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Experimental considerations on the measurement of prostaglandins during long-time incubations of neonatal mouse calvaria.

Abstract：

:The influence of experimental conditions during long-time (72 h) incubations of neonatal mouse calvaria on the measurement of prostaglandins was investigated. Incubations of the cultured calvaria were carried out in the presence and absence of stimulating agents of bone resorption, such as thrombin and parathyroid hormone. It was found that during the first 24 h prostaglandin levels, estimated by gas chromatography negative ion chemical ionization mass spectrometry, did not correlate with calcium liberation, but were merely an artefact resulting from surgery by preparing the calvaria.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Leis HJ,Malle E,Hoffmann O,Klaushofer K,Fratzl-Zelman N,Koller K,Peterlik M

doi

10.1016/0006-291x(90)90365-t

subject

Has Abstract

pub_date

1990-06-15 00:00:00

pages

545-50

issue

2

eissn

0006-291X

issn

1090-2104

pii

0006-291X(90)90365-T

journal_volume

169

pub_type

杂志文章

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