Abstract：
:Pharmacological studies using an in vitro model system were carried out to determine if naloxone, an opiate receptor antagonist, could have effects on neuronal membranes which were unrelated to its action as an opiate receptor antagonist. Intracellular recordings were made from cultured mammalian spinal cord neurons. Putative amino acid neurotransmitters and naloxone were applied by iontophoresis or superfusion. When naloxone was co-iontophoresed with the amino acids a depression of the GABA response resulted. This depression was dose-dependent and reversible. At the lower doses of naloxone tested, the depression was specific since the glycine and glutamate responses were unaffected. At the higher doses of naloxone tested, alterations in the glycine and/or glutamate responses and membrane input resistance were frequently observed. The naloxone depression of the GABA response did not appear to involve opiate receptors since (+)-naloxone, the inactive isomer, equally depressed the GABA response. Analysis of the effect of naloxone on GABA dose-response curves indicates that naloxone acts as a competitive antagonist at the neuronal GABA receptors. Similar results were obtained when naloxone was applied by superfusion. However, high concentrations of naloxone (0.1-1 mM) were required, suggesting that naloxone has a low affinity for the GABA receptor. These data indicate that under some experimental conditions naloxone could not be considered a specific opiate antagonist.
journal_name
Brain Resjournal_title
Brain researchauthors
Gruol DL,Barker JL,Smith TGdoi
10.1016/0006-8993(80)90747-7subject
Has Abstractpub_date
1980-10-06 00:00:00pages
323-32issue
2eissn
0006-8993issn
1872-6240pii
0006-8993(80)90747-7journal_volume
198pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:The effects of noxious and non-noxious limb stimulations on the second exteroceptive suppression of voluntary temporalis muscle activity (ES2) were studied in healthy human volunteers. Duration of temporalis ES2 was measured on averaged rectified responses obtained after stimulating the labial commissure at an intensi...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)90970-9
更新日期：1994-09-19 00:00:00
abstract：:Different mechanisms have been suggested to contribute to isoflurane-mediated neuroprotection. Previous studies have suggested that the protein Slit can abrogate neuronal death in mixed neuronal-glial cultures exposed to oxygen-glucose deprivation (OGD) and reperfusion (OGD/R). We hypothesized that isoflurane increase...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2013.08.036
更新日期：2013-11-06 00:00:00
abstract：:Two different ligands, tritiated oxytocin and a newly synthesized and monoiodinated oxytocin antagonist, were used to reveal sites which bind oxytocin in the brainstem and upper spinal cord of 12 human subjects. Tissue sections were incubated with either ligand at a concentration close to their respective dissociation...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)90317-x
更新日期：1989-10-23 00:00:00
abstract：:Intracellular recordings from locus coeruleus neurons grown in explant cultures reveal age-related differences in the responses to iontophoretically applied noradrenaline. Cells grown in culture for more than 26 days exhibit a simple hyperpolarizing response while cells cultured for less than 26 days often have biphas...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(86)80238-4
更新日期：1986-03-01 00:00:00
abstract：:Two separate in vitro assays were used to examine the biosynthesis of the broad spectrum excitatory amino acid receptor antagonist kynurenic acid (KYNA) during the life span of the adult rat. Assessment of KYNA's anabolic enzyme kynurenine aminotransferase revealed steady increases between 3 and 24 months of age in al...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)91337-e
更新日期：1992-08-14 00:00:00
abstract：:Transient global cerebral ischemia has been shown to induce marked changes in the polyamine pathway with a significant increase in putrescine, the product of the ornithine decarboxylase reaction. This study examined the relationship between tissue and extracellular polyamines and regional cerebral blood flow and brain...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/S0006-8993(96)01116-X
更新日期：1997-01-09 00:00:00
abstract：:To assess the role of calcitonin gene-related peptide (CGRP) within the trigeminal subnucleus caudalis (Vc) on adrenal and autonomic function, microinjections were directed at different laminae of Vc in chloralose-anesthetized cats. Microinjections of CGRP (5 pmol, 100 nl) into laminae I-II increased significantly the...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)90713-6
更新日期：1991-08-30 00:00:00
abstract：:Extracellular and intracellular recordings have been made from non-pyramidal neurones in the rat hippocampus in vivo and in vitro. These cells were situated in the stratum pyramidale but were orthodromically activated with a lower threshold than pyramidal neurones in response to stimulation of the Schaffer collateral/...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)91235-6
更新日期：1984-02-20 00:00:00
abstract：:The aim of this study was to examine: (1) whether long-term potentiation (LTP) can be induced in slices from adult rat visual cortex under conditions where inhibition is not antagonized, and (2) the role of N-methyl-D-aspartate (NMDA) receptors in its induction. The field potential elicited in layer III in response to...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)90891-c
更新日期：1992-07-03 00:00:00
abstract：:We investigated the protective effect of subchronic treatment of the A2A receptor antagonist, SCH 58261 (0.01 mg/kg, i.p.), administered 5 min, 6 h and 15 h after permanent right middle cerebral artery occlusion (MCAo). Twenty-four hours after ischemia, an extensive pallid area, evaluated by cresyl violet staining, is...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2005.12.010
更新日期：2006-02-16 00:00:00
abstract：:In decerebrate cats, the extracellular activities of neurons in the medial medullary reticular formation were studied during constant velocity off-vertical axis rotations (OVAR) in the clockwise (CW) and counterclockwise (CCW) directions (at 10 degrees tilt). Spontaneously active neurons demonstrated sinusoidal positi...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(96)00516-1
更新日期：1996-09-02 00:00:00
abstract：:Administration of aminooxyacetic acid, (an inhibitor of gamma-aminobutyric acid (GABA)-transaminase which enhances the hypothalamic GABA content) increased luteinizing hormone (LH) and follicle stimulating hormone (FSH) levels in prepubertal rats (16 days of age), and decreased the levels of these pituitary hormones i...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)91508-x
更新日期：1991-11-01 00:00:00
abstract：:The present study was carried out in order to determine whether angiotensin II (ANG II) exerts its stimulatory effect on plasma concentration of arginine vasopressin (AVP) and adrenocorticotrophic hormone (ACTH) by interacting with a (gamma-aminobutyric acid) GABAergic pathway. For this purpose, the effect of the GABA...
journal_title：Brain research
pub_type： 临床试验,杂志文章
doi：10.1016/0006-8993(89)90100-5
更新日期：1989-07-03 00:00:00
abstract：:Ongoing electrical activity was recorded from the axons of neurosecretory cells from the brain of 5th instar Rhodnius prolixus throughout the moulting cycle. Dramatic changes in both the frequency and pattern of electrical activity occur at specific times during the cycle, enabling the timing of release of neurohormon...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(80)90314-5
更新日期：1980-06-02 00:00:00
abstract：:The origin of the afferents of the thalamic anterior paraventricular and parataenial nuclei (PVA-PT) in the rat was studied with fluorescent retrograde tracing method. After iontophoretic application of Fluoro-Gold into PVA-PT, many labeled cells were found in the medial frontal cortex, ventral subiculum, claustrum, s...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)91570-7
更新日期：1990-07-02 00:00:00
abstract：:Chronically epileptic male adult rats in the pilocarpine model of temporal lobe epilepsy (TLE), exhibited gross expansion of abdominal fat mass and significant weight gain several months after induction of status epilepticus (SE) when compared to control rats. We hypothesized that epileptogenesis can induce molecular ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2011.10.006
更新日期：2011-12-02 00:00:00
abstract：:This study tested the hypothesis that the short-latency excitation of the phrenic motor output elicited by superior laryngeal nerve (SLN) stimulation requires non-NMDA receptor-mediated neurotransmission in the region of the dorsal respiratory group (DRG) of the adult cat. Injection of the non-NMDA receptor antagonist...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)00355-t
更新日期：1995-06-05 00:00:00
abstract：:In anaesthetized cats, medial gastrocnemius motor units (MUs) were stimulated with random sequences (mean rates between 6 and 12 pps) of electrical pulses delivered to their axons in small ventral root filaments. Muscle tension was recorded under isometric conditions, and spike trains of muscle spindle afferents were ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)91656-2
更新日期：1986-02-19 00:00:00
abstract：:Nineteen convergent neurones, 19 'non-noxious only' neurones and 13 'proprioceptive' neurones all with peripheral excitatory receptive fields on the ipsilateral hindpaw, were recorded in the lumbar dorsal horn of non-spinalized, anaesthetized rats. These neurones were all excited by the electrophoretic application of ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)91147-8
更新日期：1984-04-23 00:00:00
abstract：:Overactivation of poly (ADP-ribose) polymerase-1 (PARP-1) in response to DNA damage is considered to play a crucial role in the development of post-ischemic neuronal injury, such as ischemic stroke. The present study was undertaken to clarify the beneficial effects of MP-124, a novel PARP-1 inhibitor, on neurological ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2011.05.069
更新日期：2011-09-02 00:00:00
abstract：:The trace elemental concentrations, including iron, in the substantia nigra (SN) of a 6-OHDA induced rat model of Parkinson's disease were measured using nuclear microscopy. Only rats that exhibited amphetamine induced rotation of more than 7 turns/min were used. The results showed that the iron levels were significan...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(96)00313-7
更新日期：1996-09-30 00:00:00
abstract：:Cytochrome P45011B1 (11 beta-hydroxylase) was detected in the brain of male rats by in situ hybridization methods. Normal Sprague-Dawley rats were compared to the transgenic strain TGR(mRen2)27, characterized by the expression of the murine Ren-2d renin gene and the development of severe hypertension. Specific ribopro...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(96)00540-9
更新日期：1996-09-09 00:00:00
abstract：:In anaesthetized cats, effects of iontophoretic application of angiotensin II were studied in the nuclei of the tractus solitarius. Specifically, its effects were tested on single respiratory neurones, having rhythmic activity related temporally with the phrenic nerve discharge, and on presumed reflex interneurones, w...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)91232-7
更新日期：1987-03-24 00:00:00
abstract：:Active substance P binding sites were solubilized from rat brain membranes by treatment with 0.125% sodium glycodeoxycholate and 1 M NaCl. About 50% of the binding activity in membrane-bound binding sites was recovered in the solubilized fraction after centrifugation at 105,000 g for 1 h. [3H]Substance P absorbed exte...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)91122-4
更新日期：1986-04-02 00:00:00
abstract：:Experiments were conducted to (1) provide further evidence of the selectivity of [D-Ala2,Leu5,Cys6]enkephalin (DALCE) as an antagonist of delta (delta) receptor ligands and (2) use DALCE as a tool to explore the possible role of delta receptors in restraint stress. Dose- and time-response curves were generated for the...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)90545-m
更新日期：1990-02-19 00:00:00
abstract：:In alert cats, extracellular spikes of neurons in the medial pontine tegmentum were recorded simultaneously with whole nerve discharges of the abducens and medial rectus nerves during horizontal vestibular nystagmus. Nystagmus-related neurons were classified by their firing patterns in relation to the abrupt cessation...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)90941-0
更新日期：1981-10-05 00:00:00
abstract：:Distribution and time course of the occurrence of "dark" neurons were compared with the EEG activity and behavior of rats during 4-aminopyridine (4-AP) induced epileptic seizures. A crystal of the K(+) channel blocker 4-AP (0.5 mg/kg) was placed onto the exposed parieto-occipital cortex of Halothane-anesthetized rats ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2008.06.044
更新日期：2008-09-04 00:00:00
abstract：:The lamination of the hippocampus in the homozygous B6CBA weaver mouse (wv/wv) was compared with that in normal B6CBA littermates (+/+) and C57BL/6J mice using Nissl and Timm's staining. In Nissl-stained preparations, the normal littermates exhibit a compact, regular arrangement of pyramidal cells in area CA3 of the h...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)00974-u
更新日期：1995-10-23 00:00:00
abstract：:Although transsynaptic tracing with peripheral injection of pseudorabies virus (PRV) has been extensively characterized, several methodological issues related to central application of this tracer have not been addressed. In the present study, we addressed the following three issues by using microinjection of a cockta...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(99)01680-7
更新日期：1999-08-14 00:00:00
abstract：:Noradrenaline effects on oxidative metabolism (tricarboxylic acid cycle activity) were examined by measuring the formation of 14CO2 from labeled aspartate in primary cultures of cerebral astrocytes and neurons and of cerebellar granule cells. At all time periods studied, CO2 formation in astrocytes was significantly i...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)91157-c
更新日期：1990-09-17 00:00:00
