Detection of purine cytosine permease of S. cerevisiae: use of antibodies against a synthetic peptide corresponding to a predicted sequence in the N-terminal domain of the protein.

abstract：:The (45)Ca(2+) influx into right-side-out resealed ghosts (RG) prepared from human red blood cells (RBC) was measured. The (45)Ca(2+) equilibration occurred with t(1/2)=2.5 min and the steady-state was reached after 17 min with the level of 22+/-2 micromol/L(packed cells) at 37 degrees C. The rate of the influx was 97...

journal_title：Biochemical and biophysical research communications

authors： Hudec R,Lakatos B,Orlický J,Varecka L

更新日期：2004-12-24 00:00:00

abstract：:Hepatitis C virus (HCV) is dependent on NS2-3 autocleavage and NS5A phosphorylation for its life cycle. We demonstrate that NS5A, when released from the NS2-5 polyprotein of the BK virus strain, is phosphorylated as two distinct forms, pp56 and pp58. Deletion analysis indicates that the appearance of pp58 requires NS2...

journal_title：Biochemical and biophysical research communications

authors： Liu Q,Bhat RA,Prince AM,Zhang P

更新日期：1999-01-27 00:00:00

abstract：:We have cloned and sequenced rat testis cDNAs coding for a calcium binding polypeptide similar to calcineurin beta subunit, the Ca(2+)-binding subunit of the Ca2+/calmodulin stimulated protein phosphatase. Rat testis cDNA library was screened with a monoclonal antibody Va1 raised against bovine brain calcineurin beta ...

journal_title：Biochemical and biophysical research communications

authors： Sugimoto M,Matsui H,Etoh S,Shimizu T,Nishio H,Moia LJ,Tokuda M,Itano T,Takenaka I,Hatase O

更新日期：1991-11-14 00:00:00

abstract：:To identify the target autoantigens in Vogt-Koyanagi-Harada disease, we made use of an immunoscreening of a bovine uveal cDNA expression library with serum samples obtained from patients with Vogt-Koyanagi-Harada disease. We identified a novel bovine antigen and homologous human autoantigen and designated it as UACA (...

journal_title：Biochemical and biophysical research communications

authors： Yamada K,Senju S,Nakatsura T,Murata Y,Ishihara M,Nakamura S,Ohno S,Negi A,Nishimura Y

更新日期：2001-02-02 00:00:00

abstract：:Effective treatment of neuropathic pain is challenging as its underlying mechanism remains largely unknown. Recently, the participation of brain-derived neurotrophic factor (BDNF) in neuropathic pain has been attracting increased attention. BDNF binds to a member of the tyrosine kinase receptor family, the TrkB recept...

journal_title：Biochemical and biophysical research communications

authors： Ikeda K,Hazama K,Itano Y,Ouchida M,Nakatsuka H

更新日期：2020-10-20 00:00:00

abstract：:We have previously demonstrated that the cyclin-dependent kinase inhibitor (Cki) Sic1 of Saccharomyces cerevisiae is phosphorylated in vitro by the CK2 kinase on Ser(201) residue. Moreover, we have collected evidence showing that Sic1 is functionally and structurally related to mammalian Cki p27(Kip1) and binds to the...

journal_title：Biochemical and biophysical research communications

authors： Barberis M,Pagano MA,Gioia LD,Marin O,Vanoni M,Pinna LA,Alberghina L

更新日期：2005-11-04 00:00:00

abstract：:Poly(ADP-ribose) polymerase 1 (PARP-1) is a nuclear enzyme that catalyzes the synthesis of ADP-ribose polymers from NAD(+). The function of PARP-1 is related to important nuclear processes including DNA repair and transcription. Previous studies demonstrated a specific physical interaction between PARP-1 and the trans...

journal_title：Biochemical and biophysical research communications

authors： Oei SL,Shi Y

更新日期：2001-06-08 00:00:00

abstract：:Aldose reductase has been purified to homogeneity from bovine retina. It has an apparent molecular weight of 32,000 daltons and shares immunological and kinetic properties with the much studied aldose reductases purified from various sources. Retinal aldose reductase displays a K(m) of approximately 40 microM with 4-h...

journal_title：Biochemical and biophysical research communications

authors： He Q,Khanna P,Srivastava S,van Kuijk FJ,Ansari NH

更新日期：1998-06-29 00:00:00

abstract：:The effect of phospholipids on IFN-gamma production in mouse T cells was investigated. Phosphatidylserine (PS), which has a negatively charged head group, completely inhibited IFN-gamma production in splenic naïve T cells and antigen-dependent IFN-gamma production in Th1 clone 42-6A cells, whereas other phospholipids,...

journal_title：Biochemical and biophysical research communications

authors： Yotsumoto S,Kakiuchi T,Aramaki Y

更新日期：2005-12-30 00:00:00

abstract：:ZinT (B1973), previously known as YodA, was originally characterised as a cadmium-induced periplasmic protein under the regulation of Fur and SoxS. Here we describe a decrease in zinT transcript in response to elevated copper concentrations and the zinc and copper dependent phenotype of a DeltazinT strain. Cadmium sen...

journal_title：Biochemical and biophysical research communications

authors： Kershaw CJ,Brown NL,Hobman JL

更新日期：2007-12-07 00:00:00

abstract：:p8 is an 80 amino-acid polypeptide identified because of its remarkable over-expression in the stressed pancreas. This protein, apparently devoid of enzymatic activity, is a powerful regulator of several intracellular pathways, suggesting that it has to interact with several molecular partners to modulate their activi...

journal_title：Biochemical and biophysical research communications

authors： Malicet C,Hoffmeister A,Moreno S,Closa D,Dagorn JC,Vasseur S,Iovanna JL

更新日期：2006-01-06 00:00:00

abstract：:It is well known that some intestinal bacteria, such as Escherichia coli, can produce a remarkable amount of molecular hydrogen (H(2)). Although the antioxidant effects of H(2) are well documented, the present study examined whether H(2) released from intestinally colonized bacteria could affect Concanavalin A (ConA)-...

journal_title：Biochemical and biophysical research communications

authors： Kajiya M,Sato K,Silva MJ,Ouhara K,Do PM,Shanmugam KT,Kawai T

更新日期：2009-08-21 00:00:00

abstract：:Ecdysone 20-monooxygenase activity and the factors which may regulate this steroid hydroxylase were examined in the midgut of the tobacco hornworm, Manduca sexta, during the last larval stadium. Radioassay experiments revealed that midgut ecdysone 20-monooxygenase undergoes a single 50-fold increase in activity tempor...

journal_title：Biochemical and biophysical research communications

authors： Keogh DP,Johnson RF,Smith SL

更新日期：1989-11-30 00:00:00

abstract：:NAD+ is a co-enzyme in redox reactions and a substrate required for activity of various enzyme families, including sirtuins and poly(ADP-ribose) polymerases. Dietary supplementation of NAD+ precursors nicotinamide mononucleotide (NMN) or nicotinamide riboside (NR) protects against metabolic disease, neurodegenerative ...

journal_title：Biochemical and biophysical research communications

authors： Fan R,Cui J,Ren F,Wang Q,Huang Y,Zhao B,Wei L,Qian X,Xiong X

更新日期：2018-06-02 00:00:00

abstract：:Rats were fed for 15 days a diet containing ammonium acetate (20% w/w) and then injected i. p. with ammonium acetate (7 mmol/Kg). Only 1 out of 18 control rats but 9 of 18 rats fed ammonium survived, indicating a protective effect of ammonium ingestion against an acute ammonia challenge. Blood ammonia returned to norm...

journal_title：Biochemical and biophysical research communications

authors： Miñana MD,Felipo V,Grisolía S

更新日期：1988-06-30 00:00:00

abstract：:We analyzed lesioned LDL in both atherosclerotic humans and in the apo E deficient (E degree) mice and compared its characteristics to plasma LDL. Lesioned LDL, in comparison to plasma LDL, was minimally oxidized and aggregated. Upon incubation of E degree-aortic lesions with 125[I]-labeled LDL, a time-dependent oxida...

journal_title：Biochemical and biophysical research communications

authors： Aviram M,Maor I,Keidar S,Hayek T,Oiknine J,Bar-El Y,Adler Z,Kertzman V,Milo S

更新日期：1995-11-13 00:00:00

abstract：:The extravascular localization of tissue factor (TF), the central initiator of coagulation, is thought to ensure that thrombus formation is prevented in the intact vessel. We observed that during a 5-min stimulation of human blood with collagen (type I), TF antigen appeared on the surface of platelets adhering to leuk...

journal_title：Biochemical and biophysical research communications

authors： Zillmann A,Luther T,Müller I,Kotzsch M,Spannagl M,Kauke T,Oelschlägel U,Zahler S,Engelmann B

更新日期：2001-02-23 00:00:00

abstract：:This article has been withdrawn at the request of the author. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. ...

journal_title：Biochemical and biophysical research communications

authors： Seong ES,Cho HS,Choi D,Joung YH,Lim CK,Hur JH,Wang MH

更新日期：2007-11-21 00:00:00

abstract：:Protein phosphatase 2Cs (PP2Cs) have been referred to act as negative modulators of the protein kinase pathways involved in different environmental stress responses and developmental processes. In Arabidopsis, PP2Cs have been extensively studied and some are known to negatively regulate abscisic acid signaling. In ric...

journal_title：Biochemical and biophysical research communications

authors： Amir Hossain M,Ahn CH,Lee IS,Jeon JS,An G,Park PB

更新日期：2018-09-10 00:00:00

abstract：:Coenzyme Q10 (CoQ10) protects retinal ganglion cells (RGCs) in experimental retinal ischemia and glaucoma by scavenging reactive oxygen species. We tested whether a diet supplemented with ubiquinol, the reduced form of CoQ10, promotes RGC survival and blocks the apoptotic pathway in ischemic mouse retina induced by ac...

journal_title：Biochemical and biophysical research communications

authors： Ju WK,Shim MS,Kim KY,Bu JH,Park TL,Ahn S,Weinreb RN

更新日期：2018-09-18 00:00:00

abstract：:Subcellular distribution of a novel annexin, annexin XI, was examined in rat tissues. As we have previously reported, annexin XI mainly localizes in nuclei of 3Y1, the rat embryonic fibroblast cell line; however, immunoperoxidase staining was not particularly nuclear in most of the tissues examined in adult rats. Nucl...

journal_title：Biochemical and biophysical research communications

authors： Mamiya N,Iino S,Mizutani A,Kobayashi S,Hidaka H

更新日期：1994-07-15 00:00:00

abstract：:Reactions of Paracoccus halodenitrificans nitric oxide reductase (NOR) containing four iron centers, a low spin hemec, a low spin heme b, a high spin heme b and a non-heme iron, have been studied to show the roles of each iron center. Soon after reacting the resting (oxidized) NOR with L-ascorbate, the low spin heme c...

journal_title：Biochemical and biophysical research communications

authors： Sakurai T,Sakurai N,Matsumoto H,Hirota S,Yamauchi O

更新日期：1998-10-09 00:00:00

abstract：:Alpha 1-acid glycoprotein (AGP) complementary DNA clone has been isolated from an acute phase rabbit liver library. Complete and full-length nucleotide sequence of this cDNA has been determined. The cDNA contains 35 nucleotides 5' untranslated region followed by 606 nucleotides of coding region and a 112 nucleotides 3...

journal_title：Biochemical and biophysical research communications

authors： Ray BK,Ray A

更新日期：1991-07-31 00:00:00

abstract：:Spermine and spermidine are present in rat liver mitochondria at the concentrations of 3.5 and 1.8 nanomoles/mg protein, respectively. Addition of 50 microM Ca2+ and 1 mM Pi to respiring mitochondria induces, concomitantly with mitochondrial swelling and Mg2+ efflux, a consistent release of polyamines, which is either...

journal_title：Biochemical and biophysical research communications

authors： Tassani V,Campagnolo M,Toninello A,Siliprandi D

更新日期：1996-09-24 00:00:00

abstract：:The poly(ADP-ribose) polymerase inhibitor 3-aminobenzamide had dramatically different effects on X-ray-induced cytogenetic damage in human lymphocytes depending on the stage of the cell cycle in which cells were irradiated. 3-Aminobenzamide (0.08-3.00 mM) potentiated the frequency of chromosomal aberrations when lymph...

journal_title：Biochemical and biophysical research communications

authors： Wiencke JK,Morgan WF

更新日期：1987-02-27 00:00:00

abstract：:ELOVL3 is involved in elongating saturated and monounsaturated fatty acids, and is a critical enzyme for lipid accumulation in brown adipocytes during the early phase of tissue recruitment. In addition, ELOVL3 is related to increased fatty acid oxidation in brown adipocytes. However, the potential functions of ELOVL3 ...

journal_title：Biochemical and biophysical research communications

authors： Liu D,Xu JH,Tong HL,Li SF,Yan YQ

更新日期：2017-12-02 00:00:00

abstract：:Dysfunction of Girdin plays a crucial role in the development of a variety of tumors. Phosphorylated regulation of Girdin has been studied extensively. However, how Girdin is dephosphorylated remains unclear. In this study, we report a mechanism of Girdin dephosphorylation and the importance of this mechanism in the m...

journal_title：Biochemical and biophysical research communications

authors： Li J,Enomoto A,Weng L,Sun L,Takahashi M

更新日期：2019-05-21 00:00:00

abstract：:Exocytosis is regulated by exocytotic proteins, which are present in insulin-secreting beta-cells and play regulatory roles in insulin secretion. Non-insulin dependent diabetes mellitus (type 2 diabetes) is a disease characterized by impaired insulin secretion and insulin resistance. Exocytotic protein immunoreactivit...

journal_title：Biochemical and biophysical research communications

authors： Zhang W,Khan A,Ostenson CG,Berggren PO,Efendic S,Meister B

更新日期：2002-03-08 00:00:00

abstract：:Small GTPase Rap1 has been implicated in the proper differentiation of testicular germ cells. In the present study, we investigated the functional significance of RA-GEF-2/Rapgef6, a guanine nucleotide exchange factor for Rap1, in testicular differentiation using mice lacking RA-GEF-2. RA-GEF-2 was expressed predomina...

journal_title：Biochemical and biophysical research communications

authors： Okada K,Miyake H,Yamaguchi K,Chiba K,Maeta K,Bilasy SE,Edamatsu H,Kataoka T,Fujisawa M

更新日期：2014-02-28 00:00:00

abstract：:Several studies have demonstrated that connexin 43 (Cx43) mediates signals important for osteoblast function and osteogenesis. The role of gap junctional communication in bone resorption is less clear. We have investigated the expression of Cx43 mRNA in osteoclasts and bone resorption cultures and furthermore, the fun...

journal_title：Biochemical and biophysical research communications

authors： Ransjö M,Sahli J,Lie A

更新日期：2003-04-18 00:00:00