Abstract:
:Confocal laser scanning microscopy (CLSM) was used to record spatial and dynamic changes in the intracellular Ca2+ [(Ca2+]i) of bullfrog sympathetic ganglion cells in excised tissue or in culture. A CLSM utilizing Ar ion laser (488 nm) and recording fluo-3 fluorescence yielded the sliced image of ganglion cells, while conventional epifluorescence microscopy provided the cell image of a convex structure. A high K+ (50 mM) solution, caffeine (3-10 mM) and electrical stimulation (10-20 Hz, 0.5-10 s) caused a homogeneous increase in fluo-3 fluorescence with or without regional differences, possibly due to intracellular organelles and other constituents. Scanning a single horizontal line across the cytoplasm with He-Cd laser (325 nm) and recording indo-1 fluorescence demonstrated that the rate of rise in [Ca2+]i following action potentials depends on the distance from the cell membrane and on the cytoplasmic constituents, showing an inward spread of 'Ca(2+)-wave' at variable speeds of 17-219 microns/s. These results suggest that heterogeneity of the cytoplasmic structures and constituents affects dynamic and spatial changes of [Ca2+]i in response to stimuli in neurones. Such heterogenic changes in [Ca2+]i would better be studied by CLSM.
journal_name
Neurosci Resjournal_title
Neuroscience researchauthors
Kuba K,Hua SY,Nohmi Mdoi
10.1016/0168-0102(91)90082-asubject
Has Abstractpub_date
1991-05-01 00:00:00pages
245-59issue
4eissn
0168-0102issn
1872-8111pii
0168-0102(91)90082-Ajournal_volume
10pub_type
杂志文章abstract::The chemical properties of probes that improve amyloid detection by non-invasive (19)F magnetic resonance imaging (MRI) are of interest. We synthesized benzoxazole compounds with trifluoromethoxy groups, and found that these compounds displayed sharp (19)F nuclear magnetic resonance (NMR) signals in an assay buffer. H...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/j.neures.2008.10.006
更新日期:2009-01-01 00:00:00
abstract::Growth hormone (GH) is a pleiotropic hormone that exerts important functions in the control of brain development as well as in the regulation neuronal differentiation and function, together with several behavioral and psychological effects that have been linked to its modulatory actions on brain neurotransmitters. In ...
journal_title:Neuroscience research
pub_type: 杂志文章,评审
doi:10.1016/j.neures.2013.03.014
更新日期:2013-08-01 00:00:00
abstract::This study was designed to evaluate the anticonvulsant effects of imperatorin (a furanocoumarin isolated from fruits of Angelica archangelica) in the mouse maximal electroshock seizure threshold model. The threshold for electroconvulsions in mice was determined at several times: 15, 30, 60 and 120 min after i.p. admin...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/j.neures.2007.05.004
更新日期:2007-09-01 00:00:00
abstract::Men and women exhibit differences in sexual behavior. This indicates that neural circuits within the central nervous system (CNS) that control sexual behavior differ between the sexes, although differences in behavior are also influenced by sociocultural and hormonal factors. Sexual differentiation of the body and bra...
journal_title:Neuroscience research
pub_type: 杂志文章,评审
doi:10.1016/j.neures.2011.11.002
更新日期:2012-02-01 00:00:00
abstract::How the nervous system controls the sensation and memory of information from the environment is an essential question. The nematode Caenorhabditis elegans is a useful model for elucidating neural information processing that mediates sensation and memory. The entire nervous system of C. elegans consists of only 302 neu...
journal_title:Neuroscience research
pub_type: 杂志文章,评审
doi:10.1016/j.neures.2013.03.008
更新日期:2013-07-01 00:00:00
abstract::Avian cochlear neurons of the nucleus magnocellularis (NMC) are known to encode temporal information of sound. The neuron generated only a single action potential at a stable timing even though suprathreshold currents of long duration (> 100 ms) was injected. The threshold for the action potential was -42 mV. In volta...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/0168-0102(96)01071-1
更新日期:1996-09-01 00:00:00
abstract::Over the last few decades, motor imagery has attracted the attention of researchers as a prototypical example of 'embodied cognition' and also as a basis for neuro-rehabilitation and brain-machine interfaces. The current definition of motor imagery is widely accepted, but it is important to note that various abilities...
journal_title:Neuroscience research
pub_type: 杂志文章,评审
doi:10.1016/j.neures.2015.11.003
更新日期:2016-03-01 00:00:00
abstract::The influence of the phosphorylation dephosphorylation states on the gamma-aminobutyric acid (GABA) transporter activity of synaptic plasma membranes (SPM) was studied by using either specific phosphatase inhibitors or activators. Calyculin A and okadaic acid (phosphatase 1 and phosphatase 2A inhibitors) inhibited the...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/s0168-0102(98)00107-2
更新日期:1999-01-01 00:00:00
abstract::Developmental refinement of neuronal connectivity is crucial for proper brain function. In the early phase of development, input fibers arrive at their target areas guided by specific molecular cues and form abundant immature synapses. Then, functionally important synapses are preserved and strengthened by neural acti...
journal_title:Neuroscience research
pub_type: 杂志文章,评审
doi:10.1016/j.neures.2017.06.004
更新日期:2018-04-01 00:00:00
abstract::Axonal transport is the specialized and well-developed intracellular transport system for regulated and/or long-distance transport based on generalized cellular machineries. Among them, slow axonal transport conveys cytoplasmic proteins. The motor molecule, the nature of transporting complex and the transport regulati...
journal_title:Neuroscience research
pub_type: 杂志文章,评审
doi:10.1016/j.neures.2003.08.005
更新日期:2003-12-01 00:00:00
abstract::The neuroprotective effect of nicotine via nicotinic acetylcholine receptor (nAChR) has been reported in differentiated PC12 cells. We examined the effects of nerve growth factor (NGF) and nicotine on the expressions of the nAChR subunits alpha3, alpha5, alpha7, beta2, beta3 and beta4, in PC12 cells using Northern blo...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/s0168-0102(99)00064-4
更新日期:1999-12-01 00:00:00
abstract::The present study focused on the early stages of acute glutamate (L-Glu)-induced neurotoxic mechanisms, both biochemical, e.g. intracellular reactive oxygen species (ROS) and associated parameters as well as gene expression of cell survival/death pathways, i.e. Bcl-2 and caspases. Stereotactic intracortical injections...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/j.neures.2009.11.009
更新日期:2010-03-01 00:00:00
abstract::Accumulating evidence demonstrates astrocytic crucial roles in the brain, but the molecular basis underlying astrocytic intracellular protein trafficking remains to be elucidated. The present study reports the identification of novel protein, brain-derived integrating factor-1 (BDIF1), which comprises TBC (Tre-2/Bub2/...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/j.neures.2011.03.008
更新日期:2011-07-01 00:00:00
abstract::Eye movements were induced by stimulating the ventral bank of the anterior ectosylvian sulcus (AES) of chloralose-anesthetized cats. Intracortical microstimulation of this cortex evoked centering movements of both eyes. The latency of eye movement was 45 ms (range 40-60 ms) and the intensity of stimulation was 33 micr...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/0168-0102(89)90056-4
更新日期:1989-11-01 00:00:00
abstract::We investigated the distribution and colocalization pattern of the two corticosteroid receptors, mineralocorticoid receptor (MR) and glucocorticoid receptor (GR), in the hippocampus and hypothalamus, the main target regions of corticosterone in the rat brain, using double immunofluorescence histochemistry in conjuncti...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/j.neures.2004.12.013
更新日期:2005-04-01 00:00:00
abstract::Knowledge remains limited about how chronic cathinone exposure impacts dopamine systems in brain reward circuits. In the present study, a binge-like MDPV exposure that impaired novel object recognition (NOR) dysregulated dopamine markers in mesocorticolimbic substrates of rats, with especially profound effects on D1 a...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/j.neures.2019.07.003
更新日期:2020-06-01 00:00:00
abstract::The coexistence of varying combinations of substance P (SP), somatostatin (SOM), thyrotropin-releasing hormone (TRH) and met-enkephalin-Arg-Gly-Leu (ENK) with 5-hydroxytryptamine (5-HT) as semiquantitatively revealed by immunocytochemistry in neuronal perikarya of the raphe pallidus et obscurus in the guinea-pig was a...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/0168-0102(89)90033-3
更新日期:1989-10-01 00:00:00
abstract::Speech prosody is considered to be one of the most important sources of information for infants in acquiring their native language. Using multi-channel near-infrared spectroscopy in 10-month-old infants, we examined cortical activation when normal and flattened speech sounds were presented to the infants. The flattene...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/j.neures.2007.05.005
更新日期:2007-09-01 00:00:00
abstract::Slow disjunctive eye movement similar to ocular convergence was evoked by microstimulation in parts of the lateral suprasylvian area (LSA) in alert cats. A tungsten-in-glass microelectrode was used for stimulation, and eye movement was monitored using the magnetic search coil method. The velocity-versus-amplitude rela...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/0168-0102(91)90120-n
更新日期:1991-10-01 00:00:00
abstract::The occurrence of multiple proteoglycan species is a characteristic of the brain. The structural features of individually characterized proteoglycans in the brain are first introduced in brief, then some examples are shown that suggest a relationship between multiple proteoglycans and the many distinct cell types and ...
journal_title:Neuroscience research
pub_type: 杂志文章,评审
doi:10.1016/0168-0102(94)90088-4
更新日期:1994-09-01 00:00:00
abstract::Although the rat has been the predominant rodent used to investigate the pathophysiology and treatment of experimental spinal cord injury (SCI), the increasing availability of transgenic animals has led to greater use of mouse models. However, behavioral assessment after SCI in mice has been less extensively investiga...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/j.neures.2010.02.009
更新日期:2010-06-01 00:00:00
abstract::We investigated the neural mechanisms underlying the ability to cope in atypical or novel situations using tools. We hypothesized that two cognitive components support this ability: adaptive coordination (for adapting to situational demands) and cognitive inhibition (for inhibiting the incongruent actions afforded by ...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/j.neures.2014.03.008
更新日期:2015-01-01 00:00:00
abstract::In the guinea-pig two subcortical structures have been shown to contain representations of auditory space, the deep layers of the superior colliculus (SC) and the external nucleus of the inferior colliculus (ICX). The following investigation was undertaken to determine if the ICX influences the development of the SC a...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/0168-0102(96)01060-7
更新日期:1996-07-01 00:00:00
abstract::The cerebellar Purkinje cell has highly branched dendrites extending in a transverse plane to the cerebellar folium. Branching patterns of the dendrites were subjected to fractal analysis to obtain a quantitative measure of their intricacy. Using a square-covering method, we found that dendrites have a statistical sel...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/0168-0102(92)90031-7
更新日期:1992-02-01 00:00:00
abstract::To investigate the representation of facial skin areas in the primary somatosensory cortex (SI), we recorded magnetic fields evoked by air pressure-induced tactile stimulation applied to six points on the face, lower lip and thumb. The thumb area in the SI was located more medial and superior to the lip area, which wa...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/j.neures.2004.07.004
更新日期:2004-10-01 00:00:00
abstract::Previous pharmacological experiments provide conflicting findings that describe both facilitatory and inhibitory effects of neuronal histamine on learning and memory. Here, we examined learning and memory and synaptic plasticity in mice with a null mutation of gene coding histamine H1 or H2 receptor in order to clarif...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/j.neures.2006.10.020
更新日期:2007-02-01 00:00:00
abstract::Most imaging studies of the enteric nervous system (ENS) that regulates the function of the gastrointestinal tract are so far performed using preparations isolated from animals, thus hindering the understanding of the ENS function in vivo. Here we report a method for imaging the ENS cellular network activity in living...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/j.neures.2019.02.004
更新日期:2020-02-01 00:00:00
abstract::In acute decerebrate cats, medial pontine reticular formation (mPRF) and the mesencephalic locomotor region (MLR) were stimulated and their stimulus effects upon 250 medullary reticulospinal neurons (RSNs) were studied. One hundred and twenty-six RSNs were mono- and disynaptically activated. From the response patterns...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:
更新日期:1995-08-01 00:00:00
abstract::The interaction of neurotransmitter receptors with the underlying cytoskeleton via subsynaptic proteins is an important mechanism for the targeting of the receptors to synapses in the central nervous system. We show that delta glutamate receptors (delta receptors), expressed predominantly in the dendritic spines of ce...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/s0168-0102(99)00061-9
更新日期:1999-09-01 00:00:00
abstract::Alcoholism is a psychiatric condition that develops through neuroadaptations in response to neuronal stresses caused by chronic ethanol intake. Neurons can adapt to ethanol-induced metabolic changes by activating cellular protective mechanisms, including autophagy. Here we show that expression of Ulk1, a gene critical...
journal_title:Neuroscience research
pub_type: 杂志文章
doi:10.1016/j.neures.2016.12.004
更新日期:2017-04-01 00:00:00