Abstract:
:We recently showed that MMP-9 activity was detectable in the cerebrospinal fluid (CSF) of about half of neurologically symptomatic HIV-infected patients. Using an experimental animal model, we detected MMP-9 activity in CSF samples from rats that had been injected intracisternally with recombinant HIV-1 Nef protein, but not after injection of heat-treated Nef, gp120, gp160 or PBS. Nef also induced a breaching of the blood-brain barrier (BBB), which could be inhibited by pretreatment with the matrix metalloproteinase (MMP) inhibitor batimastat. In vitro Nef only slightly induced MMP-9 activity in freshly isolated human peripheral blood mononuclear cells and in the murine macrophage cell line RAW 264.7, but not in endothelial, neuronal or astroglial cell lines. Taken together, our findings indicate that HIV-1 Nef protein can induce BBB disruption in the rat - presumably via MMP induction.
journal_name
J Neuroimmunoljournal_title
Journal of neuroimmunologyauthors
Sporer B,Koedel U,Paul R,Kohleisen B,Erfle V,Fontana A,Pfister HWdoi
10.1016/s0165-5728(99)00170-8subject
Has Abstractpub_date
2000-01-24 00:00:00pages
125-30issue
2eissn
0165-5728issn
1872-8421pii
S0165572899001708journal_volume
102pub_type
杂志文章abstract::The expression of several cell type-specific markers was studied by indirect immunofluorescence in discontinuous BSA gradient fractionated and unfractionated mouse cerebellar cells cultured for 3 days in vitro from embryonic day 13 through postnatal day 9. Cell surface antigen NS-4 and tetanus toxin receptors are pres...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(81)90024-2
更新日期:1981-12-01 00:00:00
abstract::An electroimmunoblotting technique was used with a monoclonal antibody to myelin basic protein (MBP) to assess demyelination in 3 defined regions of the spinal cord in rats with acute experimental autoimmune encephalomyelitis (EAE). A slight loss in MBP was detected only in the sacrococcygeal region of the spinal cord...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(85)80035-7
更新日期:1985-10-01 00:00:00
abstract::Experimental autoimmune neuritis (EAN) is an animal model for Guillain-Barré syndrome (GBS). Intravenous immunoglobulins (IVIg) are an effective treatment for GBS, but their mechanism of action is not well understood. Here we tested whether IVIg treatment, a potent modulator of proinflammatory assaults, reduces inflam...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(97)00039-8
更新日期:1997-06-01 00:00:00
abstract::We investigated in multiple sclerosis the difference between two commonly used oral and intravenous steroid regimens on the level of adhesion molecule expression on blood T lymphocytes, the distribution of circulating T cell subsets, and the concentration of serum tumour necrosis factor alpha. Venous blood samples wer...
journal_title:Journal of neuroimmunology
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1016/s0165-5728(96)00206-8
更新日期:1997-04-01 00:00:00
abstract::Lewis (LEW) and DA rats are highly susceptible to experimental autoimmune encephalomyelitis (EAE) induced with guinea pig myelin basic protein (MBP), but respond to different epitopes. The dominant epitope for LEW rats is MBP73-86, and disease is mediated primarily by Vbeta8.2 Th1 cells. DA rats lack conventional Vbet...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(01)00244-2
更新日期:2001-03-01 00:00:00
abstract::We have analyzed the impact of stroke with subsequent hemiparesis and sensory loss on in vivo mediated immune functions. The delayed-type hypersensitivity (DTH) reaction to purified protein derivate (PPD, tuberculin) was used as a measure of antigen-specific T-cell reactivity, and subcutaneous immunization with influe...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(91)90099-s
更新日期:1991-10-01 00:00:00
abstract::The NF-κB signalling pathway plays an important role in controlling cellular immune responses, inflammation and apoptosis. In multiple sclerosis (MS), there is evidence of dysregulation of NF-κB signalling in patients with a relapsing-remitting disease course, but thus far there is little information on whether it is ...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2018.04.002
更新日期:2018-07-15 00:00:00
abstract::The present work examined the effects induced by dibutyryl cyclic adenosine monophosphate (dB-cAMP) on microglial cells in primary glial cell cultures from newborn rats. Microglial cells were identified by OX42 immunohistochemistry and nucleoside diphosphatase histochemistry. Double staining for astrocytes was carried...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(96)00074-4
更新日期:1996-11-01 00:00:00
abstract::Sprague-Dawley rats were injected intraperitoneally with a suspension of Trypanosoma brucei brucei. An early induction of major histocompatibility complex (MHC) class I antigens as well as an infiltration of macrophage-like cells and cytotoxic T-cells was detected with immunohistochemical techniques in circumventricul...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(89)90104-5
更新日期:1989-09-01 00:00:00
abstract::Insulin-like growth factor-2 (IGF-2) present in media conditioned by non-activated and interferon gamma (IFN gamma)-treated microglia reduces galactocerebroside(+) (GalC) oligodendrocyte apoptosis in cultures derived both from the CG4 cell line and primary rat cortices. Microglia-derived IGF-2 also acts in each cultur...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(02)00011-5
更新日期:2002-03-01 00:00:00
abstract::Reactive oxygen species (ROS) produced by activated microglia are deleterious to neurons. In this study, we studied the effect of Mn(III)tetrakis(1-methyl-4-pyridyl)porphyrin (MnTMPyP), a superoxide dismutase/catalase mimetic, on the lipopolysaccharide (LPS)-induced degeneration of dopaminergic neurons in rat mesencep...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2003.10.040
更新日期:2004-02-01 00:00:00
abstract::In this study, experimental allergic encephalomyelitis (EAE) rats and rats exhibiting EAE expressing high circulating anti-nerve growth factor antibody were daily monitored for clinical signs and chronic relapses. Eighty-five days after EAE induction, blood, spinal cord and brain stem were used for histological examin...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(99)00272-6
更新日期:2000-05-01 00:00:00
abstract::Polymyositis (PM) is an inflammatory muscle disease caused by autoimmune dysfunction, considered to be caused by cytotoxic CD8 T cells. To date, no autoantigens have been identified. We attempted to induce an experimental allergic myositis (EAM) in BALB/c mice by inoculating syngeneic dendritic cells (DC) presenting p...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(01)00327-7
更新日期:2001-07-02 00:00:00
abstract::This study reports on the immunocytochemical localization of tumor necrosis factor-alpha (TNF alpha) in immune-mediated demyelination and Wallerian degeneration of the rat peripheral nervous system (PNS) using teased nerve fiber preparations. In experimental autoimmune neuritis induced by active immunization (EAN) or ...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(93)90178-2
更新日期:1993-06-01 00:00:00
abstract::Using congenic strains of mice susceptible (bcg(s)) or resistant (bcg(r)) to BCG, murine microglial cell lines, RR4.R (BCG-resistant) and RR8.S (BCG-susceptible), were established in vitro. Comparative studies revealed that, although phagocytic to a similar extent, RR4.R cells were more active than RR8.S cells in term...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(99)00127-7
更新日期:1999-11-01 00:00:00
abstract::CD4+CD25+ regulatory T cells (Treg) transform the HIV-1 infected macrophage from a neurotoxic to a neuroprotective phenotype. This was demonstrated previously in a murine model of HIV-1 encephalitis induced by intracranial injection of HIV-1/vesicular stomatitis virus-infected bone marrow macrophages. In this report, ...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2010.08.014
更新日期:2011-01-01 00:00:00
abstract::Programmed death-1 (PD-1) belongs to the CD28 family of co-stimulatory and co-inhibitory molecules and regulates adaptive immunity. This molecule induces the development of regulatory T cells, T cell tolerance, or apoptosis. We examined the role of PD-1 pathway in Theiler's murine encephalomyelitis virus (TMEV)-induce...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2014.06.018
更新日期:2014-09-15 00:00:00
abstract::We evaluated the association of the plasma platelet-activating factor acetylhydrolase (PAF-AH) gene polymorphism (G(994)-->T) and PAF-AH activity with susceptibility and severity of multiple sclerosis (MS) in Japanese. DNA was collected from 216 patients with clinically definite MS (65 opticospinal MS (OS-MS) and 151 ...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2004.01.008
更新日期:2004-05-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF) is a key regulator of angiogenesis. The iron-chelator desferrioxamine (DFX) increased the expression of hypoxia-inducible factor (HIF)-1alpha in the hair cell line, HEI-OC1. The increased VEGF production by DFX was inhibited by iron. DFX also induced the activation of mitogen-...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2005.02.019
更新日期:2005-06-01 00:00:00
abstract::The blood brain barrier (BBB) and the blood-cerebrospinal fluid barrier (BCB) limit the influx of immune mediators and bloodstream compounds into the central nervous system (CNS). Upon injury or infection, the integrity of these barriers is compromised and leukocyte infiltration occurs. The BCB is located in the choro...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2007.05.005
更新日期:2007-07-01 00:00:00
abstract::To assess whether TNF-alpha causes inflammatory demyelination or axonal degeneration, we injected into rat sciatic nerve saline, 100 U and 1000 U of rhTNF-alpha and studied the electrophysiological and pathological effects. At day 1 electrophysiology showed a slight reduction of proximal compound muscle action potenti...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(98)00229-x
更新日期:1999-02-01 00:00:00
abstract::In this study we evaluated the percentages of CD3(-)CD56(bright), CD3(-)CD56(dim), CD3(-)CD56(bright)perforin(+) and CD3(-)CD56(dim)perforin(+) Natural Killer (NK) cells in peripheral blood from untreated secondary progressive (SP) and primary progressive (PP) multiple sclerosis (MS) patients and age and sex matched h...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2013.10.004
更新日期:2013-12-15 00:00:00
abstract::Bone marrow stromal cells (BMSCs) are strong candidates for cell therapy against human autoimmune diseases. Intravenous administration of syngenic BMSCs to EAMG-model rats effectively ameliorated the disease, partially through a TGF-beta-dependent mechanism. The proliferative ability of T or B cells from EAMG rats was...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2008.12.005
更新日期:2009-02-15 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) can induce a robust immune response initially thru the activation of pattern recognition receptors and subsequent type I interferon production that then shapes, along with other innate immune components, the adaptive immune response to the insult. While this response is necessary to...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章,评审
doi:10.1016/j.jneuroim.2009.09.013
更新日期:2010-03-30 00:00:00
abstract::To clarify the origin and function of the microglia residing in the central nervous system, we cloned brain cells from newborn and adult mice in soft agar containing the macrophage-specific growth factor, colony-stimulating factor-1 and expanded the cells from individual colonies in liquid culture medium. The results ...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(92)90071-r
更新日期:1992-12-01 00:00:00
abstract::A novel triptolide derivative (5R)-5-hydroxytriptolide (LLDT-8) has been shown to have potent immunosuppressive activities. Here LLDT-8 was evaluated in experimental autoimmune encephalomyelitis (EAE), the model of multiple sclerosis (MS). LLDT-8 reduced the incidence and severity of EAE, which was associated with the...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2006.03.011
更新日期:2006-06-01 00:00:00
abstract::Neuronal damage mediated by the TRAIL-system might be involved in the pathogenesis of neuroinflammatory diseases of the central nervous system. Here we used an investigator-independent approach to quantify TRAIL-mediated death of total CNS cells and neurons in a living human brain slice culture system, a model which i...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2005.06.027
更新日期:2005-10-01 00:00:00
abstract::The extent to which symptomatic improvement in patients with multiple sclerosis treated with intravenous methylprednisolone depends on immunological actions of corticosteroids is unknown. In this study the effect of methylprednisolone on circulating T and B cell function has been assessed in vitro and in vivo. Low mol...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(90)90114-3
更新日期:1990-01-01 00:00:00
abstract::Sympathetic nervous system (SNS) activation mobilizes blood leukocytes. Under these circumstances, both epinephrine (EPI) and neuropeptide Y (NPY) are released. Therefore, we investigated a possible interaction between these transmitters during leukocyte mobilization, using intravenous catheterization of male adult Le...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(02)00278-3
更新日期:2002-11-01 00:00:00
abstract::The role of autoantibody responses in pathogenesis or progression of multiple sclerosis (MS) remains controversial. This is partly because the methods that can distinctly identify pathogenic antibody reactivities targeting native membrane proteins from the reactivities that originate as an epiphenomenon in such diseas...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2010.02.008
更新日期:2010-04-15 00:00:00