Naloxone antagonism of GABA-evoked membrane polarizations in cultured mouse spinal cord neurons.


:Pharmacological studies using an in vitro model system were carried out to determine if naloxone, an opiate receptor antagonist, could have effects on neuronal membranes which were unrelated to its action as an opiate receptor antagonist. Intracellular recordings were made from cultured mammalian spinal cord neurons. Putative amino acid neurotransmitters and naloxone were applied by iontophoresis or superfusion. When naloxone was co-iontophoresed with the amino acids a depression of the GABA response resulted. This depression was dose-dependent and reversible. At the lower doses of naloxone tested, the depression was specific since the glycine and glutamate responses were unaffected. At the higher doses of naloxone tested, alterations in the glycine and/or glutamate responses and membrane input resistance were frequently observed. The naloxone depression of the GABA response did not appear to involve opiate receptors since (+)-naloxone, the inactive isomer, equally depressed the GABA response. Analysis of the effect of naloxone on GABA dose-response curves indicates that naloxone acts as a competitive antagonist at the neuronal GABA receptors. Similar results were obtained when naloxone was applied by superfusion. However, high concentrations of naloxone (0.1-1 mM) were required, suggesting that naloxone has a low affinity for the GABA receptor. These data indicate that under some experimental conditions naloxone could not be considered a specific opiate antagonist.


Brain Res


Brain research


Gruol DL,Barker JL,Smith TG




Has Abstract


1980-10-06 00:00:00














  • The neurotoxic actions of ibotenic acid on cholinergic and opioid peptidergic systems in the central nervous system of the rat.

    abstract::The neurotoxic effects produced by ibotenic acid (IA) induced chemical lesions of the central nervous system (CNS) cholinergic system were examined on the opioid peptidergic system in adult rats. Forebrain cholinergic systems were bilaterally lesioned by the infusion of IA (1 or 5 micrograms/site) into the nucleus bas...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Rattan AK,Tejwani GA

    更新日期:1992-02-07 00:00:00

  • Co-localization of substance P and Met-enkephalin-Arg6-Gly7-Leu8 in the intraspinal neurons of the rat, with special reference to the neurons in the substantia gelatinosa.

    abstract::A double-labeling immunofluorescence technique was employed to investigate the co-localization of the functionally antagonistic neuropeptides, substance P and enkephalins, within intraspinal neurons of the rat. Anti-Met-enkephalin-Arg6-Gly7-Leu8 (Enk-8) antiserum was used as a marker of the preproenkephalin A neuron s...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Senba E,Yanaihara C,Yanaihara N,Tohyama M

    更新日期:1988-06-21 00:00:00

  • Sodium dodecylsulphate induces a breach in the blood-brain barrier and enables a West Nile virus variant to penetrate into mouse brain.

    abstract::A novel and convenient assay was used to determine the effect of recombinant Interleukin-2 (IL-2) on the function of the blood-brain barrier (BBB). The assay is based on a variant of the West Nile virus, WN-25, which had lost its neuroinvasiveness but not its neurovirulence. WN-25, when injected intravenously, can cau...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Kobiler D,Lustig S,Gozes Y,Ben-Nathan D,Akov Y

    更新日期:1989-09-04 00:00:00

  • Membrane structure in cultured astrocytes.

    abstract::Astrocytes cultured from the brains of neonatal rat pups acquire at least two specializations of intramembrane particle distribution: 'assemblies' and gap junctions. The number and appearance of assemblies in the cultured astrocytes is not markedly influenced by the presence or absence of a collagen substrate, and the...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Landis DM,Weinstein LA

    更新日期:1983-10-03 00:00:00

  • Response to an itch-producing substance in cat. I. Cutaneous receptor populations with myelinated axons.

    abstract::The purpose of this study was to determine which myelinated cutaneous receptor populations in cat are responsive to a stimulus (cowhage) that produces unambiguous pruritus in human subjects. Initial experiments using electrocutaneous stimuli demonstrated that fibers conducting as slowly as 1.5 m/s could be recorded fr...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Tuckett RP,Wei JY

    更新日期:1987-06-09 00:00:00

  • Changes in proteolytic enzymes and proteins during maturation of the brain.

    abstract::(1) Changes during development in the levels of proteinases and peptidases were measured in brain homogenates. At all ages di- and tripeptidase levels were 7-15-fold higher than proteinase activity. (2) Cathepsin A and D and neutral proteinase activity first decreased (during the 5 days before birth) and then increase...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Marks N,Stern F,Lajtha A

    更新日期:1975-03-21 00:00:00

  • Specificity of monoclonal antibody N1 for cell surfaces of mouse central nervous system neurons.

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    journal_title:Brain research

    pub_type: 杂志文章


    authors: Schnitzer J,Kim SU,Schachner M

    更新日期:1984-07-01 00:00:00

  • Embryonic neural transplants across a major histocompatibility barrier: survival and specificity of innervation.

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    journal_title:Brain research

    pub_type: 杂志文章


    authors: Low WC,Lewis PR,Terri ST

    更新日期:1983-03-07 00:00:00

  • Commissural and ipsilateral internuclear connection of vestibular nuclear complex of the cat.

    abstract::Commissural and ipsilateral intrinsic connections of the vestibular nuclear complex of cats were investigated using retrograde transport of horseradish peroxidase (HRP). HRP was microiontophoretically injected into limited areas (0.2-0.5 mm in diameter) of the respective vestibular nuclei. In the commissural connectio...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Ito J,Matsuoka I,Sasa M,Takaori S

    更新日期:1985-08-19 00:00:00

  • Editorial: Mechanistic underpinnings of stem cell therapy for neurological disorders.

    abstract::This special issue entitled "Mechanistic underpinnings of stem cell therapy for neurological disorders" brings together academicians, clinicians and industry partners with vested interest in the safe and effective translation of stem cell-based therapeutics from the laboratory to the clinic. Despite the scientific adv...

    journal_title:Brain research

    pub_type: 社论


    authors: Bonsack B,Kingsbury C,Brown J,Borlongan CV

    更新日期:2020-02-15 00:00:00

  • MPP+-induced increases in extracellular potassium ion activity in rat striatal slices suggest that consequences of MPP+ neurotoxicity are spread beyond dopaminergic terminals.

    abstract::MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) produces symptoms similar to idiopathic Parkinson's disease in primates. A metabolite of MPTP, MPP+ (1-methyl-4-phenylpyridinium), is actively accumulated by dopaminergic (DA) terminals and selectively destroys nigrostriatal DA neurons. The mechanism of this effect r...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Hollinden GE,Sanchez-Ramos JR,Sick TJ,Rosenthal M

    更新日期:1988-12-20 00:00:00

  • Serotonin increases the frequency of light-elicited discrete waves in type-B photoreceptors of Hermissenda.

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    journal_title:Brain research

    pub_type: 杂志文章


    authors: Crow T,Pengidore T

    更新日期:1993-11-19 00:00:00

  • Effect of cyclic nucleotide derivatives on the release of ACh from cortical slices of the rat brain.

    abstract::Atropine (5 nM to 5 muM), but not D-tubocurarine or hexamethonium, enhanced the release of acetylcholine (ACh) from rat brain cortical slices exposed to high K+. The enhancement was dose-dependent, and it could be partially antagonized by oxotremorine (50-500 muM) or by dibutyryl or 8-bromo-cyclic GMP (1 mM), but not ...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Yonehara N,Matsuda T,Saito K,Ishida H,Yoshida H

    更新日期:1980-01-20 00:00:00

  • Different epileptogenic activities of murine and ovine corticotropin-releasing factor.

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    journal_title:Brain research

    pub_type: 杂志文章


    authors: Marrosu F,Mereu G,Fratta W,Carcangiu P,Camarri F,Gessa GL

    更新日期:1987-04-07 00:00:00

  • Effects of intraventricular norepinephrine on preoptic-anterior hypothalamic electrical activity in the freely-moving rat: modulation by ovarian steroid hormones.

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    authors: Leung PC,Whitmoyer DI,Arendash GW,Sawyer CH

    更新日期:1981-12-07 00:00:00

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    journal_title:Brain research

    pub_type: 杂志文章


    authors: George SR,Kertesz M

    更新日期:1985-05-13 00:00:00

  • Evaluation of 5-ethynyl-2'-deoxyuridine staining as a sensitive and reliable method for studying cell proliferation in the adult nervous system.

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    authors: Zeng C,Pan F,Jones LA,Lim MM,Griffin EA,Sheline YI,Mintun MA,Holtzman DM,Mach RH

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    authors: Arjune D,Standifer KM,Pasternak GW,Bodnar RJ

    更新日期:1990-12-03 00:00:00

  • Distribution of somatostatin receptor subtypes in rat lumbar spinal cord examined with gold-labelled somatostatin and anti-receptor antibodies.

    abstract::Using gold-labelled somatostatin, somatostatin binding sites were predominantly found in laminae I-III, X and on motorneurones of the rat lumbar spinal cord. A comparison with immunohistochemical staining using antisera against somatostatin receptor sequences revealed that the marked binding in laminae I-III coincided...

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    pub_type: 杂志文章


    authors: Segond von Banchet G,Schindler M,Hervieu GJ,Beckmann B,Emson PC,Heppelmann B

    更新日期:1999-01-16 00:00:00

  • PWZ-029, a compound with moderate inverse agonist functional selectivity at GABA(A) receptors containing alpha5 subunits, improves passive, but not active, avoidance learning in rats.

    abstract::Benzodiazepine (BZ) site ligands affect vigilance, anxiety, memory processes, muscle tone and epileptogenic propensity through modulation of neurotransmission at GABA(A) receptors containing alpha1, alpha2, alpha3 or alpha5 subunits, and may have numerous experimental and clinical applications. The ability of non-sele...

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    pub_type: 杂志文章


    authors: Savić MM,Clayton T,Furtmüller R,Gavrilović I,Samardzić J,Savić S,Huck S,Sieghart W,Cook JM

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  • Effects of L-DOPA on neuronal activity of the globus pallidus externalis (GPe) and globus pallidus internalis (GPi) in the MPTP-treated monkey.

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    authors: Boraud T,Bezard E,Guehl D,Bioulac B,Gross C

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  • Blunted brain metabolic response to ketamine in mice lacking D(1A) dopamine receptors.

    abstract::The interaction of glutamatergic and dopamine neurotransmission is thought to have relevance to both the pathophysiology and pharmacotherapy of schizophrenia. For example, subanesthetic doses of the N-methyl-D-aspartate receptor (NMDA-R) antagonist ketamine induce schizophrenia-like behavioral effects in humans and bo...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Miyamoto S,Mailman RB,Lieberman JA,Duncan GE

    更新日期:2001-03-16 00:00:00

  • Sleep states and sleep electroencephalographic spectral power in mice lacking the beta 3 subunit of the GABA(A) receptor.

    abstract::Mice lacking the GABA(A) receptor beta(3) subunit exhibit a profound disruption in thalamic circuitry. We have studied sleep in these mice under baseline conditions and following treatment with the benzodiazepine midazolam. Under baseline conditions, NREM sleep time did not differ between beta(3) subunit knockout mice...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Wisor JP,DeLorey TM,Homanics GE,Edgar DM

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  • Short-lived diabetes in the young-adult ZDF rat does not exacerbate neuronal Ca(2+) biomarkers of aging.

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    journal_title:Brain research

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    authors: Maimaiti S,DeMoll C,Anderson KL,Griggs RB,Taylor BK,Porter NM,Thibault O

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  • Opiate-like excitatory effects of steroid sulfates and calcium-complexing agents given cerebroventricularly.

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    journal_title:Brain research

    pub_type: 杂志文章


    authors: LaBella FS,Havlicek V,Pinsky C

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  • CA1 ischemic injury does not affect the ability of Mongolian gerbils to solve response, direction, or place problems.

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    journal_title:Brain research

    pub_type: 杂志文章


    authors: Walsh SJ,Harley CW,Corbett D,Skinner DM,Martin GM

    更新日期:2008-01-02 00:00:00

  • Benzodiazepine receptor binding following chronic treatment with naloxone, morphine and met-enkephalin in normal rats.

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    journal_title:Brain research

    pub_type: 杂志文章


    authors: Rocha L,Tatsukawa K,Chugani HT,Engel J Jr

    更新日期:1993-05-28 00:00:00

  • Thyrotropin-releasing hormone potently reverses epinephrine-stimulated hyperglycemia in mice.

    abstract::Intracerebroventricular microinjection of thyrotropin-releasing hormone (TRH) potently blocked the development of, as well as promptly reversed, epinephrine-stimulated hyperglycemia in mice. The central antihyperglycemic effect was dose-related (0.1-10 micrograms), could be reproduced by an intravenous injection of a ...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Amir S,Harel M,Rivkind AI

    更新日期:1987-12-01 00:00:00

  • Distribution of glutamine synthetase and glial fibrillary acidic protein and correlation of glutamine synthetase with glutamate decarboxylase in different regions of the rat central nervous system.

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    journal_title:Brain research

    pub_type: 杂志文章


    authors: Patel AJ,Weir MD,Hunt A,Tahourdin CS,Thomas DG

    更新日期:1985-04-01 00:00:00

  • Effects of insulin, insulin-like growth factor-II and nerve growth factor on neurite outgrowth in cultured human neuroblastoma cells.

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    journal_title:Brain research

    pub_type: 杂志文章


    authors: Recio-Pinto E,Ishii DN

    更新日期:1984-06-08 00:00:00