Abstract:
:In this study, the full-length cDNA of the UDP-glucose pyrophosphorylase gene was isolated from jute by homologous cloning (primers were designed according to the sequence of UGPase gene of other plants) and modified RACE techniques; the cloned gene was designated CcUGPase. Using bioinformatic analysis, the gene was identified as a member of the UGPase gene family. Real-time PCR analysis revealed differential spatial and temporal expression of the CcUGPase gene, with the highest expression levels at 40 and 120d. PCR and Southern hybridization results indicate that the gene was integrated into the jute genome. Overexpression of CcUGPase gene in jute revealed increased height and cellulose content compared with control lines, although the lignin content remained unchanged. The results indicate that the jute UGPase gene participates in cellulose biosynthesis. These data provide an important basis for the application of the CcUGPase gene in the improvement of jute fiber quality.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Zhang G,Qi J,Xu J,Niu X,Zhang Y,Tao A,Zhang L,Fang P,Lin Ldoi
10.1016/j.bbrc.2013.11.053subject
Has Abstractpub_date
2013-12-13 00:00:00pages
153-8issue
3-4eissn
0006-291Xissn
1090-2104pii
S0006-291X(13)01946-3journal_volume
442pub_type
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