当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Reversion of v-H-ras-transformed NIH 3T3 cells by apigenin through inhibiting mitogen activated protein kinase and its downstream oncogenes.

Reversion of v-H-ras-transformed NIH 3T3 cells by apigenin through inhibiting mitogen activated protein kinase and its downstream oncogenes.

Abstract：

:Apigenin, a plant flavonoid, induced the reversion of transformed phenotypes of v-H-ras-transformed NIH 3T3 cells at a quite low concentration of 12.5 microM. In the present study, we have examined the components of this Ras-mediated signaling transduction to determine whether they were involved in the apigenin-induced reversion process. Interestingly, the consitutively activated mitogen activated protein kinase (MAPK) in the ras transformant was inhibited significantly and rapidly by 25 microM apigenin within 30 min, and this reduction continued for more than 4 h. Corroborating these observations, expression of the downstream oncogenes c-jun and c-fos was also dramatically reduced during the first 4 h of treatment. We found that the levels of ras protein and mRNA were not affected by 24 h of treatment with apigenin. These findings indicate that apigenin-induced reversion of v-H-ras-transformed NIH 3T3 cells may occur by inhibiting MAPK activity and its downstream oncogenes rather than by affecting the expression of the ras gene.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Kuo ML,Yang NC

doi

10.1006/bbrc.1995.2035

subject

Has Abstract

pub_date

1995-07-26 00:00:00

pages

767-75

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006291X85720359

journal_volume

212

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Transduction of anti-apoptotic proteins into chondrocytes in cartilage slice culture.

    abstract：:Peptides of the protein transduction domain (PTD) mediate the introduction of passenger proteins into cells in vitro and in vivo, where the domains are positively charged. This unusual ability can be exploited for medical applications in protein therapeutics. Chondrocytes are embedded in a dense extracellular matrix, ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.11.144

    authors： Ozaki D,Sudo K,Asoh S,Yamagata K,Ito H,Ohta S

    更新日期：2004-01-16 00:00:00

  • Overexpression of stearoyl-CoA desaturase-1 results in an increase of conjugated linoleic acid (CLA) and n-7 fatty acids in 293 cells.

    abstract：:The effect of human SCD1 heterologous expression on cellular fatty acid synthesis was investigated in the current study. The SCD1 gene expression cassette and PGK-neomycin-selectable marker cassette were co-introduced into HEK 293 cells by electroporation, and subsequently, SCD1 expression was evaluated by fatty acid ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.06.102

    authors： Wu Z,Li D,Gou K

    更新日期：2010-07-30 00:00:00

  • Pre-clinical evaluation of cinobufotalin as a potential anti-lung cancer agent.

    abstract：:Lung cancer is a major cause of cancer-related mortality in the United States and around the world. Due to the pre-existing or acquired chemo-resistance, the current standard chemotherapy regimens only show moderate activity against lung cancer. In the current study, we explored the potential anti-lung cancer activity...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.08.147

    authors： Kai S,Lu JH,Hui PP,Zhao H

    更新日期：2014-09-26 00:00:00

  • Molecular mechanism of an adverse drug-drug interaction of allopurinol and furosemide in gout treatment.

    abstract：:Gout patients receiving a combination of allopurinol and furosemide require higher allopurinol doses to achieve the target serum urate (SU) of <6 mg/dl (Stamp et al., 2012) [1]. Our study aimed to identify the molecular basis for this observation. We used a fluorimetric assay to determine the impact of furosemide and ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.08.068

    authors： Knake C,Stamp L,Bahn A

    更新日期：2014-09-12 00:00:00

  • Differences in the betaC-S lyase activities of viridans group streptococci.

    abstract：:betaC-S Lyase catalyzes the alpha,beta-elimination of L-cysteine to hydrogen sulfide, which is one of the main causes of oral malodor and is highly toxic to mammalian cells. We evaluated the capacity of six species of oral streptococci to produce hydrogen sulfide. The crude enzyme extract from Streptococcus anginosus ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)02803-6

    authors： Yoshida Y,Negishi M,Amano A,Oho T,Nakano Y

    更新日期：2003-01-03 00:00:00

  • Biochemical characterization of the phagocytic giant cell receptor for Dictyostelium discoideum amoebae: identification by cell blotting.

    abstract：:A critical aspect of sexual development in Dictyostelium is the selection of "self" as a targeted food source by the phagocytic zygotic giant cell (ZGC). It has previously been demonstrated that phagocytic giant cells preferentially take up amoebae of the same species suggesting that the process is mediated by a speci...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.5990

    authors： Lewis KE,Jamieson JC

    更新日期：1997-01-23 00:00:00

  • Evidence for an inactive plasma membrane-associated precursor of active cytoplasmic ornithine decarboxylase in developing embryos of Musca domestica.

    abstract：:During embryonic development of Musca domestica inactive ornithine decarboxylase protein appears in the embryos at 6 h postoviposition, increases in concentration and reaches a maximum level at 9 h postoviposition. The inactive enzyme is associated with the plasma membrane and appears to be the precursor for active or...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)91444-h

    authors： Lilleberg SL,Killilea SD,Vaske DA,Leopold RA,Sparks RB Jr

    更新日期：1991-01-31 00:00:00

  • Fetuin-A (α2HS-glycoprotein) is a serum chemo-attractant that also promotes invasion of tumor cells through Matrigel.

    abstract：:The present study was conducted to determine whether fetuin-A, a dominant serum protein plays a role in chemo-attraction and chemo-invasion of carcinoma cells in vitro. Serum is normally used as positive chemotaxis control in Boyden chamber motility assays, prompting the need to identify the factor/s in serum that con...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.07.125

    authors： Nangami GN,Watson K,Parker-Johnson K,Okereke KO,Sakwe A,Thompson P,Frimpong N,Ochieng J

    更新日期：2013-09-06 00:00:00

  • Tumor necrosis factor-alpha increased the integrin alpha 2 beta 1 expression and cell attachment to type I collagen in human dermal fibroblasts.

    abstract：:Cell adhesion molecules of human dermal fibroblasts play an important role in the processes of wound healing. The effects of tumor necrosis factor- alpha (TNF) on the expression of integrin beta 1 subfamily in human dermal fibroblasts were examined. TNF preferentially induced the expression of alpha 2 beta 1 integrins...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1411

    authors： Ezoe K,Horikoshi T

    更新日期：1993-04-15 00:00:00

  • Dimerization of presenilin-1 in vivo: suggestion of novel regulatory mechanisms leading to higher order complexes.

    abstract：:A growing body of evidence indicates that presenilins could exist and be active as oligomeric complexes. Using yeast two-hybrid and cell culture analysis, we provide evidence that presenilin-1 (PS1) may self-oligomerize giving rise to specific full-length/full-length homodimers. When expressed in N2A and HEK239T cultu...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)02984-4

    authors： Hébert SS,Godin C,Tomiyama T,Mori H,Lévesque G

    更新日期：2003-01-31 00:00:00

  • Computing the binding affinity of a ligand buried deep inside a protein with the hybrid steered molecular dynamics.

    abstract：:Computing the ligand-protein binding affinity (or the Gibbs free energy) with chemical accuracy has long been a challenge for which many methods/approaches have been developed and refined with various successful applications. False positives and, even more harmful, false negatives have been and still are a common occu...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.12.165

    authors： Villarreal OD,Yu L,Rodriguez RA,Chen LY

    更新日期：2017-01-29 00:00:00

  • Diethanolamine inhibits choline uptake and phosphatidylcholine synthesis in Chinese hamster ovary cells.

    abstract：:Diethanolamine (DEA), an alkanolamine used widely in industry, is hepatocarcinogenic in mice. The goal of this work was to determine whether DEA altered choline homeostasis in cultured cells, so as to ascertain whether the liver tumor response may be related to choline deficiency. CHO cells were cultured in Ham's F-12...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1253

    authors： Lehman-McKeeman LD,Gamsky EA

    更新日期：1999-09-07 00:00:00

  • Selective inactivation of MAO-B by benzyl-dimethyl-silyl-methanamines in vitro.

    abstract：:The alpha-silyl amines benzyl-dimethyl-silyl-methanamine and the p-fluoro and p-chloro derivatives are potent time-dependent inhibitors of rat brain MAO-B. The inhibition exhibits saturation kinetics, takes place in the enzyme active-site and is irreversible. The most potent inhibitor in the series is 4-fluorobenzyl-d...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)92466-2

    authors： Danzin C,Collard JN,Marchal P,Schirlin D

    更新日期：1989-04-28 00:00:00

  • Deuterium distribution in lactate as tracer of metabolic pathways.

    abstract：:2H-NMR spectroscopy has been used to study the distribution of 2H atoms in brain lactate, after intraperitoneal injection of 2H2O. Information on brain metabolism was obtained from the ratio [(3-2H) lactate]/[(2-2H)-lactate] which was found very sensitive to the PO2 in the breathing air. The dependence of this ratio o...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7208

    authors： Vianello F,Scarpa M,Viglino P,Rigo A

    更新日期：1997-08-28 00:00:00

  • Impaired adult hippocampal neurogenesis and its partial reversal by chronic treatment of fluoxetine in a mouse model of Angelman syndrome.

    abstract：:Angelman syndrome (AS) is a neurodevelopmental disorder characterized by severe cognitive and motor deficits, caused by the loss of function of maternally inherited Ube3a. Ube3a-maternal deficient mice (AS model mice) recapitulate many essential features of AS, but how the deficiency of Ube3a lead to such behavioural ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.07.103

    authors： Godavarthi SK,Dey P,Sharma A,Jana NR

    更新日期：2015-09-04 00:00:00

  • A specific, photolabile and irreversible antagonist (L662,025) of the PAF-receptor.

    abstract：:PAF (0.2 microM) induced maximal platelet aggregation in human PRP and [3H]-PAF (1-5 nM) binding to platelet membrane preparations had Kd value of 3.8 nM and Bmax of 200 fmoles/mg of protein. Without UV irradiation, a synthetic azido tetrahydrofuran derivative L662,025 was a reversible and competitive PAF-receptor ant...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)91554-4

    authors： Hussaini IM,Shen TY

    更新日期：1989-05-30 00:00:00

  • Molecular determinants of the interactions between LXR/RXR heterodimers and TRAP220.

    abstract：:Dimerization-induced activation of LXR is a unique allosteric mechanism described only for LXR/RXR heterodimers. Previously, we demonstrated that RXR functions as an allosteric activator of LXR binding to ASC-2 coactivator rather than as a direct interaction partner. Here, we investigated the molecular basis of the in...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.04.131

    authors： Son YL,Lee YC

    更新日期：2009-07-03 00:00:00

  • PprI: a general switch responsible for extreme radioresistance of Deinococcus radiodurans.

    abstract：:Deinococcus radiodurans exhibits an extraordinary ability to withstand the lethal and mutagenic effects of DNA damaging agents, particularly, ionizing radiation. Available evidence indicates that efficient repair of DNA damage and protection of the chromosomal structure are mainly responsible for the radioresistance. ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(03)00965-3

    authors： Hua Y,Narumi I,Gao G,Tian B,Satoh K,Kitayama S,Shen B

    更新日期：2003-06-27 00:00:00

  • Hrp3, a chromodomain helicase/ATPase DNA binding protein, is required for heterochromatin silencing in fission yeast.

    abstract：:Hrp3, a paralog of Hrp1, is a novel member of the CHD1 (chromo-helicase/ATPase-DNA binding 1) protein family of Schizosaccharomyces pombe. Although it has been considered that CHD1 proteins are required for chromatin modifications in transcriptional regulations, little is known about their roles in vivo. In this study...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)00797-0

    authors： Jae Yoo E,Kyu Jang Y,Ae Lee M,Bjerling P,Bum Kim J,Ekwall K,Hyun Seong R,Dai Park S

    更新日期：2002-07-26 00:00:00

  • SDF-1/CXCR4 axis promotes the growth and sphere formation of hypoxic breast cancer SP cells by c-Jun/ABCG2 pathway.

    abstract：:ATP-binding cassette sub-family G member 2 (ABCG2) confers to the major phenotypes of side population (SP) cells, the cancer stem-like cells. In this study, the SP cells displayed a distinctly higher ABCG2 expression level, sphere formation efficiency (SFE) and growth rate even under hypoxia condition. CXCR4 overexpre...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.09.130

    authors： He C,Zhang H,Wang B,He J,Ge G

    更新日期：2018-10-28 00:00:00

  • Assay of detergents by rocket electrophoresis in agarose gels containing red blood cells: "rocket hemolysis".

    abstract：:A method is described for quantitation of charged detergents using their hemolytic property in an electrophoresis assay in agarose gels containing red blood cells. After electrophoresis the zone of hemolysis is directly proportional to the concentration of detergent in the sample. Using this technique we have determin...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)90837-9

    authors： Sundquist B,Dalsgaard K,Morein B

    更新日期：1983-07-29 00:00:00

  • Casein kinase II is required for the spindle assembly checkpoint by regulating Mad2p in fission yeast.

    abstract：:The spindle checkpoint is a surveillance mechanism that ensures the fidelity of chromosome segregation in mitosis. Here we show that fission yeast casein kinase II (CK2) is required for this checkpoint function. In the CK2 mutants mitosis occurs in the presence of a spindle defect, and the spindle checkpoint protein M...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.08.030

    authors： Shimada M,Yamamoto A,Murakami-Tonami Y,Nakanishi M,Yoshida T,Aiba H,Murakami H

    更新日期：2009-10-23 00:00:00

  • Oxidative DNA damage by minor metabolites of toluene may lead to carcinogenesis and reproductive dysfunction.

    abstract：:Recently, the concern that toluene might have carcinogenic and reproductive toxic potential has been raised. We investigated the ability of DNA damage by minor metabolites of toluene, methylhydroquinone, and methylbenzoquinone, using (32)P-5'-end-labeled DNA fragments obtained from the human genes. Methylhydroquinone ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1041

    authors： Murata M,Tsujikawa M,Kawanishi S

    更新日期：1999-08-02 00:00:00

  • Head/tail interaction of vinculin influences cell mechanical behavior.

    abstract：:This study evaluates the influence of vinculin in closed conformation on the mechanical properties of cells. We demonstrate that MEFvin(-/-) cells transfected with the eGFP-vinculin mutant A50I (talin-binding-deficient-vinculin in a constitutively closed conformation) show 2-fold lower stiffness and focal adhesion den...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.01.115

    authors： Diez G,Auernheimer V,Fabry B,Goldmann WH

    更新日期：2011-03-04 00:00:00

  • Pharmacological inhibition of Rac1 exerts a protective role in ischemia/reperfusion-induced renal fibrosis.

    abstract：:Acute kidney injury induced by renal ischemia-reperfusion (IR) is a prominent risk factor in the development towards renal fibrosis. Ras-related C3 botulinum toxin substrate 1(Rac1) has been involved in the pathophysiology of fibrotic disorders. But the role of Rac1 in the pathogenesis of IR-induced renal fibrosis is ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.07.009

    authors： Liang H,Huang J,Huang Q,Xie YC,Liu HZ,Wang HB

    更新日期：2018-09-18 00:00:00

  • Molecular cloning and characterization of human FGF-20 on chromosome 8p21.3-p22.

    abstract：:The fibroblast growth factors (FGFs) play important roles in morphogenesis, angiogenesis, tissue remodeling, and carcinogenesis. Human FGF-20 has been cloned and characterized in this study. FGF-20 encodes a 211-amino-acid polypeptide with the FGF-core domain. A strong hydrophobic region was found in the FGF-core doma...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.3142

    authors： Kirikoshi H,Sagara N,Saitoh T,Tanaka K,Sekihara H,Shiokawa K,Katoh M

    更新日期：2000-08-02 00:00:00

  • Characterization and in vitro interaction study of a [NiFe] hydrogenase large subunit from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1.

    abstract：:The large subunit of the [NiFe] hydrogenases harbors a NiFe(CN)(2)(CO) cluster. Maturation proteins HypA, B, C, D, E, and F are required for the NiFe cluster biosynthesis. While the maturation machinery has been hitherto studied intensively, little is known about interactions between the Hyp proteins and the large sub...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.11.083

    authors： Sasaki D,Watanabe S,Kanai T,Atomi H,Imanaka T,Miki K

    更新日期：2012-01-06 00:00:00

  • Role of cholesterol in ligand binding and G-protein coupling of serotonin1A receptors solubilized from bovine hippocampus.

    abstract：:The serotonin(1A) (5-HT(1A)) receptor is an important member of the superfamily of seven transmembrane domain G-protein-coupled receptors. We report here that solubilization of the hippocampal 5-HT(1A) receptor by the zwitterionic detergent CHAPS is accompanied by loss of membrane cholesterol which results in a reduct...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.12.102

    authors： Chattopadhyay A,Jafurulla M,Kalipatnapu S,Pucadyil TJ,Harikumar KG

    更新日期：2005-02-25 00:00:00

  • Experimental considerations on the measurement of prostaglandins during long-time incubations of neonatal mouse calvaria.

    abstract：:The influence of experimental conditions during long-time (72 h) incubations of neonatal mouse calvaria on the measurement of prostaglandins was investigated. Incubations of the cultured calvaria were carried out in the presence and absence of stimulating agents of bone resorption, such as thrombin and parathyroid hor...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)90365-t

    authors： Leis HJ,Malle E,Hoffmann O,Klaushofer K,Fratzl-Zelman N,Koller K,Peterlik M

    更新日期：1990-06-15 00:00:00

  • Wnt-1 promotes neuronal differentiation and inhibits gliogenesis in P19 cells.

    abstract：:Wnt-1, the vertebrate counterpart of the Drosophila wingless gene, plays an important role in the early morphogenesis of neural tissues. In this report, we have shown that overexpression of Wnt-1 can direct embryonic carcinoma P19 cells to differentiate into neuron-like cells in the absence of retinoic acid. Immunocyt...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/S0006-291X(02)00215-2

    authors： Tang K,Yang J,Gao X,Wang C,Liu L,Kitani H,Atsumi T,Jing N

    更新日期：2002-04-26 00:00:00