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Synthesis of 4R- and 4S-tritium labeled NADPH for the determination of the coenzyme stereospecificity of NADPH: protochlorophyllide oxidoreductase.

Abstract：

:A rapid and easy method for the production of both the 4R and 4S tritium labeled isomers of either NADH or NADPH has been developed. The method requires the use of only a single labeled compound (D-[1(-3)H] glucose), and two enzymes (glucose dehydrogenase from Bacillus sp. and alcohol dehydrogenase from Thermoanaerobium brockii) which are specific for the pro S and pro R hydrogens, respectively, of either NADH or NADPH. The 4R and 4S tritium labeled isomers of NADPH have been used to determine that NADPH:protochlorophyllide oxidoreductase from etiolated wheat was specific for the pro S hydrogen of NADPH.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Valera V,Fung M,Wessler AN,Richards WR

doi

10.1016/0006-291x(87)91141-7

subject

Has Abstract

pub_date

1987-10-14 00:00:00

pages

515-20

issue

1

eissn

0006-291X

issn

1090-2104

pii

0006-291X(87)91141-7

journal_volume

148

pub_type

杂志文章

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