Mutual suppression of miR-125a and Lin28b in human hepatocellular carcinoma cells.

Abstract:

:MicroRNA-125a exhibits an antiproliferative activity and is downregulated in several types of tumors, including hepatocellular carcinoma where it targets sirtuin-7, matrix metalloproteinase-11, and c-Raf. Another target of miR-125a is Lin28, a pluripotency factor that is generally undetectable in differentiated cells but is often upregulated/reactivated in tumors where it acts as an oncogenic factor promoting cell proliferation and tumor progression. In this study we show that downregulation of Lin28b by miR-125a partially accounts for its antiproliferative activity toward hepatocellular carcinoma cells. We also found that Lin28b is able to bind a conserved GGAG motif of pre-miR-125a and to inhibit its maturation in hepatocellular carcinoma cells. Reciprocal inhibition between miR-125a and Lin28b reasonably generates a positive feedback loop where reactivation of Lin-28b inhibits the expression of both miR-125a and let-7, reinforcing its own expression and leading to a marked overexpression of the mitogenic targets of the two miRNAs. On the other hand, perturbation of these circuits by overexpression of miR-125a suppresses Lin28b leading to a decreased cell proliferation. Overall, these data support a tumor suppressive role for miR-125a and contribute to the elucidation of its molecular targets.

authors

Panella M,Mosca N,Di Palo A,Potenza N,Russo A

doi

10.1016/j.bbrc.2018.04.167

subject

Has Abstract

pub_date

2018-06-07 00:00:00

pages

824-827

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(18)30954-9

journal_volume

500

pub_type

杂志文章
  • Resynthesis of insulin from its A and B chains in the presence of denaturants.

    abstract::Reoxidation of the reduced insulin A and B chains in 8 M urea leads to a recovery of native insulin of 2-7% whereas in 6 M guanidine or 0.5 mM dodecylsulfate very little, if any, resynthesis of insulin could be detected. Considering all the possibilities of different oligomeric forms containing one or both of the chai...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(87)90548-1

    authors: Qian YQ,Tsou CL

    更新日期:1987-07-31 00:00:00

  • NLRC4 inflammasome activation regulated by TNF-α promotes inflammatory responses in nonalcoholic fatty liver disease.

    abstract::The prevalence of nonalcoholic fatty liver disease (NAFLD) is high and increasing throughout the world. The intense sterile inflammation stemming from steatosis plays a significant role in the development of NAFLD, but the mechanism is unclear. We found that the NLRC4 inflammasome was activated in an in vitro cell mod...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2019.02.099

    authors: Chen Y,Ma K

    更新日期:2019-04-09 00:00:00

  • Depression of p53-independent Akt survival signals in human oral cancer cells bearing mutated p53 gene after exposure to high-LET radiation.

    abstract::Although mutations and deletions in the p53 tumor suppressor gene lead to resistance to low linear energy transfer (LET) radiation, high-LET radiation efficiently induces cell lethality and apoptosis regardless of the p53 gene status in cancer cells. Recently, it has been suggested that the induction of p53-independen...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2012.06.004

    authors: Nakagawa Y,Takahashi A,Kajihara A,Yamakawa N,Imai Y,Ota I,Okamoto N,Mori E,Noda T,Furusawa Y,Kirita T,Ohnishi T

    更新日期:2012-07-13 00:00:00

  • Expression of chicken liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase in Escherichia coli.

    abstract::Chicken liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase was expressed in E. coli by using a pET3a T7 RNA polymerase-based expression system and was purified to homogeneity. The kinase and bisphosphatase of the expressed bifunctional enzyme had kinetic properties identical to those of the native chicken live...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1995.1581

    authors: Li L,Yao WZ,Lange AJ,Pilkis SJ,Dong MQ,Yin Y,Xu GJ

    更新日期:1995-04-26 00:00:00

  • Interaction of DNA hairpin loops and a complementary strand by a triplet of base pairs.

    abstract::Hypothesis of non-enzymatic recognition of primordial tRNA and mRNA precursors is experimentally approached. DNA hairpins containing a different number of deoxyguanosine residues in the loop are analyzed for their binding ability to a chemically fixed single-strand of oligo(dC). In presence of small Mg2+ concentration...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(88)80971-9

    authors: Baumann U,Frank R,Blöcker H

    更新日期:1988-12-30 00:00:00

  • Phosphorylation of the carboxyl-terminal domain of the zeta 1 subunit is not responsible for potentiation by TPA of the NMDA receptor channel.

    abstract::The carboxyl-terminal domain of the zeta 1 subunit of the mouse NMDA receptor channel produced as a fusion protein with GST was phosphorylated in vitro by PKC. A mutant of the zeta 1 subunit without serine or threonine residues in the carboxyl-terminal domain (zeta 1-2-NST) was constructed and was expressed alone or t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1993.2426

    authors: Yamakura T,Mori H,Shimoji K,Mishina M

    更新日期:1993-11-15 00:00:00

  • Deubiquitinating enzymes: their diversity and emerging roles.

    abstract::A growing number of important regulatory proteins within cells are modified by conjugation of ubiquitin, a well-conserved 76-amino-acid polypeptide. The ubiquitinated proteins are targeted to proteasome for degradation or alternative metabolic fates, such as triggering of plasma membrane endocytosis and trafficking to...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章,评审

    doi:10.1006/bbrc.1999.1880

    authors: Chung CH,Baek SH

    更新日期:1999-12-29 00:00:00

  • Demonstration of an antigenic protein specific for Salmonella typhi.

    abstract::Current studies were undertaken to determine the presence of a specific antigenic protein on the outer membrane of Salmonella typhi. Immunoblot analysis using sera from patients with fevers revealed that the 50 kD band was specifically recognized only by typhoid sera. The 50 kD band located on the outer membrane is pr...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(05)81417-2

    authors: Ismail A,Hai OK,Kader ZA

    更新日期:1991-11-27 00:00:00

  • An integrated RNA-Seq and network study reveals a complex regulation process of rice embryo during seed germination.

    abstract::Seed germination is a crucial stage for plant development and agricultural production. To investigate its complex regulation process, the RNA-Seq study of rice embryo was conducted at three time points of 0, 12 and 48 h post imbibition (HPI). Dynamic transcriptional alterations were observed, especially in the early s...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2015.06.110

    authors: Wei T,He Z,Tan X,Liu X,Yuan X,Luo Y,Hu S

    更新日期:2015-08-14 00:00:00

  • Coordinate regulation/localization of the carbohydrate responsive binding protein (ChREBP) by two nuclear export signal sites: discovery of a new leucine-rich nuclear export signal site.

    abstract::Carbohydrate response element binding protein (ChREBP) is responsible for conversion of dietary carbohydrate to storage fat in liver by coordinating expression of the enzymes that channel glycolytic pyruvate into lipogenesis. The activation of ChREBP in response to high glucose is nuclear localization and transcriptio...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2009.11.115

    authors: Fukasawa M,Ge Q,Wynn RM,Ishii S,Uyeda K

    更新日期:2010-01-08 00:00:00

  • Osthole promotes the suppressive effects of cisplatin on NRF2 expression to prevent drug-resistant cervical cancer progression.

    abstract::Cervical cancer is one of the most commonly diagnosed lethal malignancies among gynecological malignant tumors worldwide. Chemo-resistance is one of the key causal factors in cervical cancer death. Osthole (OST), a natural compound, exhibits various pharmacological activities, including anti-tumor effects. However, it...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2019.04.021

    authors: Su J,Zhang F,Li X,Liu Z

    更新日期:2019-06-25 00:00:00

  • OK-1035, a selective inhibitor of DNA-dependent protein kinase.

    abstract::Screening for inhibitors of DNA-dependent protein kinase (DNA-PK) revealed 3-cyano-5-(4-pyridyl)-6-hydrazonomethyl-2-pyridone, designated OK-1035, to be a potent and selective inhibitor. When a synthetic peptide was used as a substrate, OK-1035 caused 50% inhibition of DNA-PK activity at 8 microM, a concentration more...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1995.2431

    authors: Take Y,Kumano M,Hamano Y,Fukatsu H,Teraoka H,Nishimura S,Okuyama A

    更新日期:1995-10-04 00:00:00

  • Cloning and expression of genes coding for carbohydrate degrading enzymes of Anaerocellum thermophilum in E.coli.

    abstract::A gene library of Anaerocellum thermophilum Z-1320 was constructed in Escherichia coli using plasmid pUC18. Clones were selected by screening for hydrolysis of CMC, MU-Cel, lichenan and xylan. Recombinant clones expressing two beta-1,4-glucanases and two different xylanases were obtained. Both beta-1,4-glucanases were...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1994.2038

    authors: Bolshakova EV,Ponomariev AA,Novikov AA,Svetlichnyi VA,Velikodvorskaya GA

    更新日期:1994-07-29 00:00:00

  • Molecular tryst peeping: detection of interactions between nonlabeled nucleic acids by fluorescence resonance energy transfer.

    abstract::We have developed a new method for monitoring the interactions between nonlabeled RNAs that involves detection of fluorescence resonance energy transfer (FRET) between two DNA probes with different fluorescent label. The sequences of the probes are complementary to those of the RNAs. In this study, we examined the int...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2001.6098

    authors: Ota N,Sato T,Taira K,Ohkawa J

    更新日期:2001-12-21 00:00:00

  • Sodium ion modulates collagen types in human fibroblastic cells in culture.

    abstract::Collagen synthesis in human fibroblasts was increased by the presence of 0.1M excess NaCl in the culture medium. Type analysis of the collagen produced by the cells showed the increase in the ratio of type III collagen to the total collagen. Similar NaCl effects were observed in several cell lines derived from differe...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(83)91577-2

    authors: Hata R,Sunada H,Nagai Y

    更新日期:1983-11-30 00:00:00

  • Inositol 1,4,5-trisphosphate-induced Ca2+ release from permeabilized mastocytoma cells.

    abstract::The effect of inositol 1,4,5-trisphosphate (IP3) on Ca2+ release in the transformed murine mast cells, mastocytoma P-815 cells permeabilized with digitonin was studied. Ca2+ was sequestered by intracellular organelles in the presence of ATP until the medium free Ca2+ concentration was lowered to a new steady-state lev...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(86)90940-x

    authors: Muto Y,Tohmatsu T,Yoshioka S,Nozawa Y

    更新日期:1986-02-26 00:00:00

  • Cepharanthine induces apoptosis through reactive oxygen species and mitochondrial dysfunction in human non-small-cell lung cancer cells.

    abstract::Cepharanthine is a medicinal plant-derived natural compound which possesses potent anti-cancer properties. However, there is little report about its effects on lung cancer cells. In this study, we investigated the effects of cepharanthine on the cell viability and apoptosis in human non-small-cell lung cancer H1299 an...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2015.02.131

    authors: Hua P,Sun M,Zhang G,Zhang Y,Tian X,Li X,Cui R,Zhang X

    更新日期:2015-05-01 00:00:00

  • Knockdown of transcription factor forkhead box O3 (FOXO3) suppresses erythroid differentiation in human cells and zebrafish.

    abstract::Our previous study on the dynamic transcriptomes activated during human erythropoiesis suggested that transcription factor forkhead box O3 (FOXO3) possibly plays a role in erythroid differentiation. Functional studies in human cell line TF-1 indicated that FOXO3 knockdown repressed erythropoietin (EPO)-induced erythro...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2015.03.128

    authors: Wang H,Li Y,Wang S,Zhang Q,Zheng J,Yang Y,Qi H,Qu H,Zhang Z,Liu F,Fang X

    更新日期:2015-05-15 00:00:00

  • The functional unit of Na,K-ATPase is a monomeric alphabeta protomer.

    abstract::The ouabain-resistant and ouabain-sensitive alpha-subunit cRNAs in various molar ratios were injected into Xenopus oocytes together with cRNA for the beta-subunit. The ouabain-resistant ATPase activity, as well as ouabain-resistant Rb+ uptake, of the injected oocytes increased linearly with increasing the amount of cR...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2001.4292

    authors: Takeda K,Kawamura M

    更新日期:2001-02-09 00:00:00

  • Newly expressed proteins of mouse embryonic fibroblasts irradiated to be inactive.

    abstract::It has been found that post-radiation mouse embryonic fibroblasts can well maintain the pluripotency in human embryonic stem cells. However, the molecular mechanism remains unclear. In the present study, the new protein expression profile of post-radiation mouse embryonic fibroblasts was analyzed by immobilized pH gra...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2004.01.089

    authors: Xie CQ,Lin G,Luo KL,Luo SW,Lu GX

    更新日期:2004-03-12 00:00:00

  • Premutation huntingtin allele adopts a non-B conformation and contains a hot spot for DNA damage.

    abstract::The expansion of a CAG trinucleotide repeat (TNR) sequence has been linked to several neurological disorders, for example, Huntington's disease (HD). In HD, healthy individuals have 5-35 CAG repeats. Those with 36-39 repeats have the premutation allele, which is known to be prone to expansion. In the disease state, gr...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2011.11.013

    authors: Jarem DA,Delaney S

    更新日期:2011-12-09 00:00:00

  • p56lck phosphorylation by Ca2+/calmodulin-dependent protein kinase type II.

    abstract::Ca2+/calmodulin-dependent protein kinases are implicated in regulating the Ca2+ signaling involved in T cell activation and in thymocyte selection. One of the earliest events in signaling through the T cell antigen receptor is activation of the protein tyrosine kinase p56lck. Following T cell activation or signaling t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1994.1010

    authors: Bland MM,McDonald OB,Carrera AC

    更新日期:1994-01-14 00:00:00

  • Arctigenin reduces blood pressure by modulation of nitric oxide synthase and NADPH oxidase expression in spontaneously hypertensive rats.

    abstract::Arctigenin is a bioactive constituent from dried seeds of Arctium lappa L., which was traditionally used as medicine. Arctigenin exhibits various bioactivities, but its effects on blood pressure regulation are still not widely studied. In this study, we investigated antihypertensive effects of arctigenin by long-term ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2015.11.041

    authors: Liu Y,Wang G,Yang M,Chen H,zhao Y,Yang S,Sun C

    更新日期:2015-12-25 00:00:00

  • Clathrin-coated vesicles from human placenta contain GTP-binding proteins.

    abstract::Biochemical and morphological techniques were used to investigate the GTP-binding proteins of clathrin-coated vesicles. Binding of [3H]GTP to clathrin coats was demonstrated by electron microscopic autoradiography. Purified coated vesicles bound 5.2 pmol [35S]GTP gamma S/mg protein. Addition of GTP or GTP gamma S, but...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(91)90505-2

    authors: Lenhard JM,Levy MA,Stahl PD

    更新日期:1991-01-15 00:00:00

  • A putative sirolimus (rapamycin) effector protein.

    abstract::Sirolimus (rapamycin), a new immunosuppressive drug, inhibits proliferation of a wide spectrum of T and B cells. The immunosuppressive mechanism of sirolimus is still unclear. We recently isolated a membrane associated protein with an apparent molecular weight of 210 kDa, p210, from cultured Molt 4 cells and BJAB cell...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1994.2140

    authors: Chen Y,Chen H,Rhoad AE,Warner L,Caggiano TJ,Failli A,Zhang H,Hsiao CL,Nakanishi K,Molnar-Kimber KL

    更新日期:1994-08-30 00:00:00

  • High chondroitin sulfate proteoglycan 4 expression correlates with poor outcome in patients with breast cancer.

    abstract::Chondroitin sulfate proteoglycan 4 (CSPG4), a transmembrane proteoglycan originally identified in melanoma cells, has been reported to be expressed in breast cancer cells. This study was performed to examine the expression and significance of CSPG4 in a cohort of breast cancer patients. Immunohistochemical analysis of...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2013.10.093

    authors: Hsu NC,Nien PY,Yokoyama KK,Chu PY,Hou MF

    更新日期:2013-11-15 00:00:00

  • Overexpression of miR-183/-96/-182 triggers neuronal cell fate in Human Retinal Pigment Epithelial (hRPE) cells in culture.

    abstract::miR-183 cluster, composed of miR-183/-96/-182 genes, is highly expressed in the adult retina, particularly in photoreceptors. It involves in development, maturation and normal function of neuroretina. Ectopic overexpression of miR-183/-96/-182 genes was performed to assess reprogramming of hRPE cells. They were amplif...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2016.12.071

    authors: Davari M,Soheili ZS,Samiei S,Sharifi Z,Pirmardan ER

    更新日期:2017-01-29 00:00:00

  • Functional reconstitution of monomeric CYP3A4 with multiple cytochrome P450 reductase molecules in Nanodiscs.

    abstract::Traditional reconstitution of membrane cytochromes P450 monooxygenase system requires efficient solubilization of both P450 heme enzymes and redox partner NADPH dependent reductase, CPR, either in mixed micellar solution or by incorporation in liposomes. Here we describe a simple alternative approach to assembly of so...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2010.06.058

    authors: Grinkova YV,Denisov IG,Sligar SG

    更新日期:2010-07-23 00:00:00

  • NLRP3/ASC/Caspase-1 axis and serine protease activity are involved in neutrophil IL-1β processing during Streptococcus pneumoniae infection.

    abstract::Streptococcus pneumoniae is a pathogenic bacterium that can cause severe invasive diseases, such as pneumonia, otitis media and meningitis. The pro-inflammatory cytokine, IL-1β, has been reported to play important role in host defense against S. pneumoniae. The mechanism of IL-1β maturation and secretion in macrophage...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2019.04.004

    authors: Zhang T,Du H,Feng S,Wu R,Chen T,Jiang J,Peng Y,Ye C,Fang R

    更新日期:2019-06-04 00:00:00

  • Regulation of oscillations in filamentous actin content in polymorphonuclear leukocytes stimulated with leukotriene B(4) and platelet-activating factor.

    abstract::Stimulation of neutrophils with LTB(4) or PAF results in the production of a rapidly oscillating actin polymerization/depolymerization response. Treatment of neutrophils with inhibitors of PKC prior to stimulation with ligand resulted in a masking of the F-actin oscillations. Because myosin has been shown to be a subs...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1999.1222

    authors: Rengan R,Omann GM

    更新日期:1999-08-27 00:00:00