PhTx4, a new class of toxins from Phoneutria nigriventer spider venom, inhibits the glutamate uptake in rat brain synaptosomes.

Abstract:

:We report the characterization of a new class of glutamate uptake inhibitors isolated from Phoneutria nigriventer venom. Glutamate transport activity was assayed in rat cerebrocortical synaptosomes by using [(3)H]-L-glutamate. PhTx4 inhibited glutamate uptake in a dose dependent manner. The IC(50) value obtained was 2.35+/-0.9 microg/ml which is in the observed range reported for glutamate uptake blockers. Tx4-7, one of PhTx4 toxins, showed the strongest inhibitory activity (50.3+/-0.69%, n=3).

journal_name

Brain Res

journal_title

Brain research

authors

Mafra RA,Figueiredo SG,Diniz CR,Cordeiro MN,Cruz JD,De Lima ME

doi

10.1016/s0006-8993(99)01472-9

subject

Has Abstract

pub_date

1999-06-12 00:00:00

pages

297-300

issue

1-2

eissn

0006-8993

issn

1872-6240

pii

S0006899399014729

journal_volume

831

pub_type

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