Abstract：
:A glial cell line designated as B2 was generated from primary cultures of oligodendrocytes/astrocytes isolated from an adult BALB/c mouse brain and maintained for over 1 year. Phenotypic characteristics of B2 cells were investigated by immunolabeling with cell type-specific markers for oligodendrocytes (O4 and galactocerebroside (GalC)), astrocytes (glial fibrillary acidic protein (GFAP)), and immature neuroectodermal cells (vimentin). When cultured in a serum-containing medium, B2 cells exhibited a bipolar or a tripolar process-bearing morphology and proliferated with a 24-28 h doubling time, without requirement of exogenous growth factors. Under this culture condition, vimentin was identified in all of the B2 cells, GFAP in 7%, and O4 and GalC in less than 1% of the cells. When cultured in a serum-free medium containing 1 mM dibutyryl cyclic AMP (dbcAMP), B2 cells extended longer processes and 45% of the cells expressed cell type-specific markers for oligodendrocytes or astrocytes. GFAP was identified in 29% of B2 cells, O4 in 16%, and GalC in 6% of the cells, although, neither O4+GFAP+ nor GalC+GFAP+ cells were observed. B2 cells proliferated in response to phorbol 12-myristate 13-acetate (PMA), basic fibroblast growth factor (bFGF), insulin, insulin-like growth factor (IGF-I) and platelet-derived growth factor (PDGF), but not to dbcAMP, forskolin (FK), or retinoic acid (RA). These results indicate that B2 cells are distinct from typical oligodendrocytes and astrocytes with respect to their great proliferative potential, and suggest that B2 cells, with a capacity to differentiate into oligodendrocytes and astrocytes in response to cyclic AMP, may represent a population of glial precursor cells in the adult mouse central nervous system (CNS).
journal_name
Neurosci Resjournal_title
Neuroscience researchauthors
Satoh J,Tabira T,Kim SUdoi
10.1016/0168-0102(94)90035-3subject
Has Abstractpub_date
1994-08-01 00:00:00pages
175-84issue
2eissn
0168-0102issn
1872-8111pii
0168-0102(94)90035-3journal_volume
20pub_type
杂志文章abstract：:In the guinea-pig two subcortical structures have been shown to contain representations of auditory space, the deep layers of the superior colliculus (SC) and the external nucleus of the inferior colliculus (ICX). The following investigation was undertaken to determine if the ICX influences the development of the SC a...
journal_title：Neuroscience research
pub_type： 杂志文章
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更新日期：1996-07-01 00:00:00
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journal_title：Neuroscience research
pub_type： 杂志文章
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更新日期：2004-04-01 00:00:00
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journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/0168-0102(89)90004-7
更新日期：1989-06-01 00:00:00
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更新日期：1990-06-01 00:00:00
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pub_type： 杂志文章
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更新日期：2004-08-01 00:00:00
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journal_title：Neuroscience research
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更新日期：1998-11-01 00:00:00
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journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2008.03.007
更新日期：2008-07-01 00:00:00
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journal_title：Neuroscience research
pub_type： 杂志文章
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更新日期：1997-04-01 00:00:00
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journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2009.02.014
更新日期：2009-06-01 00:00:00
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journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/s0168-0102(97)00093-x
更新日期：1997-11-01 00:00:00
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journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2014.12.003
更新日期：2015-05-01 00:00:00
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journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/0168-0102(91)90042-w
更新日期：1991-08-01 00:00:00
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journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/0168-0102(92)90026-9
更新日期：1992-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/s0168-0102(97)00034-5
更新日期：1997-06-01 00:00:00
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journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2007.09.007
更新日期：2008-01-01 00:00:00
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journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2019.06.001
更新日期：2020-06-01 00:00:00
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journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/0168-0102(92)90047-g
更新日期：1992-12-01 00:00:00
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journal_title：Neuroscience research
pub_type： 杂志文章
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更新日期：1993-03-01 00:00:00
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journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/0168-0102(90)90009-4
更新日期：1990-02-01 00:00:00
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journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2006.02.014
更新日期：2006-06-01 00:00:00
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journal_title：Neuroscience research
pub_type： 杂志文章,评审
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更新日期：2020-07-01 00:00:00
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journal_title：Neuroscience research
pub_type： 杂志文章,评审
doi：10.1016/j.neures.2017.02.004
更新日期：2017-07-01 00:00:00
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journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2019.09.003
更新日期：2020-07-01 00:00:00
abstract：:Our previous research suggested that the P2X4 receptor (P2X4R) expression in microglia was involved in the activation of toll-like receptor-4 (TLR4) in the dorsal horn in the rat model of cancer induced bone pain (CIBP). In this study, we focused on whether TLR4- mitogen-activated protein kinases, p38 (p38 MAPK) contr...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2017.06.006
更新日期：2017-12-01 00:00:00
abstract：:The rostro-caudal distribution of luteinizing hormone-releasing hormone (LHRH) in the hypothalamus of neonatally estrogenized (10 micrograms EB for 1-5 days), sterile female rats was compared with that of cycling rats at 50, 100 and 240 days of age. Serial slices of 400 microns in thickness in total were cut from a hy...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/0168-0102(91)90004-i
更新日期：1991-10-01 00:00:00
abstract：:A significant activity of [3H]glutamate binding was detected in homogenate particulate preparations obtained from the rat pituitary, in addition to central structures including the cerebral cortex. In contrast to the cerebral binding, the pituitary binding was significantly inhibited by Na+ ions. It was also found tha...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/0168-0102(86)90034-9
更新日期：1986-07-01 00:00:00
abstract：:The subiculum is one of output structures of the hippocampal formation and plays a pivotal role in learning and memory. Because its morphological features are less investigated than those of the hippocampus proper, we explored the internal structure of the rat subiculum using immunohistochemistry. The septal subiculum...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/j.neures.2019.02.001
更新日期：2020-01-01 00:00:00
abstract：:GABAergic neurons in the hippocampus proper are greatly diverse in their morphological and physiological features. In the present study we examined whether or not they are also diverse regarding the density of GABAergic input on their somata. GABAergic neurons were immunocytochemically identified with antibodies again...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/s0168-0102(96)01102-9
更新日期：1996-10-01 00:00:00
abstract：:Mature rat spinal cord cDNA libraries constructed in lambda gt10 and lambda ZAPII were screened with an oligonucleotide probe (39 mer), and 4 clones that possess DNA-inserts encoding a glycine receptor subunit were obtained. The cloned cDNAs were used to reconstruct the nucleotide sequence of the full-length open read...
journal_title：Neuroscience research
pub_type： 杂志文章
doi：10.1016/0168-0102(91)90064-6
更新日期：1991-06-01 00:00:00
