当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Transduction of anti-apoptotic proteins into chondrocytes in cartilage slice culture.

Transduction of anti-apoptotic proteins into chondrocytes in cartilage slice culture.

Abstract：

:Peptides of the protein transduction domain (PTD) mediate the introduction of passenger proteins into cells in vitro and in vivo, where the domains are positively charged. This unusual ability can be exploited for medical applications in protein therapeutics. Chondrocytes are embedded in a dense extracellular matrix, whose components are highly negatively charged. We examined whether PTD mediates the delivery of functional proteins into chondrocytes through the matrix using the super anti-apoptotic protein FNK fused with Tat/PTD peptide (PTD-FNK), the FNK protein being constructed from anti-apoptotic Bcl-xL to enhance its activity. The PTD-FNK protein labeled with a fluorescent dye was incorporated into chondrocytes through the matrix and immunostaining confirmed the transduction into the cells. The PTD-FNK protein protected chondrocytes from cell death induced by Fas antibody and nitrogen oxide (NO). Thus, the PTD peptide has the ability to deliver passenger proteins into chondrocytes by penetrating the extracellular matrix of cartilage.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Ozaki D,Sudo K,Asoh S,Yamagata K,Ito H,Ohta S

doi

10.1016/j.bbrc.2003.11.144

subject

Has Abstract

pub_date

2004-01-16 00:00:00

pages

522-7

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006291X03025385

journal_volume

313

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • TGF-beta1 induced MMP-9 expression in HNSCC cell lines via Smad/MLCK pathway.

    abstract：:Matrix metalloproteinase-9 (MMP-9) plays roles in cancer progression by degrading the extracellular matrix and basement membrane. Many growth factors including Transforming growth factor-beta1 (TGF-beta1) could induce MMP-9 expression. We demonstrated that TGF-beta1 induced MMP-9 mRNA and protein in human head and nec...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.04.128

    authors： Sinpitaksakul SN,Pimkhaokham A,Sanchavanakit N,Pavasant P

    更新日期：2008-07-11 00:00:00

  • Tyrosine residues 239 and 240 of Shc are phosphatidylinositol 4,5-bisphosphate-dependent phosphorylation sites by c-Src.

    abstract：:In the previous study (Sato K.-I. et al. (1997) FEBS Lett. 410, 136-140), we showed that the phosphorylation of Shc protein by c-Src is dependent on the binding of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) to the PTB domain of Shc. In this study, we demonstrate that, in contrast to c-Src, v-Src and epiderm...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7667

    authors： Sato K,Gotoh N,Otsuki T,Kakumoto M,Aoto M,Tokmakov AA,Shibuya M,Fukami Y

    更新日期：1997-11-17 00:00:00

  • Protein-lipid interactions and enzyme requirements for light subtype generation on cycling reconstituted surfactant.

    abstract：:Surfactant convertase is required for conversion of heavy density (H) natural surfactant to light density (L) subtype during cycling in vitro, a technique that reproduces surfactant metabolism. To study mechanisms of H to L conversion, we prepared liposomes of dipalmitoylphosphatidylcholine (DPPC) and phosphatidylglyc...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8325

    authors： Dhand R,Sharma VK,Teng AL,Krishnasamy S,Gross NJ

    更新日期：1998-03-27 00:00:00

  • Frequent occurrence of short complementary sequences in nucleic acids.

    abstract：:The hypothesis, that nucleic acids which code specifically interacting receptor and ligand proteins contain complementary sequences was tested. Human insulin mRNA (HSINSU) contained 16 sequences which were 23.8 +/- 1.4 nucleotides long and were complementary to the insulin receptor mRNA (HSIRPR, 74.8 +/- 1.9% compleme...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(86)80084-5

    authors： Segerstéen U,Nordgren H,Biro JC

    更新日期：1986-08-29 00:00:00

  • Identification of an arginine vasopressin-like diuretic hormone from Locusta migratoria.

    abstract：:We have identified two neuropeptides (F1 and F2) from suboesophageal and thoracic ganglia of Locusta migratoria, which we isolated earlier based on their immunological similarity to arginine vasopressin. The more abundant and hydrophilic factor, F1, has sequence Cys-Leu-Ile-Thr-Asn-Cys-Pro-Arg-Gly-NH2, but its biologi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)91621-4

    authors： Proux JP,Miller CA,Li JP,Carney RL,Girardie A,Delaage M,Schooley DA

    更新日期：1987-11-30 00:00:00

  • Angiotensin II induces focal adhesion kinase/paxillin phosphorylation and cell migration in human umbilical vein endothelial cells.

    abstract：:In the present study, we demonstrated that Ang II provokes a transitory enhancement of focal adhesion kinase (FAK) and paxillin phosphorylation in human umbilical endothelial cells (HUVEC). Moreover, Ang II induces a time- and dose-dependent augmentation in cell migration, but does not affect HUVEC proliferation. The ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.12.110

    authors： Montiel M,de la Blanca EP,Jiménez E

    更新日期：2005-02-25 00:00:00

  • The soluble metalloendoprotease required in myoblast fusion remains intracellular.

    abstract：:The fusion of myoblasts to myotubes requires an endogenous soluble metalloendoprotease. To determine whether this protease is released by fusing myoblasts, or stays within the cell, we examined the effects of membrane-impermeant and a membrane-permeant metalloendoprotease inhibitors. Membrane-permeant 1,10-phenanthrol...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(87)80145-6

    authors： Farach HA Jr,Strittmatter WJ

    更新日期：1987-08-31 00:00:00

  • Ca(2+)-dependent activation of phospholipases C and D from mouse peritoneal macrophages by a selective trigger of Ca2+ influx, gamma-hexachlorocyclohexane.

    abstract：:The gamma-isomer of hexachlorocyclohexane (gamma-HCCH), which displays structural homology with inositol, was found to induce an initial influx of Ca2+ in mouse peritoneal macrophages. This was responsible for Ca(2+)-induced Ca2+ release via inositol 1,4,5-trisphosphate produced by phospholipase C and resulted in a su...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.1285

    authors： Pinelli E,Cambon C,Tronchère H,Chap H,Teissié J,Pipy B

    更新日期：1994-03-15 00:00:00

  • Novel fluorescent protein from Hydnophora rigida possess cyano emission.

    abstract：:Currently, a broad diversity of fluorescent proteins among marine organisms range from cyano-red emissions. Fluorescent proteins differ in their DNA sequences from green fluorescent protein (GFP). We identified cDNA encoding the gene of a new protein from the reef coral Hydnophora rigida of the Merulinidae family. Bot...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.04.136

    authors： Bokhari H,Smith C,Veerendra K,Sivaraman J,Sikaroodi M,Gillevet P

    更新日期：2010-06-04 00:00:00

  • pp120, a substrate of the insulin receptor tyrosine kinase, is associated with phosphatase activity.

    abstract：:pp120, a plasma membrane glycoprotein, is phosphorylated on Tyr488 by the insulin receptor tyrosine kinase. This requires basal phosphorylation on Ser503 by cAMP-dependent serine kinase. Phe513, the other tyrosine residue in the intracellular domain, does not undergo insulin-stimulated phosphorylation. Phe488 mutation...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8822

    authors： Najjar SM

    更新日期：1998-06-18 00:00:00

  • An integrated RNA-Seq and network study reveals a complex regulation process of rice embryo during seed germination.

    abstract：:Seed germination is a crucial stage for plant development and agricultural production. To investigate its complex regulation process, the RNA-Seq study of rice embryo was conducted at three time points of 0, 12 and 48 h post imbibition (HPI). Dynamic transcriptional alterations were observed, especially in the early s...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.06.110

    authors： Wei T,He Z,Tan X,Liu X,Yuan X,Luo Y,Hu S

    更新日期：2015-08-14 00:00:00

  • Synthesis, discovery and mechanism of 2,6-dimethoxy-N-(4-methoxyphenyl)benzamide as potent depigmenting agent in the skin.

    abstract：:In this study, a new skin-depigmenting agent, 2,6-dimethoxy-N-(4-methoxyphenyl)benzamide (DMPB), was synthesized using a combination of benzoic acid and aniline. DMPB exhibited significant depigmentation ability on the UV B-induced hyperpigmentation of the brown guinea pig skin. In addition, the 100ppm treatment with ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.07.206

    authors： Choi SY,Hwang JS,Kim S,Kim SY

    更新日期：2006-10-13 00:00:00

  • Cholecystokinin-induced redistribution of paxillin in rat pancreatic acinar cells.

    abstract：:Cholecystokinin (CCK) dose-dependently stimulates enzyme secretion or loss of cell integrity in the exocrine pancreas. Signaling mechanims include tyrosine phosphorylation of p125(FAK) and paxillin. Here, we examine their potential function. Maximum phosphorylation of both proteins was observed after stimulation of fr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.9413

    authors： Leser J,Lührs H,Beil MF,Adler G,Lutz MP

    更新日期：1999-01-19 00:00:00

  • Adhesion of mouse hepatocytes to type I collagen: role of supramolecular forms and effect of proteolytic degradation.

    abstract：:Mouse hepatocytes were found to adhere much stronger to intact native type I collagen than to native, telopeptide-devoid collagen. Removal of telopeptides (by pepsin treatment) caused disintegration of supramolecular collagen aggregates. Similar differences in hepatocyte adhesion were observed between oligomeric and m...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1827

    authors： Berman A,Morozevich G,Karmansky I,Gleiberman A,Bychkova V

    更新日期：1993-07-15 00:00:00

  • 18O labeling on Ser45 but not on Ser35 supports the cooperative phosphorylation mechanism on tarantula thick filament activation.

    abstract：:Thick filaments from some striated muscles are regulated by phosphorylation of myosin regulatory light chains (RLCs). A tarantula thick filament quasi-atomic model achieved by cryo-electron microscopy has advanced our understanding on how this regulation occurs. In native thick filaments, an asymmetric intramolecular ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.01.044

    authors： Sulbarán G,Biasutto A,Méndez F,Pinto A,Alamo L,Padrón R

    更新日期：2020-03-26 00:00:00

  • Distribution and characterization of immunoreactive prolactin-releasing peptide (PrRP) in rat tissue and plasma.

    abstract：:We established a sensitive and specific two-site enzyme immunoassay (EIA) for prolactin-releasing peptide (PrRP) using two region-specific monoclonal antibodies. We investigated the tissue distribution and the plasma concentration of immunoreactive (ir-) PrRP in rats using this assay. Ir-PrRP was widely distributed in...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.0463

    authors： Matsumoto H,Murakami Y,Horikoshi Y,Noguchi J,Habata Y,Kitada C,Hinuma S,Onda H,Fujino M

    更新日期：1999-04-13 00:00:00

  • Inhibition of the de-myelinating properties of Aicardi-Goutières syndrome lymphocytes by cathepsin D silencing.

    abstract：:Molecular mechanisms relating interferon-alpha (IFN-alpha) to brain damage have recently been identified in a microarray analysis of cerebrospinal fluid lymphocytes from patients with Aicardi-Goutières Syndrome (AGS). These findings demonstrate that the inhibition of angiogenesis and the activation of neurotoxic lymph...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.11.131

    authors： Pulliero A,Marengo B,Longobardi M,Fazzi E,Orcesi S,Olivieri I,Cereda C,Domenicotti C,Balottin U,Izzotti A

    更新日期：2013-01-18 00:00:00

  • Mutual suppression of miR-125a and Lin28b in human hepatocellular carcinoma cells.

    abstract：:MicroRNA-125a exhibits an antiproliferative activity and is downregulated in several types of tumors, including hepatocellular carcinoma where it targets sirtuin-7, matrix metalloproteinase-11, and c-Raf. Another target of miR-125a is Lin28, a pluripotency factor that is generally undetectable in differentiated cells ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.04.167

    authors： Panella M,Mosca N,Di Palo A,Potenza N,Russo A

    更新日期：2018-06-07 00:00:00

  • Inhibitory selectivity of canecystatin: a recombinant cysteine peptidase inhibitor from sugarcane.

    abstract：:The cDNA of a cystein peptidase inhibitor was isolated from sugarcane and expressed in Escherichia coli. The protein, named canecystatin, has previously been shown to exert antifungal activity on the filamentous fungus Trichoderma reesei. Herein, the inhibitory specificity of canecystatin was further characterized. It...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.06.053

    authors： Oliva ML,Carmona AK,Andrade SS,Cotrin SS,Soares-Costa A,Henrique-Silva F

    更新日期：2004-08-06 00:00:00

  • Molecular cloning of a family of plant genes encoding a protein homologous to plasma membrane H+-translocating ATPases.

    abstract：:cDNA clones from Nicotiana plumbaginifolia have been isolated by hybridization to a yeast H+-ATPase gene. The largest one encodes a polypeptide (PMA2) of 956 amino acid residues which exhibits a homology of 73% with a limited protein sequence obtained from purified oat plasma membrane H+-ATPase (Schaller and Sussman, ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)92348-6

    authors： Boutry M,Michelet B,Goffeau A

    更新日期：1989-07-31 00:00:00

  • Cloning and functional expression of a swelling-induced chloride conductance regulatory protein, plCln, from rabbit ocular ciliary epithelium.

    abstract：:The cDNA encoding a swelling-induced chloride conductance regulatory protein, plcln, was cloned from rabbit ciliary epithelium by using a polymerase chain reaction (PCR)-based approach. The open reading frame encoding 236 amino acids possesses high amino acid identity (93/%) with the previously cloned plcln from human...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7523

    authors： Wan XL,Chen S,Sears M

    更新日期：1997-10-29 00:00:00

  • Selectin GMP-140 (CD62; PADGEM) binds to sialosyl-Le(a) and sialosyl-Le(x), and sulfated glycans modulate this binding.

    abstract：:GMP-140 (CD62; PADGEM) is a member of the selectin family expressed highly at the surface of platelets and endothelial cells by agonists such as thrombin or phorbol esters. Previous studies indicate that the lectin domain of GMP-140 recognizes sialosyl-Le(x) (SLex) and to a lesser extent Le(x) (Polley MJ, et al., Proc...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)92069-v

    authors： Handa K,Nudelman ED,Stroud MR,Shiozawa T,Hakomori S

    更新日期：1991-12-31 00:00:00

  • Using a ubiquitin ligase as an unfolded protein sensor.

    abstract：:A significant fraction of all proteins are misfolded and must be degraded. The ubiquitin-proteasome pathway provides an essential protein quality control function necessary for normal cellular homeostasis. Substrate specificity is mediated by proteins called ubiquitin ligases. In the endoplasmic reticulum (ER) a speci...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.12.109

    authors： Mallinger A,Wen HM,Dankle GM,Glenn KA

    更新日期：2012-02-03 00:00:00

  • Parathyroid hormone-related protein is produced by cultured endothelial cells: a possible role in angiogenesis.

    abstract：:Parathyroid hormone-related protein (PTHrP) is produced by various normal and neoplastic tissues. Even if the physiological function(s) of PTHrP is unclear, evidence suggests that the protein may participate in the local regulation of smooth muscle contractility. We show here that PTHrP is produced in endothelial cell...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.1107

    authors： Rian E,Jemtland R,Olstad OK,Endresen MJ,Grasser WA,Thiede MA,Henriksen T,Bucht E,Gautvik KM

    更新日期：1994-01-28 00:00:00

  • Pancreatic cancer stimulates pancreatic stellate cell proliferation and TIMP-1 production through the MAP kinase pathway.

    abstract：:Pancreatic adenocarcinoma is characterized by an intense desmoplastic reaction that surrounds the tumor. Pancreatic stellate cells (PSCs) are thought to be responsible for production of this extracellular matrix. When activated, PSCs have a myofibroblast phenotype and produce not only components of the extracellular m...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.08.229

    authors： Yoshida S,Yokota T,Ujiki M,Ding XZ,Pelham C,Adrian TE,Talamonti MS,Bell RH Jr,Denham W

    更新日期：2004-10-29 00:00:00

  • Hypertonicity increases sodium transporters in cortical collecting duct cells independently of PGE2.

    abstract：:Cyclooxygenase-2 (COX-2) expression is increased by hypertonicity. Therefore we hypothesized that hypertonicity increased PGE(2) can modulate the sodium transporters (Na(+)/K(+)-ATPase: NKA, epithelial sodium channel: ENaC, and sodium hydrogen exchanger: NHE) in M1 cortical collecting duct (CCD) cells. We demonstrated...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.01.028

    authors： Nasrallah R,Paris G,Hébert RL

    更新日期：2012-02-10 00:00:00

  • Hepatocyte growth factor induces mitogenic reaction to the rabbit gastric epithelial cells in primary culture.

    abstract：:Hepatocyte Growth Factor (HGF) was originally identified as hepatotrophic factor for liver regeneration. More recently, HGF was revealed to stimulate proliferation of various epithelial cells. At present study, we have investigated the mitogenic effect of HGF on gastric epithelial cells in the primary culture system. ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1250

    authors： Takahashi M,Ota S,Terano A,Yoshiura K,Matsumura M,Niwa Y,Kawabe T,Nakamura T,Omata M

    更新日期：1993-03-15 00:00:00

  • Hepatic glucose-6-phosphatase-α deficiency leads to metabolic reprogramming in glycogen storage disease type Ia.

    abstract：:Glycogen storage disease type Ia (GSD-Ia) is caused by a deficiency in glucose-6-phosphatase-α (G6Pase-α or G6PC), a key enzyme in endogenous glucose production. This autosomal recessive disorder is characterized by impaired glucose homeostasis and long-term complications of hepatocellular adenoma/carcinoma (HCA/HCC)....

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.03.083

    authors： Cho JH,Kim GY,Mansfield BC,Chou JY

    更新日期：2018-04-15 00:00:00

  • Novel agmatine analogue, gamma-guanidinooxypropylamine (GAPA) efficiently inhibits proliferation of Leishmania donovani by depletion of intracellular polyamine levels.

    abstract：:The efficacy of gamma-guanidinooxypropylamine (GAPA), a novel agmatine analogue against protozoan parasite, Leishmaniadonovani was evaluated. Wild-type and ornithine decarboxylase-overexpressors of L. donovani were used to study the effect and mode of action of this inhibitor. GAPA inhibited the growth of both promast...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.07.143

    authors： Singh S,Jhingran A,Sharma A,Simonian AR,Soininen P,Vepsalainen J,Khomutov AR,Madhubala R

    更新日期：2008-10-10 00:00:00

  • Inactivation of 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine by a plasma acetylhydrolase: higher activities in hypertensive rats.

    abstract：:We have partially characterized the properties of a specific acetylhydrolase in plasma from spontaneous hypertensive rats. This enzyme inactivates 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine (a lipid involved in platelet aggregating, hypotensive, and allergic responses) by removal of the acetate group. The extent of ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)91778-3

    authors： Blank ML,Hall MN,Cress EA,Snyder F

    更新日期：1983-06-15 00:00:00