 00:00:00

abstract：:Th2 cell differentiation is critically influenced by transcription factor GATA-3 and by various cis-acting elements including enhancers, silencers and a locus control region (LCR) in the Th2 cytokine locus. Th2 LCR-deficient Th2 cells completely lost the expression of GATA-3 and the phosphorylation of STAT6. Histone 3...

journal_title：Biochemical and biophysical research communications

authors： Hwang SS,Kim K,Lee GR

更新日期：2011-07-15 00:00:00

abstract：:The biosynthesis of the anticancer drug Taxol in yew (Taxus) species is thought to involve the preliminary formation of the advanced taxane diterpenoid intermediate baccatin III upon which the functionally important N-benzoyl phenylisoserinoyl side chain is subsequently assembled at the C13-O-position. In vivo feeding...

journal_title：Biochemical and biophysical research communications

authors： Long RM,Croteau R

更新日期：2005-12-09 00:00:00

abstract：:Alanine racemase (Alr) is a pyridoxal-5'-phosphate-dependent (PLP) enzyme that catalyzes a reversible racemization between the enantiomers of alanine. d-Alanine is an indispensable constituent in the biosynthesis of bacterial cell-wall peptidoglycan, and its inhibition is lethal to prokaryotes, which makes it an attra...

journal_title：Biochemical and biophysical research communications

authors： Dong H,Hu T,He G,Lu D,Qi J,Dou Y,Long W,He X,Ju J,Su D

更新日期：2018-02-26 00:00:00

abstract：:Insect midgut peritrophic membrane (PM) is a functional structure that protects insects against chemical damage and microorganism infection. The essential component in PM is chitin and its synthesis is catalyzed by Class B chitin synthase (CHSB), which plays a unique role in chitin-containing organisms and thus repres...

journal_title：Biochemical and biophysical research communications

authors： Qu M,Liu T,Yang J,Yang Q

更新日期：2011-01-07 00:00:00

abstract：:Chloride intracellular channel protein CLIC4 is a putative organellar anion channel or channel regulator with an unusual dual cytoplasmic and integral membrane localisation. To investigate its contribution to cellular anion channel activity, the protein was overexpressed in stably transfected HEK-293 cells. Patch-clam...

journal_title：Biochemical and biophysical research communications

authors： Proutski I,Karoulias N,Ashley RH

更新日期：2002-09-20 00:00:00

abstract：:Rabbit antibodies have been raised against rat liver nuclear envelopes. An enzyme-linked immunosorbent assay (ELISA) demonstrated high titer antiserum specific for the nuclear envelope preparation. Immunocytochemical studies showed that the antiserum stained the nuclear envelopes, but not intra-nuclear components of H...

journal_title：Biochemical and biophysical research communications

authors： Venkatraman JT,Gohill J,Fritzler MJ,Lefebvre YA

更新日期：1988-10-31 00:00:00

abstract：:Iron is required in all organisms for crucial functions, as a number of proteins need iron for activity. Mutations of the genes encoding proteins involved in iron uptake, transport, and utilization result in various human disorders or animal models with very different clinical presentations and organ involvement. Howe...

journal_title：Biochemical and biophysical research communications

authors： Rötig A,Chantrel-Groussard K,Munnich A,Rustin P

更新日期：2001-03-02 00:00:00