Abstract：
OBJECTIVE:This study aimed to investigate whether β-amyloid (Aβ) was able to enhance neurocan expression in a Sox9 dependent manner in astrocytes. METHODS AND MATERIALS:Astrocytes were incubated with Aβ at different concentrations, the expression of Sox9 and neurocan was detected by Western blot assay. Meanwhile, the viability and proliferation of astrocytes were assessed by MTT assay. Then, the Sox9 expression was silenced, and the expression of Sox9 and neurocan was examined. RESULTS:After incubation with Aβ, the viability of astrocytes was increased regardless silencing of Sox9 (all P<0.05). The proliferation of astrocytes was also gradually increased with the increase in the time of Aβ incubation (all P<0.05). With the increase in Aβ concentration, the expression of Sox9 and neurocan was also increased (all P<0.05). However, after silencing of Sox9 expression, the neurocan expression was significantly reduced as compared to control group and scra-siRNA group (all P<0.05). CONCLUSION:Our study shows the viability and proliferation of astrocytes are significantly increased by Aβ in a dose dependent manner. Moreover, Aβ may effectively up-regulate the neurocan expression via regulating Sox9.
journal_name
Brain Resjournal_title
Brain researchauthors
Yan H,Zhu X,Xie J,Zhao Y,Liu Xdoi
10.1016/j.brainres.2016.06.010subject
Has Abstractpub_date
2016-09-01 00:00:00pages
377-383eissn
0006-8993issn
1872-6240pii
S0006-8993(16)30434-6journal_volume
1646pub_type
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