Abstract:
:The promoter of rat multidrug resistance protein 3 (Mrp3) has been cloned and analyzed in the rat intestinal cell line (IEC-18 cells). A series of 5' deletion mutants of the Mrp3 promoter region were constructed and placed into the pGL3-Basic vector (luciferase reporter gene). Deletion analysis of the Mrp3 promoter identified a basal transcription element at -123/-106, two negative response regions at -2723/-1128 and -530/-443, respectively, and two positive response regions at -1063/-943 and -302/-157. Further site-directed mutagenesis analysis and gel mobility shift assays provided evidence for Sp1 and Sp3 binding within -123/-105 regions. These studies indicate that Sp1 and Sp3 may be involved in the regulation of the rat Mrp3 gene.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Tzeng SJ,Huang JDdoi
10.1006/bbrc.2002.6442subject
Has Abstractpub_date
2002-02-22 00:00:00pages
270-7issue
2eissn
0006-291Xissn
1090-2104pii
S0006291X02964429journal_volume
291pub_type
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
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更新日期:1987-09-30 00:00:00