Abstract：
:Gene targeting to disrupt gene expression in a temporal and spatial manner in a specific tissue using Cre recombinase-mediated gene inactivation has been proven to be useful to study in vivo gene function. To delete genes specifically in neurons during the late phase of brain development, we have generated transgenic mouse lines that express Cre recombinase under the control of the murine neurofilament-H (mNF-H) gene promoter. In this study, we report that one of these mouse lines expresses Cre recombinase specifically in the neurons of the brain and spinal cord during the late stage of their development. The transgenic line displays specific excision of the loxP-flanked gene in the neurons just after embryonic day 18.5 (E.18.5), which coincides with the later phase of brain maturation including spinal cord and olfactory bulb area. This mNF-H-cre transgenic mouse line will be valuable for studying in vivo functions of neuron-specific genes, particularly, defining their precise roles in the mature nervous system using conditional gene targeting strategies.
journal_name
Neurosci Resjournal_title
Neuroscience researchauthors
Hirasawa M,Cho A,Sreenath T,Sauer B,Julien JP,Kulkarni ABdoi
10.1016/s0168-0102(01)00216-4subject
Has Abstractpub_date
2001-06-01 00:00:00pages
125-32issue
2eissn
0168-0102issn
1872-8111pii
S0168010201002164journal_volume
40pub_type
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journal_title：Neuroscience research
pub_type： 杂志文章,评审
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journal_title：Neuroscience research
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Neuroscience research
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journal_title：Neuroscience research
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journal_title：Neuroscience research
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