Abstract:
:Rapid PCR-based influenza tests are increasingly used as point-of-care diagnostics in hospitals and clinics. To our knowledge, no prior studies have described clinical outcomes with implementation of rapid PCR-based influenza tests in hospitalized adult inpatients. Electronic medical records were used to assess differences in laboratory testing time and antiviral use among a subset of 175 consecutive adult inpatients tested for influenza in two respiratory seasons before and after implementation of rapid PCR-based influenza testing at an academic medical center. Of the 350 hospitalized inpatients included in this analysis, 96 (27%) were over 65 years of age and 308 (88%) had a comorbid condition. The overall time to result decreased significantly from 25.2 to 1.7 hr (P < 0.001) after implementation of rapid PCR-based influenza testing. Among influenza-negative patients, the frequency of oseltamivir initiation remained unchanged (before: 43% vs. after: 45%; P = 0.60), though the median duration of oseltamivir was significantly decreased from 1.1 to 0.0 days (P < 0.001). By providing an earlier result to clinicians, rapid PCR-based influenza tests may decrease unnecessary antiviral use among adult inpatients who test negative for influenza.
journal_name
J Med Viroljournal_title
Journal of medical virologyauthors
Chu HY,Englund JA,Huang D,Scott E,Chan JD,Jain R,Pottinger PS,Lynch JB,Dellit TH,Jerome KR,Kuypers Jdoi
10.1002/jmv.24279subject
Has Abstractpub_date
2015-12-01 00:00:00pages
2021-6issue
12eissn
0146-6615issn
1096-9071journal_volume
87pub_type
杂志文章abstract::A single rotavirus strain causing asymptomatic infections as well as severe gastrointestinal disease has been described in the neonatal nurseries of the Christian Medical College, Vellore. In this study, quantitative real-time RT-PCR was used to determine the association of viral load with the presence of gastrointest...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21872
更新日期:2010-10-01 00:00:00
abstract::Previous studies demonstrated that in vitro infection with influenza A viruses altered several functions of human monocytes-macrophages but did not detectably alter functions of human lymphocytes. However, both types of cells were infected, as determined by production and surface expression of viral antigens. In the c...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890270302
更新日期:1989-03-01 00:00:00
abstract::An enzyme-linked immunosorbent assay (ELISA) test using polyvalent antigens and antisera was used to detect Coxsackie B virus-specific IgM responses in 329 patients admitted to the Coronary Care Unit, Wellington Hospital, New Zealand over a 12-month period. The sera of 30 of 153 (19.6%) patients with acute myocardial ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890180211
更新日期:1986-02-01 00:00:00
abstract::Although cytomegalovirus (CMV) has been detected in the inner ear fluid of patients who succumbed to the complications of symptomatic congenital CMV infection, it has not been detected in the inner ear fluid of living patients. In this study, real-time polymerase chain reaction (PCR) was used to measure CMV DNA in cli...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10263
更新日期:2003-01-01 00:00:00
abstract::In vitro incubation of human cytomegalovirus (Towne strain) with 8 U/ml human recombinant myeloperoxidase plus sodium chloride and glucose nearly abolished viral infectivity. To assay the effect on intracellular infection, cell toxicity of the enzymes was first studied. Even the high dose of 16 U/ml of recombinant mye...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:
更新日期:2002-02-01 00:00:00
abstract::One hundred twenty strains of adenovirus types 1, 2, 5, and 6 (subgenus C), isolated at various times and places, were tested by neutralization and hemagglutination inhibition with two antisera of each type. Except for one type 5 strain they reacted largely type-specifically in both tests and neither qualitative nor q...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890180310
更新日期:1986-03-01 00:00:00
abstract::Recent studies have suggested a pathogenic role of human parvovirus B19 (B19) in the development of acute fulminant liver failure in children. The hypothesis was based on the detection of B19 DNA in 8 of 10 explanted livers of children requiring liver transplantation. In the present study, explanted livers from 43 adu...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.2047
更新日期:2001-10-01 00:00:00
abstract:BACKGROUND:Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA was found in the intestines and feces, but its clinical significance is not completely clear. We aim to characterize the longitudinal test results of SARS-CoV-2 RNA in anal swabs and to explore the association with disease severity. METHODS:We...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.26307
更新日期:2021-02-01 00:00:00
abstract::HIV diagnosis at the point-of-care or in resource-limited settings poses considerable challenges due to time and cost limitations. Currently, nucleic acid-based tests are the only reliable method for diagnosing recent infections during the window period post-infection and pre-seroconversion, but these tests are only s...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21490
更新日期:2009-06-01 00:00:00
abstract::The presence of the E2 region of HPV 16 was determined by the polymerase chain reaction in biopsy samples with different histopathological diagnoses. The absence of the E2 gene was observed in 17 of 30 cases examined. The E2 deletions were not confined exclusively to invasive carcinomas or to integrated viral forms. I...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890410102
更新日期:1993-09-01 00:00:00
abstract::In order to evaluate the true immune status and the effect of revaccination on a young adult population, we collected serum samples from 289 military recruits who were vaccinated during an outbreak in 1991. Most vaccinees, age 18-25 years, had apparently been immunized once before as infants. Sera collected just prior...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(SICI)1096-9071(199611)50:3<249::AID-JMV7>
更新日期:1996-11-01 00:00:00
abstract::Despite the success of antivirals in preventing clinically overt CMV disease in cardiac allograft recipients, sub-clinical active CMV infection remains a major concern because of its association with allograft rejection and vasculopathy. The measurement of CMV specific T-cell responses is a promising approach to asses...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21229
更新日期:2008-09-01 00:00:00
abstract::Enterovirus 68 (EV-D68) was associated with mild to severe respiratory infections. In the last 4 years, circulation of different EV-D68 strains has been documented worldwide. In this study, the phylogenetic characterization of nine EV-D68 strains identified in patients in the 2010-2012 period and 12 additional EV-D68 ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23821
更新日期:2014-09-01 00:00:00
abstract::Infection with oncogenic human papillomavirus (HPV) genotypes is necessary for the development of cervical cancer. Testing for HPV DNA from liquid based cervical samples can be used as an adjunct to traditional cytological screening. In addition there are ongoing viral load, genotyping, and prevalence studies. Therefo...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23882
更新日期:2014-04-01 00:00:00
abstract::Hepatitis C virus (HCV) variation in specific T-cell epitopes may represent a mechanism of viral persistence in chronic infection. We examined the HCV non-structural protein 3 (NS3), including the immunologically relevant epitopes HCV NS3-2 KLVALGINAV (human leukocyte antigen [HLA]-A2-restricted) and HCV NS3-1391 LIFC...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20036
更新日期:2004-04-01 00:00:00
abstract::Serial determinations of titers of binding antibodies to single stranded DNA were performed over a period of three years in 43 type B hepatitis patients with persisting HBsAg who had either developed chronic hepatitis or were asymptomatic carriers. Patients with histopathological diagnosis of chronic active or chronic...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890040204
更新日期:1979-01-01 00:00:00
abstract::A molecular epidemiological study on common diarrheal viruses was conducted in Ho Chi Minh City, Vietnam between October 2002 and September 2003. Fecal samples were collected from 1,010 hospitalized children with acute gastroenteritis. Those samples were screened for groups A, B, and C rotavirus, adenovirus, genogroup...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20857
更新日期:2007-05-01 00:00:00
abstract:BACKGROUND:The data on the seasonality of respiratory viruses helps to ensure the optimal vaccination period and to monitor the possible outbreaks of variant type. OBJECTIVES:This study was designed to describe the molecular epidemiology and seasonality of acute respiratory infection (ARI)-related respiratory viruses ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.25464
更新日期:2019-08-01 00:00:00
abstract::The TD97 strain vaccine virus was prepared from the Tanabe strain measles virus by low-temperature passages in primary cell cultures and ultraviolet (UV) mutagenesis. The TD97 strain exhibited the following characteristics: highly temperature sensitive, neither multiplying nor forming any plaques at 40 degrees C in Ve...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890320311
更新日期:1990-11-01 00:00:00
abstract::Co-infection with hepatitis delta virus (HDV) and hepatitis B virus (HBV) has been shown to be associated with a more severe form of acute and chronic hepatitis. Cloning and expression of recombinant HDV antigen (rHDAg) in Escherichiacoli are described. Using purified rHDAg, a cost-effective indirect anti-HDV enzyme-l...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23212
更新日期:2012-03-01 00:00:00
abstract::We have attempted to reactivate human herpesvirus 6 (HHV-6) by infection with HHV-7 using childhood exanthem subitum patients in vitro. Peripheral blood mononuclear cells (PBMCs) were collected from children who had a history of exanthem subitum(ES) by HHV-6 and were infected by human herpesvirus 7 (HHV-7) in vitro. T...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/(sici)1096-9071(200003)60:3<284::aid-jmv6>
更新日期:2000-03-01 00:00:00
abstract::Influenza A(H1N1)pdm09 virus pandemic struck India in 2009 and continues to cause outbreaks in its post-pandemic phase. Diminutive information is available about influenza A(H1N1)pdm09 from central India. This observational study presents epidemiological and molecular findings for the period of 6 years. Throat swab sa...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.24982
更新日期:2018-03-01 00:00:00
abstract::The JC virus (JCV) is a common human virus persisting in renal tissue. In immunocompromised individuals it may reactivate and cause progressive multifocal leukoencephalopathy (PML). JCV has also been implicated in cancerogenesis, leading to various brain tumors and cancers of gastrointestinal tract. In this study the ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.21153
更新日期:2008-06-01 00:00:00
abstract::A patient presented with acute retinal necrosis of the left eye. Demonstration of herpes simplex virus (HSV) DNA in the aqueous humour confirmed the diagnosis. Negative results of HSV type-specific antibody tests based on gG antigens suggested a primary HSV infection. However, the patient had a past history of laborat...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.20083
更新日期:2004-06-01 00:00:00
abstract::Among 39,656 voluntary blood donors in Okinawa Prefecture, Japan, 115 (0.29%) were repeatedly reactive for antibody to hepatitis C virus (anti-HCV) by second generation (2nd-gen) passive hemagglutination assay (PHA). Positive serum samples were tested for anti-HCV using three different enzyme immunosorbent assays (ELI...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890460408
更新日期:1995-08-01 00:00:00
abstract::Human rhinovirus (HRV), a single-stranded, positive-sense RNA virus, is associated with mild upper respiratory tract infections in children. The aim of this study was to carry out a molecular characterization and phylogeny-based classification of the circulating genotypes of HRV in hospitalized children with clinical ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.23638
更新日期:2013-09-01 00:00:00
abstract::Epstein-Barr virus is present in the saliva of most persistently infected individuals and is generally thought to be spread by close oral contact. However, there are now several reports of EBV in genital secretions, suggesting the possibility of sexual transmission between adults. The present study was undertaken to i...
journal_title:Journal of medical virology
pub_type: 杂志文章,多中心研究
doi:10.1002/jmv.20682
更新日期:2006-09-01 00:00:00
abstract::This open, randomized study was conducted in healthy Chinese youngsters, aged between 10 and 19 years to compare the reactogenicity and immunogenicity of two vaccines: the combined vaccine against hepatitis A and B was administered in a two-dose schedule with the profile of the corresponding monovalent vaccines, while...
journal_title:Journal of medical virology
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1999-09-01 00:00:00
abstract::The aim of the study was to assess the prevalence of human papillomavirus (HPV) in the esophagus in the coastal region of Eastern Guangdong, Southern China, an area with a high incidence of esophageal carcinoma. Fresh surgical resection esophageal specimens were obtained from 176 esophageal carcinoma patients admitted...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.10219
更新日期:2002-11-01 00:00:00
abstract::The effect on cellular (c) oncogene RNA levels was investigated after infection of permissive cells with cell culture adapted strains (AD-169, C-87, Davis) and unadapted clinical isolates (82-1, 84-2, 85-1) of human cytomegalovirus (HCMV). The results indicate that both adapted and unadapted strains of HCMV induce sub...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890340409
更新日期:1991-08-01 00:00:00