Isolation of two biologically active peptides, erythrotropin I and erythrotropin II from fetal calf intestine.

abstract：:2-Amino-3,8-dimethylimidazo(4,5-f)quinoxaline (MeIQx) is a potent mutagen found in cooked food. MeIQx and its isotopically labelled (13C, 15N2 and 14C) analogues were synthesised and used for metabolic studies in vivo. An equimolar mixture of MeIQx and its 13C, 15N2 stable isotope labelled analogue (containing tracer ...

journal_title：Biochemical and biophysical research communications

authors： Gooderham NJ,Watson D,Rice JC,Murray S,Taylor GW,Davies DS

更新日期：1987-11-13 00:00:00

abstract：:Major histocompatibility complex (MHC)-loading enhancers (MLE) have recently attracted attention because of their ability to enhance the efficacy of peptide immunotherapeutics. As small molecular weight compounds, they influence the loading of peptides in MHC molecules by converting them from a non-receptive to a rece...

journal_title：Biochemical and biophysical research communications

authors： Afridi S,Shaheen F,Roetzschke O,Shah ZA,Abbas SC,Siraj R,Makhmoor T

更新日期：2015-01-16 00:00:00

abstract：:This study evaluates the influence of S1033 vinculin phosphorylation on the mechanical properties of cells. We demonstrate that MEFvcl KO cells transfected with the non-phosphorylatable eGFP-vinculin mutant S1033A are of lower stiffness compared to MEFvcl Rescue and phospho-mimicking mutant S1033D cells, which were of...

journal_title：Biochemical and biophysical research communications

authors： Auernheimer V,Goldmann WH

更新日期：2014-07-25 00:00:00

abstract：:Metformin, a widely used anti-diabetic drug, is emerging as a potential anticancer agent but the mechanisms involved remain incompletely understood. Here, we demonstrate that the potency of metformin induced AMPK activation, as shown by the phosphorylation of its substrates acetyl-CoA carboxylase (ACC) at Ser(79) and ...

journal_title：Biochemical and biophysical research communications

authors： Sinnett-Smith J,Kisfalvi K,Kui R,Rozengurt E

更新日期：2013-01-04 00:00:00

abstract：:A rapid and easy method for the production of both the 4R and 4S tritium labeled isomers of either NADH or NADPH has been developed. The method requires the use of only a single labeled compound (D-[1(-3)H] glucose), and two enzymes (glucose dehydrogenase from Bacillus sp. and alcohol dehydrogenase from Thermoanaerobi...

journal_title：Biochemical and biophysical research communications

authors： Valera V,Fung M,Wessler AN,Richards WR

更新日期：1987-10-14 00:00:00

abstract：:We have isolated calpromotin, a protein reported abundant in human red cells and shown to be of significance for KCl transport. We show that calpromotin is identical to a radical scavenger protein, thiol-specific antioxidant protein (TSA). Calpromotin is known to exist partially as a large complex of identical subunit...

journal_title：Biochemical and biophysical research communications

authors： Kristensen P,Rasmussen DE,Kristensen BI

更新日期：1999-08-19 00:00:00

abstract：:Neurotrophins are essential for the development and survival of catecholaminergic neurons. However, the critical pathway for expression of the tyrosine hydroxylase (TH) gene induced by neurotrophin is still unclear. Here we found that Ras/MEK pathway is required for NGF-induced expression of the TH gene in PC12D cells...

journal_title：Biochemical and biophysical research communications

authors： Suzuki T,Kurahashi H,Ichinose H

更新日期：2004-03-05 00:00:00

abstract：:Histone deacetylases (HDACs) hyper-activity in hepatocellular carcinoma (HCC) is often associated with patients' poor prognosis. Our previous study has shown that resminostat, a novel HDAC inhibitor (HDACi), activated mitochondrial permeability transition pore (mPTP)-dependent apoptosis pathway in HCC cells. Here we e...

journal_title：Biochemical and biophysical research communications

authors： Peng X,Zhang D,Li Z,Fu M,Liu H

更新日期：2016-09-02 00:00:00

abstract：:Binding of [3H]-staurosporine to different protein kinases was time-dependent, reversible and saturable. Scatchard analysis of saturation isotherms indicated one class of binding sites for [3H]-staurosporine with dissociation constants (KD) of 9.6, 2.0, 3.0 and 7.4 nM for protein kinase C, cAMP-dependent protein kinas...

journal_title：Biochemical and biophysical research communications

authors： Herbert JM,Seban E,Maffrand JP

更新日期：1990-08-31 00:00:00

abstract：:Long noncoding RNAs (lncRNAs) are acknowledged as crucial regulators involved in multiple pathological processes, including cancer. Although some lncRNAs are studied in melanoma, the association between lncRNA and melanoma progression still remains vague. And the function of LINC00963 in melanoma is waiting for invest...

journal_title：Biochemical and biophysical research communications

authors： Jiao H,Jiang S,Wang H,Li Y,Zhang W

更新日期：2018-09-26 00:00:00

abstract：:PRPK (p53-related protein kinase) has been reported as a novel protein kinase which binds to the tumor suppressor protein p53 and induces phosphorylation of p53 at Ser 15. To identify novel binding partners of PRPK, we performed a yeast two-hybrid screening and isolated an expressed sequence tag CGI-121 by which a 20-...

journal_title：Biochemical and biophysical research communications

authors： Miyoshi A,Kito K,Aramoto T,Abe Y,Kobayashi N,Ueda N

更新日期：2003-04-04 00:00:00

abstract：:Biochemical properties of bovine lactoperoxidase isolated from milk and recombinant bovine lactoperoxidase expressed by Chinese hamster ovary cells were compared. The natural and recombinant lactoperoxidases showed the same conformational features as determined by circular dichroism (CD) measurements. The alpha-helix,...

journal_title：Biochemical and biophysical research communications

authors： Watanabe S,Murata S,Kumura H,Nakamura S,Bollen A,Moguilevsky N,Shimazaki K

更新日期：2000-08-11 00:00:00

abstract：:The role of microRNAs (miRNAs) in how microbiota influence the host intestinal immune system is not fully understood. We compared the expression profiles of miRNAs and mRNAs in lamina propria leukocytes (LPL) in the large intestines of germ-free (GF) and specific pathogen-free (SPF) mice. Microarray analysis revealed ...

journal_title：Biochemical and biophysical research communications

authors： Ohsaka F,Karatsu Y,Kadota Y,Tochio T,Takemura N,Sonoyama K

更新日期：2021-01-01 00:00:00

abstract：:Characterization of functional vessels is required either for monitoring hemodynamics or patterning of functional vasculature in experimental models. Haemoglobin (Hb) staining is a traditionally used approach for determining the differentiation of erythroid cells. In this investigation, we tested if HB staining can be...

journal_title：Biochemical and biophysical research communications

authors： Zheng PP,Severijnen LA,Willemsen R,Kros JM

更新日期：2009-03-20 00:00:00

abstract：:Low molecular weight-PTP has been reported as a redox-sensitive protein during both platelet-derived growth factor and integrin signalling. In response to oxidation the phosphatase undergoes a reversible inactivation, which in turn leads to the increase in tyrosine phosphorylation of its substrates and the properly ex...

journal_title：Biochemical and biophysical research communications

authors： Giannoni E,Raugei G,Chiarugi P,Ramponi G

更新日期：2006-09-22 00:00:00

abstract：:Intramolecularly quenched fluorogenic peptide substrates with the general sequence: DABCYL-Lys-Phe-Gly-Gly-Xxx-Ala-EDANS have been utilized to explore the effect of the hydrophobicity of amino acid side chains in the P2' position on the steady-state kinetic constants for papain catalyzed hydrolysis. The results demons...

journal_title：Biochemical and biophysical research communications

authors： García-Echeverría C,Rich DH

更新日期：1992-09-16 00:00:00

abstract：:Ling Zhi-8 (LZ-8), a novel immunomodulatory protein, markedly enhanced the expression of CD11b, but not CD11a, CD13, CD14, CD18, CD33 or HLA-DR, on the U937 cell line in a dose-dependent fashion. It also induced ICAM-1 expression on vascular endothelial cells and significantly augmented gamma - interferon-induced cell...

journal_title：Biochemical and biophysical research communications

authors： Miyasaka N,Inoue H,Totsuka T,Koike R,Kino K,Tsunoo H

更新日期：1992-07-15 00:00:00

abstract：:We have previously reported the establishment of preadipocyte cell lines, termed dedifferentiated fat (DFAT) cells, from mature adipocytes of various animals. DFAT cells possess long-term viability and can redifferentiate into adipocytes both in vivo and in vitro. Furthermore, DFAT cells can transdifferentiate into os...

journal_title：Biochemical and biophysical research communications

authors： Kazama T,Fujie M,Endo T,Kano K

更新日期：2008-12-19 00:00:00

abstract：:We have reexamined the intracellular localization of the ubiquitously expressed target membrane SNAP receptor (t-SNARE), SNAP-23. While SNAP-23 appears on the plasma membrane, in the cell types examined there is a significant pool associated with endosomal compartments. Immuno-staining and expression of green fluoresc...

journal_title：Biochemical and biophysical research communications

authors： Chen D,Whiteheart SW

更新日期：1999-02-16 00:00:00

abstract：:The anthrax lethal factor (LF) is a Zn(2+)-endopeptidase specific for mitogen-activated protein kinase kinases (MAPKKs), which are cleaved within their N-terminal region. Much line of effort was carried out to elucidate the catalytic activity of LF for designing the inhibitor and to understand the cellular mechanism o...

journal_title：Biochemical and biophysical research communications

authors： Kim J,Chai YG,Yoon MY

更新日期：2004-01-02 00:00:00

abstract：:The changes of local field potentials (LFP, mainly gamma rhythm and theta rhythm) in the brain are closely related to learning and memory formation. Reduced gamma rhythm (20-50 Hz) and theta rhythm (4-10 Hz) has been observed in the progression of Alzheimer's disease (AD), but it is not clear whether it is related to ...

journal_title：Biochemical and biophysical research communications

authors： Zhang Z,Jing Y,Ma Y,Duan D,Li B,Hölscher C,Li C,Wei J,Gao A,Shang L,Tao F,Xing Y

更新日期：2020-05-14 00:00:00

abstract：:Using atomic force microscopy (AFM), we find that RecA-single-stranded DNA (RecA-ssDNA) filaments, in the presence of single-stranded DNA-binding (SSB) protein, organize into left-handed bundles, which differ from the previously reported disordered aggregates formed when SSB is excluded from the reaction. In addition,...

journal_title：Biochemical and biophysical research communications

authors： Shi WX,Larson RG

更新日期：2007-06-08 00:00:00

abstract：:In rat mesenteric vessels changes in external pH (pHo) alter tension. Changes in intracellular pH (pHi) and calcium ([Ca2+]i) have both been suggested to underlie the tension changes. As no simultaneous measurements of pHi and [Ca2+]i had been made, however, the relative importance and temporal relationship between pH...

journal_title：Biochemical and biophysical research communications

authors： Austin C,Dilly K,Eisner D,Wray S

更新日期：1996-05-15 00:00:00

abstract：:Retinoic acid (RA) is described as an inhibitor of prostate cancer cell growth. We utilized reverse transcription-polymerase chain reaction (RT-PCR) to analyze expression of different isoforms of fibroblast growth factor 8 (FGF8) in response to RA. Results in the prostate cancer cell line LNCaP show that whereas overa...

journal_title：Biochemical and biophysical research communications

authors： Brondani V,Hamy F

更新日期：2000-05-27 00:00:00

abstract：:Ascorbic acid is well known to acutely stimulate norepinephrine synthesis in neurosecretory cells, but it has also been shown over several days to increase tyrosine hydroxylase mRNA and norepinephrine synthesis in cultured neurons. Since tyrosine hydroxylase is the rate-limiting step in catecholamine synthesis, an eff...

journal_title：Biochemical and biophysical research communications

authors： May JM,Qu ZC,Meredith ME

更新日期：2012-09-14 00:00:00

abstract：:Human platelets are less responsive to PAF at 37 degrees than at 25 degrees. They can be desensitized to the effects of PAF by pre-exposure to small concentrations. In both cases desensitization appears to be accompanied by a decreased affinity of the high affinity site for PAF rather than loss of binding sites. Alter...

journal_title：Biochemical and biophysical research communications

authors： Chesney CM,Pifer DD,Huch KM

更新日期：1985-02-28 00:00:00

abstract：:Mouse embryos at implantation stage were stained successfully with lacto-N-fucopentaose I (LNF-I) bearing neoglycoprotein labeled with rhodamine synthesized by us for the first time. The fluorescent neoglycoproteins carrying LNF-II, -III, LND-I, or LNT failed to stain the embryos. The embryo was stained only at the ce...

journal_title：Biochemical and biophysical research communications

authors： Yamagata T,Yamazaki K

更新日期：1991-12-31 00:00:00

abstract：:Laminin components were solubilized from basement membranes of amnion, chorion and chorionic microvessels of human placenta without prior protease digestion. These structures, after isolation, were initially processed in a sonicator bath containing a solution of Triton X-100, EDTA and 2M NaCl and the laminins extracte...

journal_title：Biochemical and biophysical research communications

authors： Ohno M,Martinez-Hernandez A,Ohno N,Kefalides NA

更新日期：1983-05-16 00:00:00

abstract：:Lipoprotein lipase (LPL) catalyzes the hydrolysis of the triacylglycerol component of triacylglycerol-rich lipoproteins. There are 4 cysteine pairs that are completely conserved among LPLs of all species known. We examined the functional importance of each of the cysteine pairs in enzyme catalysis by examining LPLs pr...

journal_title：Biochemical and biophysical research communications

authors： Lo JY,Smith LC,Chan L

更新日期：1995-01-05 00:00:00

abstract：:Chromatin-associated high mobility group (HMG) proteins of the HMGB family are versatile architectural factors assisting various DNA-dependent processes such as transcription and recombination. Here, transgenic tobacco lines were generated that ectopically express the maize HMGB1 protein, as detected by immunoblot ana...

journal_title：Biochemical and biophysical research communications

authors： Lichota J,Ritt C,Grasser KD

更新日期：2004-05-21 00:00:00