Abstract:
:The quantification of human immunodeficiency virus type 1 (HIV-1) RNA or hepatitis C virus (HCV) RNA has been facilitated by adapting a spin column procedure for sample preparation and the use of chemiluminescent detection of polymerase chain reaction (PCR) products in microtiter plate format. All materials were commercially available and relatively inexpensive. By making a single dilution prior to amplification, concentrations of 500 copies to 2.5 million HIV-1 1 RNA copies per mL and 1,000 copies to 50 million HCV RNA copies per mL could be determined on 140-microL samples. Between-run imprecision employing the improved procedure for HIV-1 RNA was 23%. Correlation of HIV-1 RNA concentrations obtained using chemiluminescent detection with values obtained by colorimetric assay of PCR products was 0.98. Correlation of HCV RNA concentration determined by the spin column-chemiluminescent assay procedure with those obtained by branched DNA methodology was 0.91. Spin columns could be used with serum or plasma containing acid-citrate-dextrose or heparin anticoagulant, but heparinized samples required treatment with heparinase prior to amplification.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Lin HJ,Tanwandee T,Hollinger FBsubject
Has Abstractpub_date
1997-01-01 00:00:00pages
56-63issue
1eissn
0146-6615issn
1096-9071pii
10.1002/(SICI)1096-9071(199701)51:1<56::AID-JMV9>3journal_volume
51pub_type
杂志文章abstract::The transmission of the severe acute respiratory syndrome corona virus 2 (SARS-CoV-2) can occur through airborne route, in addition to contaminated surfaces and objects. In hospitals, it has been confirmed by several studies that SRAS-CoV-2 can contaminate surfaces and medical equipment especially in hospitals dedicat...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26819
更新日期:2021-01-26 00:00:00
abstract::Although there is a low prevalence rate (around 1% of the population) of infection with hepatitis B virus (HBV) and hepatitis C virus (HCV) in Chile, little is known about the diversity and molecular characteristics of the circulating viruses. In the present study, 40 HBV and 57 HCV samples from Santiago City, Chile, ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21607
更新日期:2009-11-01 00:00:00
abstract::The current study aimed to investigate the diagnostic value of glycated albumin (GA), glycated hemoglobin (HbA1c), and a number of routine biomarkers as noninvasive indicators of liver fibrosis in patients with chronic hepatitis C (CHC). One hundred patients with CHC were subjected to full medical history and examinat...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26204
更新日期:2020-06-19 00:00:00
abstract::The prevalence study and the characterization of hepatitis C virus (HCV) was carried out in the Philippines and the sequence determination of the 5'-untranslated region (5'-UTR)-Core and the NS5B regions of HCV was carried out in this study. An HCV strain (SE-03-07-1689) collected in Metro Manila, Philippines, belonge...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20714
更新日期:2006-11-01 00:00:00
abstract::From November 2004 to April 2007, specimens were obtained from 2,281 patients with acute respiratory tract illness in Normandy, France. Eighteen strains of influenza C virus were detected in these samples using a combined tissue culture/RT-PCR diagnostic method. Most patients with influenza C virus infection (13/18) w...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21218
更新日期:2008-08-01 00:00:00
abstract::Previous studies on patients who develop drug resistant HIV-1 variants have shown that continued use of failing regimens might provide clinical benefit. However, the effect of long-term exposure to drug resistant variants may lead to emergence of compensatory mutations that may jeopardize this effect. In this study, w...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20582
更新日期:2006-05-01 00:00:00
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journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22042
更新日期:2011-05-01 00:00:00
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journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20029
更新日期:2004-04-01 00:00:00
abstract::WU and KI polyomaviruses (WUPyV, KIPyV) have been detected in respiratory, blood, stool, and lymphoid tissue, but not in urine samples. PCR based detection revealed higher frequency in immunocompromised individuals. In this study the prevalence of WUPyV and KIPyV was analyzed in respiratory, urine, and blood samples f...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.22083
更新日期:2011-07-01 00:00:00
abstract::Saliva and peripheral blood samples from 20 healthy adults were examined for the presence of human herpesvirus-6 (HHV-6) DNA sequences using the polymerase chain reaction. Eighteen out of 20 whole saliva samples contained detectable HHV-6 genomes. The majority of peripheral blood samples were also positive; however, t...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890320113
更新日期:1990-09-01 00:00:00
abstract::Rapid PCR-based influenza tests are increasingly used as point-of-care diagnostics in hospitals and clinics. To our knowledge, no prior studies have described clinical outcomes with implementation of rapid PCR-based influenza tests in hospitalized adult inpatients. Electronic medical records were used to assess differ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24279
更新日期:2015-12-01 00:00:00
abstract::Epstein-Barr virus (EBV) is an important DNA tumor virus that is associated with approximately 10% of gastric carcinomas and 99% of nasopharyngeal cancers (NPC). DNA methylation and microRNAs (miRNAs) are the most studied epigenetic mechanisms that can prompt disease susceptibility. This study aimed to detect the effe...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24863
更新日期:2017-10-01 00:00:00
abstract::It has been reported that a new pattern of mutations, E44D/A and/or V118I, in the reverse transcriptase (RT) gene of HIV-1 confers a moderate level of resistance to lamivudine in the absence of the M184V mutation. The prevalence of this mutational pattern was studied in HIV-1 isolates obtained from 280 patients. These...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.2145
更新日期:2002-03-01 00:00:00
abstract::The relationships between host immune factors and HIV-1 disease progression are still in dispute. The RANTES SNPs exhibit distinct ethnic distribution and are associated with different effects on the course of HIV infection. Therefore, impact of RANTES gene polymorphism on HIV-1 transmission and progression needs to b...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21201
更新日期:2008-07-01 00:00:00
abstract::Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne disease caused by the Crimean-Congo hemorrhagic fever virus. Long non-coding RNAs (lncRNAs) are generally classified as transcripts longer than 200 nucleotides (nt). The various lncRNAs expressed in infected cells are responsible for regulating the expression of v...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25264
更新日期:2018-12-01 00:00:00
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journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10355
更新日期:2003-05-01 00:00:00
abstract::A recent cohort study1 at Suizhou in China identified that the proportion of subjects who wore eyeglasses was lower in hospitalized patients with coronavirus disease 2019 (COVID-19) than in the general population. This article is protected by copyright. All rights reserved. ...
journal_title:Journal of medical virology
pub_type: 信件
doi:10.1002/jmv.26845
更新日期:2021-02-02 00:00:00
abstract::In the ongoing coronavirus disease 2019 (COVID-19) pandemic, one potential cause of concern is that some discharged COVID-19 patients are testing positive again for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA. To better understand what is happening and to provide public health policy planners and ...
journal_title:Journal of medical virology
pub_type: 杂志文章,评审
doi:10.1002/jmv.26250
更新日期:2020-07-01 00:00:00
abstract::This is a case study of a child who developed roseola infantum first, then varicella, and was later affected by acute cerebellar syndrome, severe truncal ataxia, and myoclonic dystonia. Human herpesvirus 6 (HHV-6) A and B were detected in the cerebrospinal fluid (CSF) and peripheral blood, respectively, upon ataxia on...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20285
更新日期:2005-03-01 00:00:00
abstract::In addition to the serotype-specific primers described previously (1 to 7), a new serotype 8-specific primer has been designed, allowing detection of all astrovirus serotypes. A total of 1,382 diarrheal stool samples in 5 regions in Japan were examined by reverse transcription-polymerase chain reaction (RT-PCR). The i...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:2000-07-01 00:00:00
abstract::Human metapneumovirus (hMPV) has been described as circulating among the Uruguayan population at least since 1998 based on serologic evidence. However, no isolation attempts, molecular detection, or genetic studies have been carried out so far in the country. In the present study, molecular detection of circulating hM...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21752
更新日期:2010-05-01 00:00:00
abstract::Response to ribavirin therapy (1,000-1,200 mg/day for 6 months) was evaluated in nine patients with chronic hepatitis C virus (HCV) infections who had previously failed to respond to a 6-month course of alpha-interferon. All had chronic active hepatitis with elevated serum aminotransferase activities (mean +/- SD = 13...
journal_title:Journal of medical virology
pub_type: 临床试验,杂志文章
doi:10.1002/jmv.1890450106
更新日期:1995-01-01 00:00:00
abstract::There are limited reports on the etiology of multiple enteric viruses causing acute gastroenteritis (AGE) in North India. In the present study we have determined the prevalence of three enteric viruses, namely rotavirus, astrovirus (AstV) and adenovirus (AdV) in a total of 312 diarrheic children (<5 years) hospitalize...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25645
更新日期:2020-08-01 00:00:00
abstract::Hepatitis C virus (HCV) genotypes help to tailor the treatment response, but their influence on the disease severity and association with hepatic steatosis is not well understood. The prevalence of HCV genotypes and their correlation with the histopathological severity of liver disease and steatosis in Indian patients...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20561
更新日期:2006-04-01 00:00:00
abstract::The metastasis status of pelvic lymph nodes (PLNs) seems to be a predictive factor of survival. It was suggested that the presence of HPV DNA and other biological markers in PLN may indicate a sub clinical early metastasis. The aim was to describe the prevalence and distribution patterns of HPV DNA and H-ras mutations...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21076
更新日期:2008-04-01 00:00:00
abstract::Five temperature-sensitive mutants (tsm9, tsm13, tsm20, tsm22, tsm30) of murine cytomegalovirus have been shown previously not to produce infectious virus in mice. In the present study, the stage at which these mutants are blocked in their replication in vitro was examined by transcriptional analysis of 4 temporally r...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/1096-9071(200010)62:2<127::aid-jmv2>3.0.co
更新日期:2000-10-01 00:00:00
abstract:PROBLEM:Objective studies that use validated questionnaires are needed to evaluate the changes in the sexual functions of women diagnosed with human papillomavirus (HPV) infection. METHOD OF STUDY:The study comprised 80 sexually active women diagnosed with a high-risk HPV infection. These patients were divided into fo...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25623
更新日期:2020-08-01 00:00:00
abstract::To determine the seroprevalence of antibodies against dengue virus (DENV) and West Nile virus (WNV) in the human population of the Bolivian Chaco, we tested 256 inhabitants of two rural communities. The seroprevalence, confirmed by plaque reduction neutralization test, was 7.8% and 2.7% for DENV and WNV, respectively....
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25283
更新日期:2019-01-01 00:00:00
abstract::Junin virus infection in guinea pigs is known to be similar to human Argentine hemorrhagic fever (AHF). The guinea pig was chosen as a model for transplacental transmission of Junin virus, as both guinea pig and man have a similar placental structure. Pregnant guinea pigs were infected with the pathogenic XJ strain of...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890110210
更新日期:1983-01-01 00:00:00
abstract::The 30-base pair (bp) deletion of the cytoplasmic carboxy-terminal domain of the latent membrane protein 1 (LMP-1) gene was analyzed in 37 frozen tissues from patients with Epstein-Barr virus (EBV)-related gastric cancer and 18 throat washings from healthy adults in southern Japan. The 30-bp deletion was identified in...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(199902)57:2<152::aid-jmv11
更新日期:1999-02-01 00:00:00