当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Expression of rat interleukin-5 and generation of neutralizing antiserum: a comparative study of rat IL-5 produced in Escherichia coli and insect cells.

Expression of rat interleukin-5 and generation of neutralizing antiserum: a comparative study of rat IL-5 produced in Escherichia coli and insect cells.

Abstract：

:A cDNA coding for rat IL-5 was obtained by RT-PCR from total spleen RNA. With the exception of a single a.a. replacement at position 85 (L-P), it is identical to the published sequence obtained by retroviral gene transfer. This cDNA was used to express biologically active recombinant IL-5 in E. coli and in insect cells using a baculovirus system. Rat IL-5 is more active on B13, an IL-5 dependent cell line, when produced in insect cells (specific activity 1.47 x 10(11)UI/mg compared to 4.28 x 10(6)UI/mg). This increased activity seems to be associated with the presence of IL-5 homodimers in recombinant protein preparations. A rabbit antiserum raised against recombinant bacterial IL-5 specifically inhibited B13 proliferation induced by bacterial and baculoviral IL-5. The availability of such reagents should facilitate studying the role of IL-5 in different infectious diseases, experimental allergic encephalomyelitis and in transplantation biology where the rat represents a more suitable model than mice.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Pierrot C,Cocude C,Cêtre C,Godin C,Lafitte S,Capron M,Khalife J

doi

10.1006/bbrc.1998.9843

subject

Has Abstract

pub_date

1998-12-30 00:00:00

pages

756-60

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(98)99843-6

journal_volume

253

pub_type

杂志文章

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