The organization of intrinsic hippocampal connections in explants of rat hippocampus studied by topical application of HRP crystals.

Abstract:

:Hippocampal slices were taken from 7-day-old rats and maintained in vitro for 1-3 weeks. The organization of intrahippocampal connections in these explants was studied by placing onto the tissue small crystals of horseradish peroxidase (HRP) soaked with the detergent Nonidet. Antero- and retrograde transport of HRP was visualized by diaminobenzidine. The principal arrangement of intrinsic hippocampal connections closely resembles the in situ situation of the adult rat. The use of HRP crystals provides a fast and convenient tool for the study of connections in brain explants.

journal_name

Brain Res

journal_title

Brain research

authors

Keller F,Lipp HP,Waser PG

doi

10.1016/0006-8993(86)91448-4

subject

Has Abstract

pub_date

1986-08-13 00:00:00

pages

191-5

issue

1

eissn

0006-8993

issn

1872-6240

pii

0006-8993(86)91448-4

journal_volume

380

pub_type

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