Abstract：
:Astrocytic gap junctions have been implicated in a variety of signaling pathways essential to normal brain function. However, no information exists on the prevalence of gap junction channels and their function in the aging brain. Here we have compared the expression of the two most abundant astrocytic gap junction proteins in young and senescent brains and quantified the extent of functional gap junction coupling. The expression level of Cx43 peaked in 7-month-old mice. The relative numbers of Cx43 immunoreactive plaques were 596+/-61, 734+/-62, and 755+/-114 in 3-, 7-, and 21-month-old mice, whereas plaques size averaged 0.9+/-0.1 microm(2) (3 months), 1.3+/-0.1 microm(2) (7 months), and 0.7+/-0.1 microm(2) (21 months). The expression level of Cx30 was also highest in 7-month-old animals (315+/-49 plaques, size 0.8+/-0.07 microm(2) vs. 585+/-51 plaques, size 0.9+/-0.1 microm(2) in 3- and 7-month-old mice, respectively), but only 262+/-63 plaques (size 0.4+/-0.04 microm(2)) in 21-month-old mice. Western blot analysis revealed that the content of both Cx43 and Cx30 remained relatively constant at 3, 7, and 21 months. The fluorescence recovery of photobleach technique (FRAP) was used to evaluate coupling in freshly prepared hippocampal slices. Gap junction coupling did not change significantly as a function of aging, but a tendency towards reduced coupling was observed as the animals aged. Average fluorescence recovery after 2 min was 63+/-6% in younger animals, 59+/-5% in adult animals, and 54+/-4% in old brain. These observations indicate that although astrocytic gap junction proteins are maintained at high levels through the entire lifespan of mice, aging is associated with changes in the number and size of both Cx30 and Cx43 gap junction plaques.
journal_name
Brain Resjournal_title
Brain researchauthors
Cotrina ML,Gao Q,Lin JH,Nedergaard Mdoi
10.1016/s0006-8993(01)02258-2subject
Has Abstractpub_date
2001-05-18 00:00:00pages
55-61issue
1-2eissn
0006-8993issn
1872-6240pii
S0006-8993(01)02258-2journal_volume
901pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:The effects of adenosine and the adenosine binding enhancer, PD 81,723, on low magnesium-induced bursting in the in vitro hippocampal slice were examined. Extracellular recordings were obtained from the CA3 pyramidal cell layer while electrically stimulating in the stratum radiatum under low magnesium perfusion. Adeno...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)91604-q
更新日期：1993-08-13 00:00:00
abstract：:Selective D1 (SCH-23390) and D2 (eticlopride and sulpiride) dopamine receptor antagonists were assessed for their ability to reverse the effects of 1.0 mg/kg D-amphetamine on excitatory motor-related neurons in the striatum of freely moving rats. SCH-23390 (0.125, 0.25, 0.5 and 1.0 mg/kg) rapidly and consistently bloc...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)91633-4
更新日期：1993-08-13 00:00:00
abstract：:The so-called on- and off-cells of the rostral ventromedial medulla (RVM) send their axons to the spinal dorsal horn. Activation of on-cells precedes and coincides with a facilitation, and activation of off-cells coincides with an inhibition, of withdrawal reflexes elicited by noxious agents. Considerable evidence sup...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2018.02.025
更新日期：2018-05-01 00:00:00
abstract：:The organization of globus pallidus (GP) projections was studied in cats using autoradiographic and horseradish peroxidase (HRP) techniques. Both methods confirmed the existence of a topographically organized projection to subthalamic nucleus (STN). Although all but the most caudal GP projects to STN, the heaviest pro...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(80)90003-7
更新日期：1980-05-05 00:00:00
abstract：:To determine whether post-traumatic seizure severity would be affected by the interval between seizures and head injury, we measured seizures after various times with or without fluid percussion brain injury (2atm fluid percussion injury; FPI). To determine efficacy of anti-seizure medication, we also determined if le...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2016.04.013
更新日期：2016-07-01 00:00:00
abstract：:The behavioral and EEG effects of rat and ovine corticotropin releasing factor (r- and o-CRF) were compared. Both peptides were injected intracerebroventricularly into rats through chronically implanted cannulae. At the doses of 0.1 and 1 microgram both peptides activated the EEG and stimulated motor activity. At the ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)90413-6
更新日期：1987-04-07 00:00:00
abstract：:The effects of the serotonin precursor 5-HTP, were determined in a behavioral DNIC paradigm (increase in vocalization threshold after intraperitoneal injection of the algogenic agent, phenylbenzoquinone). This counter-irritation phenomenon was strongly potentiated by 5-HTP, such potentiation being blocked by the 5-HT ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(82)90384-5
更新日期：1982-01-14 00:00:00
abstract：:The central myelin sheaths of the recessive autosomal mutant mouse, Shiverer, were investigated by electron microscopy with special reference to the pattern of myelin formation. The results obtained may be summarized as follows: (1) the major dense line of myelin lamellae generally failed to be formed, but the axons w...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)90269-9
更新日期：1981-08-24 00:00:00
abstract：:Two distinct families of low-molecular-weight toxins (argiotoxins) have been isolated from the venom of the orb-web spider. Argiope trifasciata. The toxins have been purified to homogeneity and characterised by spectroscopic, mass spectrometric and microchemical analysis. The major biologically active member of the fi...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)91098-0
更新日期：1988-05-10 00:00:00
abstract：:In intracellular recordings from the CA1 region of the hippocampal slice preparation, an infrequent cell type different from the predominant pyramidal cell has been characterized. The cells have very brief spikes, hyperpolarizing after-potentials, and tend to fire at a high spontaneous rate; unitary synaptic potential...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(78)90991-5
更新日期：1978-11-17 00:00:00
abstract：:Using event-related potentials (ERPs), this study investigated the neuromechanism underlying verbal analogical reasoning of two different metaphorical relations: attributive metaphor and relational metaphor. The analogical reasoning of attributive metaphor (AM-AR) involves a superficial similarity between analogues, w...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2011.09.041
更新日期：2011-11-24 00:00:00
abstract：:This review examines vestibular compensation and vestibular rehabilitation from a unified translational research perspective. Laboratory studies illustrate neurobiological principles of vestibular compensation at the molecular, cellular and systems levels in animal models that inform vestibular rehabilitation practice...
journal_title：Brain research
pub_type： 杂志文章,评审
doi：10.1016/j.brainres.2012.08.040
更新日期：2012-10-30 00:00:00
abstract：:Hemicholinium-3 (HCh-3) is a potent and specific inhibitor of the high-affinity choline transport process (HAChT) localized on cholinergic neurons. In this study, the specific binding of [3H]HCh-3 (120 Ci/mmol) was characterized in crude synaptic membranes prepared from rat brain. The binding of [3H]HCh-3 to forebrain...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(85)90451-2
更新日期：1985-12-02 00:00:00
abstract：:Catecholamine concentrations were determined at central and peripheral level in high blood pressure (HBP), low blood pressure (LBP) and random bred (RB) mice. In HBP mice compared to LBP, the noradrenaline concentration was lower in the locus coeruleus, medullary A1-C1 and A2-C2 areas, thoracic spinal cord, posteroven...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(85)90784-x
更新日期：1985-08-05 00:00:00
abstract：:Seizurogenic activity develops in many patients following brain injury and may be involved in the pathophysiological effects of brain trauma and stroke. We have evaluated the effects of the use-dependent sodium channel blocker RS100642, an analog of mexiletine, as a neuroprotectant and anti-seizure agent in a rat mode...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)02275-8
更新日期：2002-04-05 00:00:00
abstract：:These experiments were conducted in order to better understand the role of catecholaminergic neurons in intracranial self-stimulation behaviour (ICSS) elicited from the lateral hypothalamus (LH). Two strains of mice which differ in their ICSS rate and their thresholds were studied. In a first time, we compared the cat...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(83)90341-4
更新日期：1983-04-18 00:00:00
abstract：:Following lesions of the pyramidal tract in hamsters, retrograde changes were studied in the sensorimotor cortex and in the pyramidal tract axons proximal to the lesion, at survival times ranging from 2 weeks to 14 months. Severe cell shrinkage occurred in layer 5 pyramidal neurons as early as 2 weeks, but there was n...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(75)90130-4
更新日期：1975-05-16 00:00:00
abstract：:Responses of subthalamic neurons (STN) to the iontophoretic application of glutamate (Glu), acetylcholine (ACh) and GABA were studied in rats 2 or 3 weeks following bilateral decortication, and were compared to those obtained in normal animals. All the cells recorded in lesioned rats showed a decrease in their spontan...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)90077-1
更新日期：1987-02-17 00:00:00
abstract：:Cerebrospinal fluid (c.s.f.) pressure and resistance to absorption have been studied in exteriorized fetuses (18 to 21 days gestation) from pregnant rats anaesthetised with pentobarbitone and in similarly anaesthetised postnatal rats at 1, 5, 10 and 30 days after birth and in adults. The resting c.s.f. pressure was lo...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(87)90172-6
更新日期：1987-05-01 00:00:00
abstract：:As measured by extracellular single-cell recording, the responses to adenosine triphosphate (ATP) by ATP-sensitive chemoreceptors (ATP cells) on the olfactory organ of the spiny lobster are markedly suppressed by adenosine diphosphate (ADP), adenosine monophosphate (AMP) and to a lesser extent, adenosine, when each is...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)90964-5
更新日期：1989-09-11 00:00:00
abstract：OBJECTIVE:This study aimed to investigate whether β-amyloid (Aβ) was able to enhance neurocan expression in a Sox9 dependent manner in astrocytes. METHODS AND MATERIALS:Astrocytes were incubated with Aβ at different concentrations, the expression of Sox9 and neurocan was detected by Western blot assay. Meanwhile, the ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2016.06.010
更新日期：2016-09-01 00:00:00
abstract：:We recently reported raphe neurones which frequently fired spikes in short bursts. However, the action potentials were broad and the neurones fired in a slow and regular pattern, suggesting they were an unusual type of 5-hydroxytryptamine (5-HT) neurone. In the present study, we investigated whether these putative bur...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(96)00869-4
更新日期：1996-11-18 00:00:00
abstract：:Anisomycin is both a well-established protein synthesis inhibitor and a potent activator of the p38/JNK MAPK pathway. It has been used to block the late phase of long-term potentiation (LTP) and long-term depression (LTD) in hippocampus. In this study, we have found that anisomycin produces a time-dependent decline in...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2006.02.015
更新日期：2006-04-26 00:00:00
abstract：:The cat substantia nigra (SN) pars reticulata (SNR) has been observed electron microscopically after ibotenic acid injections into the caudate nucleus (Cd) and horseradish peroxidase injections into the nucleus tegmenti pedunculopontinus pars compacta (TPC). As a result, degenerating striatal terminals were found to m...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)90683-5
更新日期：1989-04-17 00:00:00
abstract：:Type 1 cannabinoid (CB1) receptors are expressed in high concentrations in the central nervous system, including the basal ganglia, and could have direct or indirect effects on motor behavior through modulation of dopaminergic, glutamatergic and GABA-ergic neurotransmission. Using the CB1 receptor radioligand [(18)F]M...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2009.12.026
更新日期：2010-02-26 00:00:00
abstract：:Two distinct serotonin receptors in mammalian brain are labeled respectively with [3H]serotonin (5-HT1) and [3H]spiperone (5-HT2). In general agonists display highest affinities for 5-HT1 while antagonists prefer 5-HT2 sites. To conduct regional studies of 5-HT receptors, we estimated 5-HT2 sites with [3H]spiperone, u...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)90562-x
更新日期：1981-03-16 00:00:00
abstract：:The respiratory role of GABA(A), GABA(B) and glycine receptors within the Bötzinger complex (BötC) and the pre-Bötzinger complex (preBötC) was investigated in alpha-chloralose-urethane anesthetized, vagotomized, paralysed and artificially ventilated rabbits by using bilateral microinjections (30-50 nl) of GABA and gly...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.05.032
更新日期：2010-07-16 00:00:00
abstract：:The subfornical organ of the brain has a role in the regulation of fluid balance in higher animals. In this study the effects of salt loading and water deprivation on specific proteins in this organ were investigated. For 4 days, 3 groups of rats were given an appropriate fluid diet (control, 2% NaCl and water deprive...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)91463-0
更新日期：1986-04-30 00:00:00
abstract：:The blockade of central histaminergic H(2) receptors has been reported to aggravate ischemic neuronal damage. Since excess release of excitatory neurotransmitters is closely related to ischemic neuronal damage, the effects of ranitidine on ischemic release of dopamine were investigated in gerbil striatum. Changes in t...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(01)03107-9
更新日期：2002-02-01 00:00:00
abstract：:Previous reports from this laboratory have indicated that fetal rat striatal grafts have the major types of neuronal and glial components known to be involved in Huntington's chorea. In this study a number of major afferent and efferent innervations seen in normal striatum were examined in the striatal grafts and were...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)91592-8
更新日期：1989-12-11 00:00:00
